Experiment: Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS09725 and HGI48_RS09730 are separated by 6 nucleotides HGI48_RS09730 and HGI48_RS09735 overlap by 1 nucleotides HGI48_RS09735 and HGI48_RS09740 are separated by 14 nucleotides
HGI48_RS09725: HGI48_RS09725 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 2,100,415 to 2,101,152
_RS09725
HGI48_RS09730: HGI48_RS09730 - imidazole glycerol phosphate synthase subunit HisH, at 2,101,159 to 2,101,749
_RS09730
HGI48_RS09735: HGI48_RS09735 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 2,101,749 to 2,102,816
_RS09735
HGI48_RS09740: HGI48_RS09740 - histidinol-phosphate transaminase, at 2,102,831 to 2,103,901
_RS09740
Position (kb)
2101
2102
2103 Strain fitness (log2 ratio)
-2
-1
0
1 at 2101.232 kb on + strand, within HGI48_RS09730 at 2101.438 kb on + strand, within HGI48_RS09730 at 2101.697 kb on + strand at 2101.698 kb on - strand at 2101.975 kb on + strand, within HGI48_RS09735 at 2101.975 kb on + strand, within HGI48_RS09735 at 2101.975 kb on + strand at 2102.041 kb on - strand, within HGI48_RS09735 at 2102.357 kb on + strand, within HGI48_RS09735 at 2102.357 kb on + strand, within HGI48_RS09735 at 2102.440 kb on + strand at 2103.280 kb on + strand, within HGI48_RS09740 at 2103.351 kb on + strand, within HGI48_RS09740 at 2103.351 kb on + strand, within HGI48_RS09740 at 2103.459 kb on + strand, within HGI48_RS09740 at 2103.460 kb on - strand, within HGI48_RS09740
Per-strain Table
Position Strand Gene LocusTag Fraction Norland remove 2,101,232 + HGI48_RS09730 0.12 -0.6 2,101,438 + HGI48_RS09730 0.47 -1.1 2,101,697 + -1.1 2,101,698 - -1.0 2,101,975 + HGI48_RS09735 0.21 -0.7 2,101,975 + HGI48_RS09735 0.21 -0.7 2,101,975 + -1.3 2,102,041 - HGI48_RS09735 0.27 -0.5 2,102,357 + HGI48_RS09735 0.57 +0.3 2,102,357 + HGI48_RS09735 0.57 -0.3 2,102,440 + +0.3 2,103,280 + HGI48_RS09740 0.42 -0.1 2,103,351 + HGI48_RS09740 0.49 -0.5 2,103,351 + HGI48_RS09740 0.49 -2.0 2,103,459 + HGI48_RS09740 0.59 -2.3 2,103,460 - HGI48_RS09740 0.59 -0.9
Or see this region's nucleotide sequence