Experiment: Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS05515 and HGI48_RS05520 are separated by 178 nucleotides HGI48_RS05520 and HGI48_RS05525 are separated by 161 nucleotides HGI48_RS05525 and HGI48_RS05530 are separated by 96 nucleotides
HGI48_RS05515: HGI48_RS05515 - peroxiredoxin C, at 1,161,805 to 1,162,407
_RS05515
HGI48_RS05520: HGI48_RS05520 - DUF479 domain-containing protein, at 1,162,586 to 1,163,191
_RS05520
HGI48_RS05525: HGI48_RS05525 - tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA, at 1,163,353 to 1,164,420
_RS05525
HGI48_RS05530: HGI48_RS05530 - tRNA guanosine(34) transglycosylase Tgt, at 1,164,517 to 1,165,671
_RS05530
Position (kb)
1163
1164
1165 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1162.447 kb on - strand at 1162.458 kb on + strand at 1162.459 kb on - strand at 1162.462 kb on + strand at 1162.463 kb on - strand at 1162.463 kb on - strand at 1162.464 kb on + strand at 1162.464 kb on + strand at 1162.464 kb on + strand at 1162.465 kb on - strand at 1162.465 kb on - strand at 1162.465 kb on - strand at 1162.469 kb on - strand at 1162.512 kb on + strand at 1162.513 kb on - strand at 1162.539 kb on - strand at 1162.642 kb on + strand at 1162.643 kb on - strand at 1162.643 kb on - strand at 1162.643 kb on - strand at 1162.643 kb on - strand at 1162.651 kb on - strand, within HGI48_RS05520 at 1162.651 kb on - strand, within HGI48_RS05520 at 1162.683 kb on + strand, within HGI48_RS05520 at 1162.683 kb on + strand at 1162.815 kb on + strand, within HGI48_RS05520 at 1162.816 kb on - strand, within HGI48_RS05520 at 1162.818 kb on + strand, within HGI48_RS05520 at 1162.818 kb on + strand, within HGI48_RS05520 at 1162.818 kb on + strand, within HGI48_RS05520 at 1162.819 kb on - strand, within HGI48_RS05520 at 1162.819 kb on - strand, within HGI48_RS05520 at 1162.819 kb on - strand, within HGI48_RS05520 at 1162.822 kb on + strand at 1162.822 kb on + strand, within HGI48_RS05520 at 1162.822 kb on + strand, within HGI48_RS05520 at 1162.823 kb on - strand at 1162.823 kb on - strand, within HGI48_RS05520 at 1162.823 kb on - strand, within HGI48_RS05520 at 1162.969 kb on + strand, within HGI48_RS05520 at 1162.969 kb on + strand, within HGI48_RS05520 at 1162.970 kb on - strand, within HGI48_RS05520 at 1163.092 kb on - strand, within HGI48_RS05520 at 1163.228 kb on - strand at 1163.228 kb on - strand at 1163.228 kb on - strand at 1163.243 kb on + strand at 1163.243 kb on + strand at 1163.243 kb on + strand at 1163.243 kb on + strand at 1163.243 kb on + strand at 1163.243 kb on + strand at 1163.243 kb on + strand at 1163.244 kb on - strand at 1163.244 kb on - strand at 1163.244 kb on - strand at 1163.244 kb on - strand at 1163.244 kb on - strand at 1163.244 kb on - strand at 1163.244 kb on - strand at 1163.244 kb on - strand at 1163.246 kb on - strand at 1163.272 kb on + strand at 1163.273 kb on - strand at 1163.301 kb on + strand at 1163.306 kb on + strand at 1163.307 kb on - strand at 1163.307 kb on - strand at 1163.414 kb on + strand at 1163.415 kb on - strand at 1163.415 kb on - strand at 1163.496 kb on + strand, within HGI48_RS05525 at 1163.496 kb on + strand, within HGI48_RS05525 at 1163.497 kb on - strand, within HGI48_RS05525 at 1163.598 kb on - strand at 1163.790 kb on + strand, within HGI48_RS05525 at 1163.791 kb on - strand, within HGI48_RS05525 at 1163.791 kb on - strand, within HGI48_RS05525 at 1163.806 kb on + strand, within HGI48_RS05525 at 1163.807 kb on - strand, within HGI48_RS05525 at 1163.807 kb on - strand, within HGI48_RS05525 at 1163.807 kb on - strand, within HGI48_RS05525 at 1163.807 kb on - strand, within HGI48_RS05525 at 1163.857 kb on + strand, within HGI48_RS05525 at 1163.983 kb on - strand, within HGI48_RS05525 at 1164.038 kb on - strand, within HGI48_RS05525 at 1164.196 kb on - strand, within HGI48_RS05525 at 1164.202 kb on - strand, within HGI48_RS05525 at 1164.202 kb on - strand, within HGI48_RS05525 at 1164.220 kb on + strand, within HGI48_RS05525 at 1164.277 kb on - strand, within HGI48_RS05525 at 1164.277 kb on - strand, within HGI48_RS05525 at 1164.277 kb on - strand, within HGI48_RS05525 at 1164.309 kb on + strand, within HGI48_RS05525 at 1164.310 kb on - strand, within HGI48_RS05525 at 1164.310 kb on - strand, within HGI48_RS05525 at 1164.310 kb on - strand, within HGI48_RS05525 at 1164.310 kb on - strand, within HGI48_RS05525 at 1164.473 kb on - strand at 1164.473 kb on - strand at 1164.474 kb on + strand at 1164.474 kb on + strand at 1164.474 kb on + strand at 1164.475 kb on - strand at 1164.475 kb on - strand at 1164.475 kb on - strand at 1164.475 kb on - strand at 1164.475 kb on - strand at 1164.604 kb on - strand at 1164.619 kb on + strand at 1164.753 kb on + strand, within HGI48_RS05530 at 1164.805 kb on - strand, within HGI48_RS05530 at 1164.843 kb on - strand, within HGI48_RS05530 at 1164.873 kb on + strand, within HGI48_RS05530 at 1164.958 kb on + strand, within HGI48_RS05530 at 1165.043 kb on + strand, within HGI48_RS05530 at 1165.043 kb on + strand, within HGI48_RS05530 at 1165.043 kb on + strand, within HGI48_RS05530 at 1165.044 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Norland remove 1,162,447 - +0.9 1,162,458 + +0.8 1,162,459 - +0.2 1,162,462 + +0.2 1,162,463 - -0.2 1,162,463 - +0.7 1,162,464 + +0.5 1,162,464 + +0.1 1,162,464 + +0.1 1,162,465 - +0.7 1,162,465 - -0.3 1,162,465 - +0.4 1,162,469 - +0.8 1,162,512 + +0.7 1,162,513 - +0.7 1,162,539 - -0.3 1,162,642 + -0.6 1,162,643 - +0.7 1,162,643 - -0.0 1,162,643 - +0.4 1,162,643 - +0.3 1,162,651 - HGI48_RS05520 0.11 -1.0 1,162,651 - HGI48_RS05520 0.11 -0.5 1,162,683 + HGI48_RS05520 0.16 -0.3 1,162,683 + -0.9 1,162,815 + HGI48_RS05520 0.38 -0.7 1,162,816 - HGI48_RS05520 0.38 +0.2 1,162,818 + HGI48_RS05520 0.38 -1.3 1,162,818 + HGI48_RS05520 0.38 +0.3 1,162,818 + HGI48_RS05520 0.38 -1.2 1,162,819 - HGI48_RS05520 0.38 -1.7 1,162,819 - HGI48_RS05520 0.38 -0.6 1,162,819 - HGI48_RS05520 0.38 -1.1 1,162,822 + -0.9 1,162,822 + HGI48_RS05520 0.39 -1.8 1,162,822 + HGI48_RS05520 0.39 +0.5 1,162,823 - +1.4 1,162,823 - HGI48_RS05520 0.39 +0.8 1,162,823 - HGI48_RS05520 0.39 +0.5 1,162,969 + HGI48_RS05520 0.63 -0.6 1,162,969 + HGI48_RS05520 0.63 +1.0 1,162,970 - HGI48_RS05520 0.63 -1.3 1,163,092 - HGI48_RS05520 0.83 +0.5 1,163,228 - -0.9 1,163,228 - +2.7 1,163,228 - +0.3 1,163,243 + -2.5 1,163,243 + +0.9 1,163,243 + +1.1 1,163,243 + +0.7 1,163,243 + -0.9 1,163,243 + +0.5 1,163,243 + -1.7 1,163,244 - -0.3 1,163,244 - -0.5 1,163,244 - +0.3 1,163,244 - +0.9 1,163,244 - +0.5 1,163,244 - -1.8 1,163,244 - +1.4 1,163,244 - -2.2 1,163,246 - -1.3 1,163,272 + -1.7 1,163,273 - +0.4 1,163,301 + -0.0 1,163,306 + -0.2 1,163,307 - +0.2 1,163,307 - -0.5 1,163,414 + -2.7 1,163,415 - +0.7 1,163,415 - -0.6 1,163,496 + HGI48_RS05525 0.13 -2.2 1,163,496 + HGI48_RS05525 0.13 +0.7 1,163,497 - HGI48_RS05525 0.13 -0.9 1,163,598 - -0.9 1,163,790 + HGI48_RS05525 0.41 -0.9 1,163,791 - HGI48_RS05525 0.41 -0.3 1,163,791 - HGI48_RS05525 0.41 -1.3 1,163,806 + HGI48_RS05525 0.42 -0.1 1,163,807 - HGI48_RS05525 0.43 -0.2 1,163,807 - HGI48_RS05525 0.43 -1.9 1,163,807 - HGI48_RS05525 0.43 -0.6 1,163,807 - HGI48_RS05525 0.43 -1.8 1,163,857 + HGI48_RS05525 0.47 -1.3 1,163,983 - HGI48_RS05525 0.59 -0.4 1,164,038 - HGI48_RS05525 0.64 -0.2 1,164,196 - HGI48_RS05525 0.79 -1.3 1,164,202 - HGI48_RS05525 0.79 -0.9 1,164,202 - HGI48_RS05525 0.79 -1.1 1,164,220 + HGI48_RS05525 0.81 -1.9 1,164,277 - HGI48_RS05525 0.87 +0.9 1,164,277 - HGI48_RS05525 0.87 +0.2 1,164,277 - HGI48_RS05525 0.87 +1.2 1,164,309 + HGI48_RS05525 0.90 -0.6 1,164,310 - HGI48_RS05525 0.90 -1.2 1,164,310 - HGI48_RS05525 0.90 -1.8 1,164,310 - HGI48_RS05525 0.90 -0.1 1,164,310 - HGI48_RS05525 0.90 +0.5 1,164,473 - -2.3 1,164,473 - -0.7 1,164,474 + -0.8 1,164,474 + -2.3 1,164,474 + +0.1 1,164,475 - +1.2 1,164,475 - -0.2 1,164,475 - +0.7 1,164,475 - +0.2 1,164,475 - -0.2 1,164,604 - -0.9 1,164,619 + +0.3 1,164,753 + HGI48_RS05530 0.20 +0.7 1,164,805 - HGI48_RS05530 0.25 -0.7 1,164,843 - HGI48_RS05530 0.28 -0.1 1,164,873 + HGI48_RS05530 0.31 -0.7 1,164,958 + HGI48_RS05530 0.38 -0.3 1,165,043 + HGI48_RS05530 0.46 -2.9 1,165,043 + HGI48_RS05530 0.46 +0.3 1,165,043 + HGI48_RS05530 0.46 -0.1 1,165,044 - -0.3
Or see this region's nucleotide sequence