Experiment: Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS00010 and HGI48_RS00015 are separated by 165 nucleotides HGI48_RS00015 and HGI48_RS00020 are separated by 17 nucleotides
HGI48_RS00010: HGI48_RS00010 - DNA polymerase III subunit beta, at 1,325 to 2,425
_RS00010
HGI48_RS00015: HGI48_RS00015 - DNA replication/repair protein RecF, at 2,591 to 3,676
_RS00015
HGI48_RS00020: HGI48_RS00020 - DNA topoisomerase (ATP-hydrolyzing) subunit B, at 3,694 to 6,105
_RS00020
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1 at 2.423 kb on + strand at 2.424 kb on - strand at 2.424 kb on - strand at 2.424 kb on - strand at 2.517 kb on + strand at 2.537 kb on + strand at 2.538 kb on - strand at 2.538 kb on - strand at 2.540 kb on + strand at 2.612 kb on - strand at 2.629 kb on + strand at 2.720 kb on + strand, within HGI48_RS00015 at 2.721 kb on - strand, within HGI48_RS00015 at 2.721 kb on - strand, within HGI48_RS00015 at 2.721 kb on - strand at 2.721 kb on - strand at 2.721 kb on - strand, within HGI48_RS00015 at 2.753 kb on - strand, within HGI48_RS00015 at 2.753 kb on - strand, within HGI48_RS00015 at 2.770 kb on - strand, within HGI48_RS00015 at 2.981 kb on + strand, within HGI48_RS00015 at 3.102 kb on - strand, within HGI48_RS00015 at 3.173 kb on + strand, within HGI48_RS00015 at 3.173 kb on + strand, within HGI48_RS00015 at 3.174 kb on - strand, within HGI48_RS00015 at 3.486 kb on - strand, within HGI48_RS00015 at 3.486 kb on - strand, within HGI48_RS00015 at 3.654 kb on + strand at 3.654 kb on + strand at 3.674 kb on + strand at 3.674 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Norland remove 2,423 + -1.9 2,424 - +0.9 2,424 - +1.2 2,424 - -0.3 2,517 + -0.9 2,537 + -1.8 2,538 - -0.7 2,538 - -0.3 2,540 + +1.7 2,612 - -1.7 2,629 + +0.9 2,720 + HGI48_RS00015 0.12 -1.7 2,721 - HGI48_RS00015 0.12 -1.4 2,721 - HGI48_RS00015 0.12 -0.4 2,721 - -0.7 2,721 - -0.1 2,721 - HGI48_RS00015 0.12 -2.5 2,753 - HGI48_RS00015 0.15 +0.2 2,753 - HGI48_RS00015 0.15 -2.4 2,770 - HGI48_RS00015 0.16 -1.7 2,981 + HGI48_RS00015 0.36 +0.2 3,102 - HGI48_RS00015 0.47 -0.5 3,173 + HGI48_RS00015 0.54 -0.1 3,173 + HGI48_RS00015 0.54 -0.3 3,174 - HGI48_RS00015 0.54 +0.6 3,486 - HGI48_RS00015 0.82 +0.5 3,486 - HGI48_RS00015 0.82 -0.1 3,654 + +0.9 3,654 + -1.1 3,674 + +0.2 3,674 + -0.1
Or see this region's nucleotide sequence