Experiment: Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS14195 and HGI48_RS14200 are separated by 63 nucleotides HGI48_RS14200 and HGI48_RS14205 overlap by 8 nucleotides HGI48_RS14205 and HGI48_RS14210 overlap by 11 nucleotides HGI48_RS14210 and HGI48_RS14215 overlap by 1 nucleotides
HGI48_RS14195: HGI48_RS14195 - hydrogenase nickel incorporation protein HypB, at 3,185,424 to 3,186,572
_RS14195
HGI48_RS14200: HGI48_RS14200 - hydrogenase maturation nickel metallochaperone HypA, at 3,186,636 to 3,186,977
_RS14200
HGI48_RS14205: HGI48_RS14205 - hydrogenase-2 assembly chaperone, at 3,186,970 to 3,187,536
_RS14205
HGI48_RS14210: HGI48_RS14210 - HyaD/HybD family hydrogenase maturation endopeptidase, at 3,187,526 to 3,188,026
_RS14210
HGI48_RS14215: HGI48_RS14215 - hydrogenase 2 large subunit, at 3,188,026 to 3,189,732
_RS14215
Position (kb)
3187
3188
3189 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3186.531 kb on + strand at 3186.532 kb on - strand at 3186.566 kb on + strand at 3186.567 kb on - strand at 3186.567 kb on - strand at 3186.577 kb on - strand at 3186.577 kb on - strand at 3186.693 kb on - strand, within HGI48_RS14200 at 3186.936 kb on + strand, within HGI48_RS14200 at 3186.936 kb on + strand, within HGI48_RS14200 at 3186.937 kb on - strand, within HGI48_RS14200 at 3186.956 kb on - strand at 3187.148 kb on + strand, within HGI48_RS14205 at 3187.148 kb on + strand, within HGI48_RS14205 at 3187.264 kb on + strand at 3187.264 kb on + strand, within HGI48_RS14205 at 3187.265 kb on - strand, within HGI48_RS14205 at 3187.265 kb on - strand, within HGI48_RS14205 at 3187.267 kb on + strand, within HGI48_RS14205 at 3187.267 kb on + strand at 3187.267 kb on + strand, within HGI48_RS14205 at 3187.267 kb on + strand at 3187.267 kb on + strand, within HGI48_RS14205 at 3187.267 kb on + strand, within HGI48_RS14205 at 3187.267 kb on + strand, within HGI48_RS14205 at 3187.267 kb on + strand, within HGI48_RS14205 at 3187.268 kb on - strand, within HGI48_RS14205 at 3187.268 kb on - strand at 3187.268 kb on - strand, within HGI48_RS14205 at 3187.270 kb on + strand, within HGI48_RS14205 at 3187.270 kb on + strand, within HGI48_RS14205 at 3187.270 kb on + strand, within HGI48_RS14205 at 3187.271 kb on - strand, within HGI48_RS14205 at 3187.271 kb on - strand, within HGI48_RS14205 at 3187.326 kb on + strand, within HGI48_RS14205 at 3187.326 kb on + strand, within HGI48_RS14205 at 3187.327 kb on - strand, within HGI48_RS14205 at 3187.380 kb on + strand, within HGI48_RS14205 at 3187.381 kb on - strand, within HGI48_RS14205 at 3187.491 kb on + strand at 3187.492 kb on - strand at 3187.536 kb on + strand at 3187.536 kb on + strand at 3187.536 kb on + strand at 3187.536 kb on + strand at 3187.536 kb on + strand at 3187.536 kb on + strand at 3187.536 kb on + strand at 3187.536 kb on + strand at 3187.537 kb on - strand at 3187.537 kb on - strand at 3187.537 kb on - strand at 3187.537 kb on - strand at 3187.537 kb on - strand at 3187.537 kb on - strand at 3187.537 kb on - strand at 3187.537 kb on - strand at 3187.537 kb on - strand at 3187.537 kb on - strand at 3187.537 kb on - strand at 3187.598 kb on - strand, within HGI48_RS14210 at 3187.598 kb on - strand, within HGI48_RS14210 at 3187.598 kb on - strand, within HGI48_RS14210 at 3188.018 kb on + strand at 3188.018 kb on + strand at 3188.018 kb on + strand at 3188.018 kb on + strand at 3188.018 kb on + strand at 3188.018 kb on + strand at 3188.019 kb on - strand at 3188.019 kb on - strand at 3188.194 kb on - strand at 3188.246 kb on + strand, within HGI48_RS14215 at 3188.246 kb on + strand at 3188.246 kb on + strand, within HGI48_RS14215 at 3188.247 kb on - strand at 3188.247 kb on - strand, within HGI48_RS14215 at 3188.268 kb on + strand, within HGI48_RS14215 at 3188.432 kb on - strand, within HGI48_RS14215 at 3188.448 kb on + strand, within HGI48_RS14215 at 3188.559 kb on + strand, within HGI48_RS14215 at 3188.559 kb on + strand, within HGI48_RS14215 at 3188.559 kb on + strand, within HGI48_RS14215 at 3188.559 kb on + strand, within HGI48_RS14215 at 3188.560 kb on - strand, within HGI48_RS14215 at 3188.560 kb on - strand, within HGI48_RS14215 at 3188.621 kb on + strand, within HGI48_RS14215 at 3188.648 kb on + strand, within HGI48_RS14215 at 3188.648 kb on + strand, within HGI48_RS14215 at 3188.648 kb on + strand, within HGI48_RS14215 at 3188.649 kb on - strand, within HGI48_RS14215 at 3188.649 kb on - strand, within HGI48_RS14215 at 3188.675 kb on + strand, within HGI48_RS14215 at 3188.675 kb on + strand, within HGI48_RS14215 at 3188.772 kb on + strand, within HGI48_RS14215 at 3188.772 kb on + strand, within HGI48_RS14215 at 3188.773 kb on - strand, within HGI48_RS14215 at 3188.773 kb on - strand at 3188.888 kb on + strand at 3188.888 kb on + strand, within HGI48_RS14215 at 3188.888 kb on + strand, within HGI48_RS14215 at 3188.889 kb on - strand, within HGI48_RS14215 at 3188.960 kb on + strand, within HGI48_RS14215 at 3188.960 kb on + strand at 3188.973 kb on + strand, within HGI48_RS14215 at 3189.008 kb on + strand, within HGI48_RS14215 at 3189.008 kb on + strand at 3189.009 kb on - strand, within HGI48_RS14215 at 3189.009 kb on - strand, within HGI48_RS14215 at 3189.009 kb on - strand, within HGI48_RS14215 at 3189.009 kb on - strand, within HGI48_RS14215
Per-strain Table
Position Strand Gene LocusTag Fraction Norland remove 3,186,531 + +0.5 3,186,532 - +0.2 3,186,566 + -0.6 3,186,567 - +0.5 3,186,567 - +0.5 3,186,577 - -2.3 3,186,577 - -0.8 3,186,693 - HGI48_RS14200 0.17 +1.1 3,186,936 + HGI48_RS14200 0.88 +0.1 3,186,936 + HGI48_RS14200 0.88 -0.8 3,186,937 - HGI48_RS14200 0.88 -0.7 3,186,956 - -0.0 3,187,148 + HGI48_RS14205 0.31 +0.7 3,187,148 + HGI48_RS14205 0.31 -0.1 3,187,264 + -1.0 3,187,264 + HGI48_RS14205 0.52 +0.5 3,187,265 - HGI48_RS14205 0.52 +0.1 3,187,265 - HGI48_RS14205 0.52 -1.6 3,187,267 + HGI48_RS14205 0.52 -0.3 3,187,267 + +1.5 3,187,267 + HGI48_RS14205 0.52 -0.0 3,187,267 + +0.5 3,187,267 + HGI48_RS14205 0.52 -0.3 3,187,267 + HGI48_RS14205 0.52 -1.5 3,187,267 + HGI48_RS14205 0.52 +0.5 3,187,267 + HGI48_RS14205 0.52 +0.5 3,187,268 - HGI48_RS14205 0.53 -0.3 3,187,268 - +0.5 3,187,268 - HGI48_RS14205 0.53 -1.6 3,187,270 + HGI48_RS14205 0.53 -0.3 3,187,270 + HGI48_RS14205 0.53 -1.5 3,187,270 + HGI48_RS14205 0.53 -1.0 3,187,271 - HGI48_RS14205 0.53 -0.8 3,187,271 - HGI48_RS14205 0.53 -1.8 3,187,326 + HGI48_RS14205 0.63 +0.8 3,187,326 + HGI48_RS14205 0.63 +1.1 3,187,327 - HGI48_RS14205 0.63 +0.1 3,187,380 + HGI48_RS14205 0.72 -1.8 3,187,381 - HGI48_RS14205 0.72 +0.0 3,187,491 + -0.8 3,187,492 - -0.2 3,187,536 + -2.3 3,187,536 + -0.8 3,187,536 + -0.5 3,187,536 + +0.3 3,187,536 + -1.5 3,187,536 + +0.2 3,187,536 + -0.3 3,187,536 + -2.9 3,187,537 - +0.5 3,187,537 - +1.8 3,187,537 - +0.5 3,187,537 - +0.7 3,187,537 - -0.1 3,187,537 - -0.5 3,187,537 - -2.3 3,187,537 - -1.6 3,187,537 - +0.7 3,187,537 - +0.5 3,187,537 - -1.5 3,187,598 - HGI48_RS14210 0.14 -0.8 3,187,598 - HGI48_RS14210 0.14 -1.7 3,187,598 - HGI48_RS14210 0.14 -1.1 3,188,018 + +1.0 3,188,018 + +0.9 3,188,018 + +0.3 3,188,018 + -0.9 3,188,018 + +0.1 3,188,018 + +0.9 3,188,019 - +0.3 3,188,019 - -0.8 3,188,194 - -0.0 3,188,246 + HGI48_RS14215 0.13 -0.3 3,188,246 + +1.5 3,188,246 + HGI48_RS14215 0.13 -0.3 3,188,247 - +1.3 3,188,247 - HGI48_RS14215 0.13 -0.8 3,188,268 + HGI48_RS14215 0.14 +0.1 3,188,432 - HGI48_RS14215 0.24 +0.0 3,188,448 + HGI48_RS14215 0.25 +0.4 3,188,559 + HGI48_RS14215 0.31 -0.5 3,188,559 + HGI48_RS14215 0.31 -0.5 3,188,559 + HGI48_RS14215 0.31 -2.3 3,188,559 + HGI48_RS14215 0.31 -1.3 3,188,560 - HGI48_RS14215 0.31 -0.8 3,188,560 - HGI48_RS14215 0.31 -1.0 3,188,621 + HGI48_RS14215 0.35 +1.5 3,188,648 + HGI48_RS14215 0.36 +0.5 3,188,648 + HGI48_RS14215 0.36 -0.2 3,188,648 + HGI48_RS14215 0.36 +0.0 3,188,649 - HGI48_RS14215 0.36 +0.1 3,188,649 - HGI48_RS14215 0.36 -0.3 3,188,675 + HGI48_RS14215 0.38 -0.2 3,188,675 + HGI48_RS14215 0.38 +0.3 3,188,772 + HGI48_RS14215 0.44 -0.1 3,188,772 + HGI48_RS14215 0.44 -2.8 3,188,773 - HGI48_RS14215 0.44 +0.7 3,188,773 - +1.0 3,188,888 + -1.0 3,188,888 + HGI48_RS14215 0.50 +0.1 3,188,888 + HGI48_RS14215 0.50 +0.9 3,188,889 - HGI48_RS14215 0.51 -1.8 3,188,960 + HGI48_RS14215 0.55 +0.9 3,188,960 + +0.5 3,188,973 + HGI48_RS14215 0.55 +0.7 3,189,008 + HGI48_RS14215 0.58 -1.5 3,189,008 + +1.5 3,189,009 - HGI48_RS14215 0.58 -1.5 3,189,009 - HGI48_RS14215 0.58 -0.3 3,189,009 - HGI48_RS14215 0.58 -1.8 3,189,009 - HGI48_RS14215 0.58 +0.2
Or see this region's nucleotide sequence