Experiment: Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01020 and HGI48_RS01025 overlap by 4 nucleotides HGI48_RS01025 and HGI48_RS01030 are separated by 26 nucleotides HGI48_RS01030 and HGI48_RS01035 are separated by 683 nucleotides HGI48_RS01035 and HGI48_RS01040 are separated by 16 nucleotides HGI48_RS01040 and HGI48_RS01045 are separated by 135 nucleotides
HGI48_RS01020: HGI48_RS01020 - UDP-N-acetylmuramate dehydrogenase, at 232,181 to 233,218
_RS01020
HGI48_RS01025: HGI48_RS01025 - bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA, at 233,215 to 234,174
_RS01025
HGI48_RS01030: HGI48_RS01030 - type I pantothenate kinase, at 234,201 to 235,151
_RS01030
HGI48_RS01035: HGI48_RS01035 - tRNA-Thr, at 235,835 to 235,910
_RS01035
HGI48_RS01040: HGI48_RS01040 - tRNA-Tyr, at 235,927 to 236,011
_RS01040
HGI48_RS01045: HGI48_RS01045 - tRNA-Gly, at 236,147 to 236,221
_RS01045
Position (kb)
234
235
236 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 233.224 kb on + strand at 233.226 kb on + strand at 234.176 kb on - strand at 235.152 kb on - strand at 235.158 kb on - strand at 235.158 kb on - strand at 235.158 kb on - strand at 235.328 kb on + strand at 235.350 kb on - strand at 235.390 kb on - strand at 235.417 kb on + strand at 235.417 kb on + strand at 235.417 kb on + strand at 235.417 kb on + strand at 235.420 kb on + strand at 235.421 kb on - strand at 235.421 kb on - strand at 235.452 kb on + strand at 235.452 kb on + strand at 235.452 kb on + strand at 235.452 kb on + strand at 235.453 kb on - strand at 235.453 kb on - strand at 235.453 kb on - strand at 235.461 kb on + strand at 235.462 kb on - strand at 235.462 kb on - strand at 235.462 kb on - strand at 235.504 kb on - strand at 235.525 kb on + strand at 235.526 kb on - strand at 235.546 kb on - strand at 235.618 kb on + strand at 235.619 kb on - strand at 235.668 kb on + strand at 235.668 kb on + strand at 235.668 kb on + strand at 235.685 kb on + strand at 235.686 kb on - strand at 235.705 kb on - strand at 235.705 kb on - strand at 236.035 kb on + strand at 236.035 kb on + strand at 236.035 kb on + strand at 236.047 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Norland remove 233,224 + -2.2 233,226 + +1.3 234,176 - -1.6 235,152 - +1.0 235,158 - -0.5 235,158 - -2.0 235,158 - +0.5 235,328 + +1.1 235,350 - -0.2 235,390 - -1.3 235,417 + -1.7 235,417 + -1.2 235,417 + -0.0 235,417 + +1.4 235,420 + -2.2 235,421 - +0.5 235,421 - -1.0 235,452 + -0.9 235,452 + -0.0 235,452 + -0.6 235,452 + +0.2 235,453 - +0.7 235,453 - +0.2 235,453 - +0.6 235,461 + +1.5 235,462 - +1.7 235,462 - -1.0 235,462 - -0.3 235,504 - +0.4 235,525 + +2.1 235,526 - +0.7 235,546 - -1.8 235,618 + -1.0 235,619 - -0.2 235,668 + -1.0 235,668 + -1.2 235,668 + +1.6 235,685 + +0.3 235,686 - -0.6 235,705 - +1.3 235,705 - -1.0 236,035 + -1.0 236,035 + -0.8 236,035 + -0.0 236,047 + -0.8
Or see this region's nucleotide sequence