Strain Fitness in Dickeya dianthicola 67-19 around HGI48_RS21435

Experiment: Atlantic

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntHGI48_RS21425 and HGI48_RS21430 are separated by 29 nucleotidesHGI48_RS21430 and HGI48_RS21435 are separated by 145 nucleotidesHGI48_RS21435 and HGI48_RS21440 are separated by 221 nucleotides HGI48_RS21425: HGI48_RS21425 - F0F1 ATP synthase subunit beta, at 4,781,529 to 4,782,911 _RS21425 HGI48_RS21430: HGI48_RS21430 - F0F1 ATP synthase subunit epsilon, at 4,782,941 to 4,783,354 _RS21430 HGI48_RS21435: HGI48_RS21435 - right-handed parallel beta-helix repeat-containing protein, at 4,783,500 to 4,785,704 _RS21435 HGI48_RS21440: HGI48_RS21440 - bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU, at 4,785,926 to 4,787,296 _RS21440 Position (kb) 4783 4784 4785 4786Strain fitness (log2 ratio) -2 -1 0 1 2at 4783.258 kb on + strand, within HGI48_RS21430at 4783.258 kb on + strand, within HGI48_RS21430at 4783.277 kb on + strand, within HGI48_RS21430at 4783.277 kb on + strand, within HGI48_RS21430at 4783.277 kb on + strand, within HGI48_RS21430at 4783.278 kb on - strand, within HGI48_RS21430at 4783.278 kb on - strand, within HGI48_RS21430at 4783.352 kb on + strandat 4783.352 kb on + strandat 4783.352 kb on + strandat 4783.352 kb on + strandat 4783.353 kb on - strandat 4783.367 kb on + strandat 4783.367 kb on + strandat 4783.368 kb on - strandat 4783.368 kb on - strandat 4783.385 kb on + strandat 4783.385 kb on + strandat 4783.386 kb on - strandat 4783.386 kb on - strandat 4783.386 kb on - strandat 4783.386 kb on - strandat 4783.492 kb on + strandat 4783.492 kb on + strandat 4783.493 kb on - strandat 4783.493 kb on - strandat 4783.536 kb on - strandat 4783.536 kb on - strandat 4783.582 kb on + strandat 4783.583 kb on - strandat 4783.642 kb on + strandat 4783.642 kb on + strandat 4783.642 kb on + strandat 4783.642 kb on + strandat 4783.642 kb on + strandat 4783.643 kb on - strandat 4783.643 kb on - strandat 4783.643 kb on - strandat 4783.643 kb on - strandat 4783.643 kb on - strandat 4783.643 kb on - strandat 4783.681 kb on + strandat 4783.681 kb on + strandat 4783.682 kb on - strandat 4783.682 kb on - strandat 4783.682 kb on - strandat 4783.682 kb on - strandat 4783.682 kb on - strandat 4783.698 kb on - strandat 4783.724 kb on + strand, within HGI48_RS21435at 4783.724 kb on + strand, within HGI48_RS21435at 4783.725 kb on - strand, within HGI48_RS21435at 4783.745 kb on - strand, within HGI48_RS21435at 4783.745 kb on - strand, within HGI48_RS21435at 4783.748 kb on - strand, within HGI48_RS21435at 4783.766 kb on + strand, within HGI48_RS21435at 4783.767 kb on - strand, within HGI48_RS21435at 4783.871 kb on + strand, within HGI48_RS21435at 4783.871 kb on + strand, within HGI48_RS21435at 4783.871 kb on + strand, within HGI48_RS21435at 4783.872 kb on - strand, within HGI48_RS21435at 4783.916 kb on + strand, within HGI48_RS21435at 4784.145 kb on - strand, within HGI48_RS21435at 4784.196 kb on - strand, within HGI48_RS21435at 4784.300 kb on - strand, within HGI48_RS21435at 4784.360 kb on + strand, within HGI48_RS21435at 4784.360 kb on + strand, within HGI48_RS21435at 4784.360 kb on + strand, within HGI48_RS21435at 4784.361 kb on - strand, within HGI48_RS21435at 4784.361 kb on - strand, within HGI48_RS21435at 4784.397 kb on + strand, within HGI48_RS21435at 4784.398 kb on - strand, within HGI48_RS21435at 4784.398 kb on - strand, within HGI48_RS21435at 4784.410 kb on + strand, within HGI48_RS21435at 4784.411 kb on - strand, within HGI48_RS21435at 4784.527 kb on + strand, within HGI48_RS21435at 4784.527 kb on + strandat 4784.527 kb on + strand, within HGI48_RS21435at 4784.527 kb on + strandat 4784.527 kb on + strand, within HGI48_RS21435at 4784.527 kb on + strand, within HGI48_RS21435at 4784.527 kb on + strandat 4784.527 kb on + strand, within HGI48_RS21435at 4784.527 kb on + strand, within HGI48_RS21435at 4784.527 kb on + strand, within HGI48_RS21435at 4784.528 kb on - strand, within HGI48_RS21435at 4784.528 kb on - strand, within HGI48_RS21435at 4784.528 kb on - strand, within HGI48_RS21435at 4784.614 kb on + strand, within HGI48_RS21435at 4784.614 kb on + strand, within HGI48_RS21435at 4784.615 kb on - strand, within HGI48_RS21435at 4784.615 kb on - strand, within HGI48_RS21435at 4784.615 kb on - strand, within HGI48_RS21435at 4784.657 kb on + strand, within HGI48_RS21435at 4784.657 kb on + strand, within HGI48_RS21435at 4784.657 kb on + strand, within HGI48_RS21435at 4784.669 kb on - strandat 4784.738 kb on + strand, within HGI48_RS21435at 4784.740 kb on + strand, within HGI48_RS21435at 4784.740 kb on + strand, within HGI48_RS21435at 4784.740 kb on + strand, within HGI48_RS21435at 4784.740 kb on + strand, within HGI48_RS21435at 4784.740 kb on + strand, within HGI48_RS21435at 4784.740 kb on + strand, within HGI48_RS21435at 4784.740 kb on + strand, within HGI48_RS21435at 4784.741 kb on - strand, within HGI48_RS21435at 4784.741 kb on - strand, within HGI48_RS21435at 4784.741 kb on - strand, within HGI48_RS21435at 4784.741 kb on - strand, within HGI48_RS21435at 4784.741 kb on - strand, within HGI48_RS21435at 4784.764 kb on + strandat 4784.796 kb on + strand, within HGI48_RS21435at 4784.796 kb on + strand, within HGI48_RS21435at 4784.797 kb on - strand, within HGI48_RS21435at 4784.797 kb on - strandat 4784.889 kb on + strand, within HGI48_RS21435at 4784.889 kb on + strand, within HGI48_RS21435at 4784.890 kb on - strand, within HGI48_RS21435at 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strandat 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strandat 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strandat 4784.923 kb on + strandat 4784.923 kb on + strandat 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strand, within HGI48_RS21435at 4784.923 kb on + strand, within HGI48_RS21435at 4784.924 kb on - strand, within HGI48_RS21435at 4784.924 kb on - strand, within HGI48_RS21435at 4784.924 kb on - strand, within HGI48_RS21435at 4784.924 kb on - strand, within HGI48_RS21435at 4784.924 kb on - strand, within HGI48_RS21435at 4784.924 kb on - strand, within HGI48_RS21435at 4784.924 kb on - strand, within HGI48_RS21435at 4784.924 kb on - strand, within HGI48_RS21435at 4784.924 kb on - strand, within HGI48_RS21435at 4784.924 kb on - strand, within HGI48_RS21435at 4784.924 kb on - strand, within HGI48_RS21435at 4784.924 kb on - strand, within HGI48_RS21435at 4784.924 kb on - strand, within HGI48_RS21435at 4784.924 kb on - strand, within HGI48_RS21435at 4784.924 kb on - strand, within HGI48_RS21435at 4784.924 kb on - strand, within HGI48_RS21435at 4784.926 kb on + strand, within HGI48_RS21435at 4784.926 kb on + strand, within HGI48_RS21435at 4784.927 kb on - strand, within HGI48_RS21435at 4784.927 kb on - strandat 4784.927 kb on - strand, within HGI48_RS21435at 4784.927 kb on - strand, within HGI48_RS21435at 4784.927 kb on - strand, within HGI48_RS21435at 4784.927 kb on - strand, within HGI48_RS21435at 4785.007 kb on + strand, within HGI48_RS21435at 4785.008 kb on - strand, within HGI48_RS21435at 4785.008 kb on - strand, within HGI48_RS21435at 4785.015 kb on - strand, within HGI48_RS21435at 4785.089 kb on - strand, within HGI48_RS21435at 4785.185 kb on + strand, within HGI48_RS21435at 4785.185 kb on + strand, within HGI48_RS21435at 4785.186 kb on - strand, within HGI48_RS21435at 4785.186 kb on - strand, within HGI48_RS21435at 4785.241 kb on + strand, within HGI48_RS21435at 4785.241 kb on + strand, within HGI48_RS21435at 4785.242 kb on - strandat 4785.242 kb on - strand, within HGI48_RS21435at 4785.404 kb on - strand, within HGI48_RS21435at 4785.428 kb on + strand, within HGI48_RS21435at 4785.428 kb on + strand, within HGI48_RS21435at 4785.428 kb on + strand, within HGI48_RS21435at 4785.429 kb on - strand, within HGI48_RS21435at 4785.429 kb on - strandat 4785.429 kb on - strand, within HGI48_RS21435at 4785.461 kb on + strandat 4785.462 kb on - strand, within HGI48_RS21435at 4785.492 kb on + strandat 4785.492 kb on + strandat 4785.492 kb on + strandat 4785.493 kb on - strandat 4785.493 kb on - strandat 4785.493 kb on - strandat 4785.666 kb on - strandat 4785.666 kb on - strandat 4785.678 kb on + strandat 4785.679 kb on - strandat 4785.679 kb on - strandat 4785.679 kb on - strandat 4785.697 kb on + strandat 4785.697 kb on + strandat 4785.697 kb on + strandat 4785.697 kb on + strandat 4785.697 kb on + strandat 4785.697 kb on + strandat 4785.698 kb on - strandat 4785.698 kb on - strandat 4785.698 kb on - strandat 4785.698 kb on - strandat 4785.698 kb on - strandat 4785.698 kb on - strandat 4785.698 kb on - strandat 4785.698 kb on - strandat 4785.698 kb on - strandat 4785.698 kb on - strandat 4785.719 kb on + strandat 4785.719 kb on + strandat 4785.719 kb on + strandat 4785.719 kb on + strandat 4785.719 kb on + strandat 4785.719 kb on + strandat 4785.720 kb on - strandat 4785.720 kb on - strandat 4785.720 kb on - strandat 4785.720 kb on - strandat 4785.720 kb on - strandat 4785.720 kb on - strandat 4785.720 kb on - strandat 4785.720 kb on - strandat 4785.741 kb on + strandat 4785.742 kb on - strandat 4785.742 kb on - strandat 4785.788 kb on + strandat 4785.824 kb on + strandat 4785.825 kb on - strandat 4785.841 kb on + strandat 4785.842 kb on - strandat 4785.842 kb on - strandat 4785.845 kb on + strandat 4785.845 kb on + strandat 4785.845 kb on + strandat 4785.845 kb on + strandat 4785.845 kb on + strandat 4785.845 kb on + strandat 4785.846 kb on - strandat 4785.846 kb on - strandat 4785.846 kb on - strandat 4785.846 kb on - strandat 4785.846 kb on - strandat 4785.848 kb on + strandat 4785.849 kb on - strandat 4785.849 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Atlantic
remove
4,783,258 + HGI48_RS21430 0.77 -0.2
4,783,258 + HGI48_RS21430 0.77 -1.2
4,783,277 + HGI48_RS21430 0.81 -1.2
4,783,277 + HGI48_RS21430 0.81 -1.2
4,783,277 + HGI48_RS21430 0.81 +0.7
4,783,278 - HGI48_RS21430 0.81 +0.7
4,783,278 - HGI48_RS21430 0.81 -1.0
4,783,352 + -1.9
4,783,352 + -0.4
4,783,352 + +2.1
4,783,352 + +0.1
4,783,353 - -0.5
4,783,367 + +0.4
4,783,367 + -2.6
4,783,368 - -0.8
4,783,368 - +0.8
4,783,385 + -0.4
4,783,385 + -0.7
4,783,386 - -0.9
4,783,386 - -0.4
4,783,386 - +0.8
4,783,386 - -1.2
4,783,492 + -1.7
4,783,492 + +0.1
4,783,493 - +1.3
4,783,493 - +0.7
4,783,536 - -1.2
4,783,536 - +0.8
4,783,582 + +0.1
4,783,583 - -0.9
4,783,642 + +0.3
4,783,642 + -0.7
4,783,642 + -1.0
4,783,642 + -1.4
4,783,642 + -0.9
4,783,643 - +1.7
4,783,643 - -0.2
4,783,643 - -0.1
4,783,643 - -1.3
4,783,643 - +1.1
4,783,643 - -0.1
4,783,681 + +0.6
4,783,681 + +0.1
4,783,682 - +0.0
4,783,682 - +0.3
4,783,682 - -1.7
4,783,682 - +0.9
4,783,682 - -0.4
4,783,698 - -0.9
4,783,724 + HGI48_RS21435 0.10 +0.4
4,783,724 + HGI48_RS21435 0.10 -0.9
4,783,725 - HGI48_RS21435 0.10 -0.7
4,783,745 - HGI48_RS21435 0.11 -0.7
4,783,745 - HGI48_RS21435 0.11 -0.4
4,783,748 - HGI48_RS21435 0.11 -0.9
4,783,766 + HGI48_RS21435 0.12 +0.9
4,783,767 - HGI48_RS21435 0.12 -0.3
4,783,871 + HGI48_RS21435 0.17 -0.3
4,783,871 + HGI48_RS21435 0.17 +0.6
4,783,871 + HGI48_RS21435 0.17 +0.6
4,783,872 - HGI48_RS21435 0.17 -0.7
4,783,916 + HGI48_RS21435 0.19 -0.3
4,784,145 - HGI48_RS21435 0.29 -1.4
4,784,196 - HGI48_RS21435 0.32 -0.0
4,784,300 - HGI48_RS21435 0.36 -0.1
4,784,360 + HGI48_RS21435 0.39 -0.4
4,784,360 + HGI48_RS21435 0.39 -1.2
4,784,360 + HGI48_RS21435 0.39 -0.4
4,784,361 - HGI48_RS21435 0.39 -1.9
4,784,361 - HGI48_RS21435 0.39 +1.9
4,784,397 + HGI48_RS21435 0.41 +0.0
4,784,398 - HGI48_RS21435 0.41 -0.2
4,784,398 - HGI48_RS21435 0.41 -0.4
4,784,410 + HGI48_RS21435 0.41 -0.2
4,784,411 - HGI48_RS21435 0.41 -0.7
4,784,527 + HGI48_RS21435 0.47 +1.1
4,784,527 + +0.1
4,784,527 + HGI48_RS21435 0.47 +1.3
4,784,527 + +0.6
4,784,527 + HGI48_RS21435 0.47 -2.6
4,784,527 + HGI48_RS21435 0.47 -1.0
4,784,527 + -0.4
4,784,527 + HGI48_RS21435 0.47 -0.7
4,784,527 + HGI48_RS21435 0.47 -0.4
4,784,527 + HGI48_RS21435 0.47 +0.1
4,784,528 - HGI48_RS21435 0.47 +0.1
4,784,528 - HGI48_RS21435 0.47 +0.3
4,784,528 - HGI48_RS21435 0.47 -0.2
4,784,614 + HGI48_RS21435 0.51 -1.7
4,784,614 + HGI48_RS21435 0.51 -1.9
4,784,615 - HGI48_RS21435 0.51 -0.7
4,784,615 - HGI48_RS21435 0.51 -1.6
4,784,615 - HGI48_RS21435 0.51 +1.7
4,784,657 + HGI48_RS21435 0.52 -0.4
4,784,657 + HGI48_RS21435 0.52 -0.1
4,784,657 + HGI48_RS21435 0.52 -0.4
4,784,669 - +1.1
4,784,738 + HGI48_RS21435 0.56 -0.2
4,784,740 + HGI48_RS21435 0.56 +0.0
4,784,740 + HGI48_RS21435 0.56 -0.3
4,784,740 + HGI48_RS21435 0.56 +0.7
4,784,740 + HGI48_RS21435 0.56 +0.6
4,784,740 + HGI48_RS21435 0.56 -0.7
4,784,740 + HGI48_RS21435 0.56 +0.1
4,784,740 + HGI48_RS21435 0.56 +0.9
4,784,741 - HGI48_RS21435 0.56 -1.4
4,784,741 - HGI48_RS21435 0.56 -0.4
4,784,741 - HGI48_RS21435 0.56 -0.2
4,784,741 - HGI48_RS21435 0.56 -1.2
4,784,741 - HGI48_RS21435 0.56 +0.1
4,784,764 + +0.1
4,784,796 + HGI48_RS21435 0.59 +0.1
4,784,796 + HGI48_RS21435 0.59 +0.6
4,784,797 - HGI48_RS21435 0.59 +0.1
4,784,797 - +0.1
4,784,889 + HGI48_RS21435 0.63 +1.0
4,784,889 + HGI48_RS21435 0.63 -0.1
4,784,890 - HGI48_RS21435 0.63 -0.4
4,784,923 + HGI48_RS21435 0.65 +0.1
4,784,923 + HGI48_RS21435 0.65 -0.2
4,784,923 + HGI48_RS21435 0.65 +0.3
4,784,923 + HGI48_RS21435 0.65 -0.9
4,784,923 + HGI48_RS21435 0.65 -0.8
4,784,923 + HGI48_RS21435 0.65 +0.0
4,784,923 + HGI48_RS21435 0.65 -2.2
4,784,923 + HGI48_RS21435 0.65 -0.9
4,784,923 + +1.9
4,784,923 + HGI48_RS21435 0.65 -0.2
4,784,923 + HGI48_RS21435 0.65 -0.3
4,784,923 + HGI48_RS21435 0.65 +0.1
4,784,923 + HGI48_RS21435 0.65 +0.3
4,784,923 + HGI48_RS21435 0.65 +0.6
4,784,923 + +1.1
4,784,923 + HGI48_RS21435 0.65 +0.4
4,784,923 + +2.5
4,784,923 + -0.4
4,784,923 + +0.1
4,784,923 + HGI48_RS21435 0.65 +2.7
4,784,923 + HGI48_RS21435 0.65 +1.1
4,784,923 + HGI48_RS21435 0.65 -0.9
4,784,923 + HGI48_RS21435 0.65 -1.4
4,784,924 - HGI48_RS21435 0.65 -0.9
4,784,924 - HGI48_RS21435 0.65 -0.0
4,784,924 - HGI48_RS21435 0.65 -0.4
4,784,924 - HGI48_RS21435 0.65 -0.7
4,784,924 - HGI48_RS21435 0.65 -2.3
4,784,924 - HGI48_RS21435 0.65 +1.1
4,784,924 - HGI48_RS21435 0.65 -0.0
4,784,924 - HGI48_RS21435 0.65 -0.7
4,784,924 - HGI48_RS21435 0.65 -1.4
4,784,924 - HGI48_RS21435 0.65 +0.1
4,784,924 - HGI48_RS21435 0.65 -1.8
4,784,924 - HGI48_RS21435 0.65 +1.1
4,784,924 - HGI48_RS21435 0.65 -0.0
4,784,924 - HGI48_RS21435 0.65 -1.2
4,784,924 - HGI48_RS21435 0.65 -1.9
4,784,924 - HGI48_RS21435 0.65 -1.2
4,784,926 + HGI48_RS21435 0.65 -0.4
4,784,926 + HGI48_RS21435 0.65 +0.4
4,784,927 - HGI48_RS21435 0.65 -0.6
4,784,927 - +0.6
4,784,927 - HGI48_RS21435 0.65 -0.3
4,784,927 - HGI48_RS21435 0.65 -0.0
4,784,927 - HGI48_RS21435 0.65 +1.1
4,784,927 - HGI48_RS21435 0.65 +1.7
4,785,007 + HGI48_RS21435 0.68 +1.0
4,785,008 - HGI48_RS21435 0.68 -1.1
4,785,008 - HGI48_RS21435 0.68 -0.8
4,785,015 - HGI48_RS21435 0.69 -0.4
4,785,089 - HGI48_RS21435 0.72 +0.8
4,785,185 + HGI48_RS21435 0.76 -1.2
4,785,185 + HGI48_RS21435 0.76 +0.5
4,785,186 - HGI48_RS21435 0.76 +0.4
4,785,186 - HGI48_RS21435 0.76 -0.9
4,785,241 + HGI48_RS21435 0.79 -0.0
4,785,241 + HGI48_RS21435 0.79 +2.0
4,785,242 - +2.7
4,785,242 - HGI48_RS21435 0.79 -1.9
4,785,404 - HGI48_RS21435 0.86 -1.0
4,785,428 + HGI48_RS21435 0.87 -1.1
4,785,428 + HGI48_RS21435 0.87 +1.1
4,785,428 + HGI48_RS21435 0.87 -0.4
4,785,429 - HGI48_RS21435 0.87 +0.1
4,785,429 - +1.6
4,785,429 - HGI48_RS21435 0.87 +0.1
4,785,461 + +1.6
4,785,462 - HGI48_RS21435 0.89 +0.2
4,785,492 + -1.2
4,785,492 + -1.2
4,785,492 + +0.6
4,785,493 - +1.4
4,785,493 - -0.9
4,785,493 - +1.1
4,785,666 - -0.5
4,785,666 - +0.4
4,785,678 + +0.5
4,785,679 - -2.0
4,785,679 - +0.1
4,785,679 - +0.8
4,785,697 + -1.4
4,785,697 + -1.1
4,785,697 + +1.6
4,785,697 + +0.5
4,785,697 + +0.7
4,785,697 + +0.9
4,785,698 - +1.1
4,785,698 - +0.3
4,785,698 - -0.7
4,785,698 - -1.4
4,785,698 - +0.3
4,785,698 - -0.9
4,785,698 - +0.1
4,785,698 - -0.9
4,785,698 - +0.1
4,785,698 - -0.7
4,785,719 + +0.1
4,785,719 + +0.2
4,785,719 + -0.9
4,785,719 + -0.2
4,785,719 + +0.3
4,785,719 + +0.7
4,785,720 - -0.4
4,785,720 - -0.9
4,785,720 - +0.6
4,785,720 - -0.2
4,785,720 - +0.1
4,785,720 - +0.9
4,785,720 - +0.4
4,785,720 - -0.4
4,785,741 + +1.0
4,785,742 - +1.5
4,785,742 - +0.6
4,785,788 + -1.0
4,785,824 + -0.4
4,785,825 - -0.4
4,785,841 + -0.4
4,785,842 - +1.3
4,785,842 - +0.7
4,785,845 + +0.7
4,785,845 + -0.2
4,785,845 + -0.1
4,785,845 + +0.6
4,785,845 + -1.2
4,785,845 + +0.1
4,785,846 - -0.9
4,785,846 - -1.4
4,785,846 - -1.7
4,785,846 - +0.1
4,785,846 - -0.3
4,785,848 + -0.6
4,785,849 - +1.0
4,785,849 - -0.5

Or see this region's nucleotide sequence