Experiment: Atlantic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS05170 and HGI48_RS05175 are separated by 3 nucleotides HGI48_RS05175 and HGI48_RS05180 are separated by 3 nucleotides HGI48_RS05180 and HGI48_RS05185 overlap by 4 nucleotides HGI48_RS05185 and HGI48_RS05190 are separated by 75 nucleotides
HGI48_RS05170: HGI48_RS05170 - 3-hydroxyacyl-ACP dehydratase FabZ, at 1,084,542 to 1,084,997
_RS05170
HGI48_RS05175: HGI48_RS05175 - acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase, at 1,085,001 to 1,085,789
_RS05175
HGI48_RS05180: HGI48_RS05180 - lipid-A-disaccharide synthase, at 1,085,793 to 1,086,941
_RS05180
HGI48_RS05185: HGI48_RS05185 - ribonuclease HII, at 1,086,938 to 1,087,534
_RS05185
HGI48_RS05190: HGI48_RS05190 - DNA polymerase III subunit alpha, at 1,087,610 to 1,091,092
_RS05190
Position (kb) 1085
1086
1087 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1085.792 kb on + strand at 1085.792 kb on + strand at 1086.973 kb on + strand at 1086.974 kb on - strand at 1086.974 kb on - strand at 1086.974 kb on - strand at 1086.974 kb on - strand at 1086.993 kb on + strand at 1086.993 kb on + strand at 1086.994 kb on - strand at 1087.184 kb on - strand at 1087.445 kb on + strand, within HGI48_RS05185 at 1087.445 kb on + strand at 1087.445 kb on + strand at 1087.537 kb on + strand at 1087.554 kb on + strand at 1087.554 kb on + strand at 1087.554 kb on + strand at 1087.554 kb on + strand at 1087.554 kb on + strand at 1087.554 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Atlantic remove 1,085,792 + -3.6 1,085,792 + -2.9 1,086,973 + -2.6 1,086,974 - +0.8 1,086,974 - +1.3 1,086,974 - +0.7 1,086,974 - -1.6 1,086,993 + +0.2 1,086,993 + +3.6 1,086,994 - -0.3 1,087,184 - -0.0 1,087,445 + HGI48_RS05185 0.85 -0.0 1,087,445 + +2.0 1,087,445 + +1.7 1,087,537 + +3.2 1,087,554 + -0.2 1,087,554 + +0.9 1,087,554 + +0.1 1,087,554 + +0.7 1,087,554 + +0.6 1,087,554 + -0.7
Or see this region's nucleotide sequence