Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS11030 and HGI48_RS11035 are separated by 652 nucleotides HGI48_RS11035 and HGI48_RS11040 are separated by 262 nucleotides HGI48_RS11040 and HGI48_RS11045 overlap by 20 nucleotides
HGI48_RS11030: HGI48_RS11030 - IS3 family transposase, at 2,392,492 to 2,393,642
_RS11030
HGI48_RS11035: HGI48_RS11035 - DUF3732 domain-containing protein, at 2,394,295 to 2,394,903
_RS11035
HGI48_RS11040: HGI48_RS11040 - thermostable hemolysin, at 2,395,166 to 2,395,822
_RS11040
HGI48_RS11045: HGI48_RS11045 - AMP-binding protein, at 2,395,803 to 2,397,239
_RS11045
Position (kb)
2394
2395 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2393.657 kb on + strand at 2393.657 kb on + strand at 2393.674 kb on + strand at 2393.674 kb on + strand at 2393.674 kb on + strand at 2393.674 kb on + strand at 2393.674 kb on + strand at 2393.675 kb on - strand at 2393.676 kb on + strand at 2393.676 kb on + strand at 2393.677 kb on - strand at 2393.695 kb on + strand at 2393.696 kb on - strand at 2393.740 kb on + strand at 2393.764 kb on + strand at 2393.789 kb on - strand at 2393.816 kb on + strand at 2393.817 kb on - strand at 2393.862 kb on - strand at 2393.886 kb on + strand at 2393.887 kb on - strand at 2393.986 kb on - strand at 2393.996 kb on - strand at 2394.017 kb on + strand at 2394.041 kb on + strand at 2394.041 kb on + strand at 2394.042 kb on - strand at 2394.045 kb on + strand at 2394.045 kb on + strand at 2394.046 kb on - strand at 2394.046 kb on - strand at 2394.084 kb on + strand at 2394.127 kb on - strand at 2394.127 kb on - strand at 2394.143 kb on - strand at 2394.205 kb on + strand at 2394.208 kb on + strand at 2394.208 kb on + strand at 2394.208 kb on + strand at 2394.224 kb on + strand at 2394.225 kb on - strand at 2394.225 kb on - strand at 2394.227 kb on + strand at 2394.227 kb on + strand at 2394.228 kb on - strand at 2394.276 kb on - strand at 2394.276 kb on - strand at 2394.290 kb on + strand at 2394.290 kb on + strand at 2394.291 kb on - strand at 2394.293 kb on + strand at 2394.294 kb on - strand at 2394.316 kb on - strand at 2394.381 kb on - strand, within HGI48_RS11035 at 2394.389 kb on + strand, within HGI48_RS11035 at 2394.393 kb on + strand, within HGI48_RS11035 at 2394.428 kb on - strand, within HGI48_RS11035 at 2394.430 kb on - strand, within HGI48_RS11035 at 2394.470 kb on + strand, within HGI48_RS11035 at 2394.470 kb on + strand, within HGI48_RS11035 at 2394.471 kb on - strand at 2394.471 kb on - strand, within HGI48_RS11035 at 2394.471 kb on - strand, within HGI48_RS11035 at 2394.477 kb on - strand, within HGI48_RS11035 at 2394.477 kb on - strand, within HGI48_RS11035 at 2394.477 kb on - strand, within HGI48_RS11035 at 2394.477 kb on - strand, within HGI48_RS11035 at 2394.535 kb on + strand, within HGI48_RS11035 at 2394.536 kb on - strand, within HGI48_RS11035 at 2394.536 kb on - strand, within HGI48_RS11035 at 2394.542 kb on - strand, within HGI48_RS11035 at 2394.542 kb on - strand, within HGI48_RS11035 at 2394.551 kb on + strand at 2394.569 kb on + strand, within HGI48_RS11035 at 2394.569 kb on + strand, within HGI48_RS11035 at 2394.569 kb on + strand, within HGI48_RS11035 at 2394.569 kb on + strand, within HGI48_RS11035 at 2394.569 kb on + strand, within HGI48_RS11035 at 2394.569 kb on + strand, within HGI48_RS11035 at 2394.570 kb on - strand, within HGI48_RS11035 at 2394.570 kb on - strand at 2394.570 kb on - strand, within HGI48_RS11035 at 2394.570 kb on - strand, within HGI48_RS11035 at 2394.570 kb on - strand, within HGI48_RS11035 at 2394.686 kb on + strand, within HGI48_RS11035 at 2394.686 kb on + strand, within HGI48_RS11035 at 2394.686 kb on + strand, within HGI48_RS11035 at 2394.686 kb on + strand at 2394.686 kb on + strand, within HGI48_RS11035 at 2394.687 kb on - strand, within HGI48_RS11035 at 2394.687 kb on - strand, within HGI48_RS11035 at 2394.687 kb on - strand, within HGI48_RS11035 at 2394.687 kb on - strand, within HGI48_RS11035 at 2394.690 kb on + strand, within HGI48_RS11035 at 2394.690 kb on + strand, within HGI48_RS11035 at 2394.690 kb on + strand, within HGI48_RS11035 at 2394.690 kb on + strand at 2394.695 kb on + strand, within HGI48_RS11035 at 2394.696 kb on - strand, within HGI48_RS11035 at 2394.707 kb on + strand, within HGI48_RS11035 at 2394.754 kb on - strand, within HGI48_RS11035 at 2394.768 kb on + strand, within HGI48_RS11035 at 2394.768 kb on + strand, within HGI48_RS11035 at 2394.769 kb on - strand, within HGI48_RS11035 at 2394.769 kb on - strand, within HGI48_RS11035 at 2394.770 kb on + strand, within HGI48_RS11035 at 2394.770 kb on + strand, within HGI48_RS11035 at 2394.771 kb on - strand, within HGI48_RS11035 at 2394.771 kb on - strand, within HGI48_RS11035 at 2394.771 kb on - strand, within HGI48_RS11035 at 2394.774 kb on + strand, within HGI48_RS11035 at 2394.820 kb on + strand, within HGI48_RS11035 at 2394.839 kb on - strand, within HGI48_RS11035 at 2394.864 kb on + strand at 2394.865 kb on - strand at 2395.066 kb on + strand at 2395.066 kb on + strand at 2395.067 kb on - strand at 2395.110 kb on + strand at 2395.121 kb on + strand at 2395.127 kb on + strand at 2395.127 kb on + strand at 2395.162 kb on - strand at 2395.166 kb on - strand at 2395.226 kb on - strand at 2395.250 kb on + strand, within HGI48_RS11040 at 2395.262 kb on + strand, within HGI48_RS11040 at 2395.285 kb on + strand, within HGI48_RS11040 at 2395.286 kb on - strand, within HGI48_RS11040 at 2395.303 kb on + strand, within HGI48_RS11040 at 2395.308 kb on - strand, within HGI48_RS11040 at 2395.370 kb on + strand, within HGI48_RS11040 at 2395.370 kb on + strand at 2395.371 kb on - strand at 2395.371 kb on - strand, within HGI48_RS11040 at 2395.382 kb on + strand, within HGI48_RS11040 at 2395.382 kb on + strand, within HGI48_RS11040 at 2395.382 kb on + strand, within HGI48_RS11040 at 2395.383 kb on - strand, within HGI48_RS11040 at 2395.502 kb on + strand, within HGI48_RS11040 at 2395.502 kb on + strand, within HGI48_RS11040 at 2395.503 kb on - strand, within HGI48_RS11040 at 2395.503 kb on - strand, within HGI48_RS11040 at 2395.532 kb on + strand, within HGI48_RS11040 at 2395.552 kb on - strand, within HGI48_RS11040 at 2395.586 kb on + strand, within HGI48_RS11040 at 2395.609 kb on - strand, within HGI48_RS11040 at 2395.612 kb on - strand, within HGI48_RS11040 at 2395.612 kb on - strand, within HGI48_RS11040 at 2395.667 kb on + strand, within HGI48_RS11040 at 2395.667 kb on + strand, within HGI48_RS11040 at 2395.667 kb on + strand, within HGI48_RS11040 at 2395.667 kb on + strand, within HGI48_RS11040 at 2395.668 kb on - strand, within HGI48_RS11040 at 2395.668 kb on - strand, within HGI48_RS11040 at 2395.668 kb on - strand, within HGI48_RS11040 at 2395.668 kb on - strand, within HGI48_RS11040 at 2395.670 kb on + strand, within HGI48_RS11040 at 2395.671 kb on - strand at 2395.743 kb on + strand at 2395.743 kb on + strand, within HGI48_RS11040 at 2395.757 kb on - strand at 2395.802 kb on + strand at 2395.802 kb on + strand at 2395.802 kb on + strand at 2395.802 kb on + strand at 2395.802 kb on + strand at 2395.803 kb on - strand at 2395.803 kb on - strand at 2395.893 kb on + strand at 2395.894 kb on - strand at 2395.894 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 2,393,657 + +1.2 2,393,657 + +0.6 2,393,674 + -1.7 2,393,674 + -1.5 2,393,674 + +0.2 2,393,674 + +0.2 2,393,674 + +0.6 2,393,675 - +0.3 2,393,676 + -0.1 2,393,676 + +1.6 2,393,677 - -2.5 2,393,695 + +0.0 2,393,696 - -1.7 2,393,740 + +1.6 2,393,764 + -1.4 2,393,789 - +2.5 2,393,816 + +0.3 2,393,817 - +0.3 2,393,862 - +0.3 2,393,886 + -0.9 2,393,887 - +0.6 2,393,986 - -0.5 2,393,996 - -1.9 2,394,017 + -0.7 2,394,041 + -0.5 2,394,041 + +0.9 2,394,042 - +0.3 2,394,045 + +0.2 2,394,045 + -0.0 2,394,046 - -1.6 2,394,046 - +1.6 2,394,084 + +0.1 2,394,127 - +0.4 2,394,127 - +0.2 2,394,143 - -1.9 2,394,205 + +0.2 2,394,208 + -0.9 2,394,208 + -0.2 2,394,208 + +1.1 2,394,224 + -0.6 2,394,225 - -0.1 2,394,225 - -1.0 2,394,227 + -1.7 2,394,227 + -0.6 2,394,228 - -1.4 2,394,276 - -0.9 2,394,276 - -0.4 2,394,290 + -0.0 2,394,290 + -2.4 2,394,291 - +0.6 2,394,293 + +0.2 2,394,294 - -0.9 2,394,316 - -0.4 2,394,381 - HGI48_RS11035 0.14 +0.0 2,394,389 + HGI48_RS11035 0.15 +0.9 2,394,393 + HGI48_RS11035 0.16 -0.9 2,394,428 - HGI48_RS11035 0.22 -0.4 2,394,430 - HGI48_RS11035 0.22 -1.2 2,394,470 + HGI48_RS11035 0.29 +1.0 2,394,470 + HGI48_RS11035 0.29 +2.0 2,394,471 - +1.1 2,394,471 - HGI48_RS11035 0.29 +0.9 2,394,471 - HGI48_RS11035 0.29 -1.9 2,394,477 - HGI48_RS11035 0.30 -0.4 2,394,477 - HGI48_RS11035 0.30 -0.7 2,394,477 - HGI48_RS11035 0.30 +0.3 2,394,477 - HGI48_RS11035 0.30 -1.6 2,394,535 + HGI48_RS11035 0.39 -0.2 2,394,536 - HGI48_RS11035 0.40 -0.4 2,394,536 - HGI48_RS11035 0.40 -0.8 2,394,542 - HGI48_RS11035 0.41 -0.7 2,394,542 - HGI48_RS11035 0.41 -0.4 2,394,551 + +1.4 2,394,569 + HGI48_RS11035 0.45 -0.5 2,394,569 + HGI48_RS11035 0.45 -1.7 2,394,569 + HGI48_RS11035 0.45 -1.4 2,394,569 + HGI48_RS11035 0.45 -0.8 2,394,569 + HGI48_RS11035 0.45 -0.8 2,394,569 + HGI48_RS11035 0.45 +1.1 2,394,570 - HGI48_RS11035 0.45 -0.1 2,394,570 - -0.9 2,394,570 - HGI48_RS11035 0.45 +1.3 2,394,570 - HGI48_RS11035 0.45 +1.1 2,394,570 - HGI48_RS11035 0.45 +0.9 2,394,686 + HGI48_RS11035 0.64 +1.9 2,394,686 + HGI48_RS11035 0.64 -0.4 2,394,686 + HGI48_RS11035 0.64 -0.2 2,394,686 + +1.1 2,394,686 + HGI48_RS11035 0.64 -1.3 2,394,687 - HGI48_RS11035 0.64 +0.1 2,394,687 - HGI48_RS11035 0.64 -1.5 2,394,687 - HGI48_RS11035 0.64 -1.5 2,394,687 - HGI48_RS11035 0.64 +0.3 2,394,690 + HGI48_RS11035 0.65 -0.4 2,394,690 + HGI48_RS11035 0.65 -0.4 2,394,690 + HGI48_RS11035 0.65 -0.1 2,394,690 + -0.4 2,394,695 + HGI48_RS11035 0.66 -0.9 2,394,696 - HGI48_RS11035 0.66 +1.1 2,394,707 + HGI48_RS11035 0.68 -1.4 2,394,754 - HGI48_RS11035 0.75 +0.6 2,394,768 + HGI48_RS11035 0.78 +2.3 2,394,768 + HGI48_RS11035 0.78 -0.1 2,394,769 - HGI48_RS11035 0.78 -1.4 2,394,769 - HGI48_RS11035 0.78 +0.1 2,394,770 + HGI48_RS11035 0.78 -2.7 2,394,770 + HGI48_RS11035 0.78 -1.4 2,394,771 - HGI48_RS11035 0.78 -1.4 2,394,771 - HGI48_RS11035 0.78 -0.2 2,394,771 - HGI48_RS11035 0.78 -2.4 2,394,774 + HGI48_RS11035 0.79 -0.2 2,394,820 + HGI48_RS11035 0.86 -1.9 2,394,839 - HGI48_RS11035 0.89 +1.6 2,394,864 + +1.1 2,394,865 - -0.1 2,395,066 + +0.6 2,395,066 + +1.6 2,395,067 - -0.5 2,395,110 + +0.1 2,395,121 + -0.4 2,395,127 + +0.1 2,395,127 + +0.5 2,395,162 - +0.1 2,395,166 - -0.4 2,395,226 - -1.2 2,395,250 + HGI48_RS11040 0.13 +0.6 2,395,262 + HGI48_RS11040 0.15 -1.2 2,395,285 + HGI48_RS11040 0.18 +0.3 2,395,286 - HGI48_RS11040 0.18 +1.5 2,395,303 + HGI48_RS11040 0.21 -0.0 2,395,308 - HGI48_RS11040 0.22 -2.4 2,395,370 + HGI48_RS11040 0.31 +0.9 2,395,370 + -0.9 2,395,371 - +0.6 2,395,371 - HGI48_RS11040 0.31 -0.2 2,395,382 + HGI48_RS11040 0.33 -0.4 2,395,382 + HGI48_RS11040 0.33 +1.1 2,395,382 + HGI48_RS11040 0.33 -0.8 2,395,383 - HGI48_RS11040 0.33 -0.2 2,395,502 + HGI48_RS11040 0.51 +0.1 2,395,502 + HGI48_RS11040 0.51 +0.1 2,395,503 - HGI48_RS11040 0.51 -1.5 2,395,503 - HGI48_RS11040 0.51 -0.9 2,395,532 + HGI48_RS11040 0.56 +0.4 2,395,552 - HGI48_RS11040 0.59 -0.5 2,395,586 + HGI48_RS11040 0.64 -0.4 2,395,609 - HGI48_RS11040 0.67 -0.0 2,395,612 - HGI48_RS11040 0.68 -0.9 2,395,612 - HGI48_RS11040 0.68 -2.2 2,395,667 + HGI48_RS11040 0.76 -1.4 2,395,667 + HGI48_RS11040 0.76 -2.2 2,395,667 + HGI48_RS11040 0.76 -0.5 2,395,667 + HGI48_RS11040 0.76 +0.4 2,395,668 - HGI48_RS11040 0.76 -1.7 2,395,668 - HGI48_RS11040 0.76 -2.5 2,395,668 - HGI48_RS11040 0.76 -0.1 2,395,668 - HGI48_RS11040 0.76 -0.0 2,395,670 + HGI48_RS11040 0.77 +2.0 2,395,671 - -0.9 2,395,743 + +0.1 2,395,743 + HGI48_RS11040 0.88 +0.0 2,395,757 - -2.5 2,395,802 + +0.4 2,395,802 + +0.6 2,395,802 + -0.4 2,395,802 + -2.2 2,395,802 + -0.7 2,395,803 - -0.2 2,395,803 - -2.5 2,395,893 + +1.4 2,395,894 - -0.2 2,395,894 - +0.6
Or see this region's nucleotide sequence