Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01620 and HGI48_RS01625 are separated by 170 nucleotides HGI48_RS01625 and HGI48_RS01630 are separated by 11 nucleotides HGI48_RS01630 and HGI48_RS01635 are separated by 176 nucleotides
HGI48_RS01620: HGI48_RS01620 - 4,5-DOPA dioxygenase extradiol, at 357,285 to 358,073
_RS01620
HGI48_RS01625: HGI48_RS01625 - glutathionylspermidine synthase family protein, at 358,244 to 359,404
_RS01625
HGI48_RS01630: HGI48_RS01630 - DUF1190 family protein, at 359,416 to 360,168
_RS01630
HGI48_RS01635: HGI48_RS01635 - outer membrane channel protein TolC, at 360,345 to 361,757
_RS01635
Position (kb) 358
359
360 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 357.250 kb on + strand at 357.250 kb on + strand at 357.251 kb on - strand at 357.254 kb on + strand at 357.254 kb on + strand at 357.254 kb on + strand at 357.254 kb on + strand at 357.254 kb on + strand at 357.254 kb on + strand at 357.255 kb on - strand at 357.272 kb on + strand at 357.278 kb on + strand at 357.278 kb on + strand at 357.278 kb on + strand at 357.357 kb on + strand at 357.358 kb on - strand at 357.375 kb on - strand, within HGI48_RS01620 at 357.432 kb on + strand, within HGI48_RS01620 at 357.432 kb on + strand, within HGI48_RS01620 at 357.432 kb on + strand, within HGI48_RS01620 at 357.432 kb on + strand, within HGI48_RS01620 at 357.432 kb on + strand, within HGI48_RS01620 at 357.433 kb on - strand, within HGI48_RS01620 at 357.433 kb on - strand, within HGI48_RS01620 at 357.433 kb on - strand, within HGI48_RS01620 at 357.433 kb on - strand, within HGI48_RS01620 at 357.434 kb on + strand, within HGI48_RS01620 at 357.464 kb on + strand, within HGI48_RS01620 at 357.588 kb on + strand, within HGI48_RS01620 at 357.588 kb on + strand, within HGI48_RS01620 at 357.680 kb on + strand, within HGI48_RS01620 at 357.681 kb on - strand, within HGI48_RS01620 at 357.681 kb on - strand, within HGI48_RS01620 at 357.681 kb on - strand, within HGI48_RS01620 at 357.681 kb on - strand, within HGI48_RS01620 at 357.681 kb on - strand, within HGI48_RS01620 at 357.708 kb on + strand, within HGI48_RS01620 at 357.829 kb on - strand, within HGI48_RS01620 at 357.914 kb on + strand, within HGI48_RS01620 at 357.915 kb on - strand, within HGI48_RS01620 at 357.915 kb on - strand, within HGI48_RS01620 at 357.975 kb on + strand, within HGI48_RS01620 at 357.975 kb on + strand, within HGI48_RS01620 at 357.975 kb on + strand, within HGI48_RS01620 at 357.975 kb on + strand, within HGI48_RS01620 at 357.975 kb on + strand, within HGI48_RS01620 at 357.976 kb on - strand, within HGI48_RS01620 at 357.976 kb on - strand, within HGI48_RS01620 at 357.976 kb on - strand, within HGI48_RS01620 at 357.976 kb on - strand, within HGI48_RS01620 at 357.976 kb on - strand at 357.976 kb on - strand, within HGI48_RS01620 at 357.976 kb on - strand, within HGI48_RS01620 at 358.102 kb on + strand at 358.120 kb on + strand at 358.141 kb on + strand at 358.141 kb on + strand at 358.142 kb on - strand at 358.142 kb on - strand at 358.142 kb on - strand at 358.161 kb on - strand at 358.161 kb on - strand at 358.161 kb on - strand at 358.180 kb on - strand at 358.180 kb on - strand at 358.199 kb on - strand at 358.217 kb on + strand at 358.217 kb on + strand at 358.218 kb on - strand at 358.259 kb on - strand at 358.259 kb on - strand at 358.266 kb on + strand at 358.360 kb on + strand, within HGI48_RS01625 at 358.361 kb on - strand, within HGI48_RS01625 at 358.361 kb on - strand, within HGI48_RS01625 at 358.361 kb on - strand, within HGI48_RS01625 at 358.361 kb on - strand, within HGI48_RS01625 at 358.362 kb on + strand at 358.362 kb on + strand, within HGI48_RS01625 at 358.362 kb on + strand, within HGI48_RS01625 at 358.362 kb on + strand, within HGI48_RS01625 at 358.362 kb on + strand, within HGI48_RS01625 at 358.362 kb on + strand, within HGI48_RS01625 at 358.362 kb on + strand at 358.362 kb on + strand, within HGI48_RS01625 at 358.363 kb on - strand, within HGI48_RS01625 at 358.363 kb on - strand, within HGI48_RS01625 at 358.363 kb on - strand, within HGI48_RS01625 at 358.363 kb on - strand at 358.363 kb on - strand, within HGI48_RS01625 at 358.363 kb on - strand, within HGI48_RS01625 at 358.461 kb on + strand, within HGI48_RS01625 at 358.461 kb on + strand, within HGI48_RS01625 at 358.462 kb on - strand, within HGI48_RS01625 at 358.469 kb on + strand, within HGI48_RS01625 at 358.469 kb on + strand, within HGI48_RS01625 at 358.469 kb on + strand, within HGI48_RS01625 at 358.469 kb on + strand, within HGI48_RS01625 at 358.469 kb on + strand, within HGI48_RS01625 at 358.469 kb on + strand, within HGI48_RS01625 at 358.469 kb on + strand, within HGI48_RS01625 at 358.470 kb on - strand, within HGI48_RS01625 at 358.470 kb on - strand, within HGI48_RS01625 at 358.470 kb on - strand, within HGI48_RS01625 at 358.470 kb on - strand, within HGI48_RS01625 at 358.472 kb on - strand, within HGI48_RS01625 at 358.509 kb on + strand, within HGI48_RS01625 at 358.509 kb on + strand, within HGI48_RS01625 at 358.509 kb on + strand, within HGI48_RS01625 at 358.509 kb on + strand at 358.510 kb on - strand, within HGI48_RS01625 at 358.510 kb on - strand, within HGI48_RS01625 at 358.545 kb on + strand, within HGI48_RS01625 at 358.546 kb on - strand, within HGI48_RS01625 at 358.546 kb on - strand, within HGI48_RS01625 at 358.739 kb on + strand, within HGI48_RS01625 at 358.740 kb on - strand, within HGI48_RS01625 at 358.785 kb on + strand, within HGI48_RS01625 at 358.785 kb on + strand, within HGI48_RS01625 at 358.786 kb on - strand at 358.786 kb on - strand, within HGI48_RS01625 at 358.833 kb on + strand, within HGI48_RS01625 at 358.834 kb on - strand, within HGI48_RS01625 at 358.834 kb on - strand, within HGI48_RS01625 at 358.834 kb on - strand, within HGI48_RS01625 at 358.834 kb on - strand, within HGI48_RS01625 at 358.843 kb on + strand, within HGI48_RS01625 at 358.844 kb on - strand, within HGI48_RS01625 at 358.886 kb on - strand, within HGI48_RS01625 at 358.886 kb on - strand, within HGI48_RS01625 at 358.886 kb on - strand, within HGI48_RS01625 at 358.886 kb on - strand, within HGI48_RS01625 at 358.914 kb on + strand, within HGI48_RS01625 at 358.915 kb on - strand, within HGI48_RS01625 at 358.948 kb on + strand, within HGI48_RS01625 at 358.949 kb on - strand, within HGI48_RS01625 at 358.949 kb on - strand at 358.949 kb on - strand, within HGI48_RS01625 at 358.953 kb on + strand, within HGI48_RS01625 at 358.953 kb on + strand, within HGI48_RS01625 at 358.954 kb on - strand, within HGI48_RS01625 at 358.954 kb on - strand, within HGI48_RS01625 at 358.954 kb on - strand, within HGI48_RS01625 at 359.091 kb on + strand, within HGI48_RS01625 at 359.112 kb on + strand, within HGI48_RS01625 at 359.113 kb on - strand at 359.113 kb on - strand, within HGI48_RS01625 at 359.292 kb on + strand at 359.292 kb on + strand at 359.292 kb on + strand at 359.293 kb on - strand at 359.293 kb on - strand at 359.295 kb on + strand at 359.295 kb on + strand at 359.295 kb on + strand at 359.296 kb on - strand at 359.296 kb on - strand at 359.296 kb on - strand at 359.313 kb on + strand at 359.325 kb on + strand at 359.325 kb on + strand at 359.325 kb on + strand at 359.325 kb on + strand at 359.325 kb on + strand at 359.325 kb on + strand at 359.325 kb on + strand at 359.325 kb on + strand at 359.326 kb on - strand at 359.326 kb on - strand at 359.326 kb on - strand at 359.326 kb on - strand at 359.349 kb on + strand at 359.395 kb on + strand at 359.395 kb on + strand at 359.395 kb on + strand at 359.395 kb on + strand at 359.396 kb on - strand at 359.396 kb on - strand at 359.502 kb on + strand at 359.503 kb on - strand, within HGI48_RS01630 at 359.601 kb on + strand, within HGI48_RS01630 at 359.646 kb on + strand, within HGI48_RS01630 at 359.687 kb on + strand, within HGI48_RS01630 at 359.687 kb on + strand, within HGI48_RS01630 at 359.687 kb on + strand, within HGI48_RS01630 at 359.688 kb on - strand, within HGI48_RS01630 at 359.688 kb on - strand, within HGI48_RS01630 at 359.711 kb on + strand at 359.711 kb on + strand, within HGI48_RS01630 at 359.711 kb on + strand, within HGI48_RS01630 at 359.711 kb on + strand, within HGI48_RS01630 at 359.711 kb on + strand, within HGI48_RS01630 at 359.712 kb on - strand, within HGI48_RS01630 at 359.712 kb on - strand, within HGI48_RS01630 at 359.712 kb on - strand, within HGI48_RS01630 at 359.712 kb on - strand, within HGI48_RS01630 at 359.712 kb on - strand at 359.712 kb on - strand, within HGI48_RS01630 at 359.712 kb on - strand at 359.730 kb on + strand, within HGI48_RS01630 at 359.731 kb on - strand, within HGI48_RS01630 at 359.733 kb on + strand, within HGI48_RS01630 at 359.799 kb on + strand, within HGI48_RS01630 at 359.927 kb on + strand, within HGI48_RS01630 at 359.927 kb on + strand, within HGI48_RS01630 at 359.927 kb on + strand, within HGI48_RS01630 at 359.968 kb on - strand, within HGI48_RS01630 at 359.968 kb on - strand, within HGI48_RS01630 at 359.969 kb on + strand, within HGI48_RS01630 at 359.969 kb on + strand, within HGI48_RS01630 at 359.969 kb on + strand, within HGI48_RS01630 at 359.969 kb on + strand, within HGI48_RS01630 at 359.970 kb on - strand, within HGI48_RS01630 at 359.970 kb on - strand, within HGI48_RS01630 at 359.979 kb on + strand, within HGI48_RS01630 at 359.980 kb on - strand, within HGI48_RS01630 at 360.040 kb on + strand, within HGI48_RS01630 at 360.076 kb on - strand, within HGI48_RS01630 at 360.217 kb on + strand at 360.217 kb on + strand at 360.217 kb on + strand at 360.218 kb on - strand at 360.218 kb on - strand at 360.218 kb on - strand at 360.219 kb on + strand at 360.220 kb on - strand at 360.220 kb on - strand at 360.220 kb on - strand at 360.230 kb on + strand at 360.236 kb on + strand at 360.237 kb on - strand at 360.237 kb on - strand at 360.258 kb on + strand at 360.258 kb on + strand at 360.259 kb on - strand at 360.306 kb on + strand at 360.307 kb on - strand at 360.343 kb on + strand at 360.343 kb on + strand at 360.343 kb on + strand at 360.343 kb on + strand at 360.343 kb on + strand at 360.344 kb on - strand at 360.344 kb on - strand at 360.344 kb on - strand at 360.344 kb on - strand at 360.346 kb on + strand at 360.347 kb on - strand at 360.347 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 357,250 + -1.2 357,250 + -2.8 357,251 - -0.2 357,254 + +1.1 357,254 + +0.7 357,254 + -2.4 357,254 + +1.1 357,254 + -1.2 357,254 + +0.3 357,255 - +0.2 357,272 + +0.5 357,278 + -0.2 357,278 + -0.8 357,278 + +0.3 357,357 + +0.1 357,358 - -0.2 357,375 - HGI48_RS01620 0.11 +0.8 357,432 + HGI48_RS01620 0.19 -0.4 357,432 + HGI48_RS01620 0.19 -0.1 357,432 + HGI48_RS01620 0.19 +0.2 357,432 + HGI48_RS01620 0.19 -2.0 357,432 + HGI48_RS01620 0.19 -1.9 357,433 - HGI48_RS01620 0.19 -1.7 357,433 - HGI48_RS01620 0.19 -1.3 357,433 - HGI48_RS01620 0.19 +0.1 357,433 - HGI48_RS01620 0.19 +0.9 357,434 + HGI48_RS01620 0.19 +0.8 357,464 + HGI48_RS01620 0.23 -1.2 357,588 + HGI48_RS01620 0.38 -2.1 357,588 + HGI48_RS01620 0.38 -2.7 357,680 + HGI48_RS01620 0.50 -0.2 357,681 - HGI48_RS01620 0.50 +1.1 357,681 - HGI48_RS01620 0.50 +0.1 357,681 - HGI48_RS01620 0.50 -1.5 357,681 - HGI48_RS01620 0.50 +0.7 357,681 - HGI48_RS01620 0.50 +0.1 357,708 + HGI48_RS01620 0.54 +1.4 357,829 - HGI48_RS01620 0.69 -0.2 357,914 + HGI48_RS01620 0.80 -0.6 357,915 - HGI48_RS01620 0.80 -0.3 357,915 - HGI48_RS01620 0.80 +0.1 357,975 + HGI48_RS01620 0.87 -0.5 357,975 + HGI48_RS01620 0.87 -1.7 357,975 + HGI48_RS01620 0.87 +0.7 357,975 + HGI48_RS01620 0.87 +0.1 357,975 + HGI48_RS01620 0.87 -0.2 357,976 - HGI48_RS01620 0.88 -0.5 357,976 - HGI48_RS01620 0.88 +1.0 357,976 - HGI48_RS01620 0.88 +1.5 357,976 - HGI48_RS01620 0.88 -1.4 357,976 - -0.5 357,976 - HGI48_RS01620 0.88 -1.1 357,976 - HGI48_RS01620 0.88 +0.3 358,102 + +0.6 358,120 + -2.7 358,141 + -0.9 358,141 + -0.0 358,142 - +0.3 358,142 - -1.9 358,142 - -0.9 358,161 - -0.5 358,161 - -0.2 358,161 - -0.5 358,180 - -0.5 358,180 - +3.1 358,199 - -1.3 358,217 + +1.1 358,217 + -1.2 358,218 - +0.3 358,259 - +0.1 358,259 - -1.1 358,266 + -0.5 358,360 + HGI48_RS01625 0.10 +0.1 358,361 - HGI48_RS01625 0.10 +1.8 358,361 - HGI48_RS01625 0.10 +1.1 358,361 - HGI48_RS01625 0.10 +2.6 358,361 - HGI48_RS01625 0.10 +0.3 358,362 + -0.5 358,362 + HGI48_RS01625 0.10 +0.5 358,362 + HGI48_RS01625 0.10 -0.1 358,362 + HGI48_RS01625 0.10 -0.9 358,362 + HGI48_RS01625 0.10 +0.1 358,362 + HGI48_RS01625 0.10 -0.2 358,362 + +2.1 358,362 + HGI48_RS01625 0.10 +0.2 358,363 - HGI48_RS01625 0.10 -1.2 358,363 - HGI48_RS01625 0.10 -0.9 358,363 - HGI48_RS01625 0.10 -1.0 358,363 - +1.1 358,363 - HGI48_RS01625 0.10 -0.9 358,363 - HGI48_RS01625 0.10 -0.4 358,461 + HGI48_RS01625 0.19 +0.7 358,461 + HGI48_RS01625 0.19 -0.6 358,462 - HGI48_RS01625 0.19 -2.8 358,469 + HGI48_RS01625 0.19 -0.2 358,469 + HGI48_RS01625 0.19 +0.1 358,469 + HGI48_RS01625 0.19 -0.9 358,469 + HGI48_RS01625 0.19 +0.7 358,469 + HGI48_RS01625 0.19 -2.0 358,469 + HGI48_RS01625 0.19 -1.5 358,469 + HGI48_RS01625 0.19 -1.2 358,470 - HGI48_RS01625 0.19 -0.3 358,470 - HGI48_RS01625 0.19 +0.3 358,470 - HGI48_RS01625 0.19 +0.3 358,470 - HGI48_RS01625 0.19 -1.9 358,472 - HGI48_RS01625 0.20 -0.5 358,509 + HGI48_RS01625 0.23 -1.2 358,509 + HGI48_RS01625 0.23 -0.3 358,509 + HGI48_RS01625 0.23 +0.5 358,509 + -0.5 358,510 - HGI48_RS01625 0.23 +0.8 358,510 - HGI48_RS01625 0.23 -0.5 358,545 + HGI48_RS01625 0.26 -1.5 358,546 - HGI48_RS01625 0.26 +0.7 358,546 - HGI48_RS01625 0.26 +0.2 358,739 + HGI48_RS01625 0.43 +1.1 358,740 - HGI48_RS01625 0.43 +0.2 358,785 + HGI48_RS01625 0.47 +0.3 358,785 + HGI48_RS01625 0.47 +1.4 358,786 - +2.1 358,786 - HGI48_RS01625 0.47 -0.7 358,833 + HGI48_RS01625 0.51 -1.1 358,834 - HGI48_RS01625 0.51 +0.9 358,834 - HGI48_RS01625 0.51 +1.0 358,834 - HGI48_RS01625 0.51 +0.9 358,834 - HGI48_RS01625 0.51 -0.5 358,843 + HGI48_RS01625 0.52 -0.2 358,844 - HGI48_RS01625 0.52 -1.1 358,886 - HGI48_RS01625 0.55 -1.5 358,886 - HGI48_RS01625 0.55 +0.1 358,886 - HGI48_RS01625 0.55 -0.2 358,886 - HGI48_RS01625 0.55 +0.9 358,914 + HGI48_RS01625 0.58 -0.7 358,915 - HGI48_RS01625 0.58 -1.9 358,948 + HGI48_RS01625 0.61 +0.1 358,949 - HGI48_RS01625 0.61 +0.3 358,949 - +3.1 358,949 - HGI48_RS01625 0.61 +0.3 358,953 + HGI48_RS01625 0.61 -0.7 358,953 + HGI48_RS01625 0.61 -2.8 358,954 - HGI48_RS01625 0.61 -2.5 358,954 - HGI48_RS01625 0.61 -0.4 358,954 - HGI48_RS01625 0.61 -0.2 359,091 + HGI48_RS01625 0.73 -1.9 359,112 + HGI48_RS01625 0.75 +2.1 359,113 - +1.7 359,113 - HGI48_RS01625 0.75 +0.3 359,292 + -0.5 359,292 + -0.9 359,292 + -1.2 359,293 - -0.8 359,293 - -1.6 359,295 + -0.7 359,295 + -1.2 359,295 + +1.2 359,296 - -1.2 359,296 - +0.2 359,296 - -1.7 359,313 + -2.6 359,325 + -0.4 359,325 + +0.2 359,325 + -0.1 359,325 + -0.2 359,325 + -0.4 359,325 + +2.3 359,325 + -2.4 359,325 + -1.5 359,326 - -0.6 359,326 - +0.8 359,326 - +2.1 359,326 - -0.2 359,349 + +1.1 359,395 + -1.5 359,395 + +2.1 359,395 + -1.1 359,395 + -2.8 359,396 - +0.1 359,396 - -0.6 359,502 + +1.8 359,503 - HGI48_RS01630 0.12 -0.2 359,601 + HGI48_RS01630 0.25 -0.5 359,646 + HGI48_RS01630 0.31 +0.8 359,687 + HGI48_RS01630 0.36 -0.8 359,687 + HGI48_RS01630 0.36 +0.1 359,687 + HGI48_RS01630 0.36 +1.5 359,688 - HGI48_RS01630 0.36 -0.2 359,688 - HGI48_RS01630 0.36 -0.3 359,711 + +0.1 359,711 + HGI48_RS01630 0.39 +0.7 359,711 + HGI48_RS01630 0.39 -1.1 359,711 + HGI48_RS01630 0.39 -2.4 359,711 + HGI48_RS01630 0.39 +1.1 359,712 - HGI48_RS01630 0.39 +1.3 359,712 - HGI48_RS01630 0.39 -1.7 359,712 - HGI48_RS01630 0.39 -1.9 359,712 - HGI48_RS01630 0.39 +1.9 359,712 - -0.5 359,712 - HGI48_RS01630 0.39 -1.2 359,712 - +2.7 359,730 + HGI48_RS01630 0.42 -0.2 359,731 - HGI48_RS01630 0.42 -1.0 359,733 + HGI48_RS01630 0.42 -0.2 359,799 + HGI48_RS01630 0.51 +1.1 359,927 + HGI48_RS01630 0.68 +1.1 359,927 + HGI48_RS01630 0.68 +2.2 359,927 + HGI48_RS01630 0.68 +1.1 359,968 - HGI48_RS01630 0.73 -1.2 359,968 - HGI48_RS01630 0.73 +1.6 359,969 + HGI48_RS01630 0.73 -0.9 359,969 + HGI48_RS01630 0.73 +1.1 359,969 + HGI48_RS01630 0.73 -0.5 359,969 + HGI48_RS01630 0.73 +1.9 359,970 - HGI48_RS01630 0.74 +0.1 359,970 - HGI48_RS01630 0.74 -1.5 359,979 + HGI48_RS01630 0.75 +0.8 359,980 - HGI48_RS01630 0.75 -1.7 360,040 + HGI48_RS01630 0.83 -0.9 360,076 - HGI48_RS01630 0.88 -1.1 360,217 + +0.7 360,217 + -0.5 360,217 + +0.5 360,218 - -1.5 360,218 - -1.5 360,218 - -2.1 360,219 + -0.2 360,220 - -1.5 360,220 - -1.7 360,220 - -2.4 360,230 + +0.5 360,236 + +0.4 360,237 - -0.5 360,237 - +1.7 360,258 + +1.8 360,258 + +1.5 360,259 - +0.1 360,306 + +0.3 360,307 - +0.9 360,343 + +0.7 360,343 + -1.0 360,343 + -1.5 360,343 + +0.5 360,343 + -1.9 360,344 - +0.5 360,344 - +0.7 360,344 - -0.9 360,344 - +0.7 360,346 + -1.5 360,347 - -0.2 360,347 - -0.5
Or see this region's nucleotide sequence