Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS18335 and HGI48_RS18340 are separated by 3 nucleotides HGI48_RS18340 and HGI48_RS18345 are separated by 2 nucleotides HGI48_RS18345 and HGI48_RS18350 are separated by 433 nucleotides
HGI48_RS18335: HGI48_RS18335 - threonine synthase, at 4,114,958 to 4,116,247
_RS18335
HGI48_RS18340: HGI48_RS18340 - homoserine kinase, at 4,116,251 to 4,117,180
_RS18340
HGI48_RS18345: HGI48_RS18345 - bifunctional aspartate kinase/homoserine dehydrogenase I, at 4,117,183 to 4,119,639
_RS18345
HGI48_RS18350: HGI48_RS18350 - tRNA/rRNA methyltransferase, at 4,120,073 to 4,120,774
_RS18350
Position (kb)
4117
4118
4119
4120 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4116.237 kb on + strand at 4116.238 kb on - strand at 4116.238 kb on - strand at 4116.238 kb on - strand at 4116.292 kb on + strand at 4116.292 kb on + strand at 4116.292 kb on + strand at 4116.292 kb on + strand at 4116.292 kb on + strand at 4116.292 kb on + strand at 4116.293 kb on - strand at 4116.293 kb on - strand at 4116.293 kb on - strand at 4116.293 kb on - strand at 4116.293 kb on - strand at 4116.321 kb on + strand at 4116.322 kb on - strand at 4116.322 kb on - strand at 4116.762 kb on + strand, within HGI48_RS18340 at 4116.762 kb on + strand, within HGI48_RS18340 at 4116.984 kb on + strand, within HGI48_RS18340 at 4117.048 kb on + strand, within HGI48_RS18340 at 4117.049 kb on - strand, within HGI48_RS18340 at 4117.167 kb on + strand at 4117.167 kb on + strand at 4117.209 kb on + strand at 4117.210 kb on - strand at 4117.292 kb on + strand at 4117.295 kb on + strand at 4117.296 kb on - strand at 4117.304 kb on + strand at 4117.409 kb on + strand at 4117.409 kb on + strand at 4117.409 kb on + strand at 4117.409 kb on + strand at 4117.414 kb on + strand at 4117.422 kb on + strand at 4117.422 kb on + strand at 4117.512 kb on + strand, within HGI48_RS18345 at 4117.512 kb on + strand, within HGI48_RS18345 at 4117.512 kb on + strand at 4117.513 kb on - strand, within HGI48_RS18345 at 4117.513 kb on - strand, within HGI48_RS18345 at 4117.566 kb on - strand, within HGI48_RS18345 at 4117.713 kb on + strand, within HGI48_RS18345 at 4117.738 kb on - strand at 4117.826 kb on + strand, within HGI48_RS18345 at 4117.826 kb on + strand at 4117.827 kb on - strand, within HGI48_RS18345 at 4117.827 kb on - strand, within HGI48_RS18345 at 4117.836 kb on + strand, within HGI48_RS18345 at 4117.836 kb on + strand at 4117.874 kb on + strand, within HGI48_RS18345 at 4117.875 kb on - strand, within HGI48_RS18345 at 4117.877 kb on + strand, within HGI48_RS18345 at 4117.878 kb on - strand, within HGI48_RS18345 at 4117.950 kb on - strand, within HGI48_RS18345 at 4117.977 kb on + strand, within HGI48_RS18345 at 4117.978 kb on - strand, within HGI48_RS18345 at 4118.009 kb on + strand, within HGI48_RS18345 at 4118.009 kb on + strand, within HGI48_RS18345 at 4118.010 kb on - strand, within HGI48_RS18345 at 4118.010 kb on - strand, within HGI48_RS18345 at 4118.089 kb on - strand, within HGI48_RS18345 at 4118.089 kb on - strand, within HGI48_RS18345 at 4118.101 kb on + strand, within HGI48_RS18345 at 4118.102 kb on - strand, within HGI48_RS18345 at 4118.102 kb on - strand, within HGI48_RS18345 at 4118.102 kb on - strand, within HGI48_RS18345 at 4118.102 kb on - strand, within HGI48_RS18345 at 4118.256 kb on + strand, within HGI48_RS18345 at 4118.257 kb on - strand, within HGI48_RS18345 at 4118.461 kb on + strand, within HGI48_RS18345 at 4118.462 kb on - strand, within HGI48_RS18345 at 4118.462 kb on - strand, within HGI48_RS18345 at 4118.504 kb on + strand, within HGI48_RS18345 at 4118.504 kb on + strand, within HGI48_RS18345 at 4118.505 kb on - strand, within HGI48_RS18345 at 4118.505 kb on - strand, within HGI48_RS18345 at 4118.574 kb on - strand, within HGI48_RS18345 at 4118.668 kb on - strand at 4118.742 kb on + strand, within HGI48_RS18345 at 4118.743 kb on - strand, within HGI48_RS18345 at 4118.796 kb on + strand, within HGI48_RS18345 at 4118.864 kb on + strand, within HGI48_RS18345 at 4118.865 kb on - strand, within HGI48_RS18345 at 4118.888 kb on + strand, within HGI48_RS18345 at 4118.888 kb on + strand, within HGI48_RS18345 at 4118.940 kb on + strand, within HGI48_RS18345 at 4118.941 kb on - strand, within HGI48_RS18345 at 4118.941 kb on - strand, within HGI48_RS18345 at 4118.941 kb on - strand at 4118.942 kb on + strand, within HGI48_RS18345 at 4118.942 kb on + strand, within HGI48_RS18345 at 4118.942 kb on + strand, within HGI48_RS18345 at 4118.942 kb on + strand, within HGI48_RS18345 at 4118.942 kb on + strand at 4118.943 kb on - strand, within HGI48_RS18345 at 4118.943 kb on - strand, within HGI48_RS18345 at 4118.943 kb on - strand, within HGI48_RS18345 at 4118.943 kb on - strand, within HGI48_RS18345 at 4118.943 kb on - strand, within HGI48_RS18345 at 4119.015 kb on - strand, within HGI48_RS18345 at 4119.120 kb on + strand, within HGI48_RS18345 at 4119.120 kb on + strand, within HGI48_RS18345 at 4119.120 kb on + strand, within HGI48_RS18345 at 4119.161 kb on + strand, within HGI48_RS18345 at 4119.162 kb on - strand, within HGI48_RS18345 at 4119.162 kb on - strand, within HGI48_RS18345 at 4119.230 kb on - strand, within HGI48_RS18345 at 4119.273 kb on + strand, within HGI48_RS18345 at 4119.273 kb on + strand, within HGI48_RS18345 at 4119.273 kb on + strand, within HGI48_RS18345 at 4119.274 kb on - strand, within HGI48_RS18345 at 4119.274 kb on - strand, within HGI48_RS18345 at 4119.450 kb on + strand at 4119.450 kb on + strand at 4119.451 kb on - strand at 4119.451 kb on - strand at 4119.451 kb on - strand at 4119.471 kb on + strand at 4119.647 kb on + strand at 4119.647 kb on + strand at 4119.648 kb on - strand at 4119.648 kb on - strand at 4119.715 kb on + strand at 4119.716 kb on - strand at 4119.716 kb on - strand at 4119.735 kb on - strand at 4119.735 kb on - strand at 4119.735 kb on - strand at 4119.745 kb on + strand at 4119.746 kb on - strand at 4119.801 kb on + strand at 4119.839 kb on + strand at 4119.839 kb on + strand at 4119.839 kb on + strand at 4119.840 kb on - strand at 4119.842 kb on + strand at 4119.842 kb on + strand at 4119.843 kb on - strand at 4119.843 kb on - strand at 4119.845 kb on + strand at 4119.845 kb on + strand at 4119.883 kb on + strand at 4119.894 kb on + strand at 4119.895 kb on - strand at 4119.916 kb on + strand at 4119.919 kb on + strand at 4119.919 kb on + strand at 4119.919 kb on + strand at 4119.920 kb on - strand at 4119.920 kb on - strand at 4119.920 kb on - strand at 4119.920 kb on - strand at 4119.920 kb on - strand at 4119.940 kb on + strand at 4119.940 kb on + strand at 4119.941 kb on - strand at 4119.966 kb on + strand at 4119.970 kb on + strand at 4119.970 kb on + strand at 4119.970 kb on + strand at 4119.970 kb on + strand at 4119.971 kb on - strand at 4119.971 kb on - strand at 4119.971 kb on - strand at 4119.975 kb on + strand at 4119.975 kb on + strand at 4120.126 kb on + strand at 4120.126 kb on + strand at 4120.126 kb on + strand at 4120.126 kb on + strand at 4120.127 kb on - strand at 4120.127 kb on - strand at 4120.127 kb on - strand at 4120.135 kb on + strand at 4120.318 kb on - strand, within HGI48_RS18350 at 4120.323 kb on + strand, within HGI48_RS18350 at 4120.323 kb on + strand, within HGI48_RS18350 at 4120.323 kb on + strand, within HGI48_RS18350 at 4120.323 kb on + strand, within HGI48_RS18350 at 4120.323 kb on + strand, within HGI48_RS18350 at 4120.323 kb on + strand, within HGI48_RS18350 at 4120.323 kb on + strand, within HGI48_RS18350 at 4120.323 kb on + strand, within HGI48_RS18350 at 4120.323 kb on + strand, within HGI48_RS18350 at 4120.324 kb on - strand, within HGI48_RS18350 at 4120.324 kb on - strand, within HGI48_RS18350 at 4120.324 kb on - strand, within HGI48_RS18350 at 4120.324 kb on - strand, within HGI48_RS18350 at 4120.324 kb on - strand at 4120.360 kb on - strand, within HGI48_RS18350 at 4120.459 kb on + strand, within HGI48_RS18350 at 4120.512 kb on + strand, within HGI48_RS18350 at 4120.512 kb on + strand, within HGI48_RS18350 at 4120.512 kb on + strand, within HGI48_RS18350 at 4120.513 kb on - strand, within HGI48_RS18350 at 4120.513 kb on - strand, within HGI48_RS18350 at 4120.513 kb on - strand at 4120.513 kb on - strand, within HGI48_RS18350 at 4120.513 kb on - strand, within HGI48_RS18350 at 4120.513 kb on - strand, within HGI48_RS18350 at 4120.515 kb on + strand, within HGI48_RS18350 at 4120.515 kb on + strand, within HGI48_RS18350 at 4120.516 kb on - strand, within HGI48_RS18350 at 4120.601 kb on + strand, within HGI48_RS18350 at 4120.602 kb on - strand, within HGI48_RS18350 at 4120.602 kb on - strand, within HGI48_RS18350
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 4,116,237 + -1.7 4,116,238 - -1.3 4,116,238 - -0.1 4,116,238 - -1.4 4,116,292 + -0.7 4,116,292 + +0.1 4,116,292 + -0.4 4,116,292 + -2.8 4,116,292 + -0.7 4,116,292 + -1.2 4,116,293 - +0.7 4,116,293 - +1.1 4,116,293 - -0.1 4,116,293 - -0.3 4,116,293 - +1.6 4,116,321 + -2.0 4,116,322 - -1.2 4,116,322 - -0.9 4,116,762 + HGI48_RS18340 0.55 +2.0 4,116,762 + HGI48_RS18340 0.55 -2.7 4,116,984 + HGI48_RS18340 0.79 -0.9 4,117,048 + HGI48_RS18340 0.86 -1.2 4,117,049 - HGI48_RS18340 0.86 -1.8 4,117,167 + +2.1 4,117,167 + -1.9 4,117,209 + -0.4 4,117,210 - -1.2 4,117,292 + +0.3 4,117,295 + +0.7 4,117,296 - -1.9 4,117,304 + +1.1 4,117,409 + -2.4 4,117,409 + -2.2 4,117,409 + -1.2 4,117,409 + -1.7 4,117,414 + +0.8 4,117,422 + -0.2 4,117,422 + +1.3 4,117,512 + HGI48_RS18345 0.13 -1.4 4,117,512 + HGI48_RS18345 0.13 -0.0 4,117,512 + -0.4 4,117,513 - HGI48_RS18345 0.13 -1.4 4,117,513 - HGI48_RS18345 0.13 -1.2 4,117,566 - HGI48_RS18345 0.16 -2.8 4,117,713 + HGI48_RS18345 0.22 -3.8 4,117,738 - +1.1 4,117,826 + HGI48_RS18345 0.26 -1.5 4,117,826 + +0.1 4,117,827 - HGI48_RS18345 0.26 -2.6 4,117,827 - HGI48_RS18345 0.26 -1.4 4,117,836 + HGI48_RS18345 0.27 +0.1 4,117,836 + -0.4 4,117,874 + HGI48_RS18345 0.28 +0.3 4,117,875 - HGI48_RS18345 0.28 -2.4 4,117,877 + HGI48_RS18345 0.28 -1.4 4,117,878 - HGI48_RS18345 0.28 -1.2 4,117,950 - HGI48_RS18345 0.31 +1.3 4,117,977 + HGI48_RS18345 0.32 -3.2 4,117,978 - HGI48_RS18345 0.32 -1.9 4,118,009 + HGI48_RS18345 0.34 -1.9 4,118,009 + HGI48_RS18345 0.34 -0.6 4,118,010 - HGI48_RS18345 0.34 -0.7 4,118,010 - HGI48_RS18345 0.34 +0.3 4,118,089 - HGI48_RS18345 0.37 -1.7 4,118,089 - HGI48_RS18345 0.37 +1.1 4,118,101 + HGI48_RS18345 0.37 -2.2 4,118,102 - HGI48_RS18345 0.37 +0.4 4,118,102 - HGI48_RS18345 0.37 -2.9 4,118,102 - HGI48_RS18345 0.37 -1.5 4,118,102 - HGI48_RS18345 0.37 -1.2 4,118,256 + HGI48_RS18345 0.44 -1.0 4,118,257 - HGI48_RS18345 0.44 -2.0 4,118,461 + HGI48_RS18345 0.52 -2.9 4,118,462 - HGI48_RS18345 0.52 -0.4 4,118,462 - HGI48_RS18345 0.52 -1.3 4,118,504 + HGI48_RS18345 0.54 -1.2 4,118,504 + HGI48_RS18345 0.54 +0.1 4,118,505 - HGI48_RS18345 0.54 +1.3 4,118,505 - HGI48_RS18345 0.54 -1.2 4,118,574 - HGI48_RS18345 0.57 -0.9 4,118,668 - +0.1 4,118,742 + HGI48_RS18345 0.63 -1.2 4,118,743 - HGI48_RS18345 0.63 -1.4 4,118,796 + HGI48_RS18345 0.66 -1.7 4,118,864 + HGI48_RS18345 0.68 -0.7 4,118,865 - HGI48_RS18345 0.68 -2.4 4,118,888 + HGI48_RS18345 0.69 -2.3 4,118,888 + HGI48_RS18345 0.69 -0.4 4,118,940 + HGI48_RS18345 0.72 -0.3 4,118,941 - HGI48_RS18345 0.72 -0.7 4,118,941 - HGI48_RS18345 0.72 -1.9 4,118,941 - +1.1 4,118,942 + HGI48_RS18345 0.72 +0.4 4,118,942 + HGI48_RS18345 0.72 -1.2 4,118,942 + HGI48_RS18345 0.72 -1.5 4,118,942 + HGI48_RS18345 0.72 +1.6 4,118,942 + +0.1 4,118,943 - HGI48_RS18345 0.72 -1.2 4,118,943 - HGI48_RS18345 0.72 -2.2 4,118,943 - HGI48_RS18345 0.72 -2.0 4,118,943 - HGI48_RS18345 0.72 -1.2 4,118,943 - HGI48_RS18345 0.72 -1.7 4,119,015 - HGI48_RS18345 0.75 +0.1 4,119,120 + HGI48_RS18345 0.79 -1.4 4,119,120 + HGI48_RS18345 0.79 -0.4 4,119,120 + HGI48_RS18345 0.79 -1.2 4,119,161 + HGI48_RS18345 0.81 +0.7 4,119,162 - HGI48_RS18345 0.81 -0.1 4,119,162 - HGI48_RS18345 0.81 -1.2 4,119,230 - HGI48_RS18345 0.83 -0.4 4,119,273 + HGI48_RS18345 0.85 -1.8 4,119,273 + HGI48_RS18345 0.85 -0.1 4,119,273 + HGI48_RS18345 0.85 +2.0 4,119,274 - HGI48_RS18345 0.85 +0.1 4,119,274 - HGI48_RS18345 0.85 -0.6 4,119,450 + -0.8 4,119,450 + +0.2 4,119,451 - -0.7 4,119,451 - +0.2 4,119,451 - -0.8 4,119,471 + -1.8 4,119,647 + -1.4 4,119,647 + -0.8 4,119,648 - -1.7 4,119,648 - -2.5 4,119,715 + -0.2 4,119,716 - +0.6 4,119,716 - -0.4 4,119,735 - +0.2 4,119,735 - -1.2 4,119,735 - -2.4 4,119,745 + -2.9 4,119,746 - +0.2 4,119,801 + -2.4 4,119,839 + -1.5 4,119,839 + +0.3 4,119,839 + -2.0 4,119,840 - -0.8 4,119,842 + -0.1 4,119,842 + -0.4 4,119,843 - -0.6 4,119,843 - -0.5 4,119,845 + -2.3 4,119,845 + +0.2 4,119,883 + -0.8 4,119,894 + -1.2 4,119,895 - -2.0 4,119,916 + -0.8 4,119,919 + -2.0 4,119,919 + -0.8 4,119,919 + -0.9 4,119,920 - -1.2 4,119,920 - -1.2 4,119,920 - -1.2 4,119,920 - -1.4 4,119,920 - -0.8 4,119,940 + -0.8 4,119,940 + -1.4 4,119,941 - +1.7 4,119,966 + +0.4 4,119,970 + -0.4 4,119,970 + +0.0 4,119,970 + -0.7 4,119,970 + +1.8 4,119,971 - +0.6 4,119,971 - -2.0 4,119,971 - -1.2 4,119,975 + +0.6 4,119,975 + -1.2 4,120,126 + -1.2 4,120,126 + +0.2 4,120,126 + -1.7 4,120,126 + -2.7 4,120,127 - -1.2 4,120,127 - -1.4 4,120,127 - +1.2 4,120,135 + -1.3 4,120,318 - HGI48_RS18350 0.35 +0.7 4,120,323 + HGI48_RS18350 0.36 +0.2 4,120,323 + HGI48_RS18350 0.36 -0.0 4,120,323 + HGI48_RS18350 0.36 -1.2 4,120,323 + HGI48_RS18350 0.36 -0.8 4,120,323 + HGI48_RS18350 0.36 -0.1 4,120,323 + HGI48_RS18350 0.36 -2.0 4,120,323 + HGI48_RS18350 0.36 -1.4 4,120,323 + HGI48_RS18350 0.36 -1.4 4,120,323 + HGI48_RS18350 0.36 -0.4 4,120,324 - HGI48_RS18350 0.36 +1.3 4,120,324 - HGI48_RS18350 0.36 +0.2 4,120,324 - HGI48_RS18350 0.36 +1.1 4,120,324 - HGI48_RS18350 0.36 -0.4 4,120,324 - -0.4 4,120,360 - HGI48_RS18350 0.41 +1.0 4,120,459 + HGI48_RS18350 0.55 -0.4 4,120,512 + HGI48_RS18350 0.63 -1.7 4,120,512 + HGI48_RS18350 0.63 -1.2 4,120,512 + HGI48_RS18350 0.63 -2.8 4,120,513 - HGI48_RS18350 0.63 +2.3 4,120,513 - HGI48_RS18350 0.63 -1.2 4,120,513 - +0.6 4,120,513 - HGI48_RS18350 0.63 +0.2 4,120,513 - HGI48_RS18350 0.63 -1.7 4,120,513 - HGI48_RS18350 0.63 +0.7 4,120,515 + HGI48_RS18350 0.63 -0.0 4,120,515 + HGI48_RS18350 0.63 +2.0 4,120,516 - HGI48_RS18350 0.63 -1.0 4,120,601 + HGI48_RS18350 0.75 -1.7 4,120,602 - HGI48_RS18350 0.75 -0.4 4,120,602 - HGI48_RS18350 0.75 +1.3
Or see this region's nucleotide sequence