Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS15410 and HGI48_RS15415 overlap by 4 nucleotides HGI48_RS15415 and HGI48_RS15420 are separated by 738 nucleotides HGI48_RS15420 and HGI48_RS15425 are separated by 6 nucleotides HGI48_RS15425 and HGI48_RS15430 overlap by 23 nucleotides
HGI48_RS15410: HGI48_RS15410 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 3,469,380 to 3,470,183
_RS15410
HGI48_RS15415: HGI48_RS15415 - hydroxyethylthiazole kinase, at 3,470,180 to 3,470,287
_RS15415
HGI48_RS15420: HGI48_RS15420 - transporter substrate-binding domain-containing protein, at 3,471,026 to 3,471,784
_RS15420
HGI48_RS15425: HGI48_RS15425 - amino acid ABC transporter permease, at 3,471,791 to 3,472,492
_RS15425
HGI48_RS15430: HGI48_RS15430 - amino acid ABC transporter ATP-binding protein, at 3,472,470 to 3,473,195
_RS15430
Position (kb)
3471
3472 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3470.049 kb on - strand, within HGI48_RS15410 at 3470.111 kb on + strand at 3470.111 kb on + strand at 3470.111 kb on + strand at 3470.184 kb on - strand at 3470.298 kb on - strand at 3470.370 kb on + strand at 3470.380 kb on + strand at 3470.380 kb on + strand at 3470.394 kb on + strand at 3470.394 kb on + strand at 3470.395 kb on - strand at 3470.460 kb on + strand at 3470.500 kb on + strand at 3470.521 kb on - strand at 3470.521 kb on - strand at 3470.568 kb on - strand at 3470.576 kb on + strand at 3470.577 kb on - strand at 3470.583 kb on + strand at 3470.583 kb on + strand at 3470.647 kb on + strand at 3470.885 kb on + strand at 3470.995 kb on - strand at 3470.998 kb on + strand at 3470.999 kb on - strand at 3470.999 kb on - strand at 3471.076 kb on + strand at 3471.077 kb on - strand at 3471.150 kb on + strand, within HGI48_RS15420 at 3471.207 kb on - strand, within HGI48_RS15420 at 3471.259 kb on + strand, within HGI48_RS15420 at 3471.260 kb on - strand, within HGI48_RS15420 at 3471.260 kb on - strand, within HGI48_RS15420 at 3471.265 kb on - strand, within HGI48_RS15420 at 3471.310 kb on - strand, within HGI48_RS15420 at 3471.506 kb on + strand, within HGI48_RS15420 at 3471.576 kb on + strand, within HGI48_RS15420 at 3471.650 kb on + strand at 3471.654 kb on + strand, within HGI48_RS15420 at 3471.655 kb on - strand, within HGI48_RS15420 at 3471.666 kb on + strand, within HGI48_RS15420 at 3471.790 kb on + strand at 3471.790 kb on + strand at 3471.790 kb on + strand at 3471.791 kb on - strand at 3471.791 kb on - strand at 3471.814 kb on + strand at 3472.066 kb on + strand, within HGI48_RS15425 at 3472.125 kb on + strand, within HGI48_RS15425 at 3472.174 kb on - strand, within HGI48_RS15425 at 3472.181 kb on - strand, within HGI48_RS15425 at 3472.182 kb on + strand at 3472.282 kb on - strand, within HGI48_RS15425 at 3472.286 kb on - strand, within HGI48_RS15425 at 3472.567 kb on + strand, within HGI48_RS15430 at 3472.741 kb on - strand, within HGI48_RS15430 at 3472.741 kb on - strand, within HGI48_RS15430 at 3472.752 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,470,049 - HGI48_RS15410 0.83 -2.9 3,470,111 + -2.1 3,470,111 + -1.4 3,470,111 + -1.8 3,470,184 - -2.4 3,470,298 - -0.5 3,470,370 + -1.4 3,470,380 + -3.1 3,470,380 + +2.7 3,470,394 + -1.9 3,470,394 + +0.2 3,470,395 - -0.1 3,470,460 + -0.0 3,470,500 + -1.3 3,470,521 - +1.6 3,470,521 - -0.8 3,470,568 - -1.4 3,470,576 + -0.5 3,470,577 - -1.7 3,470,583 + +0.2 3,470,583 + -1.3 3,470,647 + -2.7 3,470,885 + -1.1 3,470,995 - +1.5 3,470,998 + +0.1 3,470,999 - +1.0 3,470,999 - -2.0 3,471,076 + -1.1 3,471,077 - -1.1 3,471,150 + HGI48_RS15420 0.16 -0.4 3,471,207 - HGI48_RS15420 0.24 -0.7 3,471,259 + HGI48_RS15420 0.31 -1.5 3,471,260 - HGI48_RS15420 0.31 +1.1 3,471,260 - HGI48_RS15420 0.31 -0.4 3,471,265 - HGI48_RS15420 0.31 -2.1 3,471,310 - HGI48_RS15420 0.37 +1.2 3,471,506 + HGI48_RS15420 0.63 +0.0 3,471,576 + HGI48_RS15420 0.72 +0.9 3,471,650 + -0.7 3,471,654 + HGI48_RS15420 0.83 -1.2 3,471,655 - HGI48_RS15420 0.83 +2.4 3,471,666 + HGI48_RS15420 0.84 +0.3 3,471,790 + -1.0 3,471,790 + +0.6 3,471,790 + -0.1 3,471,791 - -0.5 3,471,791 - -0.0 3,471,814 + -0.5 3,472,066 + HGI48_RS15425 0.39 -0.1 3,472,125 + HGI48_RS15425 0.48 -1.9 3,472,174 - HGI48_RS15425 0.55 -2.1 3,472,181 - HGI48_RS15425 0.56 -0.6 3,472,182 + +0.3 3,472,282 - HGI48_RS15425 0.70 -0.8 3,472,286 - HGI48_RS15425 0.71 -0.6 3,472,567 + HGI48_RS15430 0.13 +0.3 3,472,741 - HGI48_RS15430 0.37 -1.1 3,472,741 - HGI48_RS15430 0.37 -1.7 3,472,752 - +0.3
Or see this region's nucleotide sequence