Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS05705 and HGI48_RS05710 are separated by 138 nucleotides HGI48_RS05710 and HGI48_RS05715 are separated by 112 nucleotides HGI48_RS05715 and HGI48_RS05720 are separated by 126 nucleotides
HGI48_RS05705: HGI48_RS05705 - peptidylprolyl isomerase, at 1,203,361 to 1,205,241
_RS05705
HGI48_RS05710: HGI48_RS05710 - helix-hairpin-helix domain-containing protein, at 1,205,380 to 1,205,754
_RS05710
HGI48_RS05715: HGI48_RS05715 - 7-cyano-7-deazaguanine synthase QueC, at 1,205,867 to 1,206,562
_RS05715
HGI48_RS05720: HGI48_RS05720 - PLP-dependent cysteine synthase family protein, at 1,206,689 to 1,207,741
_RS05720
Position (kb)
1205
1206 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1204.490 kb on + strand, within HGI48_RS05705 at 1204.490 kb on + strand at 1204.491 kb on - strand, within HGI48_RS05705 at 1204.491 kb on - strand, within HGI48_RS05705 at 1204.519 kb on + strand, within HGI48_RS05705 at 1204.719 kb on + strand at 1204.719 kb on + strand, within HGI48_RS05705 at 1204.720 kb on - strand, within HGI48_RS05705 at 1204.720 kb on - strand, within HGI48_RS05705 at 1204.720 kb on - strand, within HGI48_RS05705 at 1204.720 kb on - strand, within HGI48_RS05705 at 1204.897 kb on + strand at 1204.898 kb on - strand, within HGI48_RS05705 at 1205.070 kb on - strand at 1205.212 kb on + strand at 1205.239 kb on + strand at 1205.240 kb on - strand at 1205.406 kb on - strand at 1205.406 kb on - strand at 1205.481 kb on + strand, within HGI48_RS05710 at 1205.571 kb on + strand at 1205.571 kb on + strand, within HGI48_RS05710 at 1205.572 kb on - strand, within HGI48_RS05710 at 1205.574 kb on + strand, within HGI48_RS05710 at 1205.575 kb on - strand, within HGI48_RS05710 at 1205.575 kb on - strand, within HGI48_RS05710 at 1205.575 kb on - strand, within HGI48_RS05710 at 1205.752 kb on + strand at 1205.752 kb on + strand at 1205.829 kb on + strand at 1205.829 kb on + strand at 1205.926 kb on - strand at 1205.926 kb on - strand at 1205.997 kb on + strand, within HGI48_RS05715 at 1206.010 kb on + strand, within HGI48_RS05715 at 1206.010 kb on + strand, within HGI48_RS05715 at 1206.040 kb on - strand, within HGI48_RS05715 at 1206.042 kb on + strand, within HGI48_RS05715 at 1206.043 kb on - strand, within HGI48_RS05715 at 1206.172 kb on - strand, within HGI48_RS05715 at 1206.221 kb on + strand, within HGI48_RS05715 at 1206.222 kb on - strand, within HGI48_RS05715 at 1206.234 kb on + strand, within HGI48_RS05715 at 1206.234 kb on + strand, within HGI48_RS05715 at 1206.235 kb on - strand, within HGI48_RS05715 at 1206.235 kb on - strand, within HGI48_RS05715 at 1206.284 kb on - strand, within HGI48_RS05715 at 1206.346 kb on + strand, within HGI48_RS05715 at 1206.460 kb on - strand, within HGI48_RS05715 at 1206.490 kb on - strand at 1206.616 kb on + strand at 1206.616 kb on + strand at 1206.616 kb on + strand at 1206.616 kb on + strand at 1206.617 kb on - strand at 1206.617 kb on - strand at 1206.634 kb on - strand at 1206.734 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 1,204,490 + HGI48_RS05705 0.60 +0.3 1,204,490 + +0.4 1,204,491 - HGI48_RS05705 0.60 +0.5 1,204,491 - HGI48_RS05705 0.60 -0.4 1,204,519 + HGI48_RS05705 0.62 -0.1 1,204,719 + -0.6 1,204,719 + HGI48_RS05705 0.72 -1.4 1,204,720 - HGI48_RS05705 0.72 +0.5 1,204,720 - HGI48_RS05705 0.72 +0.6 1,204,720 - HGI48_RS05705 0.72 -0.9 1,204,720 - HGI48_RS05705 0.72 -0.5 1,204,897 + +2.7 1,204,898 - HGI48_RS05705 0.82 -0.5 1,205,070 - +0.6 1,205,212 + +1.7 1,205,239 + -1.6 1,205,240 - +0.7 1,205,406 - -0.1 1,205,406 - -1.4 1,205,481 + HGI48_RS05710 0.27 -0.4 1,205,571 + -0.6 1,205,571 + HGI48_RS05710 0.51 -0.8 1,205,572 - HGI48_RS05710 0.51 +0.0 1,205,574 + HGI48_RS05710 0.52 +0.1 1,205,575 - HGI48_RS05710 0.52 +0.0 1,205,575 - HGI48_RS05710 0.52 -1.6 1,205,575 - HGI48_RS05710 0.52 +0.9 1,205,752 + -0.1 1,205,752 + -0.4 1,205,829 + +1.2 1,205,829 + +0.5 1,205,926 - +0.3 1,205,926 - +0.5 1,205,997 + HGI48_RS05715 0.19 -1.1 1,206,010 + HGI48_RS05715 0.21 +1.4 1,206,010 + HGI48_RS05715 0.21 -0.6 1,206,040 - HGI48_RS05715 0.25 +0.7 1,206,042 + HGI48_RS05715 0.25 -0.6 1,206,043 - HGI48_RS05715 0.25 -0.1 1,206,172 - HGI48_RS05715 0.44 -0.3 1,206,221 + HGI48_RS05715 0.51 +0.0 1,206,222 - HGI48_RS05715 0.51 -0.6 1,206,234 + HGI48_RS05715 0.53 -0.4 1,206,234 + HGI48_RS05715 0.53 -2.2 1,206,235 - HGI48_RS05715 0.53 -0.1 1,206,235 - HGI48_RS05715 0.53 -0.8 1,206,284 - HGI48_RS05715 0.60 -0.2 1,206,346 + HGI48_RS05715 0.69 -1.9 1,206,460 - HGI48_RS05715 0.85 -0.5 1,206,490 - +0.9 1,206,616 + +0.3 1,206,616 + -0.6 1,206,616 + -0.5 1,206,616 + -0.1 1,206,617 - -0.8 1,206,617 - -0.3 1,206,634 - +1.3 1,206,734 - +0.3
Or see this region's nucleotide sequence