Strain Fitness in Dickeya dianthicola 67-19 around HGI48_RS05190

Experiment: M9+glycerol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHGI48_RS05180 and HGI48_RS05185 overlap by 4 nucleotidesHGI48_RS05185 and HGI48_RS05190 are separated by 75 nucleotidesHGI48_RS05190 and HGI48_RS05195 are separated by 12 nucleotides HGI48_RS05180: HGI48_RS05180 - lipid-A-disaccharide synthase, at 1,085,793 to 1,086,941 _RS05180 HGI48_RS05185: HGI48_RS05185 - ribonuclease HII, at 1,086,938 to 1,087,534 _RS05185 HGI48_RS05190: HGI48_RS05190 - DNA polymerase III subunit alpha, at 1,087,610 to 1,091,092 _RS05190 HGI48_RS05195: HGI48_RS05195 - acetyl-CoA carboxylase carboxyl transferase subunit alpha, at 1,091,105 to 1,092,064 _RS05195 Position (kb) 1087 1088 1089 1090 1091 1092Strain fitness (log2 ratio) -2 -1 0 1 2at 1086.973 kb on + strandat 1086.974 kb on - strandat 1086.974 kb on - strandat 1086.974 kb on - strandat 1086.974 kb on - strandat 1086.993 kb on + strandat 1086.993 kb on + strandat 1086.994 kb on - strandat 1087.184 kb on - strandat 1087.445 kb on + strand, within HGI48_RS05185at 1087.445 kb on + strandat 1087.445 kb on + strandat 1087.537 kb on + strandat 1087.554 kb on + strandat 1087.554 kb on + strandat 1087.554 kb on + strandat 1087.554 kb on + strandat 1087.554 kb on + strandat 1087.554 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9+glycerol
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1,086,973 + -1.0
1,086,974 - +1.2
1,086,974 - +0.2
1,086,974 - +0.6
1,086,974 - -1.6
1,086,993 + -0.8
1,086,993 + +1.0
1,086,994 - -0.3
1,087,184 - +1.0
1,087,445 + HGI48_RS05185 0.85 -2.0
1,087,445 + +2.6
1,087,445 + -0.6
1,087,537 + -1.3
1,087,554 + -1.8
1,087,554 + -2.6
1,087,554 + -2.3
1,087,554 + +0.7
1,087,554 + +1.0
1,087,554 + -2.5

Or see this region's nucleotide sequence