Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS04140 and HGI48_RS04145 are separated by 66 nucleotides HGI48_RS04145 and HGI48_RS04150 are separated by 188 nucleotides
HGI48_RS04140: HGI48_RS04140 - IS3 family transposase, at 875,660 to 876,811
_RS04140
HGI48_RS04145: HGI48_RS04145 - carboxymuconolactone decarboxylase family protein, at 876,878 to 877,060
_RS04145
HGI48_RS04150: HGI48_RS04150 - alkene reductase, at 877,249 to 878,361
_RS04150
Position (kb) 876
877
878 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 877.000 kb on + strand, within HGI48_RS04145 at 877.074 kb on + strand at 877.116 kb on + strand at 877.144 kb on - strand at 877.375 kb on + strand, within HGI48_RS04150 at 877.375 kb on + strand, within HGI48_RS04150 at 877.376 kb on - strand at 877.376 kb on - strand, within HGI48_RS04150 at 877.409 kb on - strand at 877.549 kb on - strand, within HGI48_RS04150 at 877.600 kb on - strand, within HGI48_RS04150 at 877.602 kb on - strand at 877.638 kb on + strand, within HGI48_RS04150 at 877.639 kb on - strand, within HGI48_RS04150 at 877.639 kb on - strand, within HGI48_RS04150 at 877.754 kb on + strand at 877.755 kb on - strand, within HGI48_RS04150 at 877.755 kb on - strand at 877.757 kb on - strand, within HGI48_RS04150 at 877.757 kb on - strand at 877.758 kb on + strand, within HGI48_RS04150 at 877.758 kb on + strand, within HGI48_RS04150 at 877.759 kb on - strand, within HGI48_RS04150 at 877.767 kb on + strand, within HGI48_RS04150 at 877.767 kb on + strand, within HGI48_RS04150 at 877.768 kb on - strand at 877.773 kb on + strand, within HGI48_RS04150 at 877.805 kb on + strand, within HGI48_RS04150 at 877.945 kb on + strand, within HGI48_RS04150 at 877.946 kb on - strand, within HGI48_RS04150 at 877.997 kb on - strand, within HGI48_RS04150 at 878.004 kb on + strand at 878.004 kb on + strand, within HGI48_RS04150 at 878.004 kb on + strand, within HGI48_RS04150 at 878.037 kb on + strand, within HGI48_RS04150 at 878.037 kb on + strand, within HGI48_RS04150 at 878.037 kb on + strand at 878.037 kb on + strand, within HGI48_RS04150
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 877,000 + HGI48_RS04145 0.67 +0.9 877,074 + -1.3 877,116 + -1.8 877,144 - -0.5 877,375 + HGI48_RS04150 0.11 -1.1 877,375 + HGI48_RS04150 0.11 -0.3 877,376 - +1.8 877,376 - HGI48_RS04150 0.11 +0.0 877,409 - +2.0 877,549 - HGI48_RS04150 0.27 +0.1 877,600 - HGI48_RS04150 0.32 -1.1 877,602 - -0.5 877,638 + HGI48_RS04150 0.35 -0.5 877,639 - HGI48_RS04150 0.35 -0.5 877,639 - HGI48_RS04150 0.35 -1.1 877,754 + +0.0 877,755 - HGI48_RS04150 0.45 -3.1 877,755 - +0.5 877,757 - HGI48_RS04150 0.46 +0.5 877,757 - -0.5 877,758 + HGI48_RS04150 0.46 -1.5 877,758 + HGI48_RS04150 0.46 +0.7 877,759 - HGI48_RS04150 0.46 -1.1 877,767 + HGI48_RS04150 0.47 -1.0 877,767 + HGI48_RS04150 0.47 -3.7 877,768 - +1.8 877,773 + HGI48_RS04150 0.47 +1.0 877,805 + HGI48_RS04150 0.50 +1.3 877,945 + HGI48_RS04150 0.63 +1.0 877,946 - HGI48_RS04150 0.63 +0.2 877,997 - HGI48_RS04150 0.67 -1.8 878,004 + -0.5 878,004 + HGI48_RS04150 0.68 -2.1 878,004 + HGI48_RS04150 0.68 -2.3 878,037 + HGI48_RS04150 0.71 -0.8 878,037 + HGI48_RS04150 0.71 -1.8 878,037 + +2.0 878,037 + HGI48_RS04150 0.71 +0.0
Or see this region's nucleotide sequence