Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS03385 and HGI48_RS03390 are separated by 814 nucleotides HGI48_RS03390 and HGI48_RS03395 are separated by 30 nucleotides HGI48_RS03395 and HGI48_RS03400 are separated by 131 nucleotides
HGI48_RS03385: HGI48_RS03385 - acetolactate synthase AlsS, at 709,496 to 711,175
_RS03385
HGI48_RS03390: HGI48_RS03390 - glycine cleavage system aminomethyltransferase GcvT, at 711,990 to 713,090
_RS03390
HGI48_RS03395: HGI48_RS03395 - glycine cleavage system protein GcvH, at 713,121 to 713,528
_RS03395
HGI48_RS03400: HGI48_RS03400 - aminomethyl-transferring glycine dehydrogenase, at 713,660 to 716,533
_RS03400
Position (kb) 711
712
713
714 Strain fitness (log2 ratio) -2
-1
0
1 at 711.005 kb on + strand, within HGI48_RS03385 at 711.123 kb on - strand at 711.179 kb on + strand at 711.179 kb on + strand at 711.179 kb on + strand at 711.180 kb on - strand at 711.180 kb on - strand at 711.180 kb on - strand at 711.182 kb on + strand at 711.182 kb on + strand at 711.183 kb on - strand at 711.183 kb on - strand at 711.200 kb on + strand at 711.200 kb on + strand at 711.207 kb on + strand at 711.258 kb on + strand at 711.259 kb on - strand at 711.259 kb on - strand at 711.259 kb on - strand at 711.268 kb on + strand at 711.268 kb on + strand at 711.293 kb on + strand at 711.424 kb on + strand at 711.454 kb on - strand at 711.474 kb on + strand at 711.474 kb on + strand at 711.474 kb on + strand at 711.475 kb on - strand at 711.475 kb on - strand at 711.486 kb on + strand at 711.487 kb on - strand at 711.487 kb on - strand at 711.487 kb on - strand at 711.539 kb on + strand at 711.539 kb on + strand at 711.539 kb on + strand at 711.539 kb on + strand at 711.540 kb on - strand at 711.540 kb on - strand at 711.540 kb on - strand at 711.864 kb on - strand at 711.914 kb on + strand at 711.977 kb on + strand at 711.978 kb on - strand at 711.978 kb on - strand at 711.978 kb on - strand at 712.011 kb on + strand at 712.012 kb on - strand at 712.012 kb on - strand at 712.012 kb on - strand at 712.012 kb on - strand at 712.078 kb on - strand at 712.191 kb on + strand, within HGI48_RS03390 at 712.242 kb on + strand, within HGI48_RS03390 at 712.243 kb on - strand at 712.243 kb on - strand, within HGI48_RS03390 at 712.243 kb on - strand, within HGI48_RS03390 at 712.276 kb on + strand, within HGI48_RS03390 at 712.277 kb on - strand, within HGI48_RS03390 at 712.277 kb on - strand at 712.294 kb on - strand, within HGI48_RS03390 at 712.312 kb on - strand, within HGI48_RS03390 at 712.312 kb on - strand, within HGI48_RS03390 at 712.386 kb on - strand, within HGI48_RS03390 at 712.386 kb on - strand, within HGI48_RS03390 at 712.386 kb on - strand, within HGI48_RS03390 at 712.386 kb on - strand, within HGI48_RS03390 at 712.554 kb on + strand, within HGI48_RS03390 at 712.555 kb on - strand, within HGI48_RS03390 at 712.555 kb on - strand, within HGI48_RS03390 at 712.555 kb on - strand, within HGI48_RS03390 at 712.555 kb on - strand, within HGI48_RS03390 at 712.556 kb on + strand, within HGI48_RS03390 at 712.556 kb on + strand, within HGI48_RS03390 at 712.557 kb on - strand, within HGI48_RS03390 at 712.696 kb on - strand, within HGI48_RS03390 at 713.148 kb on + strand at 713.148 kb on + strand at 713.148 kb on + strand at 713.148 kb on + strand at 713.148 kb on + strand at 713.148 kb on + strand at 713.149 kb on - strand at 713.149 kb on - strand at 713.149 kb on - strand at 713.149 kb on - strand at 713.150 kb on + strand at 713.150 kb on + strand at 713.150 kb on + strand at 713.150 kb on + strand at 713.150 kb on + strand at 713.151 kb on - strand at 713.151 kb on - strand at 713.193 kb on + strand, within HGI48_RS03395 at 713.247 kb on - strand, within HGI48_RS03395 at 713.331 kb on + strand, within HGI48_RS03395 at 713.331 kb on + strand, within HGI48_RS03395 at 713.331 kb on + strand, within HGI48_RS03395 at 713.331 kb on + strand, within HGI48_RS03395 at 713.332 kb on - strand, within HGI48_RS03395 at 713.332 kb on - strand, within HGI48_RS03395 at 713.332 kb on - strand, within HGI48_RS03395 at 713.332 kb on - strand, within HGI48_RS03395 at 713.526 kb on + strand at 713.527 kb on - strand at 713.527 kb on - strand at 713.566 kb on + strand at 713.566 kb on + strand at 713.566 kb on + strand at 713.566 kb on + strand at 713.566 kb on + strand at 713.567 kb on - strand at 713.638 kb on + strand at 713.639 kb on - strand at 713.639 kb on - strand at 713.909 kb on + strand at 713.909 kb on + strand at 713.909 kb on + strand at 713.910 kb on - strand at 713.910 kb on - strand at 713.984 kb on + strand, within HGI48_RS03400 at 713.984 kb on + strand at 713.985 kb on - strand, within HGI48_RS03400 at 713.985 kb on - strand, within HGI48_RS03400 at 713.987 kb on - strand, within HGI48_RS03400 at 713.993 kb on + strand at 713.995 kb on + strand, within HGI48_RS03400 at 713.996 kb on - strand, within HGI48_RS03400
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 711,005 + HGI48_RS03385 0.90 -1.2 711,123 - -0.5 711,179 + -0.5 711,179 + +1.7 711,179 + -1.5 711,180 - -0.1 711,180 - +0.1 711,180 - +1.6 711,182 + -1.5 711,182 + +0.6 711,183 - -0.1 711,183 - -1.9 711,200 + -0.5 711,200 + -0.3 711,207 + -0.4 711,258 + -0.7 711,259 - -0.5 711,259 - +0.4 711,259 - -0.8 711,268 + -0.1 711,268 + +1.1 711,293 + -1.5 711,424 + -0.0 711,454 - -0.2 711,474 + -0.2 711,474 + -1.2 711,474 + -1.9 711,475 - +0.3 711,475 - -0.2 711,486 + -1.2 711,487 - +0.1 711,487 - -1.5 711,487 - -0.6 711,539 + -1.9 711,539 + -0.9 711,539 + +0.9 711,539 + -1.2 711,540 - -0.9 711,540 - -1.1 711,540 - -1.7 711,864 - +0.4 711,914 + -1.1 711,977 + +0.5 711,978 - -0.5 711,978 - -0.9 711,978 - +1.1 712,011 + -0.9 712,012 - +0.1 712,012 - +1.7 712,012 - -0.5 712,012 - -1.2 712,078 - +0.1 712,191 + HGI48_RS03390 0.18 -0.6 712,242 + HGI48_RS03390 0.23 -2.5 712,243 - +0.5 712,243 - HGI48_RS03390 0.23 -1.2 712,243 - HGI48_RS03390 0.23 -2.4 712,276 + HGI48_RS03390 0.26 -2.6 712,277 - HGI48_RS03390 0.26 -1.2 712,277 - +0.5 712,294 - HGI48_RS03390 0.28 -0.8 712,312 - HGI48_RS03390 0.29 -1.2 712,312 - HGI48_RS03390 0.29 +0.6 712,386 - HGI48_RS03390 0.36 -0.5 712,386 - HGI48_RS03390 0.36 -0.8 712,386 - HGI48_RS03390 0.36 -1.2 712,386 - HGI48_RS03390 0.36 -0.9 712,554 + HGI48_RS03390 0.51 -1.4 712,555 - HGI48_RS03390 0.51 -0.5 712,555 - HGI48_RS03390 0.51 -1.2 712,555 - HGI48_RS03390 0.51 -0.9 712,555 - HGI48_RS03390 0.51 -0.4 712,556 + HGI48_RS03390 0.51 -1.8 712,556 + HGI48_RS03390 0.51 +1.4 712,557 - HGI48_RS03390 0.51 -1.5 712,696 - HGI48_RS03390 0.64 -2.6 713,148 + +0.3 713,148 + +0.1 713,148 + -1.9 713,148 + -1.5 713,148 + -1.0 713,148 + -1.5 713,149 - -0.5 713,149 - -1.7 713,149 - +0.5 713,149 - -1.2 713,150 + -0.2 713,150 + -1.2 713,150 + -2.1 713,150 + +1.1 713,150 + -0.5 713,151 - +0.1 713,151 - +0.1 713,193 + HGI48_RS03395 0.18 +0.4 713,247 - HGI48_RS03395 0.31 +1.1 713,331 + HGI48_RS03395 0.51 -0.4 713,331 + HGI48_RS03395 0.51 -0.6 713,331 + HGI48_RS03395 0.51 -0.9 713,331 + HGI48_RS03395 0.51 -0.7 713,332 - HGI48_RS03395 0.52 -1.6 713,332 - HGI48_RS03395 0.52 -1.9 713,332 - HGI48_RS03395 0.52 -1.5 713,332 - HGI48_RS03395 0.52 -2.6 713,526 + -1.5 713,527 - -1.6 713,527 - -2.1 713,566 + -0.2 713,566 + -1.5 713,566 + +0.1 713,566 + +1.6 713,566 + +1.1 713,567 - -2.2 713,638 + +0.4 713,639 - -2.3 713,639 - +0.1 713,909 + +1.5 713,909 + -0.3 713,909 + +0.6 713,910 - -0.4 713,910 - -0.7 713,984 + HGI48_RS03400 0.11 +0.4 713,984 + -0.5 713,985 - HGI48_RS03400 0.11 +1.7 713,985 - HGI48_RS03400 0.11 -2.7 713,987 - HGI48_RS03400 0.11 +0.8 713,993 + +1.5 713,995 + HGI48_RS03400 0.12 -0.0 713,996 - HGI48_RS03400 0.12 -1.7
Or see this region's nucleotide sequence