Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS03235 and HGI48_RS03240 are separated by 25 nucleotides HGI48_RS03240 and HGI48_RS03245 are separated by 74 nucleotides HGI48_RS03245 and HGI48_RS03250 overlap by 1 nucleotides
HGI48_RS03235: HGI48_RS03235 - transcription termination/antitermination protein NusA, at 681,671 to 683,161
_RS03235
HGI48_RS03240: HGI48_RS03240 - translation initiation factor IF-2, at 683,187 to 685,904
_RS03240
HGI48_RS03245: HGI48_RS03245 - 30S ribosome-binding factor RbfA, at 685,979 to 686,380
_RS03245
HGI48_RS03250: HGI48_RS03250 - tRNA pseudouridine(55) synthase TruB, at 686,380 to 687,336
_RS03250
Position (kb) 683
684
685
686 Strain fitness (log2 ratio) -1
0
1
2 at 683.008 kb on + strand, within HGI48_RS03235 at 683.131 kb on + strand at 683.131 kb on + strand at 683.131 kb on + strand at 683.169 kb on + strand at 683.169 kb on + strand at 686.347 kb on + strand at 686.379 kb on - strand at 686.474 kb on + strand at 686.475 kb on - strand at 686.508 kb on + strand, within HGI48_RS03250 at 686.509 kb on - strand, within HGI48_RS03250 at 686.509 kb on - strand, within HGI48_RS03250 at 686.509 kb on - strand, within HGI48_RS03250 at 686.598 kb on + strand, within HGI48_RS03250 at 686.598 kb on + strand at 686.640 kb on + strand, within HGI48_RS03250 at 686.658 kb on + strand, within HGI48_RS03250 at 686.658 kb on + strand at 686.658 kb on + strand, within HGI48_RS03250 at 686.658 kb on + strand, within HGI48_RS03250 at 686.756 kb on - strand at 686.756 kb on - strand, within HGI48_RS03250 at 686.756 kb on - strand, within HGI48_RS03250 at 686.756 kb on - strand, within HGI48_RS03250 at 686.770 kb on + strand, within HGI48_RS03250 at 686.846 kb on - strand, within HGI48_RS03250 at 686.853 kb on - strand, within HGI48_RS03250 at 686.853 kb on - strand, within HGI48_RS03250
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 683,008 + HGI48_RS03235 0.90 +0.7 683,131 + +1.3 683,131 + -1.2 683,131 + -0.6 683,169 + -1.2 683,169 + -0.9 686,347 + -1.2 686,379 - -1.5 686,474 + +0.7 686,475 - -1.5 686,508 + HGI48_RS03250 0.13 -1.2 686,509 - HGI48_RS03250 0.13 +1.0 686,509 - HGI48_RS03250 0.13 +0.4 686,509 - HGI48_RS03250 0.13 -0.8 686,598 + HGI48_RS03250 0.23 -0.9 686,598 + +2.3 686,640 + HGI48_RS03250 0.27 +0.1 686,658 + HGI48_RS03250 0.29 -0.9 686,658 + +0.5 686,658 + HGI48_RS03250 0.29 -0.2 686,658 + HGI48_RS03250 0.29 -0.9 686,756 - +1.1 686,756 - HGI48_RS03250 0.39 -1.3 686,756 - HGI48_RS03250 0.39 +0.1 686,756 - HGI48_RS03250 0.39 -0.9 686,770 + HGI48_RS03250 0.41 -1.5 686,846 - HGI48_RS03250 0.49 +0.5 686,853 - HGI48_RS03250 0.49 +0.8 686,853 - HGI48_RS03250 0.49 +0.5
Or see this region's nucleotide sequence