Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS03185 and HGI48_RS03190 are separated by 123 nucleotides HGI48_RS03190 and HGI48_RS03195 are separated by 158 nucleotides HGI48_RS03195 and HGI48_RS03200 are separated by 47 nucleotides
HGI48_RS03185: HGI48_RS03185 - transcription elongation factor GreA, at 674,101 to 674,577
_RS03185
HGI48_RS03190: HGI48_RS03190 - ribosome assembly RNA-binding protein YhbY, at 674,701 to 674,994
_RS03190
HGI48_RS03195: HGI48_RS03195 - 23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE, at 675,153 to 675,782
_RS03195
HGI48_RS03200: HGI48_RS03200 - ATP-dependent zinc metalloprotease FtsH, at 675,830 to 677,782
_RS03200
Position (kb) 675
676 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 674.242 kb on + strand at 674.243 kb on - strand, within HGI48_RS03185 at 674.332 kb on - strand, within HGI48_RS03185 at 674.414 kb on - strand, within HGI48_RS03185 at 674.418 kb on + strand, within HGI48_RS03185 at 674.419 kb on - strand, within HGI48_RS03185 at 674.419 kb on - strand, within HGI48_RS03185 at 674.457 kb on + strand, within HGI48_RS03185 at 674.457 kb on + strand, within HGI48_RS03185 at 674.457 kb on + strand, within HGI48_RS03185 at 674.458 kb on - strand, within HGI48_RS03185 at 674.493 kb on - strand, within HGI48_RS03185 at 674.543 kb on + strand at 674.543 kb on + strand at 674.544 kb on - strand at 674.544 kb on - strand at 674.544 kb on - strand at 674.569 kb on + strand at 674.569 kb on + strand at 674.570 kb on - strand at 674.610 kb on + strand at 674.610 kb on + strand at 674.610 kb on + strand at 674.741 kb on + strand, within HGI48_RS03190 at 674.893 kb on + strand at 674.893 kb on + strand, within HGI48_RS03190 at 674.893 kb on + strand, within HGI48_RS03190 at 674.894 kb on - strand, within HGI48_RS03190 at 674.894 kb on - strand, within HGI48_RS03190 at 674.894 kb on - strand, within HGI48_RS03190 at 674.928 kb on + strand, within HGI48_RS03190 at 674.929 kb on - strand, within HGI48_RS03190 at 675.113 kb on + strand at 675.113 kb on + strand at 675.113 kb on + strand at 675.113 kb on + strand at 675.113 kb on + strand at 675.113 kb on + strand at 675.113 kb on + strand at 675.113 kb on + strand at 675.113 kb on + strand at 675.113 kb on + strand at 675.114 kb on - strand at 675.117 kb on + strand at 675.117 kb on + strand at 675.117 kb on + strand at 675.123 kb on + strand at 675.123 kb on + strand at 675.123 kb on + strand at 675.123 kb on + strand at 675.123 kb on + strand at 675.124 kb on - strand at 675.780 kb on + strand at 675.780 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 674,242 + +1.1 674,243 - HGI48_RS03185 0.30 -1.5 674,332 - HGI48_RS03185 0.48 +0.1 674,414 - HGI48_RS03185 0.66 -1.7 674,418 + HGI48_RS03185 0.66 -0.9 674,419 - HGI48_RS03185 0.67 -1.2 674,419 - HGI48_RS03185 0.67 -0.6 674,457 + HGI48_RS03185 0.75 -0.3 674,457 + HGI48_RS03185 0.75 +0.4 674,457 + HGI48_RS03185 0.75 +1.3 674,458 - HGI48_RS03185 0.75 -1.5 674,493 - HGI48_RS03185 0.82 -0.5 674,543 + +0.8 674,543 + -0.9 674,544 - -0.5 674,544 - +0.5 674,544 - -2.2 674,569 + +0.9 674,569 + -0.6 674,570 - +0.2 674,610 + -1.7 674,610 + -0.8 674,610 + -1.2 674,741 + HGI48_RS03190 0.14 -0.8 674,893 + -0.5 674,893 + HGI48_RS03190 0.65 -0.9 674,893 + HGI48_RS03190 0.65 -2.3 674,894 - HGI48_RS03190 0.66 -1.5 674,894 - HGI48_RS03190 0.66 +0.4 674,894 - HGI48_RS03190 0.66 -0.7 674,928 + HGI48_RS03190 0.77 +1.4 674,929 - HGI48_RS03190 0.78 -0.8 675,113 + -0.9 675,113 + +0.0 675,113 + +2.7 675,113 + -0.3 675,113 + -1.5 675,113 + +0.5 675,113 + -0.3 675,113 + -0.5 675,113 + -1.9 675,113 + -0.3 675,114 - -1.7 675,117 + -0.9 675,117 + -1.5 675,117 + -3.4 675,123 + +2.1 675,123 + +1.7 675,123 + +1.1 675,123 + -0.9 675,123 + +0.5 675,124 - -0.9 675,780 + -1.5 675,780 + -0.9
Or see this region's nucleotide sequence