Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS02355 and HGI48_RS02360 are separated by 234 nucleotides HGI48_RS02360 and HGI48_RS02370 are separated by 338 nucleotides HGI48_RS02370 and HGI48_RS02375 are separated by 13 nucleotides
HGI48_RS02355: HGI48_RS02355 - YecA family protein, at 512,088 to 512,672
_RS02355
HGI48_RS02360: HGI48_RS02360 - cell division protein ZapA, at 512,907 to 513,236
_RS02360
HGI48_RS02370: HGI48_RS02370 - 5-formyltetrahydrofolate cyclo-ligase, at 513,575 to 514,189
_RS02370
HGI48_RS02375: HGI48_RS02375 - multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR, at 514,203 to 515,459
_RS02375
Position (kb) 513
514
515 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 512.626 kb on - strand at 512.657 kb on + strand at 512.657 kb on + strand at 512.657 kb on + strand at 512.658 kb on - strand at 512.658 kb on - strand at 512.658 kb on - strand at 512.664 kb on + strand at 512.667 kb on - strand at 512.707 kb on - strand at 512.711 kb on + strand at 512.730 kb on + strand at 512.731 kb on - strand at 512.731 kb on - strand at 512.732 kb on + strand at 512.770 kb on + strand at 512.770 kb on + strand at 512.770 kb on + strand at 512.770 kb on + strand at 512.770 kb on + strand at 512.771 kb on - strand at 512.771 kb on - strand at 512.777 kb on + strand at 512.777 kb on + strand at 512.777 kb on + strand at 512.778 kb on - strand at 512.778 kb on - strand at 512.778 kb on - strand at 512.778 kb on - strand at 512.788 kb on + strand at 512.788 kb on + strand at 512.788 kb on + strand at 512.788 kb on + strand at 512.788 kb on + strand at 512.788 kb on + strand at 512.789 kb on - strand at 512.789 kb on - strand at 512.789 kb on - strand at 512.795 kb on + strand at 512.795 kb on + strand at 512.813 kb on + strand at 512.813 kb on + strand at 512.813 kb on + strand at 512.813 kb on + strand at 512.813 kb on + strand at 512.815 kb on + strand at 512.815 kb on + strand at 512.815 kb on + strand at 512.820 kb on + strand at 513.235 kb on - strand at 513.296 kb on + strand at 513.296 kb on + strand at 513.296 kb on + strand at 513.297 kb on - strand at 513.297 kb on - strand at 513.297 kb on - strand at 513.297 kb on - strand at 513.297 kb on - strand at 513.297 kb on - strand at 513.297 kb on - strand at 513.301 kb on + strand at 513.302 kb on - strand at 513.302 kb on - strand at 513.381 kb on + strand at 513.381 kb on + strand at 513.382 kb on - strand at 513.382 kb on - strand at 513.382 kb on - strand at 513.428 kb on - strand at 513.531 kb on + strand at 513.531 kb on + strand at 513.531 kb on + strand at 513.544 kb on + strand at 513.561 kb on - strand at 513.621 kb on + strand at 513.830 kb on + strand, within HGI48_RS02370 at 513.830 kb on + strand, within HGI48_RS02370 at 513.831 kb on - strand, within HGI48_RS02370 at 513.831 kb on - strand, within HGI48_RS02370 at 513.876 kb on + strand, within HGI48_RS02370 at 513.877 kb on - strand, within HGI48_RS02370 at 513.877 kb on - strand, within HGI48_RS02370 at 514.041 kb on - strand, within HGI48_RS02370 at 514.041 kb on - strand, within HGI48_RS02370 at 514.193 kb on - strand at 514.289 kb on - strand at 514.289 kb on - strand at 514.312 kb on + strand at 514.312 kb on + strand at 514.312 kb on + strand at 514.312 kb on + strand at 514.312 kb on + strand at 514.312 kb on + strand at 514.313 kb on - strand at 514.313 kb on - strand at 514.313 kb on - strand at 514.317 kb on + strand at 514.318 kb on - strand at 514.337 kb on + strand, within HGI48_RS02375 at 514.337 kb on + strand, within HGI48_RS02375 at 514.337 kb on + strand, within HGI48_RS02375 at 514.337 kb on + strand, within HGI48_RS02375 at 514.337 kb on + strand, within HGI48_RS02375 at 514.338 kb on - strand, within HGI48_RS02375 at 514.338 kb on - strand, within HGI48_RS02375 at 514.338 kb on - strand, within HGI48_RS02375 at 514.338 kb on - strand, within HGI48_RS02375 at 514.338 kb on - strand, within HGI48_RS02375 at 514.343 kb on + strand, within HGI48_RS02375 at 514.343 kb on + strand, within HGI48_RS02375 at 514.344 kb on - strand, within HGI48_RS02375 at 514.344 kb on - strand, within HGI48_RS02375 at 514.344 kb on - strand, within HGI48_RS02375 at 514.344 kb on - strand, within HGI48_RS02375 at 514.495 kb on + strand, within HGI48_RS02375 at 514.496 kb on - strand, within HGI48_RS02375 at 514.517 kb on + strand, within HGI48_RS02375 at 514.517 kb on + strand, within HGI48_RS02375 at 514.518 kb on - strand, within HGI48_RS02375 at 514.518 kb on - strand, within HGI48_RS02375 at 514.579 kb on + strand, within HGI48_RS02375 at 514.580 kb on - strand, within HGI48_RS02375 at 514.610 kb on - strand, within HGI48_RS02375 at 514.623 kb on + strand, within HGI48_RS02375 at 514.624 kb on - strand, within HGI48_RS02375 at 514.624 kb on - strand, within HGI48_RS02375 at 514.660 kb on + strand, within HGI48_RS02375 at 514.660 kb on + strand, within HGI48_RS02375 at 514.660 kb on + strand, within HGI48_RS02375 at 514.660 kb on + strand, within HGI48_RS02375 at 514.660 kb on + strand, within HGI48_RS02375 at 514.660 kb on + strand, within HGI48_RS02375 at 514.660 kb on + strand, within HGI48_RS02375 at 514.660 kb on + strand, within HGI48_RS02375 at 514.660 kb on + strand, within HGI48_RS02375 at 514.660 kb on + strand, within HGI48_RS02375 at 514.660 kb on + strand, within HGI48_RS02375 at 514.661 kb on - strand at 514.661 kb on - strand, within HGI48_RS02375 at 514.700 kb on + strand, within HGI48_RS02375 at 514.796 kb on + strand, within HGI48_RS02375 at 514.796 kb on + strand, within HGI48_RS02375 at 514.796 kb on + strand, within HGI48_RS02375 at 514.796 kb on + strand, within HGI48_RS02375 at 514.796 kb on + strand, within HGI48_RS02375 at 514.797 kb on - strand at 514.797 kb on - strand, within HGI48_RS02375 at 514.797 kb on - strand, within HGI48_RS02375 at 514.797 kb on - strand, within HGI48_RS02375 at 514.797 kb on - strand, within HGI48_RS02375 at 514.797 kb on - strand, within HGI48_RS02375 at 514.797 kb on - strand, within HGI48_RS02375 at 514.798 kb on + strand, within HGI48_RS02375 at 514.798 kb on + strand, within HGI48_RS02375 at 514.798 kb on + strand at 514.799 kb on - strand, within HGI48_RS02375 at 514.799 kb on - strand at 514.799 kb on - strand, within HGI48_RS02375 at 514.799 kb on - strand, within HGI48_RS02375 at 514.799 kb on - strand, within HGI48_RS02375 at 514.799 kb on - strand, within HGI48_RS02375 at 514.799 kb on - strand, within HGI48_RS02375 at 514.850 kb on + strand, within HGI48_RS02375 at 514.851 kb on - strand at 514.851 kb on - strand, within HGI48_RS02375 at 514.901 kb on + strand, within HGI48_RS02375 at 514.901 kb on + strand, within HGI48_RS02375 at 514.901 kb on + strand, within HGI48_RS02375 at 514.901 kb on + strand, within HGI48_RS02375 at 514.902 kb on - strand, within HGI48_RS02375 at 514.902 kb on - strand at 514.902 kb on - strand, within HGI48_RS02375 at 514.902 kb on - strand, within HGI48_RS02375 at 514.903 kb on + strand, within HGI48_RS02375 at 514.903 kb on + strand at 514.903 kb on + strand, within HGI48_RS02375 at 514.903 kb on + strand, within HGI48_RS02375 at 514.903 kb on + strand, within HGI48_RS02375 at 514.904 kb on - strand, within HGI48_RS02375 at 514.904 kb on - strand, within HGI48_RS02375 at 514.904 kb on - strand, within HGI48_RS02375 at 514.904 kb on - strand, within HGI48_RS02375 at 515.063 kb on - strand, within HGI48_RS02375 at 515.188 kb on - strand, within HGI48_RS02375
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 512,626 - -0.4 512,657 + +0.5 512,657 + -0.5 512,657 + +0.2 512,658 - +0.6 512,658 - -0.8 512,658 - -1.0 512,664 + +0.2 512,667 - -0.9 512,707 - -0.9 512,711 + -0.4 512,730 + +0.7 512,731 - -1.8 512,731 - +0.6 512,732 + -2.3 512,770 + -0.8 512,770 + -1.8 512,770 + -0.8 512,770 + -0.0 512,770 + -0.4 512,771 - -1.1 512,771 - -1.6 512,777 + -0.3 512,777 + +0.7 512,777 + +1.5 512,778 - -0.8 512,778 - -1.3 512,778 - -0.4 512,778 - +1.2 512,788 + -0.8 512,788 + +1.6 512,788 + -0.7 512,788 + +1.0 512,788 + -1.8 512,788 + -2.1 512,789 - -0.8 512,789 - -1.4 512,789 - -1.1 512,795 + -1.1 512,795 + +0.0 512,813 + -1.5 512,813 + +1.6 512,813 + -1.1 512,813 + +0.2 512,813 + -0.8 512,815 + -0.3 512,815 + -0.9 512,815 + -0.6 512,820 + +0.9 513,235 - +0.1 513,296 + +0.2 513,296 + -0.0 513,296 + +1.2 513,297 - -0.4 513,297 - -0.8 513,297 - -0.8 513,297 - +1.2 513,297 - -0.6 513,297 - -1.5 513,297 - -1.7 513,301 + -2.4 513,302 - -0.4 513,302 - -0.3 513,381 + +0.4 513,381 + +0.5 513,382 - +0.6 513,382 - -1.1 513,382 - +0.1 513,428 - -1.4 513,531 + +1.2 513,531 + -1.0 513,531 + -1.6 513,544 + -0.1 513,561 - -0.8 513,621 + +2.2 513,830 + HGI48_RS02370 0.41 -1.1 513,830 + HGI48_RS02370 0.41 -0.8 513,831 - HGI48_RS02370 0.42 +1.9 513,831 - HGI48_RS02370 0.42 -1.3 513,876 + HGI48_RS02370 0.49 -1.8 513,877 - HGI48_RS02370 0.49 +1.2 513,877 - HGI48_RS02370 0.49 -1.1 514,041 - HGI48_RS02370 0.76 -1.0 514,041 - HGI48_RS02370 0.76 -1.8 514,193 - +1.8 514,289 - +0.2 514,289 - -0.4 514,312 + -0.6 514,312 + +1.2 514,312 + -2.1 514,312 + -1.4 514,312 + +0.2 514,312 + -0.8 514,313 - +0.7 514,313 - -0.8 514,313 - -1.6 514,317 + +1.8 514,318 - -0.6 514,337 + HGI48_RS02375 0.11 +1.9 514,337 + HGI48_RS02375 0.11 -1.0 514,337 + HGI48_RS02375 0.11 -0.8 514,337 + HGI48_RS02375 0.11 +0.8 514,337 + HGI48_RS02375 0.11 +0.7 514,338 - HGI48_RS02375 0.11 -0.5 514,338 - HGI48_RS02375 0.11 +0.1 514,338 - HGI48_RS02375 0.11 -0.0 514,338 - HGI48_RS02375 0.11 -1.8 514,338 - HGI48_RS02375 0.11 +0.9 514,343 + HGI48_RS02375 0.11 +0.2 514,343 + HGI48_RS02375 0.11 +1.0 514,344 - HGI48_RS02375 0.11 +0.2 514,344 - HGI48_RS02375 0.11 -0.4 514,344 - HGI48_RS02375 0.11 -2.1 514,344 - HGI48_RS02375 0.11 -1.9 514,495 + HGI48_RS02375 0.23 -0.5 514,496 - HGI48_RS02375 0.23 -0.1 514,517 + HGI48_RS02375 0.25 -1.1 514,517 + HGI48_RS02375 0.25 +2.2 514,518 - HGI48_RS02375 0.25 -0.9 514,518 - HGI48_RS02375 0.25 -0.4 514,579 + HGI48_RS02375 0.30 +0.2 514,580 - HGI48_RS02375 0.30 +1.2 514,610 - HGI48_RS02375 0.32 +0.3 514,623 + HGI48_RS02375 0.33 -0.5 514,624 - HGI48_RS02375 0.33 -0.0 514,624 - HGI48_RS02375 0.33 -1.4 514,660 + HGI48_RS02375 0.36 -1.1 514,660 + HGI48_RS02375 0.36 -1.1 514,660 + HGI48_RS02375 0.36 +0.5 514,660 + HGI48_RS02375 0.36 -0.6 514,660 + HGI48_RS02375 0.36 -0.8 514,660 + HGI48_RS02375 0.36 +0.7 514,660 + HGI48_RS02375 0.36 -0.4 514,660 + HGI48_RS02375 0.36 -0.9 514,660 + HGI48_RS02375 0.36 -0.9 514,660 + HGI48_RS02375 0.36 -1.4 514,660 + HGI48_RS02375 0.36 -1.8 514,661 - -0.4 514,661 - HGI48_RS02375 0.36 -2.0 514,700 + HGI48_RS02375 0.40 +1.2 514,796 + HGI48_RS02375 0.47 -1.4 514,796 + HGI48_RS02375 0.47 +0.8 514,796 + HGI48_RS02375 0.47 -2.0 514,796 + HGI48_RS02375 0.47 +0.2 514,796 + HGI48_RS02375 0.47 -2.2 514,797 - +3.8 514,797 - HGI48_RS02375 0.47 +0.9 514,797 - HGI48_RS02375 0.47 +2.0 514,797 - HGI48_RS02375 0.47 -1.0 514,797 - HGI48_RS02375 0.47 -2.1 514,797 - HGI48_RS02375 0.47 -2.0 514,797 - HGI48_RS02375 0.47 -1.4 514,798 + HGI48_RS02375 0.47 -0.4 514,798 + HGI48_RS02375 0.47 -2.4 514,798 + +0.6 514,799 - HGI48_RS02375 0.47 -0.3 514,799 - +1.2 514,799 - HGI48_RS02375 0.47 -2.4 514,799 - HGI48_RS02375 0.47 -1.2 514,799 - HGI48_RS02375 0.47 -0.4 514,799 - HGI48_RS02375 0.47 -1.8 514,799 - HGI48_RS02375 0.47 -0.4 514,850 + HGI48_RS02375 0.51 -1.1 514,851 - +1.2 514,851 - HGI48_RS02375 0.52 -1.4 514,901 + HGI48_RS02375 0.56 +0.8 514,901 + HGI48_RS02375 0.56 -0.7 514,901 + HGI48_RS02375 0.56 +1.4 514,901 + HGI48_RS02375 0.56 -0.8 514,902 - HGI48_RS02375 0.56 -0.6 514,902 - -0.4 514,902 - HGI48_RS02375 0.56 -1.1 514,902 - HGI48_RS02375 0.56 +0.0 514,903 + HGI48_RS02375 0.56 -1.1 514,903 + -0.4 514,903 + HGI48_RS02375 0.56 -2.1 514,903 + HGI48_RS02375 0.56 -0.5 514,903 + HGI48_RS02375 0.56 -1.1 514,904 - HGI48_RS02375 0.56 -0.3 514,904 - HGI48_RS02375 0.56 -2.8 514,904 - HGI48_RS02375 0.56 -2.1 514,904 - HGI48_RS02375 0.56 -1.4 515,063 - HGI48_RS02375 0.68 -1.1 515,188 - HGI48_RS02375 0.78 -0.4
Or see this region's nucleotide sequence