Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01410 and HGI48_RS01415 are separated by 190 nucleotides HGI48_RS01415 and HGI48_RS01420 are separated by 66 nucleotides HGI48_RS01420 and HGI48_RS01425 are separated by 178 nucleotides HGI48_RS01425 and HGI48_RS01430 overlap by 4 nucleotides
HGI48_RS01410: HGI48_RS01410 - ribosome-associated protein, at 315,778 to 316,326
_RS01410
HGI48_RS01415: HGI48_RS01415 - metalloprotease PmbA, at 316,517 to 317,857
_RS01415
HGI48_RS01420: HGI48_RS01420 - nucleoside diphosphate kinase regulator, at 317,924 to 318,334
_RS01420
HGI48_RS01425: HGI48_RS01425 - PTS phosphocarrier protein NPr, at 318,513 to 318,785
_RS01425
HGI48_RS01430: HGI48_RS01430 - RNase adapter RapZ, at 318,782 to 319,636
_RS01430
Position (kb) 316
317
318 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 315.535 kb on - strand at 315.538 kb on - strand at 315.568 kb on + strand at 315.568 kb on + strand at 315.568 kb on + strand at 315.569 kb on - strand at 315.569 kb on - strand at 315.569 kb on - strand at 315.569 kb on - strand at 315.569 kb on - strand at 315.569 kb on - strand at 315.597 kb on + strand at 315.598 kb on - strand at 315.598 kb on - strand at 315.731 kb on + strand at 315.731 kb on + strand at 315.731 kb on + strand at 315.731 kb on + strand at 315.731 kb on + strand at 315.731 kb on + strand at 315.731 kb on + strand at 315.731 kb on + strand at 315.731 kb on + strand at 315.732 kb on - strand at 315.732 kb on - strand at 315.732 kb on - strand at 315.732 kb on - strand at 315.732 kb on - strand at 315.733 kb on + strand at 315.733 kb on + strand at 315.733 kb on + strand at 315.734 kb on - strand at 315.734 kb on - strand at 315.761 kb on + strand at 315.776 kb on + strand at 315.776 kb on + strand at 315.777 kb on - strand at 315.777 kb on - strand at 315.777 kb on - strand at 315.777 kb on - strand at 315.777 kb on - strand at 315.777 kb on - strand at 315.779 kb on + strand at 315.806 kb on + strand at 316.255 kb on + strand, within HGI48_RS01410 at 316.255 kb on + strand, within HGI48_RS01410 at 316.255 kb on + strand, within HGI48_RS01410 at 316.255 kb on + strand, within HGI48_RS01410 at 316.256 kb on - strand, within HGI48_RS01410 at 316.340 kb on + strand at 316.340 kb on + strand at 316.400 kb on - strand at 316.416 kb on + strand at 316.416 kb on + strand at 316.416 kb on + strand at 316.416 kb on + strand at 316.416 kb on + strand at 316.417 kb on - strand at 316.417 kb on - strand at 316.417 kb on - strand at 316.427 kb on - strand at 316.438 kb on - strand at 316.438 kb on - strand at 316.482 kb on + strand at 316.482 kb on + strand at 316.482 kb on + strand at 316.483 kb on - strand at 316.483 kb on - strand at 316.498 kb on + strand at 316.524 kb on + strand at 316.524 kb on + strand at 316.525 kb on - strand at 316.715 kb on + strand, within HGI48_RS01415 at 316.716 kb on - strand, within HGI48_RS01415 at 316.718 kb on + strand, within HGI48_RS01415 at 316.719 kb on - strand, within HGI48_RS01415 at 316.809 kb on - strand, within HGI48_RS01415 at 316.993 kb on + strand, within HGI48_RS01415 at 316.994 kb on - strand, within HGI48_RS01415 at 316.994 kb on - strand, within HGI48_RS01415 at 317.003 kb on + strand, within HGI48_RS01415 at 317.003 kb on + strand, within HGI48_RS01415 at 317.035 kb on + strand, within HGI48_RS01415 at 317.035 kb on + strand, within HGI48_RS01415 at 317.035 kb on + strand, within HGI48_RS01415 at 317.035 kb on + strand, within HGI48_RS01415 at 317.048 kb on + strand, within HGI48_RS01415 at 317.048 kb on + strand, within HGI48_RS01415 at 317.048 kb on + strand at 317.048 kb on + strand, within HGI48_RS01415 at 317.118 kb on - strand at 317.118 kb on - strand, within HGI48_RS01415 at 317.123 kb on + strand, within HGI48_RS01415 at 317.123 kb on + strand, within HGI48_RS01415 at 317.124 kb on - strand, within HGI48_RS01415 at 317.192 kb on - strand, within HGI48_RS01415 at 317.306 kb on - strand, within HGI48_RS01415 at 317.306 kb on - strand, within HGI48_RS01415 at 317.317 kb on + strand, within HGI48_RS01415 at 317.317 kb on + strand, within HGI48_RS01415 at 317.318 kb on - strand, within HGI48_RS01415 at 317.318 kb on - strand, within HGI48_RS01415 at 317.318 kb on - strand, within HGI48_RS01415 at 317.472 kb on + strand, within HGI48_RS01415 at 317.473 kb on - strand, within HGI48_RS01415 at 317.498 kb on + strand, within HGI48_RS01415 at 317.498 kb on + strand, within HGI48_RS01415 at 317.498 kb on + strand, within HGI48_RS01415 at 317.499 kb on - strand, within HGI48_RS01415 at 317.499 kb on - strand, within HGI48_RS01415 at 317.499 kb on - strand, within HGI48_RS01415 at 317.499 kb on - strand, within HGI48_RS01415 at 317.551 kb on + strand, within HGI48_RS01415 at 317.551 kb on + strand, within HGI48_RS01415 at 317.552 kb on - strand, within HGI48_RS01415 at 317.666 kb on + strand, within HGI48_RS01415 at 317.755 kb on + strand at 317.756 kb on - strand at 317.756 kb on - strand at 317.756 kb on - strand at 317.756 kb on - strand at 317.756 kb on - strand at 317.853 kb on + strand at 317.853 kb on + strand at 317.853 kb on + strand at 317.855 kb on + strand at 317.855 kb on + strand at 317.855 kb on + strand at 317.855 kb on + strand at 317.855 kb on + strand at 317.855 kb on + strand at 317.856 kb on - strand at 317.856 kb on - strand at 317.856 kb on - strand at 317.856 kb on - strand at 317.856 kb on - strand at 317.856 kb on - strand at 317.862 kb on + strand at 317.862 kb on + strand at 317.863 kb on - strand at 317.863 kb on - strand at 317.863 kb on - strand at 317.934 kb on + strand at 317.934 kb on + strand at 317.935 kb on - strand at 317.935 kb on - strand at 317.935 kb on - strand at 318.016 kb on + strand, within HGI48_RS01420 at 318.016 kb on + strand, within HGI48_RS01420 at 318.016 kb on + strand at 318.017 kb on - strand, within HGI48_RS01420 at 318.017 kb on - strand, within HGI48_RS01420 at 318.017 kb on - strand, within HGI48_RS01420 at 318.017 kb on - strand, within HGI48_RS01420 at 318.017 kb on - strand, within HGI48_RS01420 at 318.121 kb on + strand, within HGI48_RS01420 at 318.121 kb on + strand, within HGI48_RS01420 at 318.122 kb on - strand, within HGI48_RS01420 at 318.122 kb on - strand, within HGI48_RS01420 at 318.328 kb on + strand at 318.334 kb on + strand at 318.334 kb on + strand at 318.335 kb on - strand at 318.335 kb on - strand at 318.335 kb on - strand at 318.356 kb on + strand at 318.357 kb on - strand at 318.364 kb on - strand at 318.393 kb on + strand at 318.393 kb on + strand at 318.393 kb on + strand at 318.393 kb on + strand at 318.393 kb on + strand at 318.393 kb on + strand at 318.393 kb on + strand at 318.393 kb on + strand at 318.393 kb on + strand at 318.393 kb on + strand at 318.393 kb on + strand at 318.394 kb on - strand at 318.394 kb on - strand at 318.394 kb on - strand at 318.394 kb on - strand at 318.394 kb on - strand at 318.394 kb on - strand at 318.394 kb on - strand at 318.410 kb on - strand at 318.429 kb on + strand at 318.430 kb on - strand at 318.430 kb on - strand at 318.436 kb on + strand at 318.451 kb on + strand at 318.486 kb on + strand at 318.487 kb on - strand at 318.504 kb on + strand at 318.504 kb on + strand at 318.505 kb on - strand at 318.505 kb on - strand at 318.505 kb on - strand at 318.514 kb on + strand at 318.515 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 315,535 - -0.7 315,538 - -0.9 315,568 + -0.1 315,568 + +0.8 315,568 + -1.3 315,569 - -0.9 315,569 - -0.2 315,569 - -0.3 315,569 - -1.7 315,569 - -2.2 315,569 - -2.8 315,597 + +1.2 315,598 - -1.1 315,598 - -0.8 315,731 + +0.5 315,731 + -0.8 315,731 + -0.3 315,731 + -0.8 315,731 + -1.3 315,731 + +0.2 315,731 + -0.3 315,731 + +0.0 315,731 + +0.8 315,732 - -1.1 315,732 - -0.6 315,732 - +2.7 315,732 - +1.2 315,732 - -0.2 315,733 + -1.3 315,733 + +0.5 315,733 + -0.5 315,734 - -1.3 315,734 - -0.4 315,761 + -2.9 315,776 + -1.8 315,776 + -0.3 315,777 - -1.1 315,777 - -1.1 315,777 - -0.2 315,777 - +1.2 315,777 - -0.1 315,777 - -1.3 315,779 + -0.4 315,806 + -1.6 316,255 + HGI48_RS01410 0.87 -1.1 316,255 + HGI48_RS01410 0.87 -0.9 316,255 + HGI48_RS01410 0.87 +0.6 316,255 + HGI48_RS01410 0.87 -1.8 316,256 - HGI48_RS01410 0.87 -0.2 316,340 + +0.4 316,340 + -0.3 316,400 - +0.3 316,416 + -1.3 316,416 + -1.8 316,416 + -2.7 316,416 + -0.7 316,416 + +0.2 316,417 - +1.0 316,417 - -0.8 316,417 - +0.1 316,427 - -1.1 316,438 - -1.0 316,438 - -1.1 316,482 + +1.7 316,482 + -1.3 316,482 + +0.9 316,483 - -1.7 316,483 - +0.4 316,498 + -0.9 316,524 + +0.1 316,524 + -0.8 316,525 - -0.1 316,715 + HGI48_RS01415 0.15 +0.0 316,716 - HGI48_RS01415 0.15 -0.8 316,718 + HGI48_RS01415 0.15 -2.2 316,719 - HGI48_RS01415 0.15 +0.9 316,809 - HGI48_RS01415 0.22 -0.2 316,993 + HGI48_RS01415 0.35 -1.3 316,994 - HGI48_RS01415 0.36 -0.8 316,994 - HGI48_RS01415 0.36 +0.2 317,003 + HGI48_RS01415 0.36 +0.1 317,003 + HGI48_RS01415 0.36 +0.0 317,035 + HGI48_RS01415 0.39 +0.9 317,035 + HGI48_RS01415 0.39 -0.1 317,035 + HGI48_RS01415 0.39 -2.2 317,035 + HGI48_RS01415 0.39 -1.8 317,048 + HGI48_RS01415 0.40 -1.6 317,048 + HGI48_RS01415 0.40 -0.3 317,048 + +0.2 317,048 + HGI48_RS01415 0.40 +0.2 317,118 - -0.3 317,118 - HGI48_RS01415 0.45 -0.8 317,123 + HGI48_RS01415 0.45 -1.3 317,123 + HGI48_RS01415 0.45 -0.6 317,124 - HGI48_RS01415 0.45 +1.2 317,192 - HGI48_RS01415 0.50 +0.1 317,306 - HGI48_RS01415 0.59 -0.3 317,306 - HGI48_RS01415 0.59 -1.6 317,317 + HGI48_RS01415 0.60 -1.5 317,317 + HGI48_RS01415 0.60 -1.8 317,318 - HGI48_RS01415 0.60 -1.1 317,318 - HGI48_RS01415 0.60 -2.3 317,318 - HGI48_RS01415 0.60 -0.8 317,472 + HGI48_RS01415 0.71 -0.5 317,473 - HGI48_RS01415 0.71 -0.8 317,498 + HGI48_RS01415 0.73 +0.9 317,498 + HGI48_RS01415 0.73 +0.0 317,498 + HGI48_RS01415 0.73 +1.2 317,499 - HGI48_RS01415 0.73 -0.9 317,499 - HGI48_RS01415 0.73 -1.5 317,499 - HGI48_RS01415 0.73 +1.2 317,499 - HGI48_RS01415 0.73 -0.2 317,551 + HGI48_RS01415 0.77 +1.0 317,551 + HGI48_RS01415 0.77 +1.2 317,552 - HGI48_RS01415 0.77 +2.1 317,666 + HGI48_RS01415 0.86 -2.5 317,755 + -0.6 317,756 - -1.3 317,756 - -1.6 317,756 - -1.3 317,756 - -0.3 317,756 - -1.6 317,853 + -1.8 317,853 + -0.7 317,853 + -0.3 317,855 + +0.7 317,855 + -1.8 317,855 + -0.1 317,855 + +1.2 317,855 + +0.5 317,855 + -1.6 317,856 - -0.6 317,856 - -0.5 317,856 - -1.6 317,856 - +0.8 317,856 - -0.3 317,856 - +0.9 317,862 + -0.3 317,862 + -0.5 317,863 - +0.7 317,863 - -0.1 317,863 - +0.5 317,934 + -0.6 317,934 + +0.9 317,935 - +0.2 317,935 - -1.1 317,935 - -0.5 318,016 + HGI48_RS01420 0.22 +0.6 318,016 + HGI48_RS01420 0.22 +0.1 318,016 + +1.8 318,017 - HGI48_RS01420 0.23 -0.8 318,017 - HGI48_RS01420 0.23 -1.0 318,017 - HGI48_RS01420 0.23 -0.6 318,017 - HGI48_RS01420 0.23 -1.1 318,017 - HGI48_RS01420 0.23 +0.7 318,121 + HGI48_RS01420 0.48 +0.3 318,121 + HGI48_RS01420 0.48 -0.2 318,122 - HGI48_RS01420 0.48 -1.1 318,122 - HGI48_RS01420 0.48 -3.7 318,328 + -1.9 318,334 + +1.8 318,334 + -0.3 318,335 - -2.3 318,335 - +1.2 318,335 - -0.2 318,356 + -1.3 318,357 - +1.2 318,364 - +0.9 318,393 + -0.5 318,393 + -1.4 318,393 + +1.2 318,393 + +0.2 318,393 + -0.9 318,393 + +0.9 318,393 + +0.3 318,393 + -1.6 318,393 + -0.9 318,393 + -1.2 318,393 + -0.8 318,394 - -1.1 318,394 - -0.8 318,394 - +0.5 318,394 - -1.2 318,394 - +0.2 318,394 - +0.5 318,394 - -1.9 318,410 - -0.8 318,429 + -0.8 318,430 - -0.3 318,430 - -2.5 318,436 + +0.1 318,451 + -1.8 318,486 + +1.4 318,487 - +0.1 318,504 + -1.9 318,504 + +1.2 318,505 - -1.1 318,505 - -1.0 318,505 - +1.4 318,514 + -0.6 318,515 - +0.9
Or see this region's nucleotide sequence