Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS18165 and HGI48_RS18170 are separated by 259 nucleotides HGI48_RS18170 and HGI48_RS18175 are separated by 63 nucleotides HGI48_RS18175 and HGI48_RS18180 are separated by 64 nucleotides
HGI48_RS18165: HGI48_RS18165 - DNA polymerase II, at 4,072,517 to 4,074,892
_RS18165
HGI48_RS18170: HGI48_RS18170 - RNA polymerase-associated protein RapA, at 4,075,152 to 4,078,055
_RS18170
HGI48_RS18175: HGI48_RS18175 - bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA, at 4,078,119 to 4,078,772
_RS18175
HGI48_RS18180: HGI48_RS18180 - co-chaperone DjlA, at 4,078,837 to 4,079,859
_RS18180
Position (kb)
4075
4076
4077
4078
4079 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4074.161 kb on + strand, within HGI48_RS18165 at 4074.248 kb on + strand, within HGI48_RS18165 at 4074.249 kb on - strand, within HGI48_RS18165 at 4074.498 kb on - strand, within HGI48_RS18165 at 4074.524 kb on + strand, within HGI48_RS18165 at 4074.546 kb on - strand, within HGI48_RS18165 at 4074.549 kb on + strand, within HGI48_RS18165 at 4074.549 kb on + strand, within HGI48_RS18165 at 4074.572 kb on + strand, within HGI48_RS18165 at 4074.674 kb on + strand at 4074.674 kb on + strand at 4074.675 kb on - strand at 4074.675 kb on - strand at 4074.675 kb on - strand at 4074.728 kb on + strand at 4074.728 kb on + strand at 4074.729 kb on - strand at 4074.729 kb on - strand at 4074.729 kb on - strand at 4074.729 kb on - strand at 4074.729 kb on - strand at 4074.729 kb on - strand at 4074.729 kb on - strand at 4074.729 kb on - strand at 4074.729 kb on - strand at 4074.729 kb on - strand at 4074.729 kb on - strand at 4074.741 kb on + strand at 4074.742 kb on - strand at 4074.742 kb on - strand at 4074.742 kb on - strand at 4074.785 kb on + strand at 4074.795 kb on - strand at 4074.795 kb on - strand at 4074.799 kb on - strand at 4074.799 kb on - strand at 4074.829 kb on + strand at 4074.830 kb on - strand at 4074.859 kb on + strand at 4074.885 kb on + strand at 4074.886 kb on - strand at 4074.890 kb on + strand at 4074.890 kb on + strand at 4074.891 kb on - strand at 4075.002 kb on + strand at 4075.002 kb on + strand at 4075.002 kb on + strand at 4075.002 kb on + strand at 4075.003 kb on - strand at 4075.003 kb on - strand at 4075.003 kb on - strand at 4075.078 kb on - strand at 4075.152 kb on - strand at 4075.160 kb on + strand at 4075.161 kb on - strand at 4075.201 kb on + strand at 4075.202 kb on - strand at 4075.278 kb on + strand at 4075.278 kb on + strand at 4075.335 kb on - strand at 4075.398 kb on + strand at 4075.398 kb on + strand at 4075.398 kb on + strand at 4075.667 kb on - strand, within HGI48_RS18170 at 4075.667 kb on - strand, within HGI48_RS18170 at 4075.719 kb on - strand, within HGI48_RS18170 at 4076.034 kb on + strand, within HGI48_RS18170 at 4076.035 kb on - strand, within HGI48_RS18170 at 4076.035 kb on - strand, within HGI48_RS18170 at 4076.456 kb on - strand at 4076.478 kb on + strand, within HGI48_RS18170 at 4076.479 kb on - strand, within HGI48_RS18170 at 4076.520 kb on - strand, within HGI48_RS18170 at 4076.569 kb on - strand, within HGI48_RS18170 at 4076.569 kb on - strand, within HGI48_RS18170 at 4076.569 kb on - strand, within HGI48_RS18170 at 4076.569 kb on - strand at 4076.569 kb on - strand, within HGI48_RS18170 at 4076.793 kb on + strand, within HGI48_RS18170 at 4076.842 kb on + strand, within HGI48_RS18170 at 4076.843 kb on - strand, within HGI48_RS18170 at 4077.076 kb on - strand, within HGI48_RS18170 at 4077.146 kb on + strand, within HGI48_RS18170 at 4077.147 kb on - strand, within HGI48_RS18170 at 4077.147 kb on - strand at 4077.278 kb on + strand, within HGI48_RS18170 at 4077.279 kb on - strand, within HGI48_RS18170 at 4077.383 kb on + strand, within HGI48_RS18170 at 4077.383 kb on + strand, within HGI48_RS18170 at 4077.384 kb on - strand, within HGI48_RS18170 at 4077.384 kb on - strand, within HGI48_RS18170 at 4077.384 kb on - strand, within HGI48_RS18170 at 4077.384 kb on - strand, within HGI48_RS18170 at 4077.604 kb on + strand, within HGI48_RS18170 at 4077.606 kb on + strand, within HGI48_RS18170 at 4077.606 kb on + strand, within HGI48_RS18170 at 4077.606 kb on + strand, within HGI48_RS18170 at 4077.606 kb on + strand, within HGI48_RS18170 at 4077.606 kb on + strand, within HGI48_RS18170 at 4077.606 kb on + strand, within HGI48_RS18170 at 4077.606 kb on + strand, within HGI48_RS18170 at 4077.606 kb on + strand, within HGI48_RS18170 at 4077.607 kb on - strand, within HGI48_RS18170 at 4077.607 kb on - strand, within HGI48_RS18170 at 4077.607 kb on - strand, within HGI48_RS18170 at 4077.607 kb on - strand, within HGI48_RS18170 at 4077.607 kb on - strand, within HGI48_RS18170 at 4077.607 kb on - strand, within HGI48_RS18170 at 4077.607 kb on - strand, within HGI48_RS18170 at 4078.053 kb on + strand at 4078.054 kb on - strand at 4078.054 kb on - strand at 4078.071 kb on + strand at 4078.072 kb on - strand at 4078.075 kb on - strand at 4078.075 kb on - strand at 4078.075 kb on - strand at 4078.128 kb on + strand at 4078.128 kb on + strand at 4078.129 kb on - strand at 4078.129 kb on - strand at 4078.398 kb on + strand, within HGI48_RS18175 at 4078.444 kb on + strand, within HGI48_RS18175 at 4078.529 kb on + strand, within HGI48_RS18175 at 4078.529 kb on + strand, within HGI48_RS18175 at 4078.530 kb on - strand at 4078.530 kb on - strand, within HGI48_RS18175 at 4078.604 kb on - strand, within HGI48_RS18175 at 4078.653 kb on + strand, within HGI48_RS18175 at 4078.896 kb on + strand at 4078.896 kb on + strand at 4078.980 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 4,074,161 + HGI48_RS18165 0.69 -0.6 4,074,248 + HGI48_RS18165 0.73 -0.3 4,074,249 - HGI48_RS18165 0.73 +0.7 4,074,498 - HGI48_RS18165 0.83 -1.6 4,074,524 + HGI48_RS18165 0.84 -1.0 4,074,546 - HGI48_RS18165 0.85 -0.8 4,074,549 + HGI48_RS18165 0.86 +0.6 4,074,549 + HGI48_RS18165 0.86 -0.3 4,074,572 + HGI48_RS18165 0.86 -2.6 4,074,674 + +1.5 4,074,674 + -2.4 4,074,675 - -1.3 4,074,675 - -1.3 4,074,675 - +0.0 4,074,728 + -0.4 4,074,728 + +0.4 4,074,729 - +3.8 4,074,729 - -0.6 4,074,729 - -1.6 4,074,729 - +0.4 4,074,729 - -0.6 4,074,729 - +0.0 4,074,729 - +1.2 4,074,729 - -0.7 4,074,729 - +0.0 4,074,729 - -0.4 4,074,729 - -1.0 4,074,741 + -1.0 4,074,742 - +0.0 4,074,742 - +1.5 4,074,742 - -1.0 4,074,785 + -0.6 4,074,795 - +1.0 4,074,795 - +0.2 4,074,799 - +0.5 4,074,799 - -1.0 4,074,829 + +1.0 4,074,830 - +0.3 4,074,859 + +1.4 4,074,885 + +1.0 4,074,886 - +1.0 4,074,890 + +0.4 4,074,890 + -0.6 4,074,891 - -1.1 4,075,002 + -0.2 4,075,002 + +0.0 4,075,002 + -0.6 4,075,002 + -1.3 4,075,003 - -3.5 4,075,003 - -0.2 4,075,003 - +0.0 4,075,078 - +1.6 4,075,152 - +1.0 4,075,160 + +0.0 4,075,161 - -0.7 4,075,201 + -1.0 4,075,202 - +0.0 4,075,278 + -2.0 4,075,278 + +0.0 4,075,335 - +0.4 4,075,398 + +1.6 4,075,398 + +0.1 4,075,398 + +0.2 4,075,667 - HGI48_RS18170 0.18 -2.8 4,075,667 - HGI48_RS18170 0.18 -1.3 4,075,719 - HGI48_RS18170 0.20 +1.9 4,076,034 + HGI48_RS18170 0.30 -1.0 4,076,035 - HGI48_RS18170 0.30 +0.1 4,076,035 - HGI48_RS18170 0.30 +2.5 4,076,456 - +2.3 4,076,478 + HGI48_RS18170 0.46 +0.7 4,076,479 - HGI48_RS18170 0.46 -0.3 4,076,520 - HGI48_RS18170 0.47 -1.0 4,076,569 - HGI48_RS18170 0.49 -1.0 4,076,569 - HGI48_RS18170 0.49 -0.1 4,076,569 - HGI48_RS18170 0.49 +0.6 4,076,569 - +1.6 4,076,569 - HGI48_RS18170 0.49 -2.1 4,076,793 + HGI48_RS18170 0.57 -0.2 4,076,842 + HGI48_RS18170 0.58 +0.0 4,076,843 - HGI48_RS18170 0.58 -2.3 4,077,076 - HGI48_RS18170 0.66 -0.1 4,077,146 + HGI48_RS18170 0.69 -0.1 4,077,147 - HGI48_RS18170 0.69 -2.4 4,077,147 - +1.0 4,077,278 + HGI48_RS18170 0.73 +0.8 4,077,279 - HGI48_RS18170 0.73 +0.3 4,077,383 + HGI48_RS18170 0.77 -1.0 4,077,383 + HGI48_RS18170 0.77 -1.0 4,077,384 - HGI48_RS18170 0.77 -1.3 4,077,384 - HGI48_RS18170 0.77 -0.3 4,077,384 - HGI48_RS18170 0.77 -1.0 4,077,384 - HGI48_RS18170 0.77 -1.6 4,077,604 + HGI48_RS18170 0.84 +0.4 4,077,606 + HGI48_RS18170 0.85 -1.3 4,077,606 + HGI48_RS18170 0.85 -1.3 4,077,606 + HGI48_RS18170 0.85 +1.0 4,077,606 + HGI48_RS18170 0.85 -0.3 4,077,606 + HGI48_RS18170 0.85 -0.4 4,077,606 + HGI48_RS18170 0.85 +1.4 4,077,606 + HGI48_RS18170 0.85 -0.5 4,077,606 + HGI48_RS18170 0.85 +0.4 4,077,607 - HGI48_RS18170 0.85 +1.5 4,077,607 - HGI48_RS18170 0.85 -1.7 4,077,607 - HGI48_RS18170 0.85 +0.4 4,077,607 - HGI48_RS18170 0.85 -0.6 4,077,607 - HGI48_RS18170 0.85 -0.1 4,077,607 - HGI48_RS18170 0.85 -1.0 4,077,607 - HGI48_RS18170 0.85 +0.6 4,078,053 + -3.2 4,078,054 - -1.3 4,078,054 - +0.7 4,078,071 + +1.0 4,078,072 - -0.6 4,078,075 - -0.3 4,078,075 - +0.1 4,078,075 - -1.3 4,078,128 + -1.6 4,078,128 + -1.3 4,078,129 - -1.3 4,078,129 - -0.6 4,078,398 + HGI48_RS18175 0.43 +0.6 4,078,444 + HGI48_RS18175 0.50 +0.4 4,078,529 + HGI48_RS18175 0.63 -1.3 4,078,529 + HGI48_RS18175 0.63 -1.0 4,078,530 - -0.6 4,078,530 - HGI48_RS18175 0.63 +0.0 4,078,604 - HGI48_RS18175 0.74 -0.8 4,078,653 + HGI48_RS18175 0.82 -1.3 4,078,896 + -1.0 4,078,896 + +1.0 4,078,980 - +2.3
Or see this region's nucleotide sequence