Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS14070 and HGI48_RS14075 are separated by 260 nucleotides HGI48_RS14075 and HGI48_RS14080 are separated by 47 nucleotides HGI48_RS14080 and HGI48_RS14085 are separated by 27 nucleotides
HGI48_RS14070: HGI48_RS14070 - 2,3-diaminopropionate biosynthesis protein SbnB, at 3,147,868 to 3,148,875
_RS14070
HGI48_RS14075: HGI48_RS14075 - NAD(P)H-dependent oxidoreductase, at 3,149,136 to 3,149,681
_RS14075
HGI48_RS14080: HGI48_RS14080 - uroporphyrinogen-III C-methyltransferase, at 3,149,729 to 3,150,559
_RS14080
HGI48_RS14085: HGI48_RS14085 - nitrate reductase, at 3,150,587 to 3,153,235
_RS14085
Position (kb)
3149
3150
3151 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3148.740 kb on + strand, within HGI48_RS14070 at 3148.743 kb on + strand, within HGI48_RS14070 at 3148.756 kb on + strand, within HGI48_RS14070 at 3148.841 kb on + strand at 3148.842 kb on - strand at 3148.845 kb on + strand at 3148.846 kb on - strand at 3148.895 kb on + strand at 3148.927 kb on + strand at 3148.977 kb on + strand at 3148.978 kb on - strand at 3148.978 kb on - strand at 3148.978 kb on - strand at 3148.978 kb on - strand at 3148.978 kb on - strand at 3148.999 kb on - strand at 3149.045 kb on + strand at 3149.046 kb on - strand at 3149.046 kb on - strand at 3149.050 kb on + strand at 3149.050 kb on + strand at 3149.051 kb on - strand at 3149.051 kb on - strand at 3149.051 kb on - strand at 3149.156 kb on - strand at 3149.160 kb on - strand at 3149.274 kb on + strand, within HGI48_RS14075 at 3149.274 kb on + strand, within HGI48_RS14075 at 3149.277 kb on - strand, within HGI48_RS14075 at 3149.368 kb on - strand, within HGI48_RS14075 at 3149.368 kb on - strand, within HGI48_RS14075 at 3149.368 kb on - strand, within HGI48_RS14075 at 3149.373 kb on + strand, within HGI48_RS14075 at 3149.374 kb on - strand, within HGI48_RS14075 at 3149.374 kb on - strand, within HGI48_RS14075 at 3149.748 kb on - strand at 3150.000 kb on + strand at 3150.000 kb on + strand, within HGI48_RS14080 at 3150.000 kb on + strand, within HGI48_RS14080 at 3150.001 kb on - strand, within HGI48_RS14080 at 3150.001 kb on - strand, within HGI48_RS14080 at 3150.001 kb on - strand, within HGI48_RS14080 at 3150.079 kb on + strand, within HGI48_RS14080 at 3150.194 kb on + strand, within HGI48_RS14080 at 3150.194 kb on + strand, within HGI48_RS14080 at 3150.195 kb on - strand, within HGI48_RS14080 at 3150.195 kb on - strand, within HGI48_RS14080 at 3150.198 kb on + strand, within HGI48_RS14080 at 3150.198 kb on + strand, within HGI48_RS14080 at 3150.198 kb on + strand at 3150.199 kb on - strand, within HGI48_RS14080 at 3150.199 kb on - strand, within HGI48_RS14080 at 3150.350 kb on - strand, within HGI48_RS14080 at 3150.350 kb on - strand at 3150.379 kb on + strand, within HGI48_RS14080 at 3150.379 kb on + strand, within HGI48_RS14080 at 3150.379 kb on + strand, within HGI48_RS14080 at 3150.380 kb on - strand, within HGI48_RS14080 at 3150.380 kb on - strand, within HGI48_RS14080 at 3150.382 kb on + strand at 3150.449 kb on - strand, within HGI48_RS14080 at 3150.550 kb on + strand at 3150.602 kb on + strand at 3150.603 kb on - strand at 3150.621 kb on + strand at 3150.621 kb on + strand at 3150.622 kb on - strand at 3150.622 kb on - strand at 3150.622 kb on - strand at 3150.906 kb on + strand, within HGI48_RS14085 at 3150.906 kb on + strand, within HGI48_RS14085 at 3150.906 kb on + strand, within HGI48_RS14085 at 3150.907 kb on - strand, within HGI48_RS14085 at 3150.907 kb on - strand, within HGI48_RS14085 at 3150.907 kb on - strand, within HGI48_RS14085 at 3150.907 kb on - strand, within HGI48_RS14085 at 3150.907 kb on - strand, within HGI48_RS14085 at 3150.907 kb on - strand, within HGI48_RS14085 at 3150.907 kb on - strand, within HGI48_RS14085 at 3150.907 kb on - strand, within HGI48_RS14085 at 3151.186 kb on + strand, within HGI48_RS14085 at 3151.186 kb on + strand, within HGI48_RS14085 at 3151.186 kb on + strand, within HGI48_RS14085 at 3151.187 kb on - strand, within HGI48_RS14085 at 3151.255 kb on + strand, within HGI48_RS14085 at 3151.416 kb on + strand, within HGI48_RS14085 at 3151.416 kb on + strand at 3151.417 kb on - strand, within HGI48_RS14085
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,148,740 + HGI48_RS14070 0.87 +1.0 3,148,743 + HGI48_RS14070 0.87 -1.7 3,148,756 + HGI48_RS14070 0.88 +0.2 3,148,841 + -0.2 3,148,842 - -0.9 3,148,845 + -0.3 3,148,846 - +1.2 3,148,895 + +0.8 3,148,927 + +0.3 3,148,977 + +0.9 3,148,978 - +1.3 3,148,978 - -0.4 3,148,978 - +2.2 3,148,978 - -0.7 3,148,978 - -0.8 3,148,999 - -1.0 3,149,045 + -2.4 3,149,046 - -2.0 3,149,046 - -0.7 3,149,050 + -0.0 3,149,050 + -0.4 3,149,051 - -0.4 3,149,051 - +1.0 3,149,051 - -1.0 3,149,156 - -0.2 3,149,160 - -0.4 3,149,274 + HGI48_RS14075 0.25 -0.0 3,149,274 + HGI48_RS14075 0.25 -2.7 3,149,277 - HGI48_RS14075 0.26 +0.4 3,149,368 - HGI48_RS14075 0.42 -1.4 3,149,368 - HGI48_RS14075 0.42 -0.2 3,149,368 - HGI48_RS14075 0.42 -1.4 3,149,373 + HGI48_RS14075 0.43 -0.2 3,149,374 - HGI48_RS14075 0.44 -2.4 3,149,374 - HGI48_RS14075 0.44 +0.9 3,149,748 - +0.0 3,150,000 + +2.0 3,150,000 + HGI48_RS14080 0.33 -1.1 3,150,000 + HGI48_RS14080 0.33 -1.4 3,150,001 - HGI48_RS14080 0.33 +0.9 3,150,001 - HGI48_RS14080 0.33 -0.7 3,150,001 - HGI48_RS14080 0.33 -2.0 3,150,079 + HGI48_RS14080 0.42 -1.4 3,150,194 + HGI48_RS14080 0.56 -0.8 3,150,194 + HGI48_RS14080 0.56 -0.4 3,150,195 - HGI48_RS14080 0.56 +1.0 3,150,195 - HGI48_RS14080 0.56 -1.2 3,150,198 + HGI48_RS14080 0.56 +0.8 3,150,198 + HGI48_RS14080 0.56 -1.5 3,150,198 + -1.0 3,150,199 - HGI48_RS14080 0.57 -0.0 3,150,199 - HGI48_RS14080 0.57 -2.4 3,150,350 - HGI48_RS14080 0.75 +0.6 3,150,350 - +1.0 3,150,379 + HGI48_RS14080 0.78 -1.4 3,150,379 + HGI48_RS14080 0.78 +0.2 3,150,379 + HGI48_RS14080 0.78 -0.8 3,150,380 - HGI48_RS14080 0.78 -2.0 3,150,380 - HGI48_RS14080 0.78 +1.3 3,150,382 + +1.0 3,150,449 - HGI48_RS14080 0.87 -1.4 3,150,550 + +0.6 3,150,602 + -2.0 3,150,603 - +0.0 3,150,621 + +1.5 3,150,621 + -1.0 3,150,622 - -0.1 3,150,622 - +0.0 3,150,622 - +0.3 3,150,906 + HGI48_RS14085 0.12 -1.4 3,150,906 + HGI48_RS14085 0.12 +0.2 3,150,906 + HGI48_RS14085 0.12 -0.7 3,150,907 - HGI48_RS14085 0.12 +1.2 3,150,907 - HGI48_RS14085 0.12 -0.5 3,150,907 - HGI48_RS14085 0.12 +0.4 3,150,907 - HGI48_RS14085 0.12 +0.9 3,150,907 - HGI48_RS14085 0.12 +0.4 3,150,907 - HGI48_RS14085 0.12 -1.2 3,150,907 - HGI48_RS14085 0.12 -0.6 3,150,907 - HGI48_RS14085 0.12 +0.8 3,151,186 + HGI48_RS14085 0.23 -1.1 3,151,186 + HGI48_RS14085 0.23 -0.5 3,151,186 + HGI48_RS14085 0.23 -2.7 3,151,187 - HGI48_RS14085 0.23 +1.5 3,151,255 + HGI48_RS14085 0.25 -2.2 3,151,416 + HGI48_RS14085 0.31 -0.3 3,151,416 + +0.1 3,151,417 - HGI48_RS14085 0.31 -2.5
Or see this region's nucleotide sequence