Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS05000 and HGI48_RS05005 are separated by 206 nucleotides HGI48_RS05005 and HGI48_RS05010 are separated by 81 nucleotides HGI48_RS05010 and HGI48_RS05015 are separated by 38 nucleotides HGI48_RS05015 and HGI48_RS05020 are separated by 210 nucleotides
HGI48_RS05000: HGI48_RS05000 - amino-acid N-acetyltransferase, at 1,049,794 to 1,051,137
_RS05000
HGI48_RS05005: HGI48_RS05005 - N-acetylmuramoyl-L-alanine amidase, at 1,051,344 to 1,052,582
_RS05005
HGI48_RS05010: HGI48_RS05010 - tRNA-Met, at 1,052,664 to 1,052,740
_RS05010
HGI48_RS05015: HGI48_RS05015 - tRNA-Met, at 1,052,779 to 1,052,855
_RS05015
HGI48_RS05020: HGI48_RS05020 - murein transglycosylase A, at 1,053,066 to 1,054,220
_RS05020
Position (kb)
1051
1052
1053 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1050.393 kb on + strand, within HGI48_RS05000 at 1050.393 kb on + strand, within HGI48_RS05000 at 1050.394 kb on - strand, within HGI48_RS05000 at 1050.394 kb on - strand, within HGI48_RS05000 at 1050.394 kb on - strand, within HGI48_RS05000 at 1050.394 kb on - strand, within HGI48_RS05000 at 1050.395 kb on + strand, within HGI48_RS05000 at 1050.395 kb on + strand, within HGI48_RS05000 at 1050.395 kb on + strand, within HGI48_RS05000 at 1050.395 kb on + strand at 1050.396 kb on - strand at 1050.396 kb on - strand, within HGI48_RS05000 at 1050.462 kb on + strand, within HGI48_RS05000 at 1050.462 kb on + strand, within HGI48_RS05000 at 1050.462 kb on + strand at 1050.463 kb on - strand, within HGI48_RS05000 at 1050.463 kb on - strand, within HGI48_RS05000 at 1050.567 kb on + strand at 1050.568 kb on - strand, within HGI48_RS05000 at 1050.568 kb on - strand, within HGI48_RS05000 at 1050.657 kb on + strand, within HGI48_RS05000 at 1050.683 kb on + strand, within HGI48_RS05000 at 1050.684 kb on - strand, within HGI48_RS05000 at 1050.768 kb on + strand, within HGI48_RS05000 at 1050.890 kb on + strand at 1050.935 kb on + strand, within HGI48_RS05000 at 1050.963 kb on + strand, within HGI48_RS05000 at 1050.963 kb on + strand, within HGI48_RS05000 at 1050.968 kb on - strand, within HGI48_RS05000 at 1051.067 kb on + strand at 1051.067 kb on + strand at 1051.067 kb on + strand at 1051.067 kb on + strand at 1051.068 kb on - strand at 1051.068 kb on - strand at 1051.068 kb on - strand at 1051.068 kb on - strand at 1051.068 kb on - strand at 1051.068 kb on - strand at 1051.068 kb on - strand at 1051.068 kb on - strand at 1051.104 kb on + strand at 1051.104 kb on + strand at 1051.104 kb on + strand at 1051.105 kb on - strand at 1051.107 kb on + strand at 1051.107 kb on + strand at 1051.107 kb on + strand at 1051.107 kb on + strand at 1051.107 kb on + strand at 1051.107 kb on + strand at 1051.107 kb on + strand at 1051.107 kb on + strand at 1051.107 kb on + strand at 1051.107 kb on + strand at 1051.107 kb on + strand at 1051.107 kb on + strand at 1051.108 kb on - strand at 1051.108 kb on - strand at 1051.108 kb on - strand at 1051.162 kb on + strand at 1051.211 kb on + strand at 1051.211 kb on + strand at 1051.217 kb on - strand at 1051.217 kb on - strand at 1051.217 kb on - strand at 1051.218 kb on - strand at 1051.230 kb on + strand at 1051.248 kb on - strand at 1051.381 kb on + strand at 1051.382 kb on - strand at 1051.385 kb on - strand at 1051.416 kb on - strand at 1051.483 kb on - strand, within HGI48_RS05005 at 1051.483 kb on - strand, within HGI48_RS05005 at 1051.483 kb on - strand, within HGI48_RS05005 at 1051.521 kb on + strand, within HGI48_RS05005 at 1051.521 kb on + strand, within HGI48_RS05005 at 1051.521 kb on + strand, within HGI48_RS05005 at 1051.521 kb on + strand, within HGI48_RS05005 at 1051.522 kb on - strand at 1051.522 kb on - strand, within HGI48_RS05005 at 1051.522 kb on - strand, within HGI48_RS05005 at 1051.670 kb on + strand, within HGI48_RS05005 at 1051.670 kb on + strand, within HGI48_RS05005 at 1051.671 kb on - strand, within HGI48_RS05005 at 1051.671 kb on - strand, within HGI48_RS05005 at 1051.693 kb on + strand, within HGI48_RS05005 at 1051.722 kb on - strand, within HGI48_RS05005 at 1051.788 kb on + strand, within HGI48_RS05005 at 1051.830 kb on + strand, within HGI48_RS05005 at 1051.830 kb on + strand, within HGI48_RS05005 at 1051.830 kb on + strand, within HGI48_RS05005 at 1051.830 kb on + strand at 1051.830 kb on + strand, within HGI48_RS05005 at 1051.830 kb on + strand, within HGI48_RS05005 at 1051.835 kb on + strand, within HGI48_RS05005 at 1051.835 kb on + strand, within HGI48_RS05005 at 1052.046 kb on + strand, within HGI48_RS05005 at 1052.046 kb on + strand at 1052.046 kb on + strand, within HGI48_RS05005 at 1052.046 kb on + strand, within HGI48_RS05005 at 1052.046 kb on + strand, within HGI48_RS05005 at 1052.046 kb on + strand, within HGI48_RS05005 at 1052.046 kb on + strand, within HGI48_RS05005 at 1052.047 kb on - strand, within HGI48_RS05005 at 1052.047 kb on - strand, within HGI48_RS05005 at 1052.047 kb on - strand, within HGI48_RS05005 at 1052.047 kb on - strand, within HGI48_RS05005 at 1052.086 kb on + strand, within HGI48_RS05005 at 1052.086 kb on + strand, within HGI48_RS05005 at 1052.086 kb on + strand, within HGI48_RS05005 at 1052.086 kb on + strand, within HGI48_RS05005 at 1052.442 kb on - strand, within HGI48_RS05005 at 1052.504 kb on + strand at 1052.505 kb on - strand at 1052.559 kb on + strand at 1052.581 kb on - strand at 1052.612 kb on + strand at 1052.612 kb on + strand at 1052.613 kb on - strand at 1052.633 kb on + strand at 1052.657 kb on + strand at 1052.658 kb on - strand at 1052.658 kb on - strand at 1052.759 kb on + strand at 1052.770 kb on + strand at 1052.770 kb on + strand at 1052.771 kb on - strand at 1052.771 kb on - strand at 1052.916 kb on + strand at 1052.917 kb on - strand at 1053.087 kb on + strand at 1053.087 kb on + strand at 1053.087 kb on + strand at 1053.087 kb on + strand at 1053.088 kb on - strand at 1053.088 kb on - strand at 1053.088 kb on - strand at 1053.088 kb on - strand at 1053.088 kb on - strand at 1053.088 kb on - strand at 1053.159 kb on + strand at 1053.159 kb on + strand at 1053.160 kb on - strand at 1053.160 kb on - strand at 1053.160 kb on - strand at 1053.161 kb on + strand at 1053.161 kb on + strand at 1053.161 kb on + strand at 1053.161 kb on + strand at 1053.161 kb on + strand at 1053.162 kb on - strand at 1053.162 kb on - strand at 1053.162 kb on - strand at 1053.162 kb on - strand at 1053.162 kb on - strand at 1053.162 kb on - strand at 1053.162 kb on - strand at 1053.162 kb on - strand at 1053.162 kb on - strand at 1053.162 kb on - strand at 1053.162 kb on - strand at 1053.285 kb on + strand, within HGI48_RS05020 at 1053.429 kb on + strand, within HGI48_RS05020 at 1053.429 kb on + strand, within HGI48_RS05020 at 1053.429 kb on + strand at 1053.429 kb on + strand, within HGI48_RS05020 at 1053.429 kb on + strand, within HGI48_RS05020 at 1053.429 kb on + strand, within HGI48_RS05020 at 1053.430 kb on - strand, within HGI48_RS05020 at 1053.430 kb on - strand, within HGI48_RS05020 at 1053.430 kb on - strand, within HGI48_RS05020 at 1053.430 kb on - strand, within HGI48_RS05020 at 1053.486 kb on + strand, within HGI48_RS05020 at 1053.486 kb on + strand, within HGI48_RS05020 at 1053.486 kb on + strand, within HGI48_RS05020 at 1053.487 kb on - strand, within HGI48_RS05020 at 1053.487 kb on - strand, within HGI48_RS05020 at 1053.487 kb on - strand, within HGI48_RS05020 at 1053.541 kb on - strand, within HGI48_RS05020
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 1,050,393 + HGI48_RS05000 0.45 -1.0 1,050,393 + HGI48_RS05000 0.45 +1.3 1,050,394 - HGI48_RS05000 0.45 -1.2 1,050,394 - HGI48_RS05000 0.45 -1.5 1,050,394 - HGI48_RS05000 0.45 +0.0 1,050,394 - HGI48_RS05000 0.45 +0.7 1,050,395 + HGI48_RS05000 0.45 +2.0 1,050,395 + HGI48_RS05000 0.45 -1.0 1,050,395 + HGI48_RS05000 0.45 -2.7 1,050,395 + +0.8 1,050,396 - -0.7 1,050,396 - HGI48_RS05000 0.45 +1.2 1,050,462 + HGI48_RS05000 0.50 -2.2 1,050,462 + HGI48_RS05000 0.50 +0.5 1,050,462 + -0.7 1,050,463 - HGI48_RS05000 0.50 +0.1 1,050,463 - HGI48_RS05000 0.50 -0.4 1,050,567 + +1.3 1,050,568 - HGI48_RS05000 0.58 +1.4 1,050,568 - HGI48_RS05000 0.58 -0.2 1,050,657 + HGI48_RS05000 0.64 +1.1 1,050,683 + HGI48_RS05000 0.66 -1.6 1,050,684 - HGI48_RS05000 0.66 -1.2 1,050,768 + HGI48_RS05000 0.72 -2.2 1,050,890 + -0.2 1,050,935 + HGI48_RS05000 0.85 -2.0 1,050,963 + HGI48_RS05000 0.87 -0.9 1,050,963 + HGI48_RS05000 0.87 -1.5 1,050,968 - HGI48_RS05000 0.87 -2.0 1,051,067 + -1.2 1,051,067 + -1.5 1,051,067 + -0.9 1,051,067 + -0.6 1,051,068 - -0.6 1,051,068 - -0.7 1,051,068 - -0.9 1,051,068 - +0.6 1,051,068 - +0.3 1,051,068 - +0.0 1,051,068 - -0.5 1,051,068 - -2.5 1,051,104 + -1.5 1,051,104 + +0.4 1,051,104 + +1.0 1,051,105 - -1.0 1,051,107 + +0.2 1,051,107 + -2.7 1,051,107 + -0.7 1,051,107 + -0.6 1,051,107 + +1.2 1,051,107 + -0.7 1,051,107 + -0.2 1,051,107 + -1.0 1,051,107 + -1.2 1,051,107 + -1.0 1,051,107 + -1.2 1,051,107 + -2.7 1,051,108 - -1.1 1,051,108 - +0.3 1,051,108 - +0.3 1,051,162 + +1.3 1,051,211 + +0.8 1,051,211 + -0.1 1,051,217 - -0.1 1,051,217 - -0.5 1,051,217 - -1.0 1,051,218 - -1.5 1,051,230 + -0.2 1,051,248 - +1.3 1,051,381 + -1.2 1,051,382 - -0.9 1,051,385 - -0.2 1,051,416 - +0.5 1,051,483 - HGI48_RS05005 0.11 -1.5 1,051,483 - HGI48_RS05005 0.11 -1.5 1,051,483 - HGI48_RS05005 0.11 -0.2 1,051,521 + HGI48_RS05005 0.14 -0.6 1,051,521 + HGI48_RS05005 0.14 +0.8 1,051,521 + HGI48_RS05005 0.14 -0.2 1,051,521 + HGI48_RS05005 0.14 -0.5 1,051,522 - -0.2 1,051,522 - HGI48_RS05005 0.14 -1.0 1,051,522 - HGI48_RS05005 0.14 -0.6 1,051,670 + HGI48_RS05005 0.26 -1.2 1,051,670 + HGI48_RS05005 0.26 +0.0 1,051,671 - HGI48_RS05005 0.26 -1.3 1,051,671 - HGI48_RS05005 0.26 -0.4 1,051,693 + HGI48_RS05005 0.28 -1.2 1,051,722 - HGI48_RS05005 0.31 +1.2 1,051,788 + HGI48_RS05005 0.36 +0.5 1,051,830 + HGI48_RS05005 0.39 -1.3 1,051,830 + HGI48_RS05005 0.39 -0.8 1,051,830 + HGI48_RS05005 0.39 +0.1 1,051,830 + -0.2 1,051,830 + HGI48_RS05005 0.39 +1.4 1,051,830 + HGI48_RS05005 0.39 -1.7 1,051,835 + HGI48_RS05005 0.40 +0.3 1,051,835 + HGI48_RS05005 0.40 +2.4 1,052,046 + HGI48_RS05005 0.57 -1.7 1,052,046 + -0.7 1,052,046 + HGI48_RS05005 0.57 -0.2 1,052,046 + HGI48_RS05005 0.57 +0.4 1,052,046 + HGI48_RS05005 0.57 +0.1 1,052,046 + HGI48_RS05005 0.57 +0.7 1,052,046 + HGI48_RS05005 0.57 -2.2 1,052,047 - HGI48_RS05005 0.57 +0.0 1,052,047 - HGI48_RS05005 0.57 +0.7 1,052,047 - HGI48_RS05005 0.57 +0.8 1,052,047 - HGI48_RS05005 0.57 -0.5 1,052,086 + HGI48_RS05005 0.60 +0.8 1,052,086 + HGI48_RS05005 0.60 -0.4 1,052,086 + HGI48_RS05005 0.60 -0.2 1,052,086 + HGI48_RS05005 0.60 -1.2 1,052,442 - HGI48_RS05005 0.89 +1.0 1,052,504 + +1.7 1,052,505 - +0.8 1,052,559 + +2.3 1,052,581 - +1.4 1,052,612 + -0.2 1,052,612 + -0.2 1,052,613 - -0.1 1,052,633 + -0.2 1,052,657 + +0.9 1,052,658 - -1.7 1,052,658 - -1.0 1,052,759 + -2.0 1,052,770 + +0.8 1,052,770 + -0.5 1,052,771 - -1.0 1,052,771 - +1.0 1,052,916 + +1.5 1,052,917 - -0.0 1,053,087 + +1.6 1,053,087 + -1.2 1,053,087 + -2.2 1,053,087 + +0.7 1,053,088 - -2.0 1,053,088 - -0.2 1,053,088 - -1.2 1,053,088 - -2.0 1,053,088 - -1.4 1,053,088 - -0.8 1,053,159 + -0.2 1,053,159 + -1.5 1,053,160 - -0.9 1,053,160 - -0.2 1,053,160 - +0.2 1,053,161 + -2.0 1,053,161 + +0.5 1,053,161 + -0.3 1,053,161 + -1.5 1,053,161 + +0.8 1,053,162 - +1.1 1,053,162 - +2.2 1,053,162 - +2.7 1,053,162 - -0.9 1,053,162 - -1.8 1,053,162 - -1.5 1,053,162 - -0.8 1,053,162 - -0.4 1,053,162 - +0.8 1,053,162 - +1.0 1,053,162 - -2.0 1,053,285 + HGI48_RS05020 0.19 -1.4 1,053,429 + HGI48_RS05020 0.31 +0.3 1,053,429 + HGI48_RS05020 0.31 +0.6 1,053,429 + -0.2 1,053,429 + HGI48_RS05020 0.31 -0.5 1,053,429 + HGI48_RS05020 0.31 -2.6 1,053,429 + HGI48_RS05020 0.31 -2.0 1,053,430 - HGI48_RS05020 0.32 +0.2 1,053,430 - HGI48_RS05020 0.32 -0.7 1,053,430 - HGI48_RS05020 0.32 -2.9 1,053,430 - HGI48_RS05020 0.32 +0.4 1,053,486 + HGI48_RS05020 0.36 -0.8 1,053,486 + HGI48_RS05020 0.36 +1.0 1,053,486 + HGI48_RS05020 0.36 -0.2 1,053,487 - HGI48_RS05020 0.36 -2.5 1,053,487 - HGI48_RS05020 0.36 +1.2 1,053,487 - HGI48_RS05020 0.36 -1.3 1,053,541 - HGI48_RS05020 0.41 +0.1
Or see this region's nucleotide sequence