Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS04680 and HGI48_RS04685 are separated by 70 nucleotides
HGI48_RS04680: HGI48_RS04680 - Expansin-YoaJ, at 984,842 to 985,567
_RS04680
HGI48_RS04685: HGI48_RS04685 - glycoside hydrolase family 5 protein, at 985,638 to 986,585
_RS04685
Position (kb) 984
985
986 Strain fitness (log2 ratio) -2
-1
0
1
2 at 983.896 kb on + strand at 983.896 kb on + strand at 983.897 kb on - strand at 983.897 kb on - strand at 983.922 kb on + strand at 983.934 kb on + strand at 983.958 kb on + strand at 983.986 kb on - strand at 983.996 kb on + strand at 983.997 kb on - strand at 984.059 kb on + strand at 984.059 kb on + strand at 984.059 kb on + strand at 984.060 kb on - strand at 984.060 kb on - strand at 984.060 kb on - strand at 984.115 kb on + strand at 984.115 kb on + strand at 984.116 kb on - strand at 984.116 kb on - strand at 984.118 kb on + strand at 984.118 kb on + strand at 984.118 kb on + strand at 984.119 kb on - strand at 984.119 kb on - strand at 984.127 kb on - strand at 984.183 kb on + strand at 984.184 kb on - strand at 984.201 kb on + strand at 984.201 kb on + strand at 984.240 kb on + strand at 984.243 kb on + strand at 984.355 kb on + strand at 984.356 kb on - strand at 984.356 kb on - strand at 984.366 kb on + strand at 984.366 kb on + strand at 984.367 kb on - strand at 984.367 kb on - strand at 984.367 kb on - strand at 984.367 kb on - strand at 984.381 kb on - strand at 984.383 kb on - strand at 984.383 kb on - strand at 984.410 kb on - strand at 984.452 kb on + strand at 984.453 kb on - strand at 984.453 kb on - strand at 984.453 kb on - strand at 984.453 kb on - strand at 984.501 kb on + strand at 984.507 kb on + strand at 984.507 kb on + strand at 984.507 kb on + strand at 984.507 kb on + strand at 984.507 kb on + strand at 984.514 kb on + strand at 984.587 kb on + strand at 984.588 kb on - strand at 984.727 kb on - strand at 984.727 kb on - strand at 984.727 kb on - strand at 984.732 kb on + strand at 984.732 kb on + strand at 984.732 kb on + strand at 984.732 kb on + strand at 984.732 kb on + strand at 984.733 kb on - strand at 984.795 kb on + strand at 984.821 kb on + strand at 984.859 kb on - strand at 984.879 kb on + strand at 984.907 kb on + strand at 984.907 kb on + strand at 984.908 kb on - strand at 984.908 kb on - strand at 984.908 kb on - strand at 984.932 kb on - strand, within HGI48_RS04680 at 984.932 kb on - strand, within HGI48_RS04680 at 984.951 kb on + strand, within HGI48_RS04680 at 984.952 kb on - strand, within HGI48_RS04680 at 984.952 kb on - strand, within HGI48_RS04680 at 984.952 kb on - strand, within HGI48_RS04680 at 984.952 kb on - strand, within HGI48_RS04680 at 985.005 kb on + strand, within HGI48_RS04680 at 985.005 kb on + strand, within HGI48_RS04680 at 985.006 kb on - strand, within HGI48_RS04680 at 985.006 kb on - strand, within HGI48_RS04680 at 985.009 kb on - strand, within HGI48_RS04680 at 985.009 kb on - strand, within HGI48_RS04680 at 985.030 kb on + strand, within HGI48_RS04680 at 985.031 kb on - strand at 985.031 kb on - strand, within HGI48_RS04680 at 985.139 kb on + strand, within HGI48_RS04680 at 985.139 kb on + strand, within HGI48_RS04680 at 985.140 kb on - strand, within HGI48_RS04680 at 985.140 kb on - strand at 985.140 kb on - strand, within HGI48_RS04680 at 985.140 kb on - strand, within HGI48_RS04680 at 985.154 kb on + strand, within HGI48_RS04680 at 985.160 kb on - strand, within HGI48_RS04680 at 985.183 kb on + strand, within HGI48_RS04680 at 985.183 kb on + strand at 985.183 kb on + strand, within HGI48_RS04680 at 985.183 kb on + strand, within HGI48_RS04680 at 985.184 kb on - strand, within HGI48_RS04680 at 985.233 kb on + strand, within HGI48_RS04680 at 985.234 kb on - strand, within HGI48_RS04680 at 985.234 kb on - strand, within HGI48_RS04680 at 985.234 kb on - strand, within HGI48_RS04680 at 985.289 kb on - strand, within HGI48_RS04680 at 985.290 kb on + strand, within HGI48_RS04680 at 985.343 kb on + strand, within HGI48_RS04680 at 985.358 kb on - strand at 985.365 kb on - strand at 985.368 kb on - strand, within HGI48_RS04680 at 985.407 kb on - strand, within HGI48_RS04680 at 985.446 kb on + strand, within HGI48_RS04680 at 985.446 kb on + strand, within HGI48_RS04680 at 985.446 kb on + strand, within HGI48_RS04680 at 985.447 kb on - strand, within HGI48_RS04680 at 985.447 kb on - strand, within HGI48_RS04680 at 985.484 kb on + strand, within HGI48_RS04680 at 985.485 kb on - strand, within HGI48_RS04680 at 985.490 kb on + strand, within HGI48_RS04680 at 985.490 kb on + strand, within HGI48_RS04680 at 985.491 kb on - strand, within HGI48_RS04680 at 985.491 kb on - strand, within HGI48_RS04680 at 985.493 kb on - strand, within HGI48_RS04680 at 985.571 kb on - strand at 985.629 kb on + strand at 985.683 kb on - strand at 985.685 kb on + strand at 985.686 kb on - strand at 985.707 kb on - strand at 985.739 kb on + strand at 985.754 kb on + strand at 985.766 kb on + strand, within HGI48_RS04685 at 985.826 kb on + strand, within HGI48_RS04685 at 985.838 kb on + strand, within HGI48_RS04685 at 985.839 kb on - strand, within HGI48_RS04685 at 985.850 kb on - strand at 985.935 kb on - strand, within HGI48_RS04685 at 985.935 kb on - strand, within HGI48_RS04685 at 985.969 kb on + strand, within HGI48_RS04685 at 985.983 kb on + strand, within HGI48_RS04685 at 985.983 kb on + strand, within HGI48_RS04685 at 985.983 kb on + strand, within HGI48_RS04685 at 985.983 kb on + strand, within HGI48_RS04685 at 985.983 kb on + strand, within HGI48_RS04685 at 985.983 kb on + strand, within HGI48_RS04685 at 985.983 kb on + strand, within HGI48_RS04685 at 985.984 kb on - strand at 985.984 kb on - strand, within HGI48_RS04685 at 985.984 kb on - strand, within HGI48_RS04685 at 985.990 kb on + strand at 985.991 kb on - strand, within HGI48_RS04685 at 985.991 kb on - strand, within HGI48_RS04685 at 986.091 kb on + strand, within HGI48_RS04685 at 986.091 kb on + strand at 986.091 kb on + strand, within HGI48_RS04685 at 986.091 kb on + strand, within HGI48_RS04685 at 986.100 kb on + strand, within HGI48_RS04685 at 986.100 kb on + strand, within HGI48_RS04685 at 986.101 kb on - strand, within HGI48_RS04685 at 986.101 kb on - strand, within HGI48_RS04685 at 986.146 kb on - strand, within HGI48_RS04685 at 986.274 kb on + strand at 986.275 kb on - strand, within HGI48_RS04685 at 986.289 kb on + strand, within HGI48_RS04685 at 986.339 kb on - strand, within HGI48_RS04685 at 986.444 kb on + strand, within HGI48_RS04685 at 986.445 kb on - strand, within HGI48_RS04685 at 986.445 kb on - strand, within HGI48_RS04685 at 986.448 kb on + strand, within HGI48_RS04685 at 986.448 kb on + strand, within HGI48_RS04685 at 986.448 kb on + strand, within HGI48_RS04685 at 986.448 kb on + strand, within HGI48_RS04685 at 986.448 kb on + strand, within HGI48_RS04685 at 986.448 kb on + strand, within HGI48_RS04685 at 986.448 kb on + strand, within HGI48_RS04685 at 986.448 kb on + strand, within HGI48_RS04685 at 986.449 kb on - strand at 986.449 kb on - strand, within HGI48_RS04685 at 986.449 kb on - strand, within HGI48_RS04685 at 986.449 kb on - strand, within HGI48_RS04685 at 986.449 kb on - strand, within HGI48_RS04685 at 986.450 kb on + strand, within HGI48_RS04685 at 986.450 kb on + strand, within HGI48_RS04685 at 986.450 kb on + strand, within HGI48_RS04685 at 986.450 kb on + strand, within HGI48_RS04685 at 986.450 kb on + strand, within HGI48_RS04685 at 986.450 kb on + strand, within HGI48_RS04685 at 986.451 kb on - strand at 986.451 kb on - strand, within HGI48_RS04685 at 986.451 kb on - strand, within HGI48_RS04685 at 986.451 kb on - strand, within HGI48_RS04685 at 986.451 kb on - strand at 986.451 kb on - strand, within HGI48_RS04685 at 986.460 kb on + strand, within HGI48_RS04685 at 986.465 kb on - strand, within HGI48_RS04685 at 986.475 kb on + strand, within HGI48_RS04685 at 986.476 kb on - strand, within HGI48_RS04685 at 986.476 kb on - strand, within HGI48_RS04685 at 986.523 kb on - strand at 986.553 kb on - strand at 986.556 kb on - strand at 986.556 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 983,896 + +0.3 983,896 + -1.1 983,897 - +0.2 983,897 - -0.1 983,922 + +0.7 983,934 + +0.7 983,958 + -0.7 983,986 - +1.4 983,996 + -0.1 983,997 - +0.9 984,059 + +0.4 984,059 + -0.4 984,059 + +0.7 984,060 - -1.1 984,060 - +1.5 984,060 - -0.6 984,115 + -0.7 984,115 + +0.7 984,116 - +0.4 984,116 - -0.1 984,118 + -1.1 984,118 + -1.1 984,118 + +1.2 984,119 - +2.2 984,119 - -0.7 984,127 - -0.3 984,183 + -1.7 984,184 - +1.5 984,201 + +0.9 984,201 + +0.6 984,240 + -0.7 984,243 + -1.4 984,355 + +0.3 984,356 - +1.4 984,356 - -0.1 984,366 + +1.6 984,366 + +0.6 984,367 - -0.3 984,367 - -1.1 984,367 - +1.1 984,367 - -1.9 984,381 - +0.3 984,383 - -2.1 984,383 - -0.0 984,410 - +0.1 984,452 + +0.9 984,453 - -2.6 984,453 - +0.1 984,453 - +1.5 984,453 - -0.7 984,501 + +0.5 984,507 + -2.3 984,507 + +0.6 984,507 + -0.3 984,507 + -1.0 984,507 + +0.0 984,514 + +0.9 984,587 + -0.7 984,588 - -0.7 984,727 - -1.1 984,727 - -1.4 984,727 - -0.5 984,732 + -1.4 984,732 + +0.7 984,732 + -0.2 984,732 + -0.4 984,732 + -0.1 984,733 - -0.4 984,795 + -1.4 984,821 + -1.0 984,859 - +0.9 984,879 + +2.0 984,907 + +0.5 984,907 + -1.1 984,908 - -0.1 984,908 - -1.3 984,908 - -1.1 984,932 - HGI48_RS04680 0.12 -0.2 984,932 - HGI48_RS04680 0.12 -1.1 984,951 + HGI48_RS04680 0.15 +0.7 984,952 - HGI48_RS04680 0.15 -0.7 984,952 - HGI48_RS04680 0.15 -0.7 984,952 - HGI48_RS04680 0.15 -1.1 984,952 - HGI48_RS04680 0.15 +2.6 985,005 + HGI48_RS04680 0.22 +0.1 985,005 + HGI48_RS04680 0.22 +0.1 985,006 - HGI48_RS04680 0.23 -1.4 985,006 - HGI48_RS04680 0.23 +0.4 985,009 - HGI48_RS04680 0.23 +0.3 985,009 - HGI48_RS04680 0.23 -0.2 985,030 + HGI48_RS04680 0.26 +0.5 985,031 - +1.5 985,031 - HGI48_RS04680 0.26 -1.9 985,139 + HGI48_RS04680 0.41 -1.4 985,139 + HGI48_RS04680 0.41 +1.1 985,140 - HGI48_RS04680 0.41 +2.3 985,140 - +1.3 985,140 - HGI48_RS04680 0.41 +1.3 985,140 - HGI48_RS04680 0.41 +1.2 985,154 + HGI48_RS04680 0.43 +0.9 985,160 - HGI48_RS04680 0.44 +0.7 985,183 + HGI48_RS04680 0.47 -0.1 985,183 + -0.7 985,183 + HGI48_RS04680 0.47 +0.1 985,183 + HGI48_RS04680 0.47 +0.5 985,184 - HGI48_RS04680 0.47 -1.1 985,233 + HGI48_RS04680 0.54 +2.0 985,234 - HGI48_RS04680 0.54 +1.7 985,234 - HGI48_RS04680 0.54 +0.1 985,234 - HGI48_RS04680 0.54 -0.4 985,289 - HGI48_RS04680 0.62 -0.3 985,290 + HGI48_RS04680 0.62 +1.7 985,343 + HGI48_RS04680 0.69 -0.0 985,358 - +2.1 985,365 - -0.1 985,368 - HGI48_RS04680 0.72 +0.4 985,407 - HGI48_RS04680 0.78 +1.4 985,446 + HGI48_RS04680 0.83 -0.6 985,446 + HGI48_RS04680 0.83 -0.8 985,446 + HGI48_RS04680 0.83 +0.1 985,447 - HGI48_RS04680 0.83 +1.0 985,447 - HGI48_RS04680 0.83 +0.4 985,484 + HGI48_RS04680 0.88 -1.7 985,485 - HGI48_RS04680 0.89 +0.6 985,490 + HGI48_RS04680 0.89 -0.5 985,490 + HGI48_RS04680 0.89 -1.9 985,491 - HGI48_RS04680 0.89 +1.5 985,491 - HGI48_RS04680 0.89 -0.1 985,493 - HGI48_RS04680 0.90 -1.7 985,571 - -0.3 985,629 + -1.9 985,683 - +1.5 985,685 + -0.9 985,686 - +1.6 985,707 - +0.9 985,739 + +0.9 985,754 + -0.1 985,766 + HGI48_RS04685 0.14 -1.7 985,826 + HGI48_RS04685 0.20 -0.7 985,838 + HGI48_RS04685 0.21 +0.5 985,839 - HGI48_RS04685 0.21 +1.0 985,850 - -0.1 985,935 - HGI48_RS04685 0.31 -0.3 985,935 - HGI48_RS04685 0.31 +2.3 985,969 + HGI48_RS04685 0.35 +0.2 985,983 + HGI48_RS04685 0.36 -0.3 985,983 + HGI48_RS04685 0.36 +1.1 985,983 + HGI48_RS04685 0.36 +1.3 985,983 + HGI48_RS04685 0.36 -2.1 985,983 + HGI48_RS04685 0.36 +0.7 985,983 + HGI48_RS04685 0.36 -0.6 985,983 + HGI48_RS04685 0.36 -0.1 985,984 - +0.9 985,984 - HGI48_RS04685 0.36 -1.7 985,984 - HGI48_RS04685 0.36 -2.7 985,990 + -0.7 985,991 - HGI48_RS04685 0.37 +1.1 985,991 - HGI48_RS04685 0.37 -1.7 986,091 + HGI48_RS04685 0.48 -0.7 986,091 + +2.2 986,091 + HGI48_RS04685 0.48 -0.5 986,091 + HGI48_RS04685 0.48 +0.9 986,100 + HGI48_RS04685 0.49 +0.6 986,100 + HGI48_RS04685 0.49 -0.1 986,101 - HGI48_RS04685 0.49 -0.1 986,101 - HGI48_RS04685 0.49 +0.5 986,146 - HGI48_RS04685 0.54 -0.7 986,274 + +0.9 986,275 - HGI48_RS04685 0.67 -0.1 986,289 + HGI48_RS04685 0.69 +0.6 986,339 - HGI48_RS04685 0.74 -1.4 986,444 + HGI48_RS04685 0.85 +0.1 986,445 - HGI48_RS04685 0.85 +1.1 986,445 - HGI48_RS04685 0.85 -0.0 986,448 + HGI48_RS04685 0.85 +0.5 986,448 + HGI48_RS04685 0.85 -1.7 986,448 + HGI48_RS04685 0.85 -1.2 986,448 + HGI48_RS04685 0.85 +2.0 986,448 + HGI48_RS04685 0.85 -0.5 986,448 + HGI48_RS04685 0.85 -1.5 986,448 + HGI48_RS04685 0.85 +0.5 986,448 + HGI48_RS04685 0.85 -1.1 986,449 - +0.9 986,449 - HGI48_RS04685 0.86 -0.4 986,449 - HGI48_RS04685 0.86 -0.9 986,449 - HGI48_RS04685 0.86 +0.1 986,449 - HGI48_RS04685 0.86 +1.9 986,450 + HGI48_RS04685 0.86 +1.2 986,450 + HGI48_RS04685 0.86 +0.9 986,450 + HGI48_RS04685 0.86 +0.6 986,450 + HGI48_RS04685 0.86 +1.7 986,450 + HGI48_RS04685 0.86 -0.8 986,450 + HGI48_RS04685 0.86 -0.2 986,451 - +0.9 986,451 - HGI48_RS04685 0.86 -1.5 986,451 - HGI48_RS04685 0.86 -0.9 986,451 - HGI48_RS04685 0.86 -1.9 986,451 - +2.5 986,451 - HGI48_RS04685 0.86 +0.2 986,460 + HGI48_RS04685 0.87 -1.1 986,465 - HGI48_RS04685 0.87 -1.5 986,475 + HGI48_RS04685 0.88 +0.3 986,476 - HGI48_RS04685 0.88 +1.2 986,476 - HGI48_RS04685 0.88 -1.5 986,523 - +1.5 986,553 - -1.5 986,556 - +0.9 986,556 - -1.9
Or see this region's nucleotide sequence