Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS03190 and HGI48_RS03195 are separated by 158 nucleotides HGI48_RS03195 and HGI48_RS03200 are separated by 47 nucleotides HGI48_RS03200 and HGI48_RS03205 are separated by 105 nucleotides HGI48_RS03205 and HGI48_RS03210 are separated by 8 nucleotides
HGI48_RS03190: HGI48_RS03190 - ribosome assembly RNA-binding protein YhbY, at 674,701 to 674,994
_RS03190
HGI48_RS03195: HGI48_RS03195 - 23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE, at 675,153 to 675,782
_RS03195
HGI48_RS03200: HGI48_RS03200 - ATP-dependent zinc metalloprotease FtsH, at 675,830 to 677,782
_RS03200
HGI48_RS03205: HGI48_RS03205 - dihydropteroate synthase, at 677,888 to 678,721
_RS03205
HGI48_RS03210: HGI48_RS03210 - phosphoglucosamine mutase, at 678,730 to 680,067
_RS03210
Position (kb) 675
676
677
678 Strain fitness (log2 ratio) -2
-1
0
1
2 at 674.893 kb on + strand at 674.893 kb on + strand, within HGI48_RS03190 at 674.893 kb on + strand, within HGI48_RS03190 at 674.894 kb on - strand, within HGI48_RS03190 at 674.894 kb on - strand, within HGI48_RS03190 at 674.894 kb on - strand, within HGI48_RS03190 at 674.928 kb on + strand, within HGI48_RS03190 at 674.929 kb on - strand, within HGI48_RS03190 at 675.113 kb on + strand at 675.113 kb on + strand at 675.113 kb on + strand at 675.113 kb on + strand at 675.113 kb on + strand at 675.113 kb on + strand at 675.113 kb on + strand at 675.113 kb on + strand at 675.113 kb on + strand at 675.113 kb on + strand at 675.114 kb on - strand at 675.117 kb on + strand at 675.117 kb on + strand at 675.117 kb on + strand at 675.123 kb on + strand at 675.123 kb on + strand at 675.123 kb on + strand at 675.123 kb on + strand at 675.123 kb on + strand at 675.124 kb on - strand at 675.780 kb on + strand at 675.780 kb on + strand at 677.734 kb on + strand at 677.734 kb on + strand at 677.735 kb on - strand at 677.752 kb on + strand at 677.752 kb on + strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.781 kb on - strand at 677.781 kb on - strand at 677.781 kb on - strand at 677.781 kb on - strand at 677.781 kb on - strand at 677.781 kb on - strand at 677.859 kb on - strand at 677.875 kb on + strand at 677.875 kb on + strand at 678.348 kb on - strand, within HGI48_RS03205 at 678.392 kb on - strand, within HGI48_RS03205 at 678.392 kb on - strand, within HGI48_RS03205 at 678.468 kb on + strand, within HGI48_RS03205 at 678.475 kb on + strand, within HGI48_RS03205 at 678.476 kb on - strand, within HGI48_RS03205 at 678.684 kb on + strand at 678.684 kb on + strand at 678.684 kb on + strand at 678.685 kb on - strand at 678.685 kb on - strand at 678.685 kb on - strand at 678.685 kb on - strand at 678.685 kb on - strand at 678.685 kb on - strand at 678.719 kb on + strand at 678.729 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 674,893 + -0.6 674,893 + HGI48_RS03190 0.65 -0.0 674,893 + HGI48_RS03190 0.65 +0.7 674,894 - HGI48_RS03190 0.66 -1.0 674,894 - HGI48_RS03190 0.66 -1.3 674,894 - HGI48_RS03190 0.66 -0.2 674,928 + HGI48_RS03190 0.77 -1.0 674,929 - HGI48_RS03190 0.78 -1.5 675,113 + -1.0 675,113 + -0.4 675,113 + +1.2 675,113 + -0.7 675,113 + -1.6 675,113 + +0.4 675,113 + -1.7 675,113 + -0.6 675,113 + +1.2 675,113 + -0.2 675,114 - +0.2 675,117 + +1.0 675,117 + +0.4 675,117 + -0.1 675,123 + +2.6 675,123 + -0.0 675,123 + +0.2 675,123 + -1.0 675,123 + +0.7 675,124 - -1.0 675,780 + +1.2 675,780 + -2.0 677,734 + +0.7 677,734 + -0.0 677,735 - -0.9 677,752 + -0.3 677,752 + -0.3 677,753 - -2.2 677,753 - -0.6 677,753 - -1.7 677,753 - +0.3 677,753 - +1.7 677,753 - +0.7 677,753 - -1.6 677,780 + +1.0 677,780 + -0.8 677,780 + -1.2 677,780 + -1.0 677,780 + +1.6 677,780 + +0.4 677,780 + +0.8 677,780 + -0.0 677,780 + +1.3 677,781 - -1.3 677,781 - -0.0 677,781 - -0.0 677,781 - -0.6 677,781 - +0.0 677,781 - +0.4 677,859 - -0.0 677,875 + +0.2 677,875 + -0.6 678,348 - HGI48_RS03205 0.55 +0.7 678,392 - HGI48_RS03205 0.60 -2.0 678,392 - HGI48_RS03205 0.60 +1.0 678,468 + HGI48_RS03205 0.70 +1.0 678,475 + HGI48_RS03205 0.70 +1.3 678,476 - HGI48_RS03205 0.71 -0.0 678,684 + -2.7 678,684 + -1.3 678,684 + -0.2 678,685 - -0.3 678,685 - -1.3 678,685 - -2.3 678,685 - +1.3 678,685 - -2.6 678,685 - -1.6 678,719 + -0.4 678,729 + -1.0
Or see this region's nucleotide sequence