Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS02235 and HGI48_RS02240 are separated by 58 nucleotides HGI48_RS02240 and HGI48_RS02245 are separated by 51 nucleotides HGI48_RS02245 and HGI48_RS02250 are separated by 55 nucleotides HGI48_RS02250 and HGI48_RS02255 overlap by 12 nucleotides HGI48_RS02255 and HGI48_RS02260 are separated by 387 nucleotides
HGI48_RS02235: HGI48_RS02235 - IS481 family transposase, at 487,372 to 488,414
_RS02235
HGI48_RS02240: HGI48_RS02240 - DUF4276 family protein, at 488,473 to 488,619
_RS02240
HGI48_RS02245: HGI48_RS02245 - Lrp/AsnC ligand binding domain-containing protein, at 488,671 to 489,174
_RS02245
HGI48_RS02250: HGI48_RS02250 - hypothetical protein, at 489,230 to 489,367
_RS02250
HGI48_RS02255: HGI48_RS02255 - chorismate mutase, at 489,356 to 489,886
_RS02255
HGI48_RS02260: HGI48_RS02260 - MFS transporter, at 490,274 to 491,506
_RS02260
Position (kb) 489
490 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 488.430 kb on + strand at 488.432 kb on + strand at 488.466 kb on + strand at 488.466 kb on + strand at 488.469 kb on + strand at 488.469 kb on + strand at 488.469 kb on + strand at 488.475 kb on + strand at 488.475 kb on + strand at 488.475 kb on + strand at 488.475 kb on + strand at 488.475 kb on + strand at 488.475 kb on + strand at 488.476 kb on - strand at 488.476 kb on - strand at 488.476 kb on - strand at 488.476 kb on - strand at 488.476 kb on - strand at 488.481 kb on + strand at 488.482 kb on - strand at 488.503 kb on + strand, within HGI48_RS02240 at 488.503 kb on + strand, within HGI48_RS02240 at 488.504 kb on - strand, within HGI48_RS02240 at 488.504 kb on - strand, within HGI48_RS02240 at 488.517 kb on - strand, within HGI48_RS02240 at 488.541 kb on + strand, within HGI48_RS02240 at 488.553 kb on + strand, within HGI48_RS02240 at 488.557 kb on - strand, within HGI48_RS02240 at 488.557 kb on - strand, within HGI48_RS02240 at 488.557 kb on - strand, within HGI48_RS02240 at 488.617 kb on + strand at 488.618 kb on - strand at 488.618 kb on - strand at 488.637 kb on + strand at 488.637 kb on + strand at 488.638 kb on - strand at 488.644 kb on - strand at 488.734 kb on + strand, within HGI48_RS02245 at 488.813 kb on + strand, within HGI48_RS02245 at 488.813 kb on + strand, within HGI48_RS02245 at 488.975 kb on + strand at 488.992 kb on + strand, within HGI48_RS02245 at 488.992 kb on + strand, within HGI48_RS02245 at 488.993 kb on - strand, within HGI48_RS02245 at 489.092 kb on + strand, within HGI48_RS02245 at 489.093 kb on - strand, within HGI48_RS02245 at 489.201 kb on + strand at 489.201 kb on + strand at 489.202 kb on - strand at 489.202 kb on - strand at 489.202 kb on - strand at 489.209 kb on - strand at 489.262 kb on + strand, within HGI48_RS02250 at 489.262 kb on + strand, within HGI48_RS02250 at 489.263 kb on - strand, within HGI48_RS02250 at 489.263 kb on - strand, within HGI48_RS02250 at 489.265 kb on + strand, within HGI48_RS02250 at 489.265 kb on + strand, within HGI48_RS02250 at 489.265 kb on + strand, within HGI48_RS02250 at 489.266 kb on - strand, within HGI48_RS02250 at 489.272 kb on + strand, within HGI48_RS02250 at 489.273 kb on - strand, within HGI48_RS02250 at 489.273 kb on - strand, within HGI48_RS02250 at 489.322 kb on + strand, within HGI48_RS02250 at 489.322 kb on + strand, within HGI48_RS02250 at 489.323 kb on - strand, within HGI48_RS02250 at 489.358 kb on - strand at 489.358 kb on - strand at 489.358 kb on - strand at 489.358 kb on - strand at 489.365 kb on + strand at 489.366 kb on - strand at 489.440 kb on + strand, within HGI48_RS02255 at 489.440 kb on + strand, within HGI48_RS02255 at 489.440 kb on + strand, within HGI48_RS02255 at 489.441 kb on - strand, within HGI48_RS02255 at 489.441 kb on - strand, within HGI48_RS02255 at 489.442 kb on + strand at 489.443 kb on - strand, within HGI48_RS02255 at 489.443 kb on - strand, within HGI48_RS02255 at 489.515 kb on + strand, within HGI48_RS02255 at 489.515 kb on + strand, within HGI48_RS02255 at 489.516 kb on - strand, within HGI48_RS02255 at 489.516 kb on - strand, within HGI48_RS02255 at 489.516 kb on - strand at 489.713 kb on + strand, within HGI48_RS02255 at 489.714 kb on - strand, within HGI48_RS02255 at 489.714 kb on - strand, within HGI48_RS02255 at 489.714 kb on - strand, within HGI48_RS02255 at 489.773 kb on + strand, within HGI48_RS02255 at 489.773 kb on + strand, within HGI48_RS02255 at 489.773 kb on + strand, within HGI48_RS02255 at 489.774 kb on - strand at 489.774 kb on - strand, within HGI48_RS02255 at 489.774 kb on - strand at 489.774 kb on - strand, within HGI48_RS02255 at 489.774 kb on - strand at 489.774 kb on - strand, within HGI48_RS02255 at 489.774 kb on - strand, within HGI48_RS02255 at 489.774 kb on - strand, within HGI48_RS02255 at 489.774 kb on - strand at 489.786 kb on + strand, within HGI48_RS02255 at 489.786 kb on + strand, within HGI48_RS02255 at 489.886 kb on + strand at 489.886 kb on + strand at 489.886 kb on + strand at 489.886 kb on + strand at 489.886 kb on + strand at 489.886 kb on + strand at 489.886 kb on + strand at 489.886 kb on + strand at 489.886 kb on + strand at 489.886 kb on + strand at 489.886 kb on + strand at 489.886 kb on + strand at 489.887 kb on - strand at 489.887 kb on - strand at 489.887 kb on - strand at 489.887 kb on - strand at 489.887 kb on - strand at 489.887 kb on - strand at 489.887 kb on - strand at 489.887 kb on - strand at 489.887 kb on - strand at 489.909 kb on + strand at 489.909 kb on + strand at 489.910 kb on - strand at 489.910 kb on - strand at 489.910 kb on - strand at 489.989 kb on + strand at 489.989 kb on + strand at 489.989 kb on + strand at 489.989 kb on + strand at 489.989 kb on + strand at 489.989 kb on + strand at 489.990 kb on - strand at 489.997 kb on + strand at 489.997 kb on + strand at 489.997 kb on + strand at 489.997 kb on + strand at 489.998 kb on - strand at 489.998 kb on - strand at 489.998 kb on - strand at 490.046 kb on + strand at 490.047 kb on - strand at 490.047 kb on - strand at 490.047 kb on - strand at 490.050 kb on + strand at 490.051 kb on - strand at 490.051 kb on - strand at 490.051 kb on - strand at 490.051 kb on - strand at 490.090 kb on - strand at 490.147 kb on + strand at 490.147 kb on + strand at 490.148 kb on - strand at 490.148 kb on - strand at 490.148 kb on - strand at 490.148 kb on - strand at 490.148 kb on - strand at 490.233 kb on - strand at 490.240 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 488,430 + +0.1 488,432 + -2.5 488,466 + -1.7 488,466 + -0.3 488,469 + +0.1 488,469 + +0.6 488,469 + -0.5 488,475 + +3.4 488,475 + +0.8 488,475 + -0.3 488,475 + +0.8 488,475 + +0.2 488,475 + -1.5 488,476 - -1.3 488,476 - +0.3 488,476 - -0.8 488,476 - +0.1 488,476 - -0.6 488,481 + -1.0 488,482 - -0.3 488,503 + HGI48_RS02240 0.20 +0.7 488,503 + HGI48_RS02240 0.20 -0.4 488,504 - HGI48_RS02240 0.21 +0.3 488,504 - HGI48_RS02240 0.21 +0.1 488,517 - HGI48_RS02240 0.30 +0.7 488,541 + HGI48_RS02240 0.46 +2.1 488,553 + HGI48_RS02240 0.54 +0.5 488,557 - HGI48_RS02240 0.57 +0.1 488,557 - HGI48_RS02240 0.57 -0.3 488,557 - HGI48_RS02240 0.57 +0.2 488,617 + -0.5 488,618 - +0.3 488,618 - +0.4 488,637 + -0.8 488,637 + +1.3 488,638 - -0.3 488,644 - -0.1 488,734 + HGI48_RS02245 0.12 -2.1 488,813 + HGI48_RS02245 0.28 -0.5 488,813 + HGI48_RS02245 0.28 -0.5 488,975 + -0.5 488,992 + HGI48_RS02245 0.64 +0.4 488,992 + HGI48_RS02245 0.64 -0.7 488,993 - HGI48_RS02245 0.64 +0.3 489,092 + HGI48_RS02245 0.84 +0.6 489,093 - HGI48_RS02245 0.84 -0.9 489,201 + +0.3 489,201 + -0.2 489,202 - +0.4 489,202 - -0.4 489,202 - -1.3 489,209 - -0.7 489,262 + HGI48_RS02250 0.23 +0.3 489,262 + HGI48_RS02250 0.23 -0.5 489,263 - HGI48_RS02250 0.24 -1.5 489,263 - HGI48_RS02250 0.24 -2.6 489,265 + HGI48_RS02250 0.25 -0.6 489,265 + HGI48_RS02250 0.25 -0.2 489,265 + HGI48_RS02250 0.25 -0.4 489,266 - HGI48_RS02250 0.26 +1.7 489,272 + HGI48_RS02250 0.30 +0.3 489,273 - HGI48_RS02250 0.31 -0.3 489,273 - HGI48_RS02250 0.31 +0.4 489,322 + HGI48_RS02250 0.67 +1.5 489,322 + HGI48_RS02250 0.67 +2.6 489,323 - HGI48_RS02250 0.67 +0.3 489,358 - -0.1 489,358 - +0.1 489,358 - +0.5 489,358 - +0.1 489,365 + +0.6 489,366 - +0.3 489,440 + HGI48_RS02255 0.16 +0.6 489,440 + HGI48_RS02255 0.16 -0.2 489,440 + HGI48_RS02255 0.16 +0.4 489,441 - HGI48_RS02255 0.16 +0.7 489,441 - HGI48_RS02255 0.16 +1.4 489,442 + -0.5 489,443 - HGI48_RS02255 0.16 +0.9 489,443 - HGI48_RS02255 0.16 +1.6 489,515 + HGI48_RS02255 0.30 -0.4 489,515 + HGI48_RS02255 0.30 -0.4 489,516 - HGI48_RS02255 0.30 +0.4 489,516 - HGI48_RS02255 0.30 +1.3 489,516 - -0.5 489,713 + HGI48_RS02255 0.67 +0.0 489,714 - HGI48_RS02255 0.67 -0.9 489,714 - HGI48_RS02255 0.67 +0.5 489,714 - HGI48_RS02255 0.67 +0.5 489,773 + HGI48_RS02255 0.79 -0.5 489,773 + HGI48_RS02255 0.79 +1.4 489,773 + HGI48_RS02255 0.79 -1.5 489,774 - -0.5 489,774 - HGI48_RS02255 0.79 -1.3 489,774 - +0.5 489,774 - HGI48_RS02255 0.79 +1.1 489,774 - +0.5 489,774 - HGI48_RS02255 0.79 -2.1 489,774 - HGI48_RS02255 0.79 +0.5 489,774 - HGI48_RS02255 0.79 +0.6 489,774 - +1.1 489,786 + HGI48_RS02255 0.81 -1.5 489,786 + HGI48_RS02255 0.81 -0.5 489,886 + -0.6 489,886 + +0.5 489,886 + +1.4 489,886 + -1.8 489,886 + -0.9 489,886 + -0.5 489,886 + +0.1 489,886 + +0.7 489,886 + +0.1 489,886 + +1.4 489,886 + -0.9 489,886 + +0.6 489,887 - +0.2 489,887 - -1.3 489,887 - -2.3 489,887 - -1.2 489,887 - +0.6 489,887 - +0.7 489,887 - -1.5 489,887 - -1.3 489,887 - +1.9 489,909 + -1.3 489,909 + -0.8 489,910 - +0.3 489,910 - -1.5 489,910 - -0.2 489,989 + -0.5 489,989 + +0.0 489,989 + -1.9 489,989 + -0.9 489,989 + -0.3 489,989 + +0.8 489,990 - -1.3 489,997 + +2.5 489,997 + +0.3 489,997 + +2.5 489,997 + +0.8 489,998 - -0.8 489,998 - +0.3 489,998 - -0.5 490,046 + -2.4 490,047 - +0.7 490,047 - -0.1 490,047 - -1.3 490,050 + -1.1 490,051 - +1.1 490,051 - -0.9 490,051 - -2.3 490,051 - -0.1 490,090 - +0.7 490,147 + -0.9 490,147 + -0.8 490,148 - -1.9 490,148 - +0.1 490,148 - -0.8 490,148 - -1.3 490,148 - -0.2 490,233 - +1.1 490,240 + +0.5
Or see this region's nucleotide sequence