Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01915 and HGI48_RS01920 are separated by 162 nucleotides HGI48_RS01920 and HGI48_RS01925 are separated by 89 nucleotides HGI48_RS01925 and HGI48_RS01930 are separated by 282 nucleotides
HGI48_RS01915: HGI48_RS01915 - ornithine carbamoyltransferase, at 423,961 to 424,977
_RS01915
HGI48_RS01920: HGI48_RS01920 - ribonuclease E inhibitor RraB, at 425,140 to 425,568
_RS01920
HGI48_RS01925: HGI48_RS01925 - GNAT family N-acetyltransferase, at 425,658 to 426,161
_RS01925
HGI48_RS01930: HGI48_RS01930 - GNAT family N-acetyltransferase, at 426,444 to 426,926
_RS01930
Position (kb) 425
426
427 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 424.676 kb on + strand, within HGI48_RS01915 at 424.677 kb on - strand, within HGI48_RS01915 at 424.677 kb on - strand at 424.677 kb on - strand, within HGI48_RS01915 at 424.720 kb on - strand, within HGI48_RS01915 at 424.751 kb on + strand at 424.752 kb on - strand, within HGI48_RS01915 at 424.775 kb on + strand, within HGI48_RS01915 at 424.776 kb on - strand, within HGI48_RS01915 at 424.776 kb on - strand, within HGI48_RS01915 at 424.842 kb on + strand, within HGI48_RS01915 at 424.842 kb on + strand, within HGI48_RS01915 at 424.842 kb on + strand, within HGI48_RS01915 at 424.843 kb on - strand, within HGI48_RS01915 at 425.029 kb on + strand at 425.030 kb on - strand at 425.030 kb on - strand at 425.031 kb on + strand at 425.031 kb on + strand at 425.055 kb on + strand at 425.056 kb on - strand at 425.071 kb on + strand at 425.072 kb on - strand at 425.072 kb on - strand at 425.072 kb on - strand at 425.072 kb on - strand at 425.105 kb on - strand at 425.110 kb on + strand at 425.110 kb on + strand at 425.111 kb on - strand at 425.233 kb on + strand, within HGI48_RS01920 at 425.233 kb on + strand at 425.233 kb on + strand, within HGI48_RS01920 at 425.233 kb on + strand, within HGI48_RS01920 at 425.234 kb on - strand, within HGI48_RS01920 at 425.234 kb on - strand at 425.234 kb on - strand, within HGI48_RS01920 at 425.234 kb on - strand, within HGI48_RS01920 at 425.312 kb on - strand, within HGI48_RS01920 at 425.377 kb on - strand at 425.377 kb on - strand, within HGI48_RS01920 at 425.407 kb on - strand, within HGI48_RS01920 at 425.461 kb on + strand, within HGI48_RS01920 at 425.461 kb on + strand, within HGI48_RS01920 at 425.461 kb on + strand, within HGI48_RS01920 at 425.461 kb on + strand, within HGI48_RS01920 at 425.461 kb on + strand, within HGI48_RS01920 at 425.462 kb on - strand, within HGI48_RS01920 at 425.462 kb on - strand, within HGI48_RS01920 at 425.462 kb on - strand, within HGI48_RS01920 at 425.462 kb on - strand, within HGI48_RS01920 at 425.462 kb on - strand, within HGI48_RS01920 at 425.479 kb on + strand, within HGI48_RS01920 at 425.479 kb on + strand, within HGI48_RS01920 at 425.479 kb on + strand, within HGI48_RS01920 at 425.479 kb on + strand, within HGI48_RS01920 at 425.479 kb on + strand, within HGI48_RS01920 at 425.480 kb on - strand, within HGI48_RS01920 at 425.480 kb on - strand at 425.536 kb on + strand at 425.536 kb on + strand at 425.536 kb on + strand at 425.536 kb on + strand at 425.536 kb on + strand at 425.536 kb on + strand at 425.536 kb on + strand at 425.536 kb on + strand at 425.537 kb on - strand at 425.537 kb on - strand at 425.537 kb on - strand at 425.537 kb on - strand at 425.537 kb on - strand at 425.537 kb on - strand at 425.537 kb on - strand at 425.578 kb on + strand at 425.578 kb on + strand at 425.578 kb on + strand at 425.578 kb on + strand at 425.578 kb on + strand at 425.579 kb on - strand at 425.579 kb on - strand at 425.579 kb on - strand at 425.579 kb on - strand at 425.659 kb on + strand at 425.659 kb on + strand at 425.659 kb on + strand at 425.659 kb on + strand at 425.660 kb on - strand at 425.660 kb on - strand at 425.664 kb on + strand at 425.723 kb on + strand, within HGI48_RS01925 at 425.724 kb on - strand, within HGI48_RS01925 at 425.724 kb on - strand, within HGI48_RS01925 at 425.724 kb on - strand, within HGI48_RS01925 at 425.724 kb on - strand, within HGI48_RS01925 at 425.724 kb on - strand, within HGI48_RS01925 at 425.724 kb on - strand, within HGI48_RS01925 at 425.788 kb on + strand, within HGI48_RS01925 at 425.789 kb on - strand, within HGI48_RS01925 at 425.789 kb on - strand, within HGI48_RS01925 at 425.881 kb on + strand, within HGI48_RS01925 at 425.903 kb on + strand, within HGI48_RS01925 at 425.904 kb on - strand, within HGI48_RS01925 at 425.998 kb on + strand, within HGI48_RS01925 at 425.999 kb on - strand, within HGI48_RS01925 at 425.999 kb on - strand, within HGI48_RS01925 at 426.039 kb on - strand, within HGI48_RS01925 at 426.040 kb on + strand, within HGI48_RS01925 at 426.040 kb on + strand, within HGI48_RS01925 at 426.041 kb on - strand, within HGI48_RS01925 at 426.041 kb on - strand, within HGI48_RS01925 at 426.041 kb on - strand, within HGI48_RS01925 at 426.232 kb on + strand at 426.232 kb on + strand at 426.232 kb on + strand at 426.233 kb on - strand at 426.233 kb on - strand at 426.233 kb on - strand at 426.233 kb on - strand at 426.233 kb on - strand at 426.233 kb on - strand at 426.233 kb on - strand at 426.237 kb on - strand at 426.237 kb on - strand at 426.237 kb on - strand at 426.237 kb on - strand at 426.237 kb on - strand at 426.248 kb on + strand at 426.248 kb on + strand at 426.248 kb on + strand at 426.248 kb on + strand at 426.249 kb on - strand at 426.249 kb on - strand at 426.249 kb on - strand at 426.249 kb on - strand at 426.253 kb on - strand at 426.263 kb on - strand at 426.264 kb on + strand at 426.264 kb on + strand at 426.264 kb on + strand at 426.264 kb on + strand at 426.264 kb on + strand at 426.264 kb on + strand at 426.264 kb on + strand at 426.265 kb on - strand at 426.265 kb on - strand at 426.265 kb on - strand at 426.265 kb on - strand at 426.269 kb on - strand at 426.269 kb on - strand at 426.295 kb on + strand at 426.295 kb on + strand at 426.296 kb on - strand at 426.296 kb on - strand at 426.297 kb on + strand at 426.297 kb on + strand at 426.297 kb on + strand at 426.306 kb on + strand at 426.307 kb on - strand at 426.358 kb on + strand at 426.359 kb on - strand at 426.359 kb on - strand at 426.401 kb on + strand at 426.401 kb on + strand at 426.402 kb on - strand at 426.443 kb on + strand at 426.526 kb on + strand, within HGI48_RS01930 at 426.526 kb on + strand, within HGI48_RS01930 at 426.548 kb on + strand, within HGI48_RS01930 at 426.549 kb on - strand, within HGI48_RS01930 at 426.549 kb on - strand, within HGI48_RS01930 at 426.549 kb on - strand, within HGI48_RS01930 at 426.593 kb on - strand, within HGI48_RS01930 at 426.840 kb on + strand, within HGI48_RS01930 at 426.883 kb on - strand at 426.924 kb on + strand at 426.925 kb on - strand at 426.942 kb on - strand at 426.942 kb on - strand at 426.960 kb on + strand at 426.961 kb on - strand at 426.972 kb on + strand at 426.973 kb on - strand at 427.060 kb on + strand at 427.061 kb on - strand at 427.061 kb on - strand at 427.061 kb on - strand at 427.078 kb on + strand at 427.079 kb on - strand at 427.095 kb on - strand at 427.109 kb on - strand at 427.110 kb on + strand at 427.111 kb on - strand at 427.143 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 424,676 + HGI48_RS01915 0.70 -2.9 424,677 - HGI48_RS01915 0.70 -0.5 424,677 - -0.5 424,677 - HGI48_RS01915 0.70 -3.5 424,720 - HGI48_RS01915 0.75 +0.1 424,751 + +1.7 424,752 - HGI48_RS01915 0.78 +2.1 424,775 + HGI48_RS01915 0.80 -2.6 424,776 - HGI48_RS01915 0.80 -1.9 424,776 - HGI48_RS01915 0.80 +0.7 424,842 + HGI48_RS01915 0.87 -1.1 424,842 + HGI48_RS01915 0.87 -0.3 424,842 + HGI48_RS01915 0.87 -1.5 424,843 - HGI48_RS01915 0.87 -1.9 425,029 + +0.3 425,030 - +0.9 425,030 - +1.7 425,031 + -0.9 425,031 + +0.4 425,055 + -0.6 425,056 - +0.8 425,071 + -0.5 425,072 - -0.2 425,072 - +0.1 425,072 - +0.1 425,072 - +0.9 425,105 - +0.5 425,110 + -1.3 425,110 + +0.1 425,111 - +1.7 425,233 + HGI48_RS01920 0.22 +1.7 425,233 + +2.5 425,233 + HGI48_RS01920 0.22 -0.6 425,233 + HGI48_RS01920 0.22 +0.1 425,234 - HGI48_RS01920 0.22 +0.2 425,234 - +1.8 425,234 - HGI48_RS01920 0.22 -0.7 425,234 - HGI48_RS01920 0.22 +0.7 425,312 - HGI48_RS01920 0.40 +0.1 425,377 - +3.1 425,377 - HGI48_RS01920 0.55 -0.5 425,407 - HGI48_RS01920 0.62 -2.4 425,461 + HGI48_RS01920 0.75 -0.2 425,461 + HGI48_RS01920 0.75 +1.1 425,461 + HGI48_RS01920 0.75 -2.1 425,461 + HGI48_RS01920 0.75 +0.3 425,461 + HGI48_RS01920 0.75 +0.5 425,462 - HGI48_RS01920 0.75 +0.9 425,462 - HGI48_RS01920 0.75 +1.1 425,462 - HGI48_RS01920 0.75 +0.6 425,462 - HGI48_RS01920 0.75 -0.7 425,462 - HGI48_RS01920 0.75 +0.2 425,479 + HGI48_RS01920 0.79 -0.2 425,479 + HGI48_RS01920 0.79 +0.7 425,479 + HGI48_RS01920 0.79 +0.2 425,479 + HGI48_RS01920 0.79 -1.7 425,479 + HGI48_RS01920 0.79 -0.9 425,480 - HGI48_RS01920 0.79 -0.8 425,480 - -0.5 425,536 + -0.9 425,536 + +0.4 425,536 + +1.1 425,536 + -0.6 425,536 + +0.1 425,536 + -0.5 425,536 + +1.1 425,536 + -1.6 425,537 - -0.2 425,537 - +0.5 425,537 - -1.7 425,537 - -0.3 425,537 - +1.5 425,537 - +0.9 425,537 - +0.5 425,578 + -1.7 425,578 + +0.1 425,578 + -0.7 425,578 + -1.6 425,578 + +1.1 425,579 - -0.1 425,579 - +0.7 425,579 - +2.3 425,579 - +0.5 425,659 + -1.0 425,659 + -0.5 425,659 + -2.8 425,659 + -1.2 425,660 - +1.4 425,660 - +1.9 425,664 + -0.1 425,723 + HGI48_RS01925 0.13 -1.4 425,724 - HGI48_RS01925 0.13 +0.3 425,724 - HGI48_RS01925 0.13 -0.7 425,724 - HGI48_RS01925 0.13 -0.7 425,724 - HGI48_RS01925 0.13 -1.5 425,724 - HGI48_RS01925 0.13 -0.3 425,724 - HGI48_RS01925 0.13 -0.5 425,788 + HGI48_RS01925 0.26 +1.1 425,789 - HGI48_RS01925 0.26 +0.6 425,789 - HGI48_RS01925 0.26 +2.1 425,881 + HGI48_RS01925 0.44 -1.1 425,903 + HGI48_RS01925 0.49 -0.8 425,904 - HGI48_RS01925 0.49 +0.7 425,998 + HGI48_RS01925 0.67 +0.9 425,999 - HGI48_RS01925 0.68 -0.9 425,999 - HGI48_RS01925 0.68 -0.9 426,039 - HGI48_RS01925 0.76 -1.7 426,040 + HGI48_RS01925 0.76 +1.1 426,040 + HGI48_RS01925 0.76 +0.3 426,041 - HGI48_RS01925 0.76 -0.5 426,041 - HGI48_RS01925 0.76 +0.7 426,041 - HGI48_RS01925 0.76 -2.2 426,232 + +0.2 426,232 + -0.9 426,232 + -0.5 426,233 - -0.7 426,233 - -0.7 426,233 - +2.8 426,233 - -0.4 426,233 - +0.1 426,233 - +1.3 426,233 - -1.9 426,237 - -0.3 426,237 - -0.9 426,237 - +1.0 426,237 - -2.8 426,237 - -1.9 426,248 + -0.5 426,248 + -1.9 426,248 + +1.1 426,248 + -0.1 426,249 - -0.4 426,249 - +0.1 426,249 - +1.7 426,249 - -1.7 426,253 - -2.0 426,263 - -0.1 426,264 + -0.5 426,264 + -0.1 426,264 + +0.1 426,264 + +0.5 426,264 + +0.1 426,264 + +1.1 426,264 + -0.5 426,265 - +1.6 426,265 - -3.1 426,265 - +0.3 426,265 - +0.1 426,269 - +0.7 426,269 - +0.5 426,295 + -0.7 426,295 + -0.2 426,296 - -0.8 426,296 - -0.4 426,297 + -0.8 426,297 + +0.9 426,297 + +1.5 426,306 + -0.5 426,307 - -2.5 426,358 + -1.5 426,359 - +0.8 426,359 - -0.4 426,401 + -0.5 426,401 + +1.5 426,402 - -1.5 426,443 + +0.7 426,526 + HGI48_RS01930 0.17 +0.1 426,526 + HGI48_RS01930 0.17 +0.7 426,548 + HGI48_RS01930 0.22 -0.9 426,549 - HGI48_RS01930 0.22 -1.5 426,549 - HGI48_RS01930 0.22 -0.3 426,549 - HGI48_RS01930 0.22 +0.9 426,593 - HGI48_RS01930 0.31 -1.2 426,840 + HGI48_RS01930 0.82 -1.0 426,883 - +0.8 426,924 + -2.7 426,925 - +0.1 426,942 - -0.7 426,942 - -0.2 426,960 + +1.5 426,961 - -1.5 426,972 + +0.5 426,973 - +0.1 427,060 + +0.1 427,061 - -0.1 427,061 - -1.2 427,061 - -0.8 427,078 + -1.4 427,079 - -0.7 427,095 - -1.2 427,109 - +0.1 427,110 + +0.1 427,111 - -2.2 427,143 + -0.1
Or see this region's nucleotide sequence