Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01150 and HGI48_RS01155 are separated by 1 nucleotides HGI48_RS01155 and HGI48_RS01160 overlap by 4 nucleotides HGI48_RS01160 and HGI48_RS01165 are separated by 1 nucleotides HGI48_RS01165 and HGI48_RS01170 overlap by 1 nucleotides
HGI48_RS01150: HGI48_RS01150 - thiazole synthase, at 259,315 to 260,094
_RS01150
HGI48_RS01155: HGI48_RS01155 - sulfur carrier protein ThiS, at 260,096 to 260,296
_RS01155
HGI48_RS01160: HGI48_RS01160 - HesA/MoeB/ThiF family protein, at 260,293 to 261,045
_RS01160
HGI48_RS01165: HGI48_RS01165 - thiamine phosphate synthase, at 261,047 to 261,682
_RS01165
HGI48_RS01170: HGI48_RS01170 - phosphomethylpyrimidine synthase ThiC, at 261,682 to 263,553
_RS01170
Position (kb) 261
262 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 260.067 kb on + strand at 260.067 kb on + strand at 260.338 kb on + strand at 260.338 kb on + strand at 260.476 kb on + strand, within HGI48_RS01160 at 260.535 kb on - strand, within HGI48_RS01160 at 260.549 kb on + strand, within HGI48_RS01160 at 260.563 kb on + strand, within HGI48_RS01160 at 260.854 kb on + strand, within HGI48_RS01160 at 260.854 kb on + strand at 260.854 kb on + strand at 260.906 kb on + strand, within HGI48_RS01160 at 260.906 kb on + strand, within HGI48_RS01160 at 260.907 kb on - strand, within HGI48_RS01160 at 260.907 kb on - strand, within HGI48_RS01160 at 261.025 kb on - strand at 261.025 kb on - strand at 261.025 kb on - strand at 261.048 kb on + strand at 261.078 kb on - strand at 261.078 kb on - strand at 261.295 kb on + strand, within HGI48_RS01165 at 261.295 kb on + strand at 261.310 kb on + strand at 261.310 kb on + strand, within HGI48_RS01165 at 261.311 kb on - strand, within HGI48_RS01165 at 261.311 kb on - strand, within HGI48_RS01165 at 261.312 kb on + strand at 261.312 kb on + strand, within HGI48_RS01165 at 261.312 kb on + strand, within HGI48_RS01165 at 261.312 kb on + strand, within HGI48_RS01165 at 261.313 kb on - strand, within HGI48_RS01165 at 261.313 kb on - strand, within HGI48_RS01165 at 261.313 kb on - strand, within HGI48_RS01165 at 261.313 kb on - strand, within HGI48_RS01165 at 261.313 kb on - strand, within HGI48_RS01165 at 261.313 kb on - strand, within HGI48_RS01165 at 261.313 kb on - strand, within HGI48_RS01165 at 261.313 kb on - strand at 261.313 kb on - strand, within HGI48_RS01165 at 261.377 kb on + strand, within HGI48_RS01165 at 261.437 kb on + strand, within HGI48_RS01165 at 261.437 kb on + strand, within HGI48_RS01165 at 261.437 kb on + strand, within HGI48_RS01165 at 261.438 kb on - strand, within HGI48_RS01165 at 261.438 kb on - strand, within HGI48_RS01165 at 261.445 kb on - strand, within HGI48_RS01165 at 261.474 kb on + strand, within HGI48_RS01165 at 261.475 kb on - strand, within HGI48_RS01165 at 261.572 kb on + strand, within HGI48_RS01165 at 261.572 kb on + strand, within HGI48_RS01165 at 261.572 kb on + strand, within HGI48_RS01165 at 261.573 kb on - strand, within HGI48_RS01165 at 261.573 kb on - strand, within HGI48_RS01165 at 261.584 kb on - strand, within HGI48_RS01165 at 261.719 kb on + strand at 261.720 kb on - strand at 261.720 kb on - strand at 261.721 kb on + strand at 261.721 kb on + strand at 261.721 kb on + strand at 261.721 kb on + strand at 261.721 kb on + strand at 261.722 kb on - strand at 261.722 kb on - strand at 261.722 kb on - strand at 261.722 kb on - strand at 261.722 kb on - strand at 261.722 kb on - strand at 261.722 kb on - strand at 261.722 kb on - strand at 261.809 kb on + strand at 261.828 kb on + strand at 261.829 kb on - strand at 261.878 kb on - strand, within HGI48_RS01170 at 262.051 kb on + strand, within HGI48_RS01170 at 262.059 kb on - strand, within HGI48_RS01170 at 262.124 kb on + strand, within HGI48_RS01170 at 262.124 kb on + strand, within HGI48_RS01170 at 262.125 kb on - strand, within HGI48_RS01170 at 262.125 kb on - strand, within HGI48_RS01170 at 262.125 kb on - strand at 262.125 kb on - strand, within HGI48_RS01170 at 262.222 kb on + strand, within HGI48_RS01170 at 262.223 kb on - strand, within HGI48_RS01170 at 262.223 kb on - strand, within HGI48_RS01170 at 262.223 kb on - strand, within HGI48_RS01170 at 262.229 kb on + strand, within HGI48_RS01170 at 262.294 kb on + strand, within HGI48_RS01170 at 262.294 kb on + strand at 262.294 kb on + strand, within HGI48_RS01170 at 262.294 kb on + strand, within HGI48_RS01170 at 262.295 kb on - strand, within HGI48_RS01170 at 262.295 kb on - strand, within HGI48_RS01170 at 262.332 kb on - strand, within HGI48_RS01170 at 262.332 kb on - strand, within HGI48_RS01170 at 262.420 kb on + strand, within HGI48_RS01170 at 262.491 kb on - strand, within HGI48_RS01170 at 262.491 kb on - strand, within HGI48_RS01170 at 262.527 kb on + strand, within HGI48_RS01170 at 262.527 kb on + strand, within HGI48_RS01170 at 262.528 kb on - strand, within HGI48_RS01170 at 262.623 kb on - strand, within HGI48_RS01170
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 260,067 + +1.1 260,067 + -1.9 260,338 + -1.9 260,338 + -1.8 260,476 + HGI48_RS01160 0.24 +0.1 260,535 - HGI48_RS01160 0.32 -0.5 260,549 + HGI48_RS01160 0.34 +0.7 260,563 + HGI48_RS01160 0.36 -2.5 260,854 + HGI48_RS01160 0.75 +0.2 260,854 + +0.1 260,854 + -0.5 260,906 + HGI48_RS01160 0.81 -1.7 260,906 + HGI48_RS01160 0.81 -1.5 260,907 - HGI48_RS01160 0.82 -0.3 260,907 - HGI48_RS01160 0.82 -0.1 261,025 - -1.0 261,025 - +0.7 261,025 - -1.2 261,048 + -0.5 261,078 - -0.7 261,078 - -2.3 261,295 + HGI48_RS01165 0.39 +0.7 261,295 + +1.1 261,310 + -0.5 261,310 + HGI48_RS01165 0.41 -0.7 261,311 - HGI48_RS01165 0.42 -0.6 261,311 - HGI48_RS01165 0.42 -1.7 261,312 + +0.1 261,312 + HGI48_RS01165 0.42 +2.4 261,312 + HGI48_RS01165 0.42 -0.5 261,312 + HGI48_RS01165 0.42 +0.1 261,313 - HGI48_RS01165 0.42 +1.1 261,313 - HGI48_RS01165 0.42 -0.6 261,313 - HGI48_RS01165 0.42 +0.5 261,313 - HGI48_RS01165 0.42 -0.4 261,313 - HGI48_RS01165 0.42 +1.6 261,313 - HGI48_RS01165 0.42 -0.3 261,313 - HGI48_RS01165 0.42 -1.7 261,313 - -0.5 261,313 - HGI48_RS01165 0.42 -1.7 261,377 + HGI48_RS01165 0.52 -1.7 261,437 + HGI48_RS01165 0.61 +0.1 261,437 + HGI48_RS01165 0.61 +0.1 261,437 + HGI48_RS01165 0.61 -1.0 261,438 - HGI48_RS01165 0.61 +1.9 261,438 - HGI48_RS01165 0.61 -1.2 261,445 - HGI48_RS01165 0.63 -0.9 261,474 + HGI48_RS01165 0.67 +1.4 261,475 - HGI48_RS01165 0.67 +0.2 261,572 + HGI48_RS01165 0.83 +1.5 261,572 + HGI48_RS01165 0.83 -0.2 261,572 + HGI48_RS01165 0.83 -0.8 261,573 - HGI48_RS01165 0.83 -1.8 261,573 - HGI48_RS01165 0.83 -2.1 261,584 - HGI48_RS01165 0.84 -0.3 261,719 + -0.9 261,720 - +0.1 261,720 - +0.5 261,721 + -1.2 261,721 + -0.1 261,721 + -2.6 261,721 + +0.0 261,721 + -0.3 261,722 - -2.5 261,722 - -1.9 261,722 - -0.1 261,722 - -3.0 261,722 - -1.5 261,722 - -0.5 261,722 - -1.7 261,722 - -0.2 261,809 + -0.6 261,828 + -0.9 261,829 - -1.2 261,878 - HGI48_RS01170 0.10 -2.2 262,051 + HGI48_RS01170 0.20 -1.6 262,059 - HGI48_RS01170 0.20 -1.5 262,124 + HGI48_RS01170 0.24 -1.6 262,124 + HGI48_RS01170 0.24 -1.2 262,125 - HGI48_RS01170 0.24 -0.2 262,125 - HGI48_RS01170 0.24 -0.6 262,125 - +2.4 262,125 - HGI48_RS01170 0.24 +2.3 262,222 + HGI48_RS01170 0.29 +0.6 262,223 - HGI48_RS01170 0.29 -1.7 262,223 - HGI48_RS01170 0.29 -1.5 262,223 - HGI48_RS01170 0.29 -1.2 262,229 + HGI48_RS01170 0.29 +0.6 262,294 + HGI48_RS01170 0.33 -1.2 262,294 + -0.5 262,294 + HGI48_RS01170 0.33 +0.6 262,294 + HGI48_RS01170 0.33 +1.1 262,295 - HGI48_RS01170 0.33 +0.6 262,295 - HGI48_RS01170 0.33 -0.3 262,332 - HGI48_RS01170 0.35 -1.3 262,332 - HGI48_RS01170 0.35 -0.9 262,420 + HGI48_RS01170 0.39 -0.3 262,491 - HGI48_RS01170 0.43 +0.0 262,491 - HGI48_RS01170 0.43 +1.5 262,527 + HGI48_RS01170 0.45 +0.2 262,527 + HGI48_RS01170 0.45 -1.9 262,528 - HGI48_RS01170 0.45 -0.6 262,623 - HGI48_RS01170 0.50 +0.9
Or see this region's nucleotide sequence