Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS10400 and HGI48_RS10405 are separated by 109 nucleotides HGI48_RS10405 and HGI48_RS10410 are separated by 53 nucleotides HGI48_RS10410 and HGI48_RS10415 are separated by 243 nucleotides HGI48_RS10415 and HGI48_RS10420 are separated by 68 nucleotides
HGI48_RS10400: HGI48_RS10400 - murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF, at 2,245,262 to 2,246,266
_RS10400
HGI48_RS10405: HGI48_RS10405 - YciU family protein, at 2,246,376 to 2,246,699
_RS10405
HGI48_RS10410: HGI48_RS10410 - cardiolipin synthase, at 2,246,753 to 2,248,213
_RS10410
HGI48_RS10415: HGI48_RS10415 - YciY family protein, at 2,248,457 to 2,248,633
_RS10415
HGI48_RS10420: HGI48_RS10420 - Tar ligand binding domain-containing protein, at 2,248,702 to 2,250,402
_RS10420
Position (kb)
2246
2247
2248
2249 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2245.774 kb on + strand, within HGI48_RS10400 at 2245.970 kb on - strand, within HGI48_RS10400 at 2245.973 kb on + strand, within HGI48_RS10400 at 2245.973 kb on + strand at 2245.973 kb on + strand, within HGI48_RS10400 at 2245.973 kb on + strand, within HGI48_RS10400 at 2245.974 kb on - strand at 2246.015 kb on + strand, within HGI48_RS10400 at 2246.036 kb on + strand, within HGI48_RS10400 at 2246.036 kb on + strand, within HGI48_RS10400 at 2246.037 kb on - strand, within HGI48_RS10400 at 2246.037 kb on - strand, within HGI48_RS10400 at 2246.154 kb on + strand, within HGI48_RS10400 at 2246.154 kb on + strand, within HGI48_RS10400 at 2246.155 kb on - strand, within HGI48_RS10400 at 2246.264 kb on + strand at 2246.265 kb on - strand at 2246.285 kb on + strand at 2246.286 kb on - strand at 2246.286 kb on - strand at 2246.300 kb on - strand at 2246.302 kb on + strand at 2246.650 kb on + strand, within HGI48_RS10405 at 2246.651 kb on - strand, within HGI48_RS10405 at 2246.698 kb on + strand at 2246.699 kb on + strand at 2246.699 kb on + strand at 2246.700 kb on - strand at 2246.700 kb on - strand at 2246.700 kb on - strand at 2246.700 kb on - strand at 2246.732 kb on - strand at 2246.754 kb on + strand at 2246.754 kb on + strand at 2246.755 kb on - strand at 2246.856 kb on + strand at 2246.856 kb on + strand at 2246.856 kb on + strand at 2246.856 kb on + strand at 2246.856 kb on + strand at 2246.856 kb on + strand at 2246.856 kb on + strand at 2246.856 kb on + strand at 2246.856 kb on + strand at 2246.856 kb on + strand at 2246.856 kb on + strand at 2246.856 kb on + strand at 2246.856 kb on + strand at 2246.857 kb on - strand at 2246.857 kb on - strand at 2246.857 kb on - strand at 2246.857 kb on - strand at 2246.857 kb on - strand at 2246.857 kb on - strand at 2246.857 kb on - strand at 2246.857 kb on - strand at 2246.857 kb on - strand at 2246.857 kb on - strand at 2246.857 kb on - strand at 2246.857 kb on - strand at 2246.857 kb on - strand at 2246.944 kb on + strand at 2246.998 kb on + strand, within HGI48_RS10410 at 2246.999 kb on - strand at 2246.999 kb on - strand, within HGI48_RS10410 at 2247.028 kb on - strand, within HGI48_RS10410 at 2247.028 kb on - strand, within HGI48_RS10410 at 2247.050 kb on + strand, within HGI48_RS10410 at 2247.051 kb on - strand, within HGI48_RS10410 at 2247.051 kb on - strand, within HGI48_RS10410 at 2247.149 kb on - strand, within HGI48_RS10410 at 2247.186 kb on + strand, within HGI48_RS10410 at 2247.187 kb on - strand, within HGI48_RS10410 at 2247.223 kb on - strand, within HGI48_RS10410 at 2247.253 kb on + strand, within HGI48_RS10410 at 2247.254 kb on - strand, within HGI48_RS10410 at 2247.283 kb on + strand, within HGI48_RS10410 at 2247.295 kb on + strand, within HGI48_RS10410 at 2247.295 kb on + strand, within HGI48_RS10410 at 2247.326 kb on - strand, within HGI48_RS10410 at 2247.326 kb on - strand at 2247.387 kb on + strand, within HGI48_RS10410 at 2247.387 kb on + strand, within HGI48_RS10410 at 2247.387 kb on + strand, within HGI48_RS10410 at 2247.388 kb on - strand, within HGI48_RS10410 at 2247.388 kb on - strand, within HGI48_RS10410 at 2247.388 kb on - strand at 2247.388 kb on - strand, within HGI48_RS10410 at 2247.388 kb on - strand at 2247.388 kb on - strand at 2247.388 kb on - strand, within HGI48_RS10410 at 2247.474 kb on + strand, within HGI48_RS10410 at 2247.475 kb on - strand, within HGI48_RS10410 at 2247.490 kb on + strand, within HGI48_RS10410 at 2247.491 kb on - strand at 2247.491 kb on - strand, within HGI48_RS10410 at 2247.491 kb on - strand, within HGI48_RS10410 at 2247.491 kb on - strand at 2247.507 kb on + strand, within HGI48_RS10410 at 2247.508 kb on - strand, within HGI48_RS10410 at 2247.514 kb on + strand, within HGI48_RS10410 at 2247.532 kb on + strand, within HGI48_RS10410 at 2247.538 kb on + strand, within HGI48_RS10410 at 2247.538 kb on + strand, within HGI48_RS10410 at 2247.539 kb on - strand, within HGI48_RS10410 at 2247.593 kb on + strand, within HGI48_RS10410 at 2247.640 kb on - strand, within HGI48_RS10410 at 2247.763 kb on + strand, within HGI48_RS10410 at 2247.765 kb on + strand, within HGI48_RS10410 at 2247.765 kb on + strand, within HGI48_RS10410 at 2247.765 kb on + strand, within HGI48_RS10410 at 2247.765 kb on + strand, within HGI48_RS10410 at 2247.765 kb on + strand, within HGI48_RS10410 at 2247.765 kb on + strand, within HGI48_RS10410 at 2247.766 kb on - strand, within HGI48_RS10410 at 2247.766 kb on - strand, within HGI48_RS10410 at 2247.766 kb on - strand, within HGI48_RS10410 at 2247.766 kb on - strand, within HGI48_RS10410 at 2247.766 kb on - strand, within HGI48_RS10410 at 2247.766 kb on - strand, within HGI48_RS10410 at 2247.766 kb on - strand, within HGI48_RS10410 at 2247.766 kb on - strand, within HGI48_RS10410 at 2247.766 kb on - strand, within HGI48_RS10410 at 2247.766 kb on - strand, within HGI48_RS10410 at 2247.766 kb on - strand, within HGI48_RS10410 at 2247.818 kb on - strand, within HGI48_RS10410 at 2247.818 kb on - strand, within HGI48_RS10410 at 2248.044 kb on - strand, within HGI48_RS10410 at 2248.050 kb on + strand, within HGI48_RS10410 at 2248.051 kb on - strand, within HGI48_RS10410 at 2248.051 kb on - strand, within HGI48_RS10410 at 2248.051 kb on - strand, within HGI48_RS10410 at 2248.051 kb on - strand, within HGI48_RS10410 at 2248.078 kb on + strand at 2248.079 kb on - strand at 2248.080 kb on + strand at 2248.081 kb on - strand at 2248.081 kb on - strand at 2248.081 kb on - strand at 2248.081 kb on - strand at 2248.081 kb on - strand at 2248.135 kb on + strand at 2248.135 kb on + strand at 2248.135 kb on + strand at 2248.144 kb on + strand at 2248.144 kb on + strand at 2248.144 kb on + strand at 2248.144 kb on + strand at 2248.145 kb on - strand at 2248.145 kb on - strand at 2248.145 kb on - strand at 2248.167 kb on + strand at 2248.168 kb on - strand at 2248.193 kb on + strand at 2248.193 kb on + strand at 2248.198 kb on + strand at 2248.200 kb on + strand at 2248.201 kb on - strand at 2248.201 kb on - strand at 2248.201 kb on - strand at 2248.201 kb on - strand at 2248.201 kb on - strand at 2248.213 kb on + strand at 2248.216 kb on + strand at 2248.217 kb on - strand at 2248.305 kb on + strand at 2248.305 kb on + strand at 2248.306 kb on - strand at 2248.307 kb on + strand at 2248.307 kb on + strand at 2248.307 kb on + strand at 2248.307 kb on + strand at 2248.308 kb on - strand at 2248.308 kb on - strand at 2248.315 kb on - strand at 2248.315 kb on - strand at 2248.379 kb on - strand at 2248.379 kb on - strand at 2248.380 kb on + strand at 2248.380 kb on + strand at 2248.380 kb on + strand at 2248.381 kb on - strand at 2248.381 kb on - strand at 2248.402 kb on + strand at 2248.402 kb on + strand at 2248.575 kb on - strand, within HGI48_RS10415 at 2248.610 kb on + strand, within HGI48_RS10415 at 2248.610 kb on + strand, within HGI48_RS10415 at 2248.611 kb on - strand, within HGI48_RS10415 at 2248.642 kb on + strand at 2248.642 kb on + strand at 2248.642 kb on + strand at 2248.703 kb on + strand at 2248.703 kb on + strand at 2248.704 kb on - strand at 2248.704 kb on - strand at 2248.704 kb on - strand at 2248.704 kb on - strand at 2248.737 kb on - strand at 2248.737 kb on - strand at 2248.848 kb on + strand at 2248.947 kb on + strand, within HGI48_RS10420 at 2248.947 kb on + strand, within HGI48_RS10420 at 2248.971 kb on + strand, within HGI48_RS10420 at 2248.972 kb on - strand, within HGI48_RS10420 at 2248.972 kb on - strand, within HGI48_RS10420 at 2248.972 kb on - strand, within HGI48_RS10420 at 2249.029 kb on + strand at 2249.030 kb on - strand, within HGI48_RS10420 at 2249.030 kb on - strand, within HGI48_RS10420
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 2,245,774 + HGI48_RS10400 0.51 +0.7 2,245,970 - HGI48_RS10400 0.70 -1.2 2,245,973 + HGI48_RS10400 0.71 -0.6 2,245,973 + -0.2 2,245,973 + HGI48_RS10400 0.71 -1.0 2,245,973 + HGI48_RS10400 0.71 +0.4 2,245,974 - +0.4 2,246,015 + HGI48_RS10400 0.75 +0.0 2,246,036 + HGI48_RS10400 0.77 +0.4 2,246,036 + HGI48_RS10400 0.77 -0.3 2,246,037 - HGI48_RS10400 0.77 +0.0 2,246,037 - HGI48_RS10400 0.77 -0.6 2,246,154 + HGI48_RS10400 0.89 -0.2 2,246,154 + HGI48_RS10400 0.89 +0.8 2,246,155 - HGI48_RS10400 0.89 -0.3 2,246,264 + +0.4 2,246,265 - -0.6 2,246,285 + -2.2 2,246,286 - +0.4 2,246,286 - -1.2 2,246,300 - -3.9 2,246,302 + -1.7 2,246,650 + HGI48_RS10405 0.85 -0.6 2,246,651 - HGI48_RS10405 0.85 +1.0 2,246,698 + -1.2 2,246,699 + -0.4 2,246,699 + -1.4 2,246,700 - -0.9 2,246,700 - -1.6 2,246,700 - +0.4 2,246,700 - +0.0 2,246,732 - +1.2 2,246,754 + +1.4 2,246,754 + +0.4 2,246,755 - +0.4 2,246,856 + +0.7 2,246,856 + -1.7 2,246,856 + -0.6 2,246,856 + -0.6 2,246,856 + -2.2 2,246,856 + +0.0 2,246,856 + -0.2 2,246,856 + -0.6 2,246,856 + -2.3 2,246,856 + -2.6 2,246,856 + -2.6 2,246,856 + -2.4 2,246,856 + -0.6 2,246,857 - +0.0 2,246,857 - -1.0 2,246,857 - -1.0 2,246,857 - -0.6 2,246,857 - -0.6 2,246,857 - +0.0 2,246,857 - -1.4 2,246,857 - +0.4 2,246,857 - -1.4 2,246,857 - -0.5 2,246,857 - -0.7 2,246,857 - -1.1 2,246,857 - -0.7 2,246,944 + +0.8 2,246,998 + HGI48_RS10410 0.17 -1.0 2,246,999 - +1.4 2,246,999 - HGI48_RS10410 0.17 +1.0 2,247,028 - HGI48_RS10410 0.19 -1.6 2,247,028 - HGI48_RS10410 0.19 -1.6 2,247,050 + HGI48_RS10410 0.20 +0.1 2,247,051 - HGI48_RS10410 0.20 -0.7 2,247,051 - HGI48_RS10410 0.20 -2.6 2,247,149 - HGI48_RS10410 0.27 -0.9 2,247,186 + HGI48_RS10410 0.30 -3.8 2,247,187 - HGI48_RS10410 0.30 +0.0 2,247,223 - HGI48_RS10410 0.32 -2.2 2,247,253 + HGI48_RS10410 0.34 -1.2 2,247,254 - HGI48_RS10410 0.34 -0.1 2,247,283 + HGI48_RS10410 0.36 -0.6 2,247,295 + HGI48_RS10410 0.37 -2.6 2,247,295 + HGI48_RS10410 0.37 -0.1 2,247,326 - HGI48_RS10410 0.39 -2.6 2,247,326 - -0.6 2,247,387 + HGI48_RS10410 0.43 -0.8 2,247,387 + HGI48_RS10410 0.43 -0.8 2,247,387 + HGI48_RS10410 0.43 -1.9 2,247,388 - HGI48_RS10410 0.43 -0.2 2,247,388 - HGI48_RS10410 0.43 -0.9 2,247,388 - -1.2 2,247,388 - HGI48_RS10410 0.43 -1.6 2,247,388 - +0.4 2,247,388 - -0.2 2,247,388 - HGI48_RS10410 0.43 -0.6 2,247,474 + HGI48_RS10410 0.49 -3.3 2,247,475 - HGI48_RS10410 0.49 -1.6 2,247,490 + HGI48_RS10410 0.50 +1.1 2,247,491 - +0.4 2,247,491 - HGI48_RS10410 0.51 -0.3 2,247,491 - HGI48_RS10410 0.51 -1.9 2,247,491 - -1.2 2,247,507 + HGI48_RS10410 0.52 -3.7 2,247,508 - HGI48_RS10410 0.52 +0.4 2,247,514 + HGI48_RS10410 0.52 -0.2 2,247,532 + HGI48_RS10410 0.53 -2.2 2,247,538 + HGI48_RS10410 0.54 -2.4 2,247,538 + HGI48_RS10410 0.54 -2.6 2,247,539 - HGI48_RS10410 0.54 +0.7 2,247,593 + HGI48_RS10410 0.57 -1.6 2,247,640 - HGI48_RS10410 0.61 -1.3 2,247,763 + HGI48_RS10410 0.69 -0.6 2,247,765 + HGI48_RS10410 0.69 -2.4 2,247,765 + HGI48_RS10410 0.69 +0.3 2,247,765 + HGI48_RS10410 0.69 +0.4 2,247,765 + HGI48_RS10410 0.69 +0.6 2,247,765 + HGI48_RS10410 0.69 -2.0 2,247,765 + HGI48_RS10410 0.69 -1.8 2,247,766 - HGI48_RS10410 0.69 -1.4 2,247,766 - HGI48_RS10410 0.69 -0.7 2,247,766 - HGI48_RS10410 0.69 -0.3 2,247,766 - HGI48_RS10410 0.69 -0.4 2,247,766 - HGI48_RS10410 0.69 -0.5 2,247,766 - HGI48_RS10410 0.69 -0.6 2,247,766 - HGI48_RS10410 0.69 +0.6 2,247,766 - HGI48_RS10410 0.69 -2.5 2,247,766 - HGI48_RS10410 0.69 +0.3 2,247,766 - HGI48_RS10410 0.69 -2.4 2,247,766 - HGI48_RS10410 0.69 -2.3 2,247,818 - HGI48_RS10410 0.73 -1.9 2,247,818 - HGI48_RS10410 0.73 +1.0 2,248,044 - HGI48_RS10410 0.88 +1.2 2,248,050 + HGI48_RS10410 0.89 -0.3 2,248,051 - HGI48_RS10410 0.89 +0.2 2,248,051 - HGI48_RS10410 0.89 -1.2 2,248,051 - HGI48_RS10410 0.89 +0.4 2,248,051 - HGI48_RS10410 0.89 -0.6 2,248,078 + -0.6 2,248,079 - +0.2 2,248,080 + -0.7 2,248,081 - -1.6 2,248,081 - -0.9 2,248,081 - -3.0 2,248,081 - -1.9 2,248,081 - -1.7 2,248,135 + +0.0 2,248,135 + -1.9 2,248,135 + -1.2 2,248,144 + +0.1 2,248,144 + -1.2 2,248,144 + -2.4 2,248,144 + -1.4 2,248,145 - -1.3 2,248,145 - -3.2 2,248,145 - -1.4 2,248,167 + -0.7 2,248,168 - +0.6 2,248,193 + -0.6 2,248,193 + -0.3 2,248,198 + -2.6 2,248,200 + -1.5 2,248,201 - -0.5 2,248,201 - +0.4 2,248,201 - -0.8 2,248,201 - -1.0 2,248,201 - -0.2 2,248,213 + -1.4 2,248,216 + +0.6 2,248,217 - -0.6 2,248,305 + -0.9 2,248,305 + +0.4 2,248,306 - +1.8 2,248,307 + -0.1 2,248,307 + -0.3 2,248,307 + -0.7 2,248,307 + +1.1 2,248,308 - +0.1 2,248,308 - +1.3 2,248,315 - +0.4 2,248,315 - -0.4 2,248,379 - +1.8 2,248,379 - -1.4 2,248,380 + -0.6 2,248,380 + -0.3 2,248,380 + +1.4 2,248,381 - -0.6 2,248,381 - -0.8 2,248,402 + -1.9 2,248,402 + -1.2 2,248,575 - HGI48_RS10415 0.67 +0.8 2,248,610 + HGI48_RS10415 0.86 -0.4 2,248,610 + HGI48_RS10415 0.86 -0.3 2,248,611 - HGI48_RS10415 0.87 -0.2 2,248,642 + -0.3 2,248,642 + -0.3 2,248,642 + -0.6 2,248,703 + +0.4 2,248,703 + +0.9 2,248,704 - -0.8 2,248,704 - -1.1 2,248,704 - -0.9 2,248,704 - -2.2 2,248,737 - -0.6 2,248,737 - +1.4 2,248,848 + -0.0 2,248,947 + HGI48_RS10420 0.14 -0.3 2,248,947 + HGI48_RS10420 0.14 -2.4 2,248,971 + HGI48_RS10420 0.16 +0.2 2,248,972 - HGI48_RS10420 0.16 -1.3 2,248,972 - HGI48_RS10420 0.16 -0.2 2,248,972 - HGI48_RS10420 0.16 +1.0 2,249,029 + +1.8 2,249,030 - HGI48_RS10420 0.19 -0.9 2,249,030 - HGI48_RS10420 0.19 -0.6
Or see this region's nucleotide sequence