Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS10300 and HGI48_RS10305 are separated by 266 nucleotides HGI48_RS10305 and HGI48_RS10310 are separated by 84 nucleotides HGI48_RS10310 and HGI48_RS10315 are separated by 5 nucleotides HGI48_RS10315 and HGI48_RS10320 are separated by 45 nucleotides
HGI48_RS10300: HGI48_RS10300 - signal peptide peptidase SppA, at 2,221,733 to 2,223,577
_RS10300
HGI48_RS10305: HGI48_RS10305 - NAD(P)H nitroreductase, at 2,223,844 to 2,224,395
_RS10305
HGI48_RS10310: HGI48_RS10310 - DNA topoisomerase III, at 2,224,480 to 2,226,405
_RS10310
HGI48_RS10315: HGI48_RS10315 - DUF1496 domain-containing protein, at 2,226,411 to 2,226,806
_RS10315
HGI48_RS10320: HGI48_RS10320 - exodeoxyribonuclease III, at 2,226,852 to 2,227,658
_RS10320
Position (kb)
2224
2225
2226
2227 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2223.546 kb on - strand at 2223.606 kb on + strand at 2223.695 kb on - strand at 2223.755 kb on - strand at 2223.774 kb on - strand at 2223.991 kb on - strand, within HGI48_RS10305 at 2224.143 kb on + strand, within HGI48_RS10305 at 2224.318 kb on + strand, within HGI48_RS10305 at 2224.318 kb on + strand, within HGI48_RS10305 at 2224.319 kb on - strand, within HGI48_RS10305 at 2224.338 kb on + strand, within HGI48_RS10305 at 2224.368 kb on + strand at 2224.388 kb on - strand at 2224.388 kb on - strand at 2224.388 kb on - strand at 2224.388 kb on - strand at 2224.394 kb on - strand at 2224.403 kb on - strand at 2224.567 kb on - strand at 2224.639 kb on + strand at 2224.640 kb on - strand at 2224.640 kb on - strand at 2224.809 kb on - strand, within HGI48_RS10310 at 2224.831 kb on + strand, within HGI48_RS10310 at 2225.017 kb on + strand, within HGI48_RS10310 at 2225.017 kb on + strand, within HGI48_RS10310 at 2225.018 kb on - strand at 2225.018 kb on - strand, within HGI48_RS10310 at 2225.018 kb on - strand, within HGI48_RS10310 at 2225.019 kb on + strand, within HGI48_RS10310 at 2225.019 kb on + strand, within HGI48_RS10310 at 2225.019 kb on + strand, within HGI48_RS10310 at 2225.020 kb on - strand, within HGI48_RS10310 at 2225.314 kb on + strand, within HGI48_RS10310 at 2225.315 kb on - strand, within HGI48_RS10310 at 2225.316 kb on + strand, within HGI48_RS10310 at 2225.317 kb on - strand, within HGI48_RS10310 at 2225.317 kb on - strand at 2225.317 kb on - strand, within HGI48_RS10310 at 2225.323 kb on - strand, within HGI48_RS10310 at 2225.437 kb on + strand, within HGI48_RS10310 at 2225.457 kb on + strand, within HGI48_RS10310 at 2225.458 kb on - strand, within HGI48_RS10310 at 2225.458 kb on - strand, within HGI48_RS10310 at 2225.686 kb on + strand, within HGI48_RS10310 at 2225.686 kb on + strand at 2225.707 kb on + strand, within HGI48_RS10310 at 2225.707 kb on + strand, within HGI48_RS10310 at 2225.708 kb on - strand, within HGI48_RS10310 at 2225.742 kb on + strand, within HGI48_RS10310 at 2225.743 kb on - strand, within HGI48_RS10310 at 2226.118 kb on + strand at 2226.120 kb on + strand, within HGI48_RS10310 at 2226.120 kb on + strand, within HGI48_RS10310 at 2226.120 kb on + strand, within HGI48_RS10310 at 2226.120 kb on + strand, within HGI48_RS10310 at 2226.121 kb on - strand at 2226.285 kb on + strand at 2226.285 kb on + strand at 2226.285 kb on + strand at 2226.286 kb on - strand at 2226.286 kb on - strand at 2226.286 kb on - strand at 2226.312 kb on + strand at 2226.312 kb on + strand at 2226.312 kb on + strand at 2226.312 kb on + strand at 2226.313 kb on - strand at 2226.403 kb on + strand at 2226.454 kb on + strand, within HGI48_RS10315 at 2226.455 kb on - strand, within HGI48_RS10315 at 2226.494 kb on + strand, within HGI48_RS10315 at 2226.694 kb on - strand, within HGI48_RS10315 at 2226.694 kb on - strand at 2226.694 kb on - strand at 2226.804 kb on + strand at 2226.810 kb on + strand at 2226.965 kb on + strand, within HGI48_RS10320 at 2226.965 kb on + strand at 2227.215 kb on - strand, within HGI48_RS10320 at 2227.273 kb on + strand at 2227.274 kb on - strand, within HGI48_RS10320
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 2,223,546 - +0.4 2,223,606 + +0.4 2,223,695 - -0.0 2,223,755 - +0.1 2,223,774 - +0.7 2,223,991 - HGI48_RS10305 0.27 +0.1 2,224,143 + HGI48_RS10305 0.54 -0.1 2,224,318 + HGI48_RS10305 0.86 -0.3 2,224,318 + HGI48_RS10305 0.86 -0.4 2,224,319 - HGI48_RS10305 0.86 -1.6 2,224,338 + HGI48_RS10305 0.89 +0.5 2,224,368 + -0.3 2,224,388 - -2.7 2,224,388 - +0.1 2,224,388 - -0.7 2,224,388 - +0.1 2,224,394 - -0.1 2,224,403 - -1.9 2,224,567 - -1.6 2,224,639 + -1.6 2,224,640 - +1.6 2,224,640 - -0.8 2,224,809 - HGI48_RS10310 0.17 +0.0 2,224,831 + HGI48_RS10310 0.18 -0.1 2,225,017 + HGI48_RS10310 0.28 -0.3 2,225,017 + HGI48_RS10310 0.28 -1.0 2,225,018 - -0.6 2,225,018 - HGI48_RS10310 0.28 -1.6 2,225,018 - HGI48_RS10310 0.28 +0.4 2,225,019 + HGI48_RS10310 0.28 -0.1 2,225,019 + HGI48_RS10310 0.28 -0.7 2,225,019 + HGI48_RS10310 0.28 +0.7 2,225,020 - HGI48_RS10310 0.28 +0.6 2,225,314 + HGI48_RS10310 0.43 -0.8 2,225,315 - HGI48_RS10310 0.43 -0.3 2,225,316 + HGI48_RS10310 0.43 -1.1 2,225,317 - HGI48_RS10310 0.43 -3.1 2,225,317 - +2.0 2,225,317 - HGI48_RS10310 0.43 -2.1 2,225,323 - HGI48_RS10310 0.44 -1.9 2,225,437 + HGI48_RS10310 0.50 -2.1 2,225,457 + HGI48_RS10310 0.51 -0.1 2,225,458 - HGI48_RS10310 0.51 -0.9 2,225,458 - HGI48_RS10310 0.51 -0.4 2,225,686 + HGI48_RS10310 0.63 -2.6 2,225,686 + -0.1 2,225,707 + HGI48_RS10310 0.64 +0.4 2,225,707 + HGI48_RS10310 0.64 +0.4 2,225,708 - HGI48_RS10310 0.64 +1.0 2,225,742 + HGI48_RS10310 0.66 -1.4 2,225,743 - HGI48_RS10310 0.66 -0.6 2,226,118 + -0.6 2,226,120 + HGI48_RS10310 0.85 +0.4 2,226,120 + HGI48_RS10310 0.85 -0.4 2,226,120 + HGI48_RS10310 0.85 -0.6 2,226,120 + HGI48_RS10310 0.85 -0.6 2,226,121 - -1.1 2,226,285 + -2.6 2,226,285 + -1.1 2,226,285 + -0.9 2,226,286 - -2.0 2,226,286 - -1.9 2,226,286 - +0.4 2,226,312 + +0.9 2,226,312 + -2.6 2,226,312 + -0.3 2,226,312 + +0.2 2,226,313 - +0.3 2,226,403 + -0.9 2,226,454 + HGI48_RS10315 0.11 +0.0 2,226,455 - HGI48_RS10315 0.11 -0.6 2,226,494 + HGI48_RS10315 0.21 -0.7 2,226,694 - HGI48_RS10315 0.71 +1.4 2,226,694 - -1.1 2,226,694 - +0.4 2,226,804 + -1.6 2,226,810 + -0.5 2,226,965 + HGI48_RS10320 0.14 -0.8 2,226,965 + -0.1 2,227,215 - HGI48_RS10320 0.45 -0.1 2,227,273 + +0.4 2,227,274 - HGI48_RS10320 0.52 +0.0
Or see this region's nucleotide sequence