Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS08715 and HGI48_RS08720 overlap by 4 nucleotides HGI48_RS08720 and HGI48_RS08725 are separated by 3 nucleotides HGI48_RS08725 and HGI48_RS08730 are separated by 244 nucleotides HGI48_RS08730 and HGI48_RS08735 are separated by 34 nucleotides
HGI48_RS08715: HGI48_RS08715 - cyclic pyranopterin monophosphate synthase MoaC, at 1,874,269 to 1,874,751
_RS08715
HGI48_RS08720: HGI48_RS08720 - molybdopterin synthase sulfur carrier subunit, at 1,874,748 to 1,874,993
_RS08720
HGI48_RS08725: HGI48_RS08725 - molybdopterin synthase catalytic subunit MoaE, at 1,874,997 to 1,875,446
_RS08725
HGI48_RS08730: HGI48_RS08730 - iron ABC transporter substrate-binding protein, at 1,875,691 to 1,876,707
_RS08730
HGI48_RS08735: HGI48_RS08735 - iron ABC transporter permease, at 1,876,742 to 1,878,334
_RS08735
Position (kb)
1875
1876
1877 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1874.753 kb on + strand at 1874.753 kb on + strand at 1874.754 kb on - strand at 1874.820 kb on + strand, within HGI48_RS08720 at 1874.820 kb on + strand at 1875.008 kb on + strand at 1875.008 kb on + strand at 1875.008 kb on + strand at 1875.008 kb on + strand at 1875.008 kb on + strand at 1875.008 kb on + strand at 1875.009 kb on - strand at 1875.009 kb on - strand at 1875.009 kb on - strand at 1875.009 kb on - strand at 1875.049 kb on - strand, within HGI48_RS08725 at 1875.049 kb on - strand, within HGI48_RS08725 at 1875.106 kb on + strand, within HGI48_RS08725 at 1875.106 kb on + strand, within HGI48_RS08725 at 1875.107 kb on - strand, within HGI48_RS08725 at 1875.107 kb on - strand, within HGI48_RS08725 at 1875.107 kb on - strand, within HGI48_RS08725 at 1875.342 kb on + strand, within HGI48_RS08725 at 1875.460 kb on + strand at 1875.498 kb on + strand at 1875.538 kb on - strand at 1875.552 kb on + strand at 1875.569 kb on + strand at 1875.648 kb on + strand at 1875.649 kb on - strand at 1875.657 kb on - strand at 1875.657 kb on - strand at 1875.662 kb on + strand at 1875.663 kb on - strand at 1875.670 kb on + strand at 1875.690 kb on + strand at 1875.703 kb on - strand at 1875.704 kb on + strand at 1875.704 kb on + strand at 1875.705 kb on - strand at 1875.705 kb on - strand at 1875.705 kb on - strand at 1875.705 kb on - strand at 1875.705 kb on - strand at 1875.705 kb on - strand at 1875.705 kb on - strand at 1875.705 kb on - strand at 1875.705 kb on - strand at 1875.786 kb on + strand at 1875.786 kb on + strand at 1875.786 kb on + strand at 1875.787 kb on - strand at 1875.787 kb on - strand at 1875.796 kb on + strand, within HGI48_RS08730 at 1875.796 kb on + strand, within HGI48_RS08730 at 1875.796 kb on + strand, within HGI48_RS08730 at 1875.843 kb on + strand at 1876.040 kb on - strand, within HGI48_RS08730 at 1876.096 kb on + strand, within HGI48_RS08730 at 1876.112 kb on + strand, within HGI48_RS08730 at 1876.209 kb on + strand, within HGI48_RS08730 at 1876.210 kb on - strand, within HGI48_RS08730 at 1876.292 kb on - strand, within HGI48_RS08730 at 1876.292 kb on - strand, within HGI48_RS08730 at 1876.292 kb on - strand, within HGI48_RS08730 at 1876.293 kb on + strand, within HGI48_RS08730 at 1876.294 kb on - strand, within HGI48_RS08730 at 1876.354 kb on + strand at 1876.354 kb on + strand, within HGI48_RS08730 at 1876.354 kb on + strand, within HGI48_RS08730 at 1876.355 kb on - strand, within HGI48_RS08730 at 1876.355 kb on - strand, within HGI48_RS08730 at 1876.355 kb on - strand, within HGI48_RS08730 at 1876.355 kb on - strand, within HGI48_RS08730 at 1876.355 kb on - strand, within HGI48_RS08730 at 1876.360 kb on + strand, within HGI48_RS08730 at 1876.360 kb on + strand, within HGI48_RS08730 at 1876.360 kb on + strand, within HGI48_RS08730 at 1876.361 kb on - strand, within HGI48_RS08730 at 1876.361 kb on - strand at 1876.363 kb on + strand at 1876.408 kb on - strand, within HGI48_RS08730 at 1876.417 kb on + strand, within HGI48_RS08730 at 1876.418 kb on - strand at 1876.418 kb on - strand, within HGI48_RS08730 at 1876.418 kb on - strand, within HGI48_RS08730 at 1876.418 kb on - strand, within HGI48_RS08730 at 1876.491 kb on + strand, within HGI48_RS08730 at 1876.491 kb on + strand, within HGI48_RS08730 at 1876.492 kb on - strand, within HGI48_RS08730 at 1876.492 kb on - strand, within HGI48_RS08730 at 1876.492 kb on - strand, within HGI48_RS08730 at 1876.492 kb on - strand, within HGI48_RS08730 at 1876.573 kb on + strand, within HGI48_RS08730 at 1876.573 kb on + strand, within HGI48_RS08730 at 1876.705 kb on + strand at 1876.706 kb on - strand at 1876.734 kb on + strand at 1876.734 kb on + strand at 1876.735 kb on - strand at 1876.735 kb on - strand at 1876.741 kb on + strand at 1876.741 kb on + strand at 1876.741 kb on + strand at 1876.741 kb on + strand at 1876.741 kb on + strand at 1876.742 kb on - strand at 1876.742 kb on - strand at 1876.924 kb on - strand, within HGI48_RS08735 at 1876.966 kb on + strand, within HGI48_RS08735 at 1876.967 kb on - strand, within HGI48_RS08735 at 1876.967 kb on - strand, within HGI48_RS08735 at 1876.967 kb on - strand, within HGI48_RS08735 at 1876.967 kb on - strand, within HGI48_RS08735 at 1876.974 kb on + strand, within HGI48_RS08735 at 1876.975 kb on - strand at 1877.121 kb on - strand, within HGI48_RS08735 at 1877.189 kb on + strand at 1877.190 kb on - strand, within HGI48_RS08735 at 1877.190 kb on - strand, within HGI48_RS08735 at 1877.201 kb on - strand, within HGI48_RS08735 at 1877.201 kb on - strand, within HGI48_RS08735 at 1877.201 kb on - strand, within HGI48_RS08735 at 1877.204 kb on + strand, within HGI48_RS08735 at 1877.204 kb on + strand, within HGI48_RS08735 at 1877.204 kb on + strand, within HGI48_RS08735 at 1877.204 kb on + strand, within HGI48_RS08735 at 1877.205 kb on - strand, within HGI48_RS08735 at 1877.205 kb on - strand, within HGI48_RS08735 at 1877.205 kb on - strand, within HGI48_RS08735 at 1877.373 kb on - strand, within HGI48_RS08735 at 1877.438 kb on - strand, within HGI48_RS08735 at 1877.438 kb on - strand, within HGI48_RS08735
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 1,874,753 + -0.8 1,874,753 + +1.5 1,874,754 - -0.7 1,874,820 + HGI48_RS08720 0.29 +0.0 1,874,820 + +1.3 1,875,008 + -0.8 1,875,008 + +0.3 1,875,008 + -0.5 1,875,008 + -0.2 1,875,008 + +0.1 1,875,008 + -1.8 1,875,009 - -0.1 1,875,009 - -0.1 1,875,009 - +0.8 1,875,009 - +1.5 1,875,049 - HGI48_RS08725 0.12 +1.1 1,875,049 - HGI48_RS08725 0.12 -0.7 1,875,106 + HGI48_RS08725 0.24 -2.0 1,875,106 + HGI48_RS08725 0.24 -0.0 1,875,107 - HGI48_RS08725 0.24 -0.9 1,875,107 - HGI48_RS08725 0.24 +0.5 1,875,107 - HGI48_RS08725 0.24 -1.8 1,875,342 + HGI48_RS08725 0.77 -0.1 1,875,460 + +1.3 1,875,498 + -1.5 1,875,538 - -0.9 1,875,552 + -2.5 1,875,569 + +1.3 1,875,648 + -0.7 1,875,649 - -1.5 1,875,657 - -2.3 1,875,657 - -1.5 1,875,662 + +1.4 1,875,663 - -1.5 1,875,670 + +1.0 1,875,690 + -0.9 1,875,703 - -0.0 1,875,704 + -0.0 1,875,704 + -1.6 1,875,705 - -0.0 1,875,705 - -0.5 1,875,705 - -1.9 1,875,705 - -0.8 1,875,705 - +0.3 1,875,705 - +0.3 1,875,705 - -1.8 1,875,705 - +1.1 1,875,705 - -1.6 1,875,786 + -1.0 1,875,786 + -1.0 1,875,786 + +0.7 1,875,787 - +0.1 1,875,787 - -2.0 1,875,796 + HGI48_RS08730 0.10 -2.9 1,875,796 + HGI48_RS08730 0.10 +0.1 1,875,796 + HGI48_RS08730 0.10 +0.2 1,875,843 + -1.0 1,876,040 - HGI48_RS08730 0.34 -2.6 1,876,096 + HGI48_RS08730 0.40 -0.8 1,876,112 + HGI48_RS08730 0.41 -0.5 1,876,209 + HGI48_RS08730 0.51 -1.5 1,876,210 - HGI48_RS08730 0.51 +0.5 1,876,292 - HGI48_RS08730 0.59 -0.7 1,876,292 - HGI48_RS08730 0.59 -1.0 1,876,292 - HGI48_RS08730 0.59 -1.3 1,876,293 + HGI48_RS08730 0.59 -0.0 1,876,294 - HGI48_RS08730 0.59 -0.9 1,876,354 + +1.3 1,876,354 + HGI48_RS08730 0.65 -0.2 1,876,354 + HGI48_RS08730 0.65 -0.2 1,876,355 - HGI48_RS08730 0.65 -0.2 1,876,355 - HGI48_RS08730 0.65 -1.0 1,876,355 - HGI48_RS08730 0.65 -1.3 1,876,355 - HGI48_RS08730 0.65 -0.8 1,876,355 - HGI48_RS08730 0.65 -0.5 1,876,360 + HGI48_RS08730 0.66 -2.6 1,876,360 + HGI48_RS08730 0.66 -2.8 1,876,360 + HGI48_RS08730 0.66 -0.5 1,876,361 - HGI48_RS08730 0.66 -2.3 1,876,361 - +0.5 1,876,363 + +2.0 1,876,408 - HGI48_RS08730 0.71 -2.3 1,876,417 + HGI48_RS08730 0.71 -1.5 1,876,418 - -1.0 1,876,418 - HGI48_RS08730 0.71 -2.0 1,876,418 - HGI48_RS08730 0.71 -1.8 1,876,418 - HGI48_RS08730 0.71 -2.8 1,876,491 + HGI48_RS08730 0.79 +0.5 1,876,491 + HGI48_RS08730 0.79 -0.9 1,876,492 - HGI48_RS08730 0.79 -1.5 1,876,492 - HGI48_RS08730 0.79 -1.3 1,876,492 - HGI48_RS08730 0.79 -1.8 1,876,492 - HGI48_RS08730 0.79 -0.6 1,876,573 + HGI48_RS08730 0.87 +0.1 1,876,573 + HGI48_RS08730 0.87 -1.9 1,876,705 + -2.3 1,876,706 - -2.6 1,876,734 + +0.1 1,876,734 + -0.4 1,876,735 - +0.4 1,876,735 - -1.0 1,876,741 + -0.0 1,876,741 + +0.3 1,876,741 + +0.5 1,876,741 + +0.5 1,876,741 + +0.3 1,876,742 - -0.3 1,876,742 - +0.8 1,876,924 - HGI48_RS08735 0.11 -0.5 1,876,966 + HGI48_RS08735 0.14 -0.8 1,876,967 - HGI48_RS08735 0.14 -0.5 1,876,967 - HGI48_RS08735 0.14 -0.5 1,876,967 - HGI48_RS08735 0.14 -1.1 1,876,967 - HGI48_RS08735 0.14 -1.0 1,876,974 + HGI48_RS08735 0.15 -1.4 1,876,975 - +0.5 1,877,121 - HGI48_RS08735 0.24 -2.4 1,877,189 + -0.0 1,877,190 - HGI48_RS08735 0.28 -2.3 1,877,190 - HGI48_RS08735 0.28 +0.5 1,877,201 - HGI48_RS08735 0.29 +0.8 1,877,201 - HGI48_RS08735 0.29 -1.5 1,877,201 - HGI48_RS08735 0.29 -3.0 1,877,204 + HGI48_RS08735 0.29 -1.3 1,877,204 + HGI48_RS08735 0.29 +1.4 1,877,204 + HGI48_RS08735 0.29 -1.2 1,877,204 + HGI48_RS08735 0.29 -0.9 1,877,205 - HGI48_RS08735 0.29 -0.8 1,877,205 - HGI48_RS08735 0.29 -1.8 1,877,205 - HGI48_RS08735 0.29 -2.5 1,877,373 - HGI48_RS08735 0.40 -1.5 1,877,438 - HGI48_RS08735 0.44 -1.5 1,877,438 - HGI48_RS08735 0.44 -1.8
Or see this region's nucleotide sequence