Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS05190 and HGI48_RS05195 are separated by 12 nucleotides HGI48_RS05195 and HGI48_RS05200 are separated by 204 nucleotides HGI48_RS05200 and HGI48_RS05205 overlap by 11 nucleotides
HGI48_RS05190: HGI48_RS05190 - DNA polymerase III subunit alpha, at 1,087,610 to 1,091,092
_RS05190
HGI48_RS05195: HGI48_RS05195 - acetyl-CoA carboxylase carboxyl transferase subunit alpha, at 1,091,105 to 1,092,064
_RS05195
HGI48_RS05200: HGI48_RS05200 - VOC family protein, at 1,092,269 to 1,092,658
_RS05200
HGI48_RS05205: HGI48_RS05205 - tRNA lysidine(34) synthetase TilS, at 1,092,648 to 1,093,976
_RS05205
Position (kb) 1091
1092
1093 Strain fitness (log2 ratio) -2
-1
0
1 at 1092.140 kb on + strand at 1092.185 kb on + strand at 1092.268 kb on + strand at 1092.268 kb on + strand at 1092.268 kb on + strand at 1092.304 kb on + strand at 1092.317 kb on + strand, within HGI48_RS05200 at 1092.338 kb on + strand, within HGI48_RS05200 at 1092.338 kb on + strand, within HGI48_RS05200 at 1092.590 kb on + strand at 1092.590 kb on + strand at 1092.647 kb on + strand at 1092.648 kb on - strand at 1092.659 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 1,092,140 + +1.3 1,092,185 + -1.1 1,092,268 + -0.9 1,092,268 + +0.7 1,092,268 + -1.9 1,092,304 + +0.0 1,092,317 + HGI48_RS05200 0.12 -1.7 1,092,338 + HGI48_RS05200 0.18 +0.3 1,092,338 + HGI48_RS05200 0.18 -0.5 1,092,590 + -0.9 1,092,590 + -0.3 1,092,647 + +1.4 1,092,648 - -0.9 1,092,659 + -0.9
Or see this region's nucleotide sequence