Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS03400 and HGI48_RS03405 are separated by 79 nucleotides HGI48_RS03405 and HGI48_RS03410 are separated by 273 nucleotides HGI48_RS03410 and HGI48_RS03415 are separated by 73 nucleotides
HGI48_RS03400: HGI48_RS03400 - aminomethyl-transferring glycine dehydrogenase, at 713,660 to 716,533
_RS03400
HGI48_RS03405: HGI48_RS03405 - HD domain-containing protein, at 716,613 to 717,221
_RS03405
HGI48_RS03410: HGI48_RS03410 - hemolysin III family protein, at 717,495 to 718,145
_RS03410
HGI48_RS03415: HGI48_RS03415 - tRNA-modifying protein YgfZ, at 718,219 to 719,199
_RS03415
Position (kb) 716
717
718 Strain fitness (log2 ratio) -2
-1
0
1
2 at 715.616 kb on + strand, within HGI48_RS03400 at 715.617 kb on - strand, within HGI48_RS03400 at 715.637 kb on + strand, within HGI48_RS03400 at 715.668 kb on + strand, within HGI48_RS03400 at 715.669 kb on - strand, within HGI48_RS03400 at 715.669 kb on - strand, within HGI48_RS03400 at 715.669 kb on - strand, within HGI48_RS03400 at 715.679 kb on + strand, within HGI48_RS03400 at 715.679 kb on + strand, within HGI48_RS03400 at 715.680 kb on - strand, within HGI48_RS03400 at 715.694 kb on + strand, within HGI48_RS03400 at 715.694 kb on + strand, within HGI48_RS03400 at 715.695 kb on - strand, within HGI48_RS03400 at 715.695 kb on - strand, within HGI48_RS03400 at 715.732 kb on + strand, within HGI48_RS03400 at 715.732 kb on + strand, within HGI48_RS03400 at 715.732 kb on + strand, within HGI48_RS03400 at 715.733 kb on - strand, within HGI48_RS03400 at 715.745 kb on + strand, within HGI48_RS03400 at 715.745 kb on + strand, within HGI48_RS03400 at 715.942 kb on + strand, within HGI48_RS03400 at 715.942 kb on + strand, within HGI48_RS03400 at 715.964 kb on + strand, within HGI48_RS03400 at 715.964 kb on + strand, within HGI48_RS03400 at 715.964 kb on + strand, within HGI48_RS03400 at 715.964 kb on + strand, within HGI48_RS03400 at 715.964 kb on + strand at 715.965 kb on - strand, within HGI48_RS03400 at 715.965 kb on - strand, within HGI48_RS03400 at 715.965 kb on - strand, within HGI48_RS03400 at 716.030 kb on + strand, within HGI48_RS03400 at 716.030 kb on + strand, within HGI48_RS03400 at 716.030 kb on + strand, within HGI48_RS03400 at 716.031 kb on - strand, within HGI48_RS03400 at 716.031 kb on - strand, within HGI48_RS03400 at 716.031 kb on - strand, within HGI48_RS03400 at 716.058 kb on - strand, within HGI48_RS03400 at 716.069 kb on + strand, within HGI48_RS03400 at 716.069 kb on + strand, within HGI48_RS03400 at 716.069 kb on + strand, within HGI48_RS03400 at 716.069 kb on + strand, within HGI48_RS03400 at 716.069 kb on + strand, within HGI48_RS03400 at 716.070 kb on - strand, within HGI48_RS03400 at 716.070 kb on - strand, within HGI48_RS03400 at 716.070 kb on - strand, within HGI48_RS03400 at 716.070 kb on - strand, within HGI48_RS03400 at 716.177 kb on + strand, within HGI48_RS03400 at 716.304 kb on + strand at 716.304 kb on + strand at 716.304 kb on + strand at 716.304 kb on + strand at 716.305 kb on - strand at 716.305 kb on - strand at 716.352 kb on + strand at 716.447 kb on + strand at 716.448 kb on - strand at 716.448 kb on - strand at 716.491 kb on + strand at 716.492 kb on - strand at 716.532 kb on - strand at 716.545 kb on - strand at 716.594 kb on + strand at 716.595 kb on - strand at 716.614 kb on + strand at 716.614 kb on + strand at 716.614 kb on + strand at 716.614 kb on + strand at 716.615 kb on - strand at 716.615 kb on - strand at 716.615 kb on - strand at 716.615 kb on - strand at 716.615 kb on - strand at 716.615 kb on - strand at 716.836 kb on + strand, within HGI48_RS03405 at 716.837 kb on - strand, within HGI48_RS03405 at 716.950 kb on + strand, within HGI48_RS03405 at 716.950 kb on + strand, within HGI48_RS03405 at 716.950 kb on + strand, within HGI48_RS03405 at 716.951 kb on - strand, within HGI48_RS03405 at 716.951 kb on - strand, within HGI48_RS03405 at 716.951 kb on - strand, within HGI48_RS03405 at 716.958 kb on + strand, within HGI48_RS03405 at 716.959 kb on - strand, within HGI48_RS03405 at 716.959 kb on - strand at 717.040 kb on + strand, within HGI48_RS03405 at 717.240 kb on - strand at 717.240 kb on - strand at 717.250 kb on + strand at 717.250 kb on + strand at 717.250 kb on + strand at 717.250 kb on + strand at 717.251 kb on - strand at 717.251 kb on - strand at 717.251 kb on - strand at 717.251 kb on - strand at 717.256 kb on - strand at 717.270 kb on - strand at 717.288 kb on + strand at 717.289 kb on - strand at 717.289 kb on - strand at 717.289 kb on - strand at 717.289 kb on - strand at 717.330 kb on + strand at 717.330 kb on + strand at 717.434 kb on - strand at 717.434 kb on - strand at 717.434 kb on - strand at 717.434 kb on - strand at 717.434 kb on - strand at 717.434 kb on - strand at 717.444 kb on + strand at 717.445 kb on - strand at 717.467 kb on + strand at 717.467 kb on + strand at 717.468 kb on - strand at 717.468 kb on - strand at 717.524 kb on + strand at 717.524 kb on + strand at 717.524 kb on + strand at 717.525 kb on - strand at 717.525 kb on - strand at 717.525 kb on - strand at 717.551 kb on + strand at 717.551 kb on + strand at 717.551 kb on + strand at 717.551 kb on + strand at 717.552 kb on - strand at 717.598 kb on + strand, within HGI48_RS03410 at 717.598 kb on + strand, within HGI48_RS03410 at 717.599 kb on - strand at 717.599 kb on - strand, within HGI48_RS03410 at 717.599 kb on - strand, within HGI48_RS03410 at 717.599 kb on - strand, within HGI48_RS03410 at 717.599 kb on - strand, within HGI48_RS03410 at 717.601 kb on + strand, within HGI48_RS03410 at 717.651 kb on + strand, within HGI48_RS03410 at 717.652 kb on - strand, within HGI48_RS03410 at 717.652 kb on - strand, within HGI48_RS03410 at 717.679 kb on + strand, within HGI48_RS03410 at 717.680 kb on - strand, within HGI48_RS03410 at 717.702 kb on + strand, within HGI48_RS03410 at 717.703 kb on - strand, within HGI48_RS03410 at 717.761 kb on + strand, within HGI48_RS03410 at 717.761 kb on + strand, within HGI48_RS03410 at 717.762 kb on - strand, within HGI48_RS03410 at 717.765 kb on + strand, within HGI48_RS03410 at 717.765 kb on + strand, within HGI48_RS03410 at 717.765 kb on + strand at 717.765 kb on + strand, within HGI48_RS03410 at 717.766 kb on - strand at 717.766 kb on - strand, within HGI48_RS03410 at 717.772 kb on + strand, within HGI48_RS03410 at 717.772 kb on + strand, within HGI48_RS03410 at 717.786 kb on + strand, within HGI48_RS03410 at 717.786 kb on + strand, within HGI48_RS03410 at 717.786 kb on + strand, within HGI48_RS03410 at 717.786 kb on + strand, within HGI48_RS03410 at 717.786 kb on + strand, within HGI48_RS03410 at 717.786 kb on + strand, within HGI48_RS03410 at 717.786 kb on + strand, within HGI48_RS03410 at 717.786 kb on + strand, within HGI48_RS03410 at 717.786 kb on + strand, within HGI48_RS03410 at 717.787 kb on - strand, within HGI48_RS03410 at 717.787 kb on - strand, within HGI48_RS03410 at 717.787 kb on - strand, within HGI48_RS03410 at 717.788 kb on + strand, within HGI48_RS03410 at 717.788 kb on + strand, within HGI48_RS03410 at 717.812 kb on + strand, within HGI48_RS03410 at 717.813 kb on - strand at 717.845 kb on + strand, within HGI48_RS03410 at 717.845 kb on + strand, within HGI48_RS03410 at 717.845 kb on + strand, within HGI48_RS03410 at 717.846 kb on - strand at 717.924 kb on + strand, within HGI48_RS03410 at 717.924 kb on + strand, within HGI48_RS03410 at 717.961 kb on - strand, within HGI48_RS03410 at 717.961 kb on - strand at 718.023 kb on + strand, within HGI48_RS03410 at 718.023 kb on + strand, within HGI48_RS03410 at 718.024 kb on - strand, within HGI48_RS03410 at 718.026 kb on - strand, within HGI48_RS03410 at 718.045 kb on - strand, within HGI48_RS03410 at 718.128 kb on + strand at 718.128 kb on + strand at 718.128 kb on + strand at 718.128 kb on + strand at 718.128 kb on + strand at 718.128 kb on + strand at 718.129 kb on - strand at 718.129 kb on - strand at 718.129 kb on - strand at 718.134 kb on + strand at 718.134 kb on + strand at 718.134 kb on + strand at 718.134 kb on + strand at 718.134 kb on + strand at 718.135 kb on - strand at 718.135 kb on - strand at 718.135 kb on - strand at 718.135 kb on - strand at 718.138 kb on + strand at 718.138 kb on + strand at 718.138 kb on + strand at 718.139 kb on - strand at 718.139 kb on - strand at 718.139 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 715,616 + HGI48_RS03400 0.68 +1.6 715,617 - HGI48_RS03400 0.68 -1.7 715,637 + HGI48_RS03400 0.69 -2.0 715,668 + HGI48_RS03400 0.70 -1.0 715,669 - HGI48_RS03400 0.70 -2.5 715,669 - HGI48_RS03400 0.70 -1.0 715,669 - HGI48_RS03400 0.70 -2.0 715,679 + HGI48_RS03400 0.70 -0.6 715,679 + HGI48_RS03400 0.70 +0.4 715,680 - HGI48_RS03400 0.70 +0.1 715,694 + HGI48_RS03400 0.71 -0.7 715,694 + HGI48_RS03400 0.71 -0.5 715,695 - HGI48_RS03400 0.71 -0.4 715,695 - HGI48_RS03400 0.71 -1.1 715,732 + HGI48_RS03400 0.72 +0.1 715,732 + HGI48_RS03400 0.72 -1.5 715,732 + HGI48_RS03400 0.72 -0.5 715,733 - HGI48_RS03400 0.72 -0.3 715,745 + HGI48_RS03400 0.73 +0.8 715,745 + HGI48_RS03400 0.73 -1.7 715,942 + HGI48_RS03400 0.79 +0.9 715,942 + HGI48_RS03400 0.79 +1.1 715,964 + HGI48_RS03400 0.80 -0.4 715,964 + HGI48_RS03400 0.80 -1.5 715,964 + HGI48_RS03400 0.80 -0.3 715,964 + HGI48_RS03400 0.80 +0.1 715,964 + +0.8 715,965 - HGI48_RS03400 0.80 -0.6 715,965 - HGI48_RS03400 0.80 +0.0 715,965 - HGI48_RS03400 0.80 -0.2 716,030 + HGI48_RS03400 0.82 -0.5 716,030 + HGI48_RS03400 0.82 -0.2 716,030 + HGI48_RS03400 0.82 -0.5 716,031 - HGI48_RS03400 0.82 -2.2 716,031 - HGI48_RS03400 0.82 +0.1 716,031 - HGI48_RS03400 0.82 +2.0 716,058 - HGI48_RS03400 0.83 +0.1 716,069 + HGI48_RS03400 0.84 +0.7 716,069 + HGI48_RS03400 0.84 -2.5 716,069 + HGI48_RS03400 0.84 -1.3 716,069 + HGI48_RS03400 0.84 -0.9 716,069 + HGI48_RS03400 0.84 +1.4 716,070 - HGI48_RS03400 0.84 +0.2 716,070 - HGI48_RS03400 0.84 +0.1 716,070 - HGI48_RS03400 0.84 +0.8 716,070 - HGI48_RS03400 0.84 +0.6 716,177 + HGI48_RS03400 0.88 -0.0 716,304 + +0.1 716,304 + -1.2 716,304 + -2.6 716,304 + -1.2 716,305 - -0.5 716,305 - +0.3 716,352 + -0.9 716,447 + -0.6 716,448 - +0.2 716,448 - -1.7 716,491 + -2.2 716,492 - -1.7 716,532 - +0.4 716,545 - +0.1 716,594 + +0.8 716,595 - +1.3 716,614 + +0.3 716,614 + +0.1 716,614 + +1.7 716,614 + +0.8 716,615 - -0.5 716,615 - +0.8 716,615 - -0.2 716,615 - -0.3 716,615 - +0.2 716,615 - -0.6 716,836 + HGI48_RS03405 0.37 -0.4 716,837 - HGI48_RS03405 0.37 -0.4 716,950 + HGI48_RS03405 0.55 -1.1 716,950 + HGI48_RS03405 0.55 +0.7 716,950 + HGI48_RS03405 0.55 +0.5 716,951 - HGI48_RS03405 0.56 -2.2 716,951 - HGI48_RS03405 0.56 +0.1 716,951 - HGI48_RS03405 0.56 -0.4 716,958 + HGI48_RS03405 0.57 +0.1 716,959 - HGI48_RS03405 0.57 -0.5 716,959 - +0.8 717,040 + HGI48_RS03405 0.70 +0.5 717,240 - +0.1 717,240 - -0.7 717,250 + -1.2 717,250 + +0.4 717,250 + +0.2 717,250 + +0.1 717,251 - +0.1 717,251 - -0.3 717,251 - +0.2 717,251 - -1.2 717,256 - -0.7 717,270 - -0.2 717,288 + -1.2 717,289 - +1.7 717,289 - +1.3 717,289 - -0.9 717,289 - +0.4 717,330 + -0.6 717,330 + -1.2 717,434 - -0.5 717,434 - -1.5 717,434 - -0.1 717,434 - -0.5 717,434 - +0.1 717,434 - -0.7 717,444 + -0.6 717,445 - +0.3 717,467 + +0.1 717,467 + -1.3 717,468 - +0.9 717,468 - -1.7 717,524 + +0.8 717,524 + +1.8 717,524 + -0.3 717,525 - +0.6 717,525 - -0.2 717,525 - -0.4 717,551 + +0.8 717,551 + +1.2 717,551 + +0.1 717,551 + -0.8 717,552 - -1.2 717,598 + HGI48_RS03410 0.16 -0.2 717,598 + HGI48_RS03410 0.16 +0.7 717,599 - +0.3 717,599 - HGI48_RS03410 0.16 -2.0 717,599 - HGI48_RS03410 0.16 +1.1 717,599 - HGI48_RS03410 0.16 -0.5 717,599 - HGI48_RS03410 0.16 -1.0 717,601 + HGI48_RS03410 0.16 -1.5 717,651 + HGI48_RS03410 0.24 -0.2 717,652 - HGI48_RS03410 0.24 -0.1 717,652 - HGI48_RS03410 0.24 -0.2 717,679 + HGI48_RS03410 0.28 -1.6 717,680 - HGI48_RS03410 0.28 +1.2 717,702 + HGI48_RS03410 0.32 -0.5 717,703 - HGI48_RS03410 0.32 -1.2 717,761 + HGI48_RS03410 0.41 -1.5 717,761 + HGI48_RS03410 0.41 +0.1 717,762 - HGI48_RS03410 0.41 -0.4 717,765 + HGI48_RS03410 0.41 +1.5 717,765 + HGI48_RS03410 0.41 +0.4 717,765 + +2.3 717,765 + HGI48_RS03410 0.41 -1.2 717,766 - -0.7 717,766 - HGI48_RS03410 0.42 -2.4 717,772 + HGI48_RS03410 0.43 +0.0 717,772 + HGI48_RS03410 0.43 -0.2 717,786 + HGI48_RS03410 0.45 +0.8 717,786 + HGI48_RS03410 0.45 +0.3 717,786 + HGI48_RS03410 0.45 -1.5 717,786 + HGI48_RS03410 0.45 +0.4 717,786 + HGI48_RS03410 0.45 +1.8 717,786 + HGI48_RS03410 0.45 -0.1 717,786 + HGI48_RS03410 0.45 -1.3 717,786 + HGI48_RS03410 0.45 +1.3 717,786 + HGI48_RS03410 0.45 +0.0 717,787 - HGI48_RS03410 0.45 +0.8 717,787 - HGI48_RS03410 0.45 -2.0 717,787 - HGI48_RS03410 0.45 -0.5 717,788 + HGI48_RS03410 0.45 -0.5 717,788 + HGI48_RS03410 0.45 -0.2 717,812 + HGI48_RS03410 0.49 -2.2 717,813 - +1.8 717,845 + HGI48_RS03410 0.54 +0.5 717,845 + HGI48_RS03410 0.54 +0.1 717,845 + HGI48_RS03410 0.54 +0.5 717,846 - -0.7 717,924 + HGI48_RS03410 0.66 -1.3 717,924 + HGI48_RS03410 0.66 -0.3 717,961 - HGI48_RS03410 0.72 -0.7 717,961 - +0.8 718,023 + HGI48_RS03410 0.81 -0.7 718,023 + HGI48_RS03410 0.81 -1.2 718,024 - HGI48_RS03410 0.81 -0.2 718,026 - HGI48_RS03410 0.82 -0.7 718,045 - HGI48_RS03410 0.84 -0.3 718,128 + -1.7 718,128 + +0.2 718,128 + +2.1 718,128 + -0.3 718,128 + -1.5 718,128 + +1.4 718,129 - +0.9 718,129 - +0.2 718,129 - +0.6 718,134 + +0.6 718,134 + +0.1 718,134 + -0.8 718,134 + -0.9 718,134 + +0.3 718,135 - -0.4 718,135 - +0.7 718,135 - +1.8 718,135 - -1.5 718,138 + -0.4 718,138 + -0.5 718,138 + +1.1 718,139 - -1.5 718,139 - -1.7 718,139 - +0.4
Or see this region's nucleotide sequence