Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS03215 and HGI48_RS03220 are separated by 54 nucleotides HGI48_RS03220 and HGI48_RS03225 are separated by 59 nucleotides HGI48_RS03225 and HGI48_RS03230 are separated by 218 nucleotides HGI48_RS03230 and HGI48_RS03235 are separated by 21 nucleotides HGI48_RS03235 and HGI48_RS03240 are separated by 25 nucleotides
HGI48_RS03215: HGI48_RS03215 - preprotein translocase subunit SecG, at 680,366 to 680,701
_RS03215
HGI48_RS03220: HGI48_RS03220 - tRNA-Leu, at 680,756 to 680,842
_RS03220
HGI48_RS03225: HGI48_RS03225 - tRNA-Met, at 680,902 to 680,978
_RS03225
HGI48_RS03230: HGI48_RS03230 - ribosome maturation factor RimP, at 681,197 to 681,649
_RS03230
HGI48_RS03235: HGI48_RS03235 - transcription termination/antitermination protein NusA, at 681,671 to 683,161
_RS03235
HGI48_RS03240: HGI48_RS03240 - translation initiation factor IF-2, at 683,187 to 685,904
_RS03240
Position (kb) 681
682
683
684 Strain fitness (log2 ratio) -2
-1
0
1 at 680.695 kb on + strand at 680.695 kb on + strand at 680.740 kb on + strand at 680.740 kb on + strand at 680.848 kb on - strand at 680.849 kb on + strand at 680.850 kb on - strand at 680.853 kb on + strand at 680.853 kb on + strand at 680.854 kb on - strand at 680.854 kb on - strand at 680.981 kb on + strand at 680.981 kb on + strand at 681.009 kb on + strand at 681.016 kb on + strand at 681.067 kb on + strand at 681.067 kb on + strand at 681.067 kb on + strand at 681.067 kb on + strand at 681.071 kb on + strand at 683.008 kb on + strand, within HGI48_RS03235 at 683.131 kb on + strand at 683.131 kb on + strand at 683.131 kb on + strand at 683.169 kb on + strand at 683.169 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 680,695 + -1.1 680,695 + -0.5 680,740 + -1.0 680,740 + +0.1 680,848 - +0.4 680,849 + -0.1 680,850 - -1.6 680,853 + -1.8 680,853 + +0.4 680,854 - +0.2 680,854 - +0.9 680,981 + +0.5 680,981 + +1.1 681,009 + +0.4 681,016 + -1.1 681,067 + +0.4 681,067 + -0.6 681,067 + -0.3 681,067 + -2.1 681,071 + -1.1 683,008 + HGI48_RS03235 0.90 +1.4 683,131 + +1.7 683,131 + -0.5 683,131 + -0.5 683,169 + -1.5 683,169 + -1.0
Or see this region's nucleotide sequence