Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01535 and HGI48_RS01540 are separated by 15 nucleotides HGI48_RS01540 and HGI48_RS01545 are separated by 317 nucleotides HGI48_RS01545 and HGI48_RS01550 are separated by 4 nucleotides HGI48_RS01550 and HGI48_RS01555 are separated by 98 nucleotides
HGI48_RS01535: HGI48_RS01535 - 50S ribosomal protein L13, at 336,616 to 337,044
_RS01535
HGI48_RS01540: HGI48_RS01540 - 30S ribosomal protein S9, at 337,060 to 337,452
_RS01540
HGI48_RS01545: HGI48_RS01545 - stringent starvation protein A, at 337,770 to 338,411
_RS01545
HGI48_RS01550: HGI48_RS01550 - ClpXP protease specificity-enhancing factor, at 338,416 to 338,913
_RS01550
HGI48_RS01555: HGI48_RS01555 - glutamate synthase small subunit, at 339,012 to 340,430
_RS01555
Position (kb) 337
338
339 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 337.524 kb on + strand at 337.610 kb on + strand at 337.611 kb on - strand at 337.615 kb on + strand at 337.616 kb on - strand at 337.653 kb on + strand at 337.653 kb on + strand at 337.722 kb on + strand at 337.722 kb on + strand at 337.722 kb on + strand at 337.723 kb on - strand at 337.754 kb on + strand at 337.902 kb on - strand, within HGI48_RS01545 at 337.936 kb on - strand, within HGI48_RS01545 at 337.936 kb on - strand at 337.945 kb on + strand, within HGI48_RS01545 at 337.977 kb on + strand, within HGI48_RS01545 at 337.977 kb on + strand at 337.977 kb on + strand, within HGI48_RS01545 at 337.977 kb on + strand, within HGI48_RS01545 at 337.977 kb on + strand, within HGI48_RS01545 at 337.977 kb on + strand, within HGI48_RS01545 at 337.977 kb on + strand, within HGI48_RS01545 at 337.977 kb on + strand, within HGI48_RS01545 at 337.977 kb on + strand, within HGI48_RS01545 at 337.977 kb on + strand, within HGI48_RS01545 at 337.977 kb on + strand, within HGI48_RS01545 at 337.978 kb on - strand, within HGI48_RS01545 at 337.978 kb on - strand, within HGI48_RS01545 at 337.978 kb on - strand, within HGI48_RS01545 at 337.978 kb on - strand, within HGI48_RS01545 at 337.978 kb on - strand, within HGI48_RS01545 at 337.978 kb on - strand, within HGI48_RS01545 at 337.978 kb on - strand, within HGI48_RS01545 at 337.978 kb on - strand at 337.978 kb on - strand, within HGI48_RS01545 at 337.978 kb on - strand at 337.978 kb on - strand at 337.988 kb on + strand, within HGI48_RS01545 at 337.988 kb on + strand, within HGI48_RS01545 at 337.988 kb on + strand, within HGI48_RS01545 at 337.988 kb on + strand, within HGI48_RS01545 at 337.989 kb on - strand, within HGI48_RS01545 at 337.989 kb on - strand, within HGI48_RS01545 at 337.989 kb on - strand, within HGI48_RS01545 at 337.989 kb on - strand, within HGI48_RS01545 at 337.989 kb on - strand, within HGI48_RS01545 at 337.989 kb on - strand, within HGI48_RS01545 at 337.989 kb on - strand, within HGI48_RS01545 at 337.989 kb on - strand, within HGI48_RS01545 at 337.989 kb on - strand, within HGI48_RS01545 at 337.989 kb on - strand, within HGI48_RS01545 at 338.001 kb on + strand, within HGI48_RS01545 at 338.001 kb on + strand, within HGI48_RS01545 at 338.001 kb on + strand, within HGI48_RS01545 at 338.002 kb on - strand, within HGI48_RS01545 at 338.002 kb on - strand, within HGI48_RS01545 at 338.002 kb on - strand, within HGI48_RS01545 at 338.101 kb on - strand at 338.211 kb on + strand, within HGI48_RS01545 at 338.247 kb on + strand, within HGI48_RS01545 at 338.247 kb on + strand, within HGI48_RS01545 at 338.247 kb on + strand, within HGI48_RS01545 at 338.248 kb on - strand, within HGI48_RS01545 at 338.325 kb on + strand, within HGI48_RS01545 at 338.326 kb on - strand, within HGI48_RS01545 at 338.326 kb on - strand at 338.326 kb on - strand, within HGI48_RS01545 at 338.410 kb on - strand at 338.449 kb on + strand at 338.450 kb on - strand at 338.450 kb on - strand at 338.453 kb on + strand at 338.453 kb on + strand at 338.453 kb on + strand at 338.454 kb on - strand at 338.454 kb on - strand at 338.457 kb on - strand at 338.457 kb on - strand at 338.467 kb on + strand, within HGI48_RS01550 at 338.467 kb on + strand, within HGI48_RS01550 at 338.467 kb on + strand, within HGI48_RS01550 at 338.468 kb on - strand, within HGI48_RS01550 at 338.468 kb on - strand, within HGI48_RS01550 at 338.468 kb on - strand, within HGI48_RS01550 at 338.468 kb on - strand, within HGI48_RS01550 at 338.468 kb on - strand, within HGI48_RS01550 at 338.573 kb on + strand, within HGI48_RS01550 at 338.573 kb on + strand, within HGI48_RS01550 at 338.573 kb on + strand, within HGI48_RS01550 at 338.577 kb on + strand, within HGI48_RS01550 at 338.578 kb on - strand, within HGI48_RS01550 at 338.578 kb on - strand, within HGI48_RS01550 at 338.625 kb on - strand, within HGI48_RS01550 at 338.695 kb on + strand, within HGI48_RS01550 at 338.696 kb on - strand, within HGI48_RS01550 at 338.696 kb on - strand, within HGI48_RS01550 at 338.911 kb on + strand at 338.911 kb on + strand at 339.014 kb on - strand at 339.025 kb on + strand at 339.025 kb on + strand at 339.026 kb on - strand at 339.026 kb on - strand at 339.026 kb on - strand at 339.026 kb on - strand at 339.026 kb on - strand at 339.026 kb on - strand at 339.026 kb on - strand at 339.345 kb on + strand, within HGI48_RS01555 at 339.345 kb on + strand, within HGI48_RS01555 at 339.345 kb on + strand, within HGI48_RS01555 at 339.346 kb on - strand, within HGI48_RS01555 at 339.359 kb on + strand, within HGI48_RS01555 at 339.360 kb on - strand, within HGI48_RS01555
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 337,524 + -1.3 337,610 + +0.5 337,611 - -0.6 337,615 + +0.4 337,616 - +0.6 337,653 + +1.0 337,653 + +0.0 337,722 + -1.2 337,722 + +0.6 337,722 + +0.3 337,723 - -0.8 337,754 + -1.6 337,902 - HGI48_RS01545 0.21 -2.0 337,936 - HGI48_RS01545 0.26 -1.6 337,936 - -0.0 337,945 + HGI48_RS01545 0.27 -1.6 337,977 + HGI48_RS01545 0.32 -1.0 337,977 + -0.6 337,977 + HGI48_RS01545 0.32 -1.0 337,977 + HGI48_RS01545 0.32 -0.8 337,977 + HGI48_RS01545 0.32 +0.2 337,977 + HGI48_RS01545 0.32 -0.8 337,977 + HGI48_RS01545 0.32 -1.6 337,977 + HGI48_RS01545 0.32 -1.7 337,977 + HGI48_RS01545 0.32 -0.2 337,977 + HGI48_RS01545 0.32 -0.1 337,977 + HGI48_RS01545 0.32 -1.6 337,978 - HGI48_RS01545 0.32 +0.1 337,978 - HGI48_RS01545 0.32 +1.1 337,978 - HGI48_RS01545 0.32 -0.2 337,978 - HGI48_RS01545 0.32 -0.9 337,978 - HGI48_RS01545 0.32 -0.3 337,978 - HGI48_RS01545 0.32 -1.6 337,978 - HGI48_RS01545 0.32 -2.7 337,978 - -0.0 337,978 - HGI48_RS01545 0.32 -0.7 337,978 - +1.4 337,978 - +0.4 337,988 + HGI48_RS01545 0.34 +0.4 337,988 + HGI48_RS01545 0.34 +0.4 337,988 + HGI48_RS01545 0.34 -2.2 337,988 + HGI48_RS01545 0.34 +0.2 337,989 - HGI48_RS01545 0.34 -2.2 337,989 - HGI48_RS01545 0.34 -1.3 337,989 - HGI48_RS01545 0.34 -1.3 337,989 - HGI48_RS01545 0.34 +0.3 337,989 - HGI48_RS01545 0.34 -1.0 337,989 - HGI48_RS01545 0.34 -3.1 337,989 - HGI48_RS01545 0.34 +0.6 337,989 - HGI48_RS01545 0.34 -0.6 337,989 - HGI48_RS01545 0.34 -1.5 337,989 - HGI48_RS01545 0.34 -0.3 338,001 + HGI48_RS01545 0.36 -1.3 338,001 + HGI48_RS01545 0.36 +1.8 338,001 + HGI48_RS01545 0.36 -1.0 338,002 - HGI48_RS01545 0.36 -2.3 338,002 - HGI48_RS01545 0.36 -0.0 338,002 - HGI48_RS01545 0.36 +0.6 338,101 - +1.0 338,211 + HGI48_RS01545 0.69 +0.7 338,247 + HGI48_RS01545 0.74 +0.2 338,247 + HGI48_RS01545 0.74 -0.6 338,247 + HGI48_RS01545 0.74 -1.0 338,248 - HGI48_RS01545 0.74 -2.0 338,325 + HGI48_RS01545 0.86 -0.8 338,326 - HGI48_RS01545 0.87 -0.2 338,326 - +0.4 338,326 - HGI48_RS01545 0.87 -1.6 338,410 - -1.6 338,449 + -1.7 338,450 - -0.3 338,450 - -0.0 338,453 + -2.0 338,453 + -2.3 338,453 + -0.5 338,454 - +1.6 338,454 - -0.0 338,457 - +2.1 338,457 - -0.6 338,467 + HGI48_RS01550 0.10 +1.0 338,467 + HGI48_RS01550 0.10 -1.6 338,467 + HGI48_RS01550 0.10 +1.5 338,468 - HGI48_RS01550 0.10 -2.7 338,468 - HGI48_RS01550 0.10 -1.3 338,468 - HGI48_RS01550 0.10 +0.2 338,468 - HGI48_RS01550 0.10 -0.8 338,468 - HGI48_RS01550 0.10 +1.1 338,573 + HGI48_RS01550 0.32 -1.3 338,573 + HGI48_RS01550 0.32 -0.6 338,573 + HGI48_RS01550 0.32 -0.0 338,577 + HGI48_RS01550 0.32 -0.8 338,578 - HGI48_RS01550 0.33 -1.5 338,578 - HGI48_RS01550 0.33 -1.0 338,625 - HGI48_RS01550 0.42 -0.4 338,695 + HGI48_RS01550 0.56 -1.0 338,696 - HGI48_RS01550 0.56 -1.8 338,696 - HGI48_RS01550 0.56 +0.2 338,911 + -0.6 338,911 + -1.0 339,014 - +0.4 339,025 + -1.3 339,025 + -2.2 339,026 - +0.7 339,026 - -1.8 339,026 - +1.6 339,026 - -1.1 339,026 - -1.8 339,026 - -1.3 339,026 - -1.6 339,345 + HGI48_RS01555 0.23 -0.2 339,345 + HGI48_RS01555 0.23 -1.0 339,345 + HGI48_RS01555 0.23 -1.0 339,346 - HGI48_RS01555 0.24 -2.6 339,359 + HGI48_RS01555 0.24 -0.8 339,360 - HGI48_RS01555 0.25 -2.2
Or see this region's nucleotide sequence