Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01170 and HGI48_RS01175 are separated by 431 nucleotides HGI48_RS01175 and HGI48_RS01180 are separated by 105 nucleotides HGI48_RS01180 and HGI48_RS01185 are separated by 55 nucleotides
HGI48_RS01170: HGI48_RS01170 - phosphomethylpyrimidine synthase ThiC, at 261,682 to 263,553
_RS01170
HGI48_RS01175: HGI48_RS01175 - sigma D regulator, at 263,985 to 264,434
_RS01175
HGI48_RS01180: HGI48_RS01180 - NAD(+) diphosphatase, at 264,540 to 265,313
_RS01180
HGI48_RS01185: HGI48_RS01185 - uroporphyrinogen decarboxylase, at 265,369 to 266,442
_RS01185
Position (kb) 264
265
266 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 263.554 kb on - strand at 263.625 kb on + strand at 263.667 kb on + strand at 263.667 kb on + strand at 263.667 kb on + strand at 263.667 kb on + strand at 263.685 kb on + strand at 263.770 kb on + strand at 263.820 kb on + strand at 263.820 kb on + strand at 263.820 kb on + strand at 263.821 kb on - strand at 263.841 kb on + strand at 263.841 kb on + strand at 263.841 kb on + strand at 263.841 kb on + strand at 263.841 kb on + strand at 263.841 kb on + strand at 263.842 kb on - strand at 263.842 kb on - strand at 263.842 kb on - strand at 263.842 kb on - strand at 263.842 kb on - strand at 263.947 kb on + strand at 263.947 kb on + strand at 263.947 kb on + strand at 263.947 kb on + strand at 263.947 kb on + strand at 263.948 kb on - strand at 263.948 kb on - strand at 263.948 kb on - strand at 263.963 kb on + strand at 263.963 kb on + strand at 263.963 kb on + strand at 263.963 kb on + strand at 263.964 kb on - strand at 263.964 kb on - strand at 263.964 kb on - strand at 263.964 kb on - strand at 263.964 kb on - strand at 263.994 kb on + strand at 263.994 kb on + strand at 263.994 kb on + strand at 263.994 kb on + strand at 263.994 kb on + strand at 263.994 kb on + strand at 263.994 kb on + strand at 263.995 kb on - strand at 263.995 kb on - strand at 263.995 kb on - strand at 263.995 kb on - strand at 263.995 kb on - strand at 263.995 kb on - strand at 263.995 kb on - strand at 264.121 kb on + strand, within HGI48_RS01175 at 264.121 kb on + strand, within HGI48_RS01175 at 264.121 kb on + strand, within HGI48_RS01175 at 264.121 kb on + strand, within HGI48_RS01175 at 264.121 kb on + strand, within HGI48_RS01175 at 264.121 kb on + strand, within HGI48_RS01175 at 264.122 kb on - strand, within HGI48_RS01175 at 264.229 kb on + strand at 264.229 kb on + strand, within HGI48_RS01175 at 264.229 kb on + strand, within HGI48_RS01175 at 264.230 kb on - strand, within HGI48_RS01175 at 264.256 kb on + strand, within HGI48_RS01175 at 264.256 kb on + strand, within HGI48_RS01175 at 264.256 kb on + strand, within HGI48_RS01175 at 264.256 kb on + strand at 264.257 kb on - strand at 264.257 kb on - strand, within HGI48_RS01175 at 264.257 kb on - strand, within HGI48_RS01175 at 264.321 kb on - strand, within HGI48_RS01175 at 264.334 kb on + strand, within HGI48_RS01175 at 264.334 kb on + strand, within HGI48_RS01175 at 264.335 kb on - strand, within HGI48_RS01175 at 264.337 kb on + strand at 264.338 kb on - strand, within HGI48_RS01175 at 264.338 kb on - strand at 264.388 kb on + strand, within HGI48_RS01175 at 264.388 kb on + strand, within HGI48_RS01175 at 264.388 kb on + strand, within HGI48_RS01175 at 264.389 kb on - strand, within HGI48_RS01175 at 264.389 kb on - strand, within HGI48_RS01175 at 264.389 kb on - strand, within HGI48_RS01175 at 264.389 kb on - strand, within HGI48_RS01175 at 264.420 kb on + strand at 264.421 kb on - strand at 264.421 kb on - strand at 264.421 kb on - strand at 264.454 kb on + strand at 264.455 kb on - strand at 264.455 kb on - strand at 264.455 kb on - strand at 264.475 kb on + strand at 264.481 kb on + strand at 264.482 kb on - strand at 264.482 kb on - strand at 264.485 kb on + strand at 264.485 kb on + strand at 264.486 kb on - strand at 264.493 kb on + strand at 264.494 kb on - strand at 264.499 kb on + strand at 264.499 kb on + strand at 264.499 kb on + strand at 264.518 kb on + strand at 264.519 kb on - strand at 264.533 kb on - strand at 264.585 kb on + strand at 264.585 kb on + strand at 264.586 kb on - strand at 264.623 kb on - strand at 264.719 kb on + strand, within HGI48_RS01180 at 264.719 kb on + strand at 264.814 kb on - strand, within HGI48_RS01180 at 264.814 kb on - strand, within HGI48_RS01180 at 264.875 kb on - strand, within HGI48_RS01180 at 264.896 kb on + strand, within HGI48_RS01180 at 264.897 kb on - strand, within HGI48_RS01180 at 264.907 kb on - strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand at 265.000 kb on - strand, within HGI48_RS01180 at 265.000 kb on - strand, within HGI48_RS01180 at 265.000 kb on - strand at 265.000 kb on - strand, within HGI48_RS01180 at 265.000 kb on - strand, within HGI48_RS01180 at 265.000 kb on - strand, within HGI48_RS01180 at 265.080 kb on - strand, within HGI48_RS01180 at 265.101 kb on + strand, within HGI48_RS01180 at 265.101 kb on + strand, within HGI48_RS01180 at 265.101 kb on + strand, within HGI48_RS01180 at 265.102 kb on - strand, within HGI48_RS01180 at 265.102 kb on - strand, within HGI48_RS01180 at 265.102 kb on - strand, within HGI48_RS01180 at 265.102 kb on - strand, within HGI48_RS01180 at 265.161 kb on + strand, within HGI48_RS01180 at 265.221 kb on + strand, within HGI48_RS01180 at 265.222 kb on - strand, within HGI48_RS01180 at 265.286 kb on - strand at 265.288 kb on + strand at 265.288 kb on + strand at 265.288 kb on + strand at 265.289 kb on - strand at 265.289 kb on - strand at 265.289 kb on - strand at 265.355 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 263,554 - -0.3 263,625 + +1.2 263,667 + +0.5 263,667 + -0.1 263,667 + +1.0 263,667 + -0.6 263,685 + +0.3 263,770 + -0.4 263,820 + -0.6 263,820 + +1.4 263,820 + +0.4 263,821 - -0.3 263,841 + +1.0 263,841 + +1.0 263,841 + -0.1 263,841 + +0.5 263,841 + +1.5 263,841 + +0.3 263,842 - +0.5 263,842 - -1.6 263,842 - -0.3 263,842 - -0.3 263,842 - -1.6 263,947 + -0.5 263,947 + +0.1 263,947 + -1.0 263,947 + +0.0 263,947 + +0.8 263,948 - +0.7 263,948 - -1.0 263,948 - -0.8 263,963 + -0.2 263,963 + -0.6 263,963 + -1.6 263,963 + -0.3 263,964 - +0.7 263,964 - +1.3 263,964 - +0.1 263,964 - +0.2 263,964 - -0.5 263,994 + +0.1 263,994 + +1.0 263,994 + -0.4 263,994 + +0.8 263,994 + -1.8 263,994 + +1.0 263,994 + +1.0 263,995 - +0.7 263,995 - +0.8 263,995 - -1.3 263,995 - +1.0 263,995 - -0.2 263,995 - -1.1 263,995 - +0.0 264,121 + HGI48_RS01175 0.30 +0.2 264,121 + HGI48_RS01175 0.30 -0.6 264,121 + HGI48_RS01175 0.30 -0.5 264,121 + HGI48_RS01175 0.30 -0.8 264,121 + HGI48_RS01175 0.30 -1.3 264,121 + HGI48_RS01175 0.30 +1.7 264,122 - HGI48_RS01175 0.30 +0.8 264,229 + +2.3 264,229 + HGI48_RS01175 0.54 -0.3 264,229 + HGI48_RS01175 0.54 -0.6 264,230 - HGI48_RS01175 0.54 -2.2 264,256 + HGI48_RS01175 0.60 -0.6 264,256 + HGI48_RS01175 0.60 +0.8 264,256 + HGI48_RS01175 0.60 +1.0 264,256 + +1.0 264,257 - +1.0 264,257 - HGI48_RS01175 0.60 -1.0 264,257 - HGI48_RS01175 0.60 -0.4 264,321 - HGI48_RS01175 0.75 -0.8 264,334 + HGI48_RS01175 0.78 -1.2 264,334 + HGI48_RS01175 0.78 -1.1 264,335 - HGI48_RS01175 0.78 +0.1 264,337 + +3.0 264,338 - HGI48_RS01175 0.78 +0.0 264,338 - -0.6 264,388 + HGI48_RS01175 0.90 +0.8 264,388 + HGI48_RS01175 0.90 +0.6 264,388 + HGI48_RS01175 0.90 -1.3 264,389 - HGI48_RS01175 0.90 +0.5 264,389 - HGI48_RS01175 0.90 +2.2 264,389 - HGI48_RS01175 0.90 +1.4 264,389 - HGI48_RS01175 0.90 +1.0 264,420 + -1.1 264,421 - -1.0 264,421 - -0.8 264,421 - +0.6 264,454 + -0.1 264,455 - +1.6 264,455 - -1.0 264,455 - +1.5 264,475 + -0.6 264,481 + +1.7 264,482 - -0.2 264,482 - +1.8 264,485 + +2.2 264,485 + +0.3 264,486 - +0.1 264,493 + +0.4 264,494 - +0.0 264,499 + -0.2 264,499 + -0.6 264,499 + +0.0 264,518 + -1.8 264,519 - -0.3 264,533 - -1.3 264,585 + -0.2 264,585 + -0.4 264,586 - -1.3 264,623 - +2.8 264,719 + HGI48_RS01180 0.23 +0.0 264,719 + +1.8 264,814 - HGI48_RS01180 0.35 -0.2 264,814 - HGI48_RS01180 0.35 -0.2 264,875 - HGI48_RS01180 0.43 +0.0 264,896 + HGI48_RS01180 0.46 +0.2 264,897 - HGI48_RS01180 0.46 +0.0 264,907 - HGI48_RS01180 0.47 -0.6 264,999 + HGI48_RS01180 0.59 +0.2 264,999 + HGI48_RS01180 0.59 -1.1 264,999 + HGI48_RS01180 0.59 -0.6 264,999 + HGI48_RS01180 0.59 +0.0 264,999 + HGI48_RS01180 0.59 +0.1 264,999 + HGI48_RS01180 0.59 -0.4 264,999 + -0.6 264,999 + HGI48_RS01180 0.59 -1.0 264,999 + +0.0 265,000 - HGI48_RS01180 0.59 -0.8 265,000 - HGI48_RS01180 0.59 -1.3 265,000 - -0.6 265,000 - HGI48_RS01180 0.59 +0.2 265,000 - HGI48_RS01180 0.59 +0.7 265,000 - HGI48_RS01180 0.59 +0.3 265,080 - HGI48_RS01180 0.70 +0.5 265,101 + HGI48_RS01180 0.72 -1.2 265,101 + HGI48_RS01180 0.72 +1.0 265,101 + HGI48_RS01180 0.72 -0.8 265,102 - HGI48_RS01180 0.73 +0.8 265,102 - HGI48_RS01180 0.73 +0.1 265,102 - HGI48_RS01180 0.73 +0.2 265,102 - HGI48_RS01180 0.73 -1.0 265,161 + HGI48_RS01180 0.80 -1.9 265,221 + HGI48_RS01180 0.88 +0.3 265,222 - HGI48_RS01180 0.88 -0.3 265,286 - +0.5 265,288 + +0.8 265,288 + +0.3 265,288 + +0.5 265,289 - -1.0 265,289 - -0.6 265,289 - +1.0 265,355 - -1.6
Or see this region's nucleotide sequence