Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01105 and HGI48_RS01110 are separated by 224 nucleotides HGI48_RS01110 and HGI48_RS01115 are separated by 159 nucleotides HGI48_RS01115 and HGI48_RS01120 are separated by 11 nucleotides HGI48_RS01120 and HGI48_RS01125 overlap by 8 nucleotides
HGI48_RS01105: HGI48_RS01105 - DUF1127 domain-containing protein, at 251,676 to 251,858
_RS01105
HGI48_RS01110: HGI48_RS01110 - DUF1127 domain-containing protein, at 252,083 to 252,268
_RS01110
HGI48_RS01115: HGI48_RS01115 - PLP-dependent aminotransferase family protein, at 252,428 to 253,864
_RS01115
HGI48_RS01120: HGI48_RS01120 - molybdopterin-dependent oxidoreductase, at 253,876 to 254,643
_RS01120
HGI48_RS01125: HGI48_RS01125 - cytochrome b/b6 domain-containing protein, at 254,636 to 255,238
_RS01125
Position (kb)
252
253
254 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 251.478 kb on + strand at 251.478 kb on + strand at 251.479 kb on - strand at 251.498 kb on - strand at 251.514 kb on + strand at 251.514 kb on + strand at 251.514 kb on + strand at 251.515 kb on - strand at 251.515 kb on - strand at 251.546 kb on + strand at 251.547 kb on - strand at 251.594 kb on + strand at 251.594 kb on + strand at 251.595 kb on - strand at 251.595 kb on - strand at 251.595 kb on - strand at 251.595 kb on - strand at 251.612 kb on + strand at 251.648 kb on + strand at 251.648 kb on + strand at 251.648 kb on + strand at 251.656 kb on - strand at 251.739 kb on - strand, within HGI48_RS01105 at 251.831 kb on + strand, within HGI48_RS01105 at 251.832 kb on - strand, within HGI48_RS01105 at 251.832 kb on - strand, within HGI48_RS01105 at 251.888 kb on + strand at 251.889 kb on - strand at 251.944 kb on + strand at 251.944 kb on + strand at 251.945 kb on - strand at 251.945 kb on - strand at 251.945 kb on - strand at 251.945 kb on - strand at 251.945 kb on - strand at 251.994 kb on - strand at 252.023 kb on - strand at 252.035 kb on + strand at 252.262 kb on + strand at 252.262 kb on + strand at 252.262 kb on + strand at 252.263 kb on - strand at 252.263 kb on - strand at 252.271 kb on + strand at 252.271 kb on + strand at 252.272 kb on - strand at 252.272 kb on - strand at 252.272 kb on - strand at 252.356 kb on + strand at 252.357 kb on - strand at 252.367 kb on + strand at 252.406 kb on - strand at 252.456 kb on + strand at 252.456 kb on + strand at 252.457 kb on - strand at 252.457 kb on - strand at 252.555 kb on + strand at 252.556 kb on - strand at 252.556 kb on - strand at 252.566 kb on + strand at 252.566 kb on + strand at 252.567 kb on - strand at 252.569 kb on + strand at 252.569 kb on + strand at 252.569 kb on + strand at 252.570 kb on - strand at 252.570 kb on - strand at 252.570 kb on - strand at 252.720 kb on + strand, within HGI48_RS01115 at 252.721 kb on - strand, within HGI48_RS01115 at 252.721 kb on - strand, within HGI48_RS01115 at 252.790 kb on + strand, within HGI48_RS01115 at 252.791 kb on - strand, within HGI48_RS01115 at 252.851 kb on + strand, within HGI48_RS01115 at 252.851 kb on + strand, within HGI48_RS01115 at 252.851 kb on + strand, within HGI48_RS01115 at 253.163 kb on - strand, within HGI48_RS01115 at 253.206 kb on + strand, within HGI48_RS01115 at 253.206 kb on + strand, within HGI48_RS01115 at 253.244 kb on + strand, within HGI48_RS01115 at 253.245 kb on - strand, within HGI48_RS01115 at 253.255 kb on + strand, within HGI48_RS01115 at 253.274 kb on + strand, within HGI48_RS01115 at 253.275 kb on - strand, within HGI48_RS01115 at 253.275 kb on - strand, within HGI48_RS01115 at 253.275 kb on - strand, within HGI48_RS01115 at 253.290 kb on - strand, within HGI48_RS01115 at 253.351 kb on + strand, within HGI48_RS01115 at 253.351 kb on + strand, within HGI48_RS01115 at 253.351 kb on + strand, within HGI48_RS01115 at 253.352 kb on - strand, within HGI48_RS01115 at 253.352 kb on - strand, within HGI48_RS01115 at 253.439 kb on + strand, within HGI48_RS01115 at 253.439 kb on + strand, within HGI48_RS01115 at 253.439 kb on + strand, within HGI48_RS01115 at 253.439 kb on + strand at 253.439 kb on + strand, within HGI48_RS01115 at 253.439 kb on + strand, within HGI48_RS01115 at 253.440 kb on - strand, within HGI48_RS01115 at 253.440 kb on - strand, within HGI48_RS01115 at 253.440 kb on - strand, within HGI48_RS01115 at 253.509 kb on - strand, within HGI48_RS01115 at 253.541 kb on + strand, within HGI48_RS01115 at 253.541 kb on + strand, within HGI48_RS01115 at 253.542 kb on - strand, within HGI48_RS01115 at 253.549 kb on + strand, within HGI48_RS01115 at 253.549 kb on + strand, within HGI48_RS01115 at 253.549 kb on + strand, within HGI48_RS01115 at 253.549 kb on + strand, within HGI48_RS01115 at 253.550 kb on - strand, within HGI48_RS01115 at 253.597 kb on + strand, within HGI48_RS01115 at 253.598 kb on - strand, within HGI48_RS01115 at 253.633 kb on - strand, within HGI48_RS01115 at 253.693 kb on + strand, within HGI48_RS01115 at 253.693 kb on + strand at 253.693 kb on + strand, within HGI48_RS01115 at 253.702 kb on + strand, within HGI48_RS01115 at 253.702 kb on + strand, within HGI48_RS01115 at 253.702 kb on + strand, within HGI48_RS01115 at 253.703 kb on - strand, within HGI48_RS01115 at 253.739 kb on + strand at 253.739 kb on + strand at 253.756 kb on + strand at 253.756 kb on + strand at 253.788 kb on + strand at 253.789 kb on - strand at 253.896 kb on + strand at 253.896 kb on + strand at 253.896 kb on + strand at 253.896 kb on + strand at 253.896 kb on + strand at 253.896 kb on + strand at 253.896 kb on + strand at 253.896 kb on + strand at 253.897 kb on - strand at 253.897 kb on - strand at 253.898 kb on + strand at 253.898 kb on + strand at 253.898 kb on + strand at 253.899 kb on - strand at 253.899 kb on - strand at 253.899 kb on - strand at 253.899 kb on - strand at 253.899 kb on - strand at 253.899 kb on - strand at 253.899 kb on - strand at 253.899 kb on - strand at 253.899 kb on - strand at 253.956 kb on + strand, within HGI48_RS01120 at 254.099 kb on + strand, within HGI48_RS01120 at 254.099 kb on + strand, within HGI48_RS01120 at 254.099 kb on + strand, within HGI48_RS01120 at 254.100 kb on - strand, within HGI48_RS01120 at 254.100 kb on - strand at 254.100 kb on - strand, within HGI48_RS01120 at 254.100 kb on - strand, within HGI48_RS01120 at 254.100 kb on - strand, within HGI48_RS01120 at 254.100 kb on - strand, within HGI48_RS01120 at 254.118 kb on + strand, within HGI48_RS01120 at 254.118 kb on + strand, within HGI48_RS01120 at 254.118 kb on + strand, within HGI48_RS01120 at 254.129 kb on + strand, within HGI48_RS01120 at 254.155 kb on - strand, within HGI48_RS01120 at 254.173 kb on + strand at 254.217 kb on + strand, within HGI48_RS01120 at 254.217 kb on + strand, within HGI48_RS01120 at 254.217 kb on + strand, within HGI48_RS01120 at 254.223 kb on + strand, within HGI48_RS01120 at 254.223 kb on + strand, within HGI48_RS01120 at 254.223 kb on + strand, within HGI48_RS01120 at 254.223 kb on + strand, within HGI48_RS01120 at 254.223 kb on + strand at 254.223 kb on + strand, within HGI48_RS01120 at 254.223 kb on + strand, within HGI48_RS01120 at 254.224 kb on - strand, within HGI48_RS01120 at 254.224 kb on - strand, within HGI48_RS01120 at 254.224 kb on - strand, within HGI48_RS01120 at 254.224 kb on - strand, within HGI48_RS01120 at 254.260 kb on + strand, within HGI48_RS01120 at 254.261 kb on - strand, within HGI48_RS01120 at 254.261 kb on - strand, within HGI48_RS01120 at 254.261 kb on - strand, within HGI48_RS01120 at 254.319 kb on - strand, within HGI48_RS01120 at 254.348 kb on + strand, within HGI48_RS01120 at 254.349 kb on - strand, within HGI48_RS01120 at 254.358 kb on - strand, within HGI48_RS01120 at 254.402 kb on + strand, within HGI48_RS01120 at 254.403 kb on - strand, within HGI48_RS01120 at 254.505 kb on + strand, within HGI48_RS01120 at 254.505 kb on + strand, within HGI48_RS01120 at 254.505 kb on + strand, within HGI48_RS01120 at 254.505 kb on + strand, within HGI48_RS01120 at 254.505 kb on + strand, within HGI48_RS01120 at 254.505 kb on + strand, within HGI48_RS01120 at 254.505 kb on + strand, within HGI48_RS01120 at 254.506 kb on - strand, within HGI48_RS01120 at 254.506 kb on - strand, within HGI48_RS01120 at 254.506 kb on - strand, within HGI48_RS01120 at 254.506 kb on - strand, within HGI48_RS01120 at 254.508 kb on + strand, within HGI48_RS01120 at 254.508 kb on + strand, within HGI48_RS01120 at 254.508 kb on + strand, within HGI48_RS01120 at 254.508 kb on + strand, within HGI48_RS01120 at 254.508 kb on + strand, within HGI48_RS01120 at 254.508 kb on + strand, within HGI48_RS01120 at 254.509 kb on - strand, within HGI48_RS01120 at 254.509 kb on - strand, within HGI48_RS01120 at 254.608 kb on + strand at 254.635 kb on - strand at 254.669 kb on + strand at 254.674 kb on + strand at 254.708 kb on + strand, within HGI48_RS01125 at 254.708 kb on + strand at 254.708 kb on + strand, within HGI48_RS01125 at 254.752 kb on - strand, within HGI48_RS01125 at 254.752 kb on - strand at 254.752 kb on - strand, within HGI48_RS01125 at 254.782 kb on + strand, within HGI48_RS01125 at 254.783 kb on - strand, within HGI48_RS01125 at 254.783 kb on - strand at 254.783 kb on - strand, within HGI48_RS01125 at 254.841 kb on - strand, within HGI48_RS01125
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 251,478 + +1.0 251,478 + +0.8 251,479 - +2.5 251,498 - -0.2 251,514 + +0.5 251,514 + -1.8 251,514 + +0.3 251,515 - -0.3 251,515 - -0.2 251,546 + -0.2 251,547 - +0.5 251,594 + +1.4 251,594 + +1.3 251,595 - -2.0 251,595 - +0.6 251,595 - +0.6 251,595 - -0.7 251,612 + -0.9 251,648 + -1.3 251,648 + -2.0 251,648 + -1.0 251,656 - +0.4 251,739 - HGI48_RS01105 0.34 +0.6 251,831 + HGI48_RS01105 0.85 -0.9 251,832 - HGI48_RS01105 0.85 -1.8 251,832 - HGI48_RS01105 0.85 +0.0 251,888 + +0.2 251,889 - +1.4 251,944 + -0.5 251,944 + +2.7 251,945 - -0.6 251,945 - -0.2 251,945 - +0.6 251,945 - +0.0 251,945 - -0.3 251,994 - +0.3 252,023 - +1.8 252,035 + +0.2 252,262 + +0.6 252,262 + +0.1 252,262 + -0.3 252,263 - -0.5 252,263 - +0.7 252,271 + -0.2 252,271 + +1.1 252,272 - -0.5 252,272 - +0.0 252,272 - +0.4 252,356 + -0.8 252,357 - +0.2 252,367 + +0.4 252,406 - +1.6 252,456 + +0.0 252,456 + +0.7 252,457 - +0.3 252,457 - -1.7 252,555 + -1.0 252,556 - -1.0 252,556 - +0.0 252,566 + +0.6 252,566 + +1.5 252,567 - -0.3 252,569 + +0.3 252,569 + +2.2 252,569 + -1.8 252,570 - -0.6 252,570 - -1.2 252,570 - +0.0 252,720 + HGI48_RS01115 0.20 -0.4 252,721 - HGI48_RS01115 0.20 +2.0 252,721 - HGI48_RS01115 0.20 +1.0 252,790 + HGI48_RS01115 0.25 -0.8 252,791 - HGI48_RS01115 0.25 -0.4 252,851 + HGI48_RS01115 0.29 -0.8 252,851 + HGI48_RS01115 0.29 +0.0 252,851 + HGI48_RS01115 0.29 +0.0 253,163 - HGI48_RS01115 0.51 -0.3 253,206 + HGI48_RS01115 0.54 -0.2 253,206 + HGI48_RS01115 0.54 +0.0 253,244 + HGI48_RS01115 0.57 -1.6 253,245 - HGI48_RS01115 0.57 -0.8 253,255 + HGI48_RS01115 0.58 -0.3 253,274 + HGI48_RS01115 0.59 -0.4 253,275 - HGI48_RS01115 0.59 -0.4 253,275 - HGI48_RS01115 0.59 -1.0 253,275 - HGI48_RS01115 0.59 -1.0 253,290 - HGI48_RS01115 0.60 -1.6 253,351 + HGI48_RS01115 0.64 +2.0 253,351 + HGI48_RS01115 0.64 +0.8 253,351 + HGI48_RS01115 0.64 +1.3 253,352 - HGI48_RS01115 0.64 +0.6 253,352 - HGI48_RS01115 0.64 -1.8 253,439 + HGI48_RS01115 0.70 +0.0 253,439 + HGI48_RS01115 0.70 -0.6 253,439 + HGI48_RS01115 0.70 -0.6 253,439 + +1.6 253,439 + HGI48_RS01115 0.70 +0.4 253,439 + HGI48_RS01115 0.70 +1.7 253,440 - HGI48_RS01115 0.70 +0.2 253,440 - HGI48_RS01115 0.70 -2.3 253,440 - HGI48_RS01115 0.70 +1.3 253,509 - HGI48_RS01115 0.75 -1.8 253,541 + HGI48_RS01115 0.77 -0.7 253,541 + HGI48_RS01115 0.77 +0.2 253,542 - HGI48_RS01115 0.78 -2.0 253,549 + HGI48_RS01115 0.78 -2.2 253,549 + HGI48_RS01115 0.78 -1.9 253,549 + HGI48_RS01115 0.78 -1.0 253,549 + HGI48_RS01115 0.78 +0.7 253,550 - HGI48_RS01115 0.78 +0.0 253,597 + HGI48_RS01115 0.81 -1.8 253,598 - HGI48_RS01115 0.81 -1.4 253,633 - HGI48_RS01115 0.84 +1.2 253,693 + HGI48_RS01115 0.88 +0.0 253,693 + -0.6 253,693 + HGI48_RS01115 0.88 -1.8 253,702 + HGI48_RS01115 0.89 +0.7 253,702 + HGI48_RS01115 0.89 -0.6 253,702 + HGI48_RS01115 0.89 -0.8 253,703 - HGI48_RS01115 0.89 -0.6 253,739 + -0.7 253,739 + +0.6 253,756 + -0.2 253,756 + +0.5 253,788 + -1.0 253,789 - -1.1 253,896 + +0.4 253,896 + -2.6 253,896 + -0.2 253,896 + +0.0 253,896 + -1.8 253,896 + +0.9 253,896 + -0.6 253,896 + -1.0 253,897 - +0.1 253,897 - -1.1 253,898 + -0.8 253,898 + +0.0 253,898 + -0.3 253,899 - -0.4 253,899 - -1.8 253,899 - -0.5 253,899 - +0.4 253,899 - -2.2 253,899 - -1.0 253,899 - +0.6 253,899 - +1.0 253,899 - +0.8 253,956 + HGI48_RS01120 0.10 +0.2 254,099 + HGI48_RS01120 0.29 -1.0 254,099 + HGI48_RS01120 0.29 -2.0 254,099 + HGI48_RS01120 0.29 +0.0 254,100 - HGI48_RS01120 0.29 -0.4 254,100 - +1.0 254,100 - HGI48_RS01120 0.29 +0.3 254,100 - HGI48_RS01120 0.29 -1.0 254,100 - HGI48_RS01120 0.29 -1.3 254,100 - HGI48_RS01120 0.29 -0.7 254,118 + HGI48_RS01120 0.32 +0.5 254,118 + HGI48_RS01120 0.32 +0.4 254,118 + HGI48_RS01120 0.32 +1.5 254,129 + HGI48_RS01120 0.33 -0.2 254,155 - HGI48_RS01120 0.36 +0.5 254,173 + +2.6 254,217 + HGI48_RS01120 0.44 -0.7 254,217 + HGI48_RS01120 0.44 -1.0 254,217 + HGI48_RS01120 0.44 +0.4 254,223 + HGI48_RS01120 0.45 +0.8 254,223 + HGI48_RS01120 0.45 -0.2 254,223 + HGI48_RS01120 0.45 -1.0 254,223 + HGI48_RS01120 0.45 +1.0 254,223 + +2.0 254,223 + HGI48_RS01120 0.45 -1.0 254,223 + HGI48_RS01120 0.45 -0.2 254,224 - HGI48_RS01120 0.45 +0.2 254,224 - HGI48_RS01120 0.45 +0.8 254,224 - HGI48_RS01120 0.45 +0.8 254,224 - HGI48_RS01120 0.45 -0.4 254,260 + HGI48_RS01120 0.50 +0.8 254,261 - HGI48_RS01120 0.50 +2.4 254,261 - HGI48_RS01120 0.50 -1.8 254,261 - HGI48_RS01120 0.50 +0.7 254,319 - HGI48_RS01120 0.58 +1.0 254,348 + HGI48_RS01120 0.61 -0.7 254,349 - HGI48_RS01120 0.62 +0.3 254,358 - HGI48_RS01120 0.63 +0.3 254,402 + HGI48_RS01120 0.68 -1.3 254,403 - HGI48_RS01120 0.69 +1.6 254,505 + HGI48_RS01120 0.82 +1.0 254,505 + HGI48_RS01120 0.82 +0.6 254,505 + HGI48_RS01120 0.82 +0.1 254,505 + HGI48_RS01120 0.82 -0.9 254,505 + HGI48_RS01120 0.82 -0.9 254,505 + HGI48_RS01120 0.82 +0.4 254,505 + HGI48_RS01120 0.82 -0.3 254,506 - HGI48_RS01120 0.82 -1.0 254,506 - HGI48_RS01120 0.82 +0.2 254,506 - HGI48_RS01120 0.82 -0.8 254,506 - HGI48_RS01120 0.82 -1.7 254,508 + HGI48_RS01120 0.82 -0.9 254,508 + HGI48_RS01120 0.82 -1.3 254,508 + HGI48_RS01120 0.82 -1.4 254,508 + HGI48_RS01120 0.82 +1.6 254,508 + HGI48_RS01120 0.82 +0.0 254,508 + HGI48_RS01120 0.82 -1.3 254,509 - HGI48_RS01120 0.82 -0.6 254,509 - HGI48_RS01120 0.82 +1.0 254,608 + -1.9 254,635 - +1.0 254,669 + -0.8 254,674 + +0.5 254,708 + HGI48_RS01125 0.12 +0.3 254,708 + -0.6 254,708 + HGI48_RS01125 0.12 +0.0 254,752 - HGI48_RS01125 0.19 +1.9 254,752 - +2.0 254,752 - HGI48_RS01125 0.19 +0.0 254,782 + HGI48_RS01125 0.24 +0.4 254,783 - HGI48_RS01125 0.24 -0.8 254,783 - +1.0 254,783 - HGI48_RS01125 0.24 +0.8 254,841 - HGI48_RS01125 0.34 +0.3
Or see this region's nucleotide sequence