Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS00780 and HGI48_RS00785 are separated by 226 nucleotides HGI48_RS00785 and HGI48_RS00790 are separated by 99 nucleotides HGI48_RS00790 and HGI48_RS00795 overlap by 1 nucleotides
HGI48_RS00780: HGI48_RS00780 - murein hydrolase activator EnvC, at 178,465 to 179,739
_RS00780
HGI48_RS00785: HGI48_RS00785 - rhodanese-like domain-containing protein, at 179,966 to 180,397
_RS00785
HGI48_RS00790: HGI48_RS00790 - protein-export chaperone SecB, at 180,497 to 180,967
_RS00790
HGI48_RS00795: HGI48_RS00795 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 180,967 to 181,986
_RS00795
Position (kb) 179
180
181 Strain fitness (log2 ratio) -2
-1
0
1 at 179.264 kb on + strand, within HGI48_RS00780 at 179.686 kb on + strand at 179.750 kb on + strand at 179.751 kb on - strand at 179.838 kb on + strand at 179.858 kb on + strand at 179.859 kb on - strand at 179.881 kb on - strand at 179.889 kb on + strand at 179.890 kb on - strand at 179.905 kb on + strand at 179.905 kb on + strand at 179.905 kb on + strand at 179.905 kb on + strand at 179.905 kb on + strand at 179.906 kb on - strand at 179.909 kb on + strand at 179.909 kb on + strand at 179.909 kb on + strand at 179.909 kb on + strand at 179.909 kb on + strand at 179.910 kb on - strand at 179.910 kb on - strand at 179.910 kb on - strand at 179.910 kb on - strand at 179.910 kb on - strand at 179.910 kb on - strand at 179.910 kb on - strand at 179.910 kb on - strand at 179.910 kb on - strand at 179.911 kb on + strand at 179.911 kb on + strand at 179.911 kb on + strand at 179.912 kb on - strand at 179.912 kb on - strand at 179.926 kb on + strand at 179.926 kb on + strand at 179.977 kb on + strand at 179.977 kb on + strand at 179.977 kb on + strand at 180.050 kb on - strand, within HGI48_RS00785 at 180.112 kb on + strand, within HGI48_RS00785 at 180.115 kb on + strand, within HGI48_RS00785 at 180.172 kb on + strand, within HGI48_RS00785 at 180.392 kb on - strand at 180.395 kb on + strand at 180.396 kb on - strand at 180.432 kb on + strand at 180.490 kb on - strand at 180.546 kb on - strand, within HGI48_RS00790 at 180.556 kb on + strand, within HGI48_RS00790 at 180.914 kb on + strand, within HGI48_RS00790 at 180.914 kb on + strand, within HGI48_RS00790 at 181.150 kb on - strand, within HGI48_RS00795 at 181.356 kb on + strand, within HGI48_RS00795
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 179,264 + HGI48_RS00780 0.63 -0.5 179,686 + -1.0 179,750 + +1.4 179,751 - +0.7 179,838 + -1.8 179,858 + +0.0 179,859 - -1.0 179,881 - +0.6 179,889 + +1.0 179,890 - -0.3 179,905 + -1.0 179,905 + -2.2 179,905 + +0.0 179,905 + -0.4 179,905 + -0.2 179,906 - +0.0 179,909 + +0.3 179,909 + -1.0 179,909 + +0.2 179,909 + -1.2 179,909 + -1.8 179,910 - -1.9 179,910 - -1.7 179,910 - -1.0 179,910 - +1.0 179,910 - -2.4 179,910 - +1.2 179,910 - +1.2 179,910 - +1.6 179,910 - -0.2 179,911 + -1.6 179,911 + -0.2 179,911 + +0.4 179,912 - -0.9 179,912 - +0.7 179,926 + -0.2 179,926 + -1.6 179,977 + -1.3 179,977 + +1.0 179,977 + +0.0 180,050 - HGI48_RS00785 0.19 -1.3 180,112 + HGI48_RS00785 0.34 -0.7 180,115 + HGI48_RS00785 0.34 -1.4 180,172 + HGI48_RS00785 0.48 +0.5 180,392 - -2.1 180,395 + -1.2 180,396 - +1.3 180,432 + +1.0 180,490 - -1.0 180,546 - HGI48_RS00790 0.10 -0.2 180,556 + HGI48_RS00790 0.13 -0.3 180,914 + HGI48_RS00790 0.89 -0.0 180,914 + HGI48_RS00790 0.89 +0.6 181,150 - HGI48_RS00795 0.18 -0.8 181,356 + HGI48_RS00795 0.38 -1.3
Or see this region's nucleotide sequence