Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS17305 and HGI48_RS17310 are separated by 148 nucleotides HGI48_RS17310 and HGI48_RS17315 are separated by 98 nucleotides HGI48_RS17315 and HGI48_RS17320 are separated by 40 nucleotides HGI48_RS17320 and HGI48_RS17325 are separated by 372 nucleotides
HGI48_RS17305: HGI48_RS17305 - hypothetical protein, at 3,875,286 to 3,875,561
_RS17305
HGI48_RS17310: HGI48_RS17310 - 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB, at 3,875,710 to 3,876,444
_RS17310
HGI48_RS17315: HGI48_RS17315 - ribonuclease R, at 3,876,543 to 3,879,020
_RS17315
HGI48_RS17320: HGI48_RS17320 - nitric oxide-sensing transcriptional repressor NsrR, at 3,879,061 to 3,879,486
_RS17320
HGI48_RS17325: HGI48_RS17325 - Tar ligand binding domain-containing protein, at 3,879,859 to 3,881,445
_RS17325
Position (kb)
3876
3877
3878
3879
3880 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3875.559 kb on + strand at 3875.559 kb on + strand at 3875.560 kb on - strand at 3875.586 kb on + strand at 3875.586 kb on + strand at 3875.586 kb on + strand at 3875.587 kb on - strand at 3875.601 kb on + strand at 3875.601 kb on + strand at 3875.601 kb on + strand at 3875.602 kb on - strand at 3875.602 kb on - strand at 3875.602 kb on - strand at 3875.602 kb on - strand at 3875.602 kb on - strand at 3875.602 kb on - strand at 3875.602 kb on - strand at 3875.602 kb on - strand at 3875.616 kb on + strand at 3875.616 kb on + strand at 3875.616 kb on + strand at 3875.616 kb on + strand at 3875.616 kb on + strand at 3875.617 kb on - strand at 3875.617 kb on - strand at 3875.617 kb on - strand at 3875.631 kb on + strand at 3875.631 kb on + strand at 3875.632 kb on - strand at 3875.632 kb on - strand at 3875.666 kb on + strand at 3875.667 kb on - strand at 3876.190 kb on + strand at 3876.428 kb on + strand at 3876.432 kb on + strand at 3876.446 kb on - strand at 3876.446 kb on - strand at 3876.449 kb on - strand at 3876.455 kb on + strand at 3876.455 kb on + strand at 3876.456 kb on - strand at 3876.461 kb on + strand at 3876.461 kb on + strand at 3876.461 kb on + strand at 3876.461 kb on + strand at 3876.461 kb on + strand at 3876.461 kb on + strand at 3876.461 kb on + strand at 3876.462 kb on - strand at 3876.462 kb on - strand at 3876.462 kb on - strand at 3876.462 kb on - strand at 3876.474 kb on + strand at 3876.474 kb on + strand at 3876.475 kb on - strand at 3876.475 kb on - strand at 3876.824 kb on + strand, within HGI48_RS17315 at 3876.875 kb on + strand, within HGI48_RS17315 at 3876.875 kb on + strand, within HGI48_RS17315 at 3876.875 kb on + strand, within HGI48_RS17315 at 3876.979 kb on + strand, within HGI48_RS17315 at 3876.985 kb on + strand, within HGI48_RS17315 at 3876.985 kb on + strand, within HGI48_RS17315 at 3876.985 kb on + strand, within HGI48_RS17315 at 3876.986 kb on - strand, within HGI48_RS17315 at 3876.986 kb on - strand, within HGI48_RS17315 at 3876.988 kb on + strand, within HGI48_RS17315 at 3876.988 kb on + strand, within HGI48_RS17315 at 3876.988 kb on + strand at 3877.079 kb on + strand, within HGI48_RS17315 at 3877.079 kb on + strand, within HGI48_RS17315 at 3877.079 kb on + strand, within HGI48_RS17315 at 3877.079 kb on + strand, within HGI48_RS17315 at 3877.079 kb on + strand, within HGI48_RS17315 at 3877.116 kb on - strand, within HGI48_RS17315 at 3877.116 kb on - strand, within HGI48_RS17315 at 3877.140 kb on + strand, within HGI48_RS17315 at 3877.270 kb on + strand, within HGI48_RS17315 at 3877.271 kb on - strand, within HGI48_RS17315 at 3877.271 kb on - strand at 3877.271 kb on - strand, within HGI48_RS17315 at 3877.378 kb on + strand at 3877.378 kb on + strand, within HGI48_RS17315 at 3877.379 kb on - strand at 3877.465 kb on + strand, within HGI48_RS17315 at 3877.652 kb on + strand, within HGI48_RS17315 at 3877.652 kb on + strand, within HGI48_RS17315 at 3877.780 kb on + strand, within HGI48_RS17315 at 3877.780 kb on + strand, within HGI48_RS17315 at 3877.780 kb on + strand at 3877.780 kb on + strand, within HGI48_RS17315 at 3877.780 kb on + strand, within HGI48_RS17315 at 3877.780 kb on + strand, within HGI48_RS17315 at 3877.781 kb on - strand, within HGI48_RS17315 at 3877.781 kb on - strand, within HGI48_RS17315 at 3877.781 kb on - strand, within HGI48_RS17315 at 3877.781 kb on - strand, within HGI48_RS17315 at 3877.781 kb on - strand, within HGI48_RS17315 at 3877.781 kb on - strand, within HGI48_RS17315 at 3877.873 kb on + strand, within HGI48_RS17315 at 3877.873 kb on + strand, within HGI48_RS17315 at 3877.873 kb on + strand, within HGI48_RS17315 at 3877.873 kb on + strand, within HGI48_RS17315 at 3877.874 kb on - strand, within HGI48_RS17315 at 3877.874 kb on - strand, within HGI48_RS17315 at 3877.874 kb on - strand, within HGI48_RS17315 at 3877.909 kb on + strand, within HGI48_RS17315 at 3877.909 kb on + strand, within HGI48_RS17315 at 3877.909 kb on + strand, within HGI48_RS17315 at 3877.910 kb on - strand, within HGI48_RS17315 at 3877.910 kb on - strand at 3877.916 kb on + strand, within HGI48_RS17315 at 3877.916 kb on + strand, within HGI48_RS17315 at 3877.917 kb on - strand, within HGI48_RS17315 at 3877.965 kb on - strand, within HGI48_RS17315 at 3877.965 kb on - strand, within HGI48_RS17315 at 3877.965 kb on - strand at 3877.965 kb on - strand, within HGI48_RS17315 at 3878.050 kb on + strand, within HGI48_RS17315 at 3878.050 kb on + strand at 3878.050 kb on + strand, within HGI48_RS17315 at 3878.050 kb on + strand, within HGI48_RS17315 at 3878.050 kb on + strand, within HGI48_RS17315 at 3878.107 kb on + strand, within HGI48_RS17315 at 3878.107 kb on + strand, within HGI48_RS17315 at 3878.107 kb on + strand, within HGI48_RS17315 at 3878.108 kb on - strand, within HGI48_RS17315 at 3878.108 kb on - strand, within HGI48_RS17315 at 3878.110 kb on + strand, within HGI48_RS17315 at 3878.110 kb on + strand, within HGI48_RS17315 at 3878.110 kb on + strand, within HGI48_RS17315 at 3878.110 kb on + strand at 3878.110 kb on + strand, within HGI48_RS17315 at 3878.110 kb on + strand, within HGI48_RS17315 at 3878.110 kb on + strand, within HGI48_RS17315 at 3878.111 kb on - strand, within HGI48_RS17315 at 3878.170 kb on + strand at 3878.170 kb on + strand at 3878.333 kb on + strand, within HGI48_RS17315 at 3878.391 kb on + strand, within HGI48_RS17315 at 3878.391 kb on + strand, within HGI48_RS17315 at 3878.565 kb on + strand, within HGI48_RS17315 at 3878.695 kb on + strand, within HGI48_RS17315 at 3878.696 kb on - strand, within HGI48_RS17315 at 3878.795 kb on - strand at 3878.953 kb on + strand at 3878.980 kb on + strand at 3879.062 kb on + strand at 3879.062 kb on + strand at 3879.062 kb on + strand at 3879.076 kb on + strand at 3879.077 kb on - strand at 3879.077 kb on - strand at 3879.077 kb on - strand at 3879.077 kb on - strand at 3879.081 kb on + strand at 3879.082 kb on - strand at 3879.082 kb on - strand at 3879.123 kb on - strand, within HGI48_RS17320 at 3879.166 kb on + strand, within HGI48_RS17320 at 3879.193 kb on - strand, within HGI48_RS17320 at 3879.195 kb on + strand, within HGI48_RS17320 at 3879.195 kb on + strand, within HGI48_RS17320 at 3879.196 kb on - strand, within HGI48_RS17320 at 3879.196 kb on - strand at 3879.364 kb on - strand, within HGI48_RS17320 at 3879.438 kb on + strand, within HGI48_RS17320 at 3879.438 kb on + strand, within HGI48_RS17320 at 3879.440 kb on + strand, within HGI48_RS17320 at 3879.440 kb on + strand at 3879.440 kb on + strand, within HGI48_RS17320 at 3879.441 kb on - strand, within HGI48_RS17320 at 3879.461 kb on + strand at 3879.462 kb on - strand at 3879.479 kb on - strand at 3879.501 kb on - strand at 3879.612 kb on - strand at 3879.640 kb on - strand at 3879.643 kb on + strand at 3879.707 kb on + strand at 3879.708 kb on - strand at 3879.708 kb on - strand at 3879.712 kb on + strand at 3879.713 kb on - strand at 3879.713 kb on - strand at 3879.725 kb on + strand at 3879.725 kb on + strand at 3879.725 kb on + strand at 3879.726 kb on - strand at 3879.745 kb on + strand at 3879.746 kb on - strand at 3879.746 kb on - strand at 3879.746 kb on - strand at 3879.746 kb on - strand at 3879.762 kb on + strand at 3879.763 kb on - strand at 3879.768 kb on + strand at 3879.768 kb on + strand at 3879.769 kb on - strand at 3879.778 kb on + strand at 3879.783 kb on + strand at 3879.794 kb on + strand at 3879.921 kb on - strand at 3879.927 kb on - strand at 3879.927 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,875,559 + +0.2 3,875,559 + -0.7 3,875,560 - -1.9 3,875,586 + -0.3 3,875,586 + -2.5 3,875,586 + -1.5 3,875,587 - +0.9 3,875,601 + -0.7 3,875,601 + -0.1 3,875,601 + -1.2 3,875,602 - -0.5 3,875,602 - +0.5 3,875,602 - +0.6 3,875,602 - +0.5 3,875,602 - -0.6 3,875,602 - -0.4 3,875,602 - -0.4 3,875,602 - +0.9 3,875,616 + -0.7 3,875,616 + -1.5 3,875,616 + -1.5 3,875,616 + -1.5 3,875,616 + +0.9 3,875,617 - -0.2 3,875,617 - -0.5 3,875,617 - +1.5 3,875,631 + -1.5 3,875,631 + -1.1 3,875,632 - +2.2 3,875,632 - +0.9 3,875,666 + +0.9 3,875,667 - +0.4 3,876,190 + +0.9 3,876,428 + -0.5 3,876,432 + -0.3 3,876,446 - -0.1 3,876,446 - +0.9 3,876,449 - +0.9 3,876,455 + -0.3 3,876,455 + -0.3 3,876,456 - -0.1 3,876,461 + +2.2 3,876,461 + -1.5 3,876,461 + -2.3 3,876,461 + -1.1 3,876,461 + -0.1 3,876,461 + +0.9 3,876,461 + -0.1 3,876,462 - -1.5 3,876,462 - +1.5 3,876,462 - -0.2 3,876,462 - +0.5 3,876,474 + +0.3 3,876,474 + -1.1 3,876,475 - -1.1 3,876,475 - +1.4 3,876,824 + HGI48_RS17315 0.11 +1.2 3,876,875 + HGI48_RS17315 0.13 -1.9 3,876,875 + HGI48_RS17315 0.13 -0.7 3,876,875 + HGI48_RS17315 0.13 +0.9 3,876,979 + HGI48_RS17315 0.18 +1.4 3,876,985 + HGI48_RS17315 0.18 +0.6 3,876,985 + HGI48_RS17315 0.18 +0.3 3,876,985 + HGI48_RS17315 0.18 -1.1 3,876,986 - HGI48_RS17315 0.18 -0.7 3,876,986 - HGI48_RS17315 0.18 -0.3 3,876,988 + HGI48_RS17315 0.18 -1.1 3,876,988 + HGI48_RS17315 0.18 +0.1 3,876,988 + +0.9 3,877,079 + HGI48_RS17315 0.22 +0.6 3,877,079 + HGI48_RS17315 0.22 -0.5 3,877,079 + HGI48_RS17315 0.22 -0.6 3,877,079 + HGI48_RS17315 0.22 +1.1 3,877,079 + HGI48_RS17315 0.22 +0.6 3,877,116 - HGI48_RS17315 0.23 +0.0 3,877,116 - HGI48_RS17315 0.23 +0.4 3,877,140 + HGI48_RS17315 0.24 -1.1 3,877,270 + HGI48_RS17315 0.29 -1.1 3,877,271 - HGI48_RS17315 0.29 -0.3 3,877,271 - +1.9 3,877,271 - HGI48_RS17315 0.29 +0.1 3,877,378 + -0.7 3,877,378 + HGI48_RS17315 0.34 -1.7 3,877,379 - +1.3 3,877,465 + HGI48_RS17315 0.37 +0.3 3,877,652 + HGI48_RS17315 0.45 -0.7 3,877,652 + HGI48_RS17315 0.45 -1.9 3,877,780 + HGI48_RS17315 0.50 -1.7 3,877,780 + HGI48_RS17315 0.50 -1.5 3,877,780 + +1.3 3,877,780 + HGI48_RS17315 0.50 -0.9 3,877,780 + HGI48_RS17315 0.50 -1.7 3,877,780 + HGI48_RS17315 0.50 +2.3 3,877,781 - HGI48_RS17315 0.50 -1.2 3,877,781 - HGI48_RS17315 0.50 +0.3 3,877,781 - HGI48_RS17315 0.50 +1.4 3,877,781 - HGI48_RS17315 0.50 +1.2 3,877,781 - HGI48_RS17315 0.50 -0.4 3,877,781 - HGI48_RS17315 0.50 +0.7 3,877,873 + HGI48_RS17315 0.54 +1.9 3,877,873 + HGI48_RS17315 0.54 +0.4 3,877,873 + HGI48_RS17315 0.54 -1.5 3,877,873 + HGI48_RS17315 0.54 +0.6 3,877,874 - HGI48_RS17315 0.54 -1.7 3,877,874 - HGI48_RS17315 0.54 +1.1 3,877,874 - HGI48_RS17315 0.54 -0.1 3,877,909 + HGI48_RS17315 0.55 +0.1 3,877,909 + HGI48_RS17315 0.55 -1.9 3,877,909 + HGI48_RS17315 0.55 +1.1 3,877,910 - HGI48_RS17315 0.55 -1.9 3,877,910 - -0.1 3,877,916 + HGI48_RS17315 0.55 -1.4 3,877,916 + HGI48_RS17315 0.55 -0.5 3,877,917 - HGI48_RS17315 0.55 -1.9 3,877,965 - HGI48_RS17315 0.57 +0.3 3,877,965 - HGI48_RS17315 0.57 -0.1 3,877,965 - -0.1 3,877,965 - HGI48_RS17315 0.57 -1.1 3,878,050 + HGI48_RS17315 0.61 +0.1 3,878,050 + +2.5 3,878,050 + HGI48_RS17315 0.61 -0.4 3,878,050 + HGI48_RS17315 0.61 +1.5 3,878,050 + HGI48_RS17315 0.61 +2.1 3,878,107 + HGI48_RS17315 0.63 -1.1 3,878,107 + HGI48_RS17315 0.63 +0.6 3,878,107 + HGI48_RS17315 0.63 +0.9 3,878,108 - HGI48_RS17315 0.63 -1.7 3,878,108 - HGI48_RS17315 0.63 +0.0 3,878,110 + HGI48_RS17315 0.63 -0.6 3,878,110 + HGI48_RS17315 0.63 +0.9 3,878,110 + HGI48_RS17315 0.63 -0.1 3,878,110 + +1.3 3,878,110 + HGI48_RS17315 0.63 +2.3 3,878,110 + HGI48_RS17315 0.63 +0.9 3,878,110 + HGI48_RS17315 0.63 -1.5 3,878,111 - HGI48_RS17315 0.63 -1.9 3,878,170 + -0.7 3,878,170 + -0.7 3,878,333 + HGI48_RS17315 0.72 +0.1 3,878,391 + HGI48_RS17315 0.75 -1.9 3,878,391 + HGI48_RS17315 0.75 +0.9 3,878,565 + HGI48_RS17315 0.82 -1.1 3,878,695 + HGI48_RS17315 0.87 +0.5 3,878,696 - HGI48_RS17315 0.87 -2.3 3,878,795 - +1.3 3,878,953 + +0.9 3,878,980 + +2.0 3,879,062 + -1.5 3,879,062 + -1.5 3,879,062 + -1.1 3,879,076 + -1.6 3,879,077 - -2.5 3,879,077 - +1.5 3,879,077 - -0.0 3,879,077 - -0.1 3,879,081 + +0.4 3,879,082 - -0.3 3,879,082 - -1.5 3,879,123 - HGI48_RS17320 0.15 -1.1 3,879,166 + HGI48_RS17320 0.25 -2.1 3,879,193 - HGI48_RS17320 0.31 +0.1 3,879,195 + HGI48_RS17320 0.31 +0.3 3,879,195 + HGI48_RS17320 0.31 -0.0 3,879,196 - HGI48_RS17320 0.32 +0.5 3,879,196 - +0.9 3,879,364 - HGI48_RS17320 0.71 +0.1 3,879,438 + HGI48_RS17320 0.88 -1.5 3,879,438 + HGI48_RS17320 0.88 -2.1 3,879,440 + HGI48_RS17320 0.89 +0.6 3,879,440 + -0.1 3,879,440 + HGI48_RS17320 0.89 -1.3 3,879,441 - HGI48_RS17320 0.89 +1.5 3,879,461 + -0.1 3,879,462 - -2.1 3,879,479 - +3.2 3,879,501 - -0.7 3,879,612 - -1.0 3,879,640 - -3.0 3,879,643 + +0.9 3,879,707 + -0.7 3,879,708 - -0.6 3,879,708 - +2.7 3,879,712 + -0.7 3,879,713 - -0.4 3,879,713 - -2.1 3,879,725 + -1.7 3,879,725 + +1.9 3,879,725 + -1.1 3,879,726 - -0.7 3,879,745 + -0.9 3,879,746 - -0.1 3,879,746 - +3.7 3,879,746 - -0.7 3,879,746 - +0.3 3,879,762 + -1.5 3,879,763 - +1.7 3,879,768 + -0.1 3,879,768 + +0.1 3,879,769 - -1.7 3,879,778 + +0.9 3,879,783 + -0.4 3,879,794 + -0.1 3,879,921 - +0.9 3,879,927 - -2.0 3,879,927 - +0.9
Or see this region's nucleotide sequence