Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS14270 and HGI48_RS14275 are separated by 10 nucleotides HGI48_RS14275 and HGI48_RS14280 are separated by 16 nucleotides HGI48_RS14280 and HGI48_RS14285 overlap by 4 nucleotides
HGI48_RS14270: HGI48_RS14270 - NADH-quinone oxidoreductase subunit J, at 3,204,576 to 3,205,127
_RS14270
HGI48_RS14275: HGI48_RS14275 - NADH-quinone oxidoreductase subunit NuoI, at 3,205,138 to 3,205,680
_RS14275
HGI48_RS14280: HGI48_RS14280 - NADH-quinone oxidoreductase subunit NuoH, at 3,205,697 to 3,206,671
_RS14280
HGI48_RS14285: HGI48_RS14285 - NADH-quinone oxidoreductase subunit NuoG, at 3,206,668 to 3,209,394
_RS14285
Position (kb)
3205
3206
3207 Strain fitness (log2 ratio)
-2
-1
0
1 at 3204.718 kb on + strand, within HGI48_RS14270 at 3204.719 kb on - strand, within HGI48_RS14270 at 3205.042 kb on - strand, within HGI48_RS14270 at 3205.686 kb on - strand at 3205.686 kb on - strand at 3205.947 kb on + strand, within HGI48_RS14280 at 3205.948 kb on - strand, within HGI48_RS14280 at 3206.119 kb on + strand, within HGI48_RS14280 at 3206.120 kb on - strand, within HGI48_RS14280 at 3206.399 kb on - strand, within HGI48_RS14280 at 3206.948 kb on + strand, within HGI48_RS14285 at 3207.269 kb on + strand, within HGI48_RS14285 at 3207.419 kb on + strand, within HGI48_RS14285
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,204,718 + HGI48_RS14270 0.26 -2.2 3,204,719 - HGI48_RS14270 0.26 -2.0 3,205,042 - HGI48_RS14270 0.84 -2.1 3,205,686 - +1.6 3,205,686 - -1.7 3,205,947 + HGI48_RS14280 0.26 +0.6 3,205,948 - HGI48_RS14280 0.26 -0.5 3,206,119 + HGI48_RS14280 0.43 -1.4 3,206,120 - HGI48_RS14280 0.43 +0.4 3,206,399 - HGI48_RS14280 0.72 -1.4 3,206,948 + HGI48_RS14285 0.10 -1.2 3,207,269 + HGI48_RS14285 0.22 +0.2 3,207,419 + HGI48_RS14285 0.28 -0.7
Or see this region's nucleotide sequence