Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS06760 and HGI48_RS06765 are separated by 1 nucleotides HGI48_RS06765 and HGI48_RS06770 overlap by 4 nucleotides HGI48_RS06770 and HGI48_RS06775 are separated by 79 nucleotides HGI48_RS06775 and HGI48_RS06780 overlap by 4 nucleotides
HGI48_RS06760: HGI48_RS06760 - glucose-1-phosphate thymidylyltransferase RfbA, at 1,433,595 to 1,434,464
_RS06760
HGI48_RS06765: HGI48_RS06765 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 1,434,466 to 1,435,002
_RS06765
HGI48_RS06770: HGI48_RS06770 - dTDP-4-dehydrorhamnose reductase, at 1,434,999 to 1,435,856
_RS06770
HGI48_RS06775: HGI48_RS06775 - glycosyltransferase family 2 protein, at 1,435,936 to 1,436,856
_RS06775
HGI48_RS06780: HGI48_RS06780 - oligosaccharide flippase family protein, at 1,436,853 to 1,438,124
_RS06780
Position (kb)
1434
1435
1436 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1434.114 kb on + strand, within HGI48_RS06760 at 1434.114 kb on + strand, within HGI48_RS06760 at 1434.115 kb on - strand, within HGI48_RS06760 at 1434.120 kb on + strand, within HGI48_RS06760 at 1434.120 kb on + strand, within HGI48_RS06760 at 1434.121 kb on - strand, within HGI48_RS06760 at 1434.204 kb on + strand, within HGI48_RS06760 at 1434.204 kb on + strand at 1434.204 kb on + strand, within HGI48_RS06760 at 1434.453 kb on - strand at 1434.465 kb on + strand at 1434.466 kb on - strand at 1434.466 kb on - strand at 1434.499 kb on - strand at 1434.745 kb on + strand, within HGI48_RS06765 at 1434.746 kb on - strand, within HGI48_RS06765 at 1434.764 kb on + strand, within HGI48_RS06765 at 1434.765 kb on - strand, within HGI48_RS06765 at 1434.871 kb on + strand, within HGI48_RS06765 at 1434.871 kb on + strand, within HGI48_RS06765 at 1434.872 kb on - strand, within HGI48_RS06765 at 1434.874 kb on + strand, within HGI48_RS06765 at 1434.874 kb on + strand, within HGI48_RS06765 at 1434.902 kb on + strand, within HGI48_RS06765 at 1434.903 kb on - strand, within HGI48_RS06765 at 1435.033 kb on + strand at 1435.130 kb on + strand, within HGI48_RS06770 at 1435.130 kb on + strand, within HGI48_RS06770 at 1435.283 kb on + strand at 1435.302 kb on + strand, within HGI48_RS06770 at 1435.302 kb on + strand, within HGI48_RS06770 at 1435.302 kb on + strand, within HGI48_RS06770 at 1435.302 kb on + strand, within HGI48_RS06770 at 1435.303 kb on - strand, within HGI48_RS06770 at 1435.303 kb on - strand, within HGI48_RS06770 at 1435.303 kb on - strand, within HGI48_RS06770 at 1435.303 kb on - strand, within HGI48_RS06770 at 1435.303 kb on - strand, within HGI48_RS06770 at 1435.548 kb on + strand, within HGI48_RS06770 at 1435.548 kb on + strand, within HGI48_RS06770 at 1435.548 kb on + strand, within HGI48_RS06770 at 1435.548 kb on + strand, within HGI48_RS06770 at 1435.548 kb on + strand, within HGI48_RS06770 at 1435.549 kb on - strand, within HGI48_RS06770 at 1435.549 kb on - strand, within HGI48_RS06770 at 1435.549 kb on - strand, within HGI48_RS06770 at 1435.611 kb on + strand at 1435.661 kb on + strand, within HGI48_RS06770 at 1435.786 kb on + strand at 1435.836 kb on - strand at 1435.873 kb on + strand at 1435.892 kb on + strand at 1435.893 kb on - strand at 1435.918 kb on + strand at 1435.918 kb on + strand at 1435.919 kb on - strand at 1435.976 kb on - strand at 1435.990 kb on + strand at 1435.991 kb on - strand at 1436.200 kb on + strand, within HGI48_RS06775 at 1436.362 kb on + strand at 1436.449 kb on - strand, within HGI48_RS06775 at 1436.549 kb on - strand at 1436.549 kb on - strand, within HGI48_RS06775 at 1436.852 kb on + strand at 1436.852 kb on + strand at 1436.853 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 1,434,114 + HGI48_RS06760 0.60 -1.7 1,434,114 + HGI48_RS06760 0.60 -1.1 1,434,115 - HGI48_RS06760 0.60 -1.7 1,434,120 + HGI48_RS06760 0.60 -0.2 1,434,120 + HGI48_RS06760 0.60 -0.1 1,434,121 - HGI48_RS06760 0.60 -1.7 1,434,204 + HGI48_RS06760 0.70 +0.5 1,434,204 + -0.4 1,434,204 + HGI48_RS06760 0.70 -0.3 1,434,453 - -0.1 1,434,465 + +0.1 1,434,466 - -0.1 1,434,466 - -1.7 1,434,499 - +0.9 1,434,745 + HGI48_RS06765 0.52 -1.9 1,434,746 - HGI48_RS06765 0.52 +0.2 1,434,764 + HGI48_RS06765 0.55 +1.0 1,434,765 - HGI48_RS06765 0.56 -0.9 1,434,871 + HGI48_RS06765 0.75 +0.4 1,434,871 + HGI48_RS06765 0.75 +0.3 1,434,872 - HGI48_RS06765 0.76 +0.1 1,434,874 + HGI48_RS06765 0.76 +0.2 1,434,874 + HGI48_RS06765 0.76 +0.6 1,434,902 + HGI48_RS06765 0.81 +0.8 1,434,903 - HGI48_RS06765 0.81 +0.9 1,435,033 + +0.2 1,435,130 + HGI48_RS06770 0.15 -2.3 1,435,130 + HGI48_RS06770 0.15 +0.2 1,435,283 + -0.9 1,435,302 + HGI48_RS06770 0.35 -1.9 1,435,302 + HGI48_RS06770 0.35 +0.5 1,435,302 + HGI48_RS06770 0.35 -0.0 1,435,302 + HGI48_RS06770 0.35 +2.3 1,435,303 - HGI48_RS06770 0.35 -0.3 1,435,303 - HGI48_RS06770 0.35 +1.5 1,435,303 - HGI48_RS06770 0.35 -2.7 1,435,303 - HGI48_RS06770 0.35 +1.5 1,435,303 - HGI48_RS06770 0.35 -1.7 1,435,548 + HGI48_RS06770 0.64 -1.7 1,435,548 + HGI48_RS06770 0.64 -0.4 1,435,548 + HGI48_RS06770 0.64 +0.3 1,435,548 + HGI48_RS06770 0.64 +0.3 1,435,548 + HGI48_RS06770 0.64 +0.6 1,435,549 - HGI48_RS06770 0.64 -1.4 1,435,549 - HGI48_RS06770 0.64 +0.2 1,435,549 - HGI48_RS06770 0.64 -0.0 1,435,611 + -0.4 1,435,661 + HGI48_RS06770 0.77 -1.1 1,435,786 + +0.2 1,435,836 - +1.6 1,435,873 + -0.2 1,435,892 + -2.2 1,435,893 - -0.3 1,435,918 + +0.2 1,435,918 + +0.6 1,435,919 - -1.7 1,435,976 - +0.3 1,435,990 + -1.4 1,435,991 - -1.9 1,436,200 + HGI48_RS06775 0.29 -0.1 1,436,362 + -0.9 1,436,449 - HGI48_RS06775 0.56 -2.9 1,436,549 - +0.6 1,436,549 - HGI48_RS06775 0.67 -1.9 1,436,852 + +0.5 1,436,852 + +0.4 1,436,853 - -0.9
Or see this region's nucleotide sequence