Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01145 and HGI48_RS01150 overlap by 1 nucleotides HGI48_RS01150 and HGI48_RS01155 are separated by 1 nucleotides HGI48_RS01155 and HGI48_RS01160 overlap by 4 nucleotides HGI48_RS01160 and HGI48_RS01165 are separated by 1 nucleotides
HGI48_RS01145: HGI48_RS01145 - 2-iminoacetate synthase ThiH, at 258,170 to 259,315
_RS01145
HGI48_RS01150: HGI48_RS01150 - thiazole synthase, at 259,315 to 260,094
_RS01150
HGI48_RS01155: HGI48_RS01155 - sulfur carrier protein ThiS, at 260,096 to 260,296
_RS01155
HGI48_RS01160: HGI48_RS01160 - HesA/MoeB/ThiF family protein, at 260,293 to 261,045
_RS01160
HGI48_RS01165: HGI48_RS01165 - thiamine phosphate synthase, at 261,047 to 261,682
_RS01165
Position (kb) 260
261 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 259.174 kb on + strand at 259.174 kb on + strand, within HGI48_RS01145 at 259.174 kb on + strand, within HGI48_RS01145 at 259.203 kb on - strand at 259.214 kb on + strand at 259.214 kb on + strand at 259.215 kb on - strand at 259.215 kb on - strand at 259.215 kb on - strand at 259.215 kb on - strand at 259.784 kb on + strand at 259.784 kb on + strand, within HGI48_RS01150 at 259.784 kb on + strand, within HGI48_RS01150 at 259.784 kb on + strand, within HGI48_RS01150 at 259.784 kb on + strand, within HGI48_RS01150 at 259.784 kb on + strand, within HGI48_RS01150 at 259.784 kb on + strand at 259.784 kb on + strand, within HGI48_RS01150 at 259.784 kb on + strand, within HGI48_RS01150 at 259.785 kb on - strand, within HGI48_RS01150 at 259.785 kb on - strand, within HGI48_RS01150 at 259.785 kb on - strand, within HGI48_RS01150 at 259.785 kb on - strand, within HGI48_RS01150 at 259.785 kb on - strand, within HGI48_RS01150 at 259.785 kb on - strand, within HGI48_RS01150 at 259.785 kb on - strand, within HGI48_RS01150 at 260.067 kb on + strand at 260.067 kb on + strand at 260.338 kb on + strand at 260.338 kb on + strand at 260.476 kb on + strand, within HGI48_RS01160 at 260.535 kb on - strand, within HGI48_RS01160 at 260.549 kb on + strand, within HGI48_RS01160 at 260.563 kb on + strand, within HGI48_RS01160 at 260.854 kb on + strand, within HGI48_RS01160 at 260.854 kb on + strand at 260.854 kb on + strand at 260.906 kb on + strand, within HGI48_RS01160 at 260.906 kb on + strand, within HGI48_RS01160 at 260.907 kb on - strand, within HGI48_RS01160 at 260.907 kb on - strand, within HGI48_RS01160 at 261.025 kb on - strand at 261.025 kb on - strand at 261.025 kb on - strand at 261.048 kb on + strand at 261.078 kb on - strand at 261.078 kb on - strand at 261.295 kb on + strand, within HGI48_RS01165 at 261.295 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 259,174 + +1.0 259,174 + HGI48_RS01145 0.88 +0.3 259,174 + HGI48_RS01145 0.88 -0.6 259,203 - -0.6 259,214 + -0.2 259,214 + -1.0 259,215 - -1.2 259,215 - +0.2 259,215 - -0.7 259,215 - +1.4 259,784 + +3.2 259,784 + HGI48_RS01150 0.60 +0.7 259,784 + HGI48_RS01150 0.60 -1.8 259,784 + HGI48_RS01150 0.60 +0.6 259,784 + HGI48_RS01150 0.60 -0.8 259,784 + HGI48_RS01150 0.60 -0.6 259,784 + +2.0 259,784 + HGI48_RS01150 0.60 +0.3 259,784 + HGI48_RS01150 0.60 -1.0 259,785 - HGI48_RS01150 0.60 -2.4 259,785 - HGI48_RS01150 0.60 -2.2 259,785 - HGI48_RS01150 0.60 -0.8 259,785 - HGI48_RS01150 0.60 -2.1 259,785 - HGI48_RS01150 0.60 -1.0 259,785 - HGI48_RS01150 0.60 -1.6 259,785 - HGI48_RS01150 0.60 +1.5 260,067 + +2.9 260,067 + -0.4 260,338 + -2.0 260,338 + -0.9 260,476 + HGI48_RS01160 0.24 -0.6 260,535 - HGI48_RS01160 0.32 +1.2 260,549 + HGI48_RS01160 0.34 +0.6 260,563 + HGI48_RS01160 0.36 -0.6 260,854 + HGI48_RS01160 0.75 -1.3 260,854 + +3.0 260,854 + -0.6 260,906 + HGI48_RS01160 0.81 +0.5 260,906 + HGI48_RS01160 0.81 +0.0 260,907 - HGI48_RS01160 0.82 -1.0 260,907 - HGI48_RS01160 0.82 -0.2 261,025 - +0.3 261,025 - -2.0 261,025 - -0.3 261,048 + -2.1 261,078 - +1.2 261,078 - -0.9 261,295 + HGI48_RS01165 0.39 +0.0 261,295 + +1.0
Or see this region's nucleotide sequence