Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS00990 and HGI48_RS00995 are separated by 18 nucleotides HGI48_RS00995 and HGI48_RS01000 are separated by 373 nucleotides HGI48_RS01000 and HGI48_RS01005 are separated by 87 nucleotides HGI48_RS01005 and HGI48_RS01010 are separated by 194 nucleotides
HGI48_RS00990: HGI48_RS00990 - Trk system potassium transporter TrkH, at 224,451 to 225,902
_RS00990
HGI48_RS00995: HGI48_RS00995 - menaquinone-dependent protoporphyrinogen IX dehydrogenase, at 225,921 to 226,460
_RS00995
HGI48_RS01000: HGI48_RS01000 - 16S ribosomal RNA, at 226,834 to 228,378
_RS01000
HGI48_RS01005: HGI48_RS01005 - tRNA-Glu, at 228,466 to 228,541
_RS01005
HGI48_RS01010: HGI48_RS01010 - 23S ribosomal RNA, at 228,736 to 231,661
_RS01010
Position (kb) 226
227
228
229 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 225.875 kb on - strand at 225.875 kb on - strand at 225.879 kb on - strand at 225.879 kb on - strand at 225.900 kb on + strand at 225.920 kb on + strand at 225.920 kb on + strand at 226.458 kb on + strand at 226.458 kb on + strand at 226.459 kb on - strand at 226.549 kb on - strand at 226.645 kb on + strand at 227.624 kb on + strand, within HGI48_RS01000
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 225,875 - -0.6 225,875 - -1.7 225,879 - -1.7 225,879 - -1.0 225,900 + +0.0 225,920 + +0.6 225,920 + -0.3 226,458 + -2.1 226,458 + -1.9 226,459 - -2.7 226,549 - +0.3 226,645 + +3.6 227,624 + HGI48_RS01000 0.51 -0.6
Or see this region's nucleotide sequence