Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS00785 and HGI48_RS00790 are separated by 99 nucleotides HGI48_RS00790 and HGI48_RS00795 overlap by 1 nucleotides HGI48_RS00795 and HGI48_RS00800 are separated by 133 nucleotides
HGI48_RS00785: HGI48_RS00785 - rhodanese-like domain-containing protein, at 179,966 to 180,397
_RS00785
HGI48_RS00790: HGI48_RS00790 - protein-export chaperone SecB, at 180,497 to 180,967
_RS00790
HGI48_RS00795: HGI48_RS00795 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 180,967 to 181,986
_RS00795
HGI48_RS00800: HGI48_RS00800 - serine O-acetyltransferase, at 182,120 to 182,941
_RS00800
Position (kb) 180
181
182 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 179.977 kb on + strand at 179.977 kb on + strand at 179.977 kb on + strand at 180.050 kb on - strand, within HGI48_RS00785 at 180.112 kb on + strand, within HGI48_RS00785 at 180.115 kb on + strand, within HGI48_RS00785 at 180.172 kb on + strand, within HGI48_RS00785 at 180.392 kb on - strand at 180.395 kb on + strand at 180.396 kb on - strand at 180.432 kb on + strand at 180.490 kb on - strand at 180.546 kb on - strand, within HGI48_RS00790 at 180.556 kb on + strand, within HGI48_RS00790 at 180.914 kb on + strand, within HGI48_RS00790 at 180.914 kb on + strand, within HGI48_RS00790 at 181.150 kb on - strand, within HGI48_RS00795 at 181.356 kb on + strand, within HGI48_RS00795 at 181.909 kb on + strand at 181.910 kb on - strand at 181.995 kb on + strand at 181.996 kb on - strand at 181.996 kb on - strand at 181.998 kb on + strand at 181.998 kb on + strand at 181.999 kb on - strand at 182.033 kb on + strand at 182.033 kb on + strand at 182.034 kb on - strand at 182.034 kb on - strand at 182.034 kb on - strand at 182.034 kb on - strand at 182.034 kb on - strand at 182.034 kb on - strand at 182.056 kb on + strand at 182.258 kb on + strand, within HGI48_RS00800 at 182.259 kb on - strand at 182.259 kb on - strand at 182.272 kb on + strand, within HGI48_RS00800 at 182.412 kb on - strand, within HGI48_RS00800 at 182.752 kb on + strand, within HGI48_RS00800 at 182.753 kb on - strand, within HGI48_RS00800 at 182.760 kb on + strand at 182.761 kb on - strand, within HGI48_RS00800 at 182.761 kb on - strand, within HGI48_RS00800 at 182.894 kb on + strand at 182.942 kb on + strand at 182.943 kb on - strand at 182.943 kb on - strand at 182.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 179,977 + -0.8 179,977 + -0.3 179,977 + -0.9 180,050 - HGI48_RS00785 0.19 -0.5 180,112 + HGI48_RS00785 0.34 -0.5 180,115 + HGI48_RS00785 0.34 +0.0 180,172 + HGI48_RS00785 0.48 +1.8 180,392 - -3.6 180,395 + +1.5 180,396 - -0.9 180,432 + -0.9 180,490 - -0.9 180,546 - HGI48_RS00790 0.10 -2.1 180,556 + HGI48_RS00790 0.13 -1.3 180,914 + HGI48_RS00790 0.89 +0.2 180,914 + HGI48_RS00790 0.89 -0.9 181,150 - HGI48_RS00795 0.18 -0.3 181,356 + HGI48_RS00795 0.38 -1.3 181,909 + -1.0 181,910 - +0.1 181,995 + -0.8 181,996 - +0.9 181,996 - +0.5 181,998 + -2.6 181,998 + -0.9 181,999 - -1.7 182,033 + -1.3 182,033 + +0.5 182,034 - -0.6 182,034 - -0.2 182,034 - +1.4 182,034 - +0.1 182,034 - -1.5 182,034 - -0.9 182,056 + +1.7 182,258 + HGI48_RS00800 0.17 -2.6 182,259 - +0.1 182,259 - +1.1 182,272 + HGI48_RS00800 0.18 -1.3 182,412 - HGI48_RS00800 0.36 -1.9 182,752 + HGI48_RS00800 0.77 -2.3 182,753 - HGI48_RS00800 0.77 +1.1 182,760 + +0.1 182,761 - HGI48_RS00800 0.78 -0.5 182,761 - HGI48_RS00800 0.78 -1.7 182,894 + +1.1 182,942 + +0.2 182,943 - -1.5 182,943 - +1.2 182,943 - -2.1
Or see this region's nucleotide sequence