Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS00150 and HGI48_RS00155 are separated by 1 nucleotides HGI48_RS00155 and HGI48_RS00160 are separated by 29 nucleotides HGI48_RS00160 and HGI48_RS00165 are separated by 323 nucleotides
HGI48_RS00150: HGI48_RS00150 - ABC transporter permease, at 38,474 to 39,349
_RS00150
HGI48_RS00155: HGI48_RS00155 - ABC transporter permease, at 39,351 to 40,292
_RS00155
HGI48_RS00160: HGI48_RS00160 - TIGR04028 family ABC transporter substrate-binding protein, at 40,322 to 41,965
_RS00160
HGI48_RS00165: HGI48_RS00165 - valine--pyruvate transaminase, at 42,289 to 43,545
_RS00165
Position (kb)
40
41
42 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 39.349 kb on + strand at 39.349 kb on + strand at 39.349 kb on + strand at 39.350 kb on - strand at 39.462 kb on + strand, within HGI48_RS00155 at 39.462 kb on + strand, within HGI48_RS00155 at 39.463 kb on - strand, within HGI48_RS00155 at 39.463 kb on - strand, within HGI48_RS00155 at 40.001 kb on + strand, within HGI48_RS00155 at 40.118 kb on - strand, within HGI48_RS00155 at 40.153 kb on - strand, within HGI48_RS00155 at 40.209 kb on + strand at 40.229 kb on + strand at 40.229 kb on + strand at 40.229 kb on + strand at 40.230 kb on - strand at 40.279 kb on + strand at 40.279 kb on + strand at 40.280 kb on - strand at 40.280 kb on - strand at 40.280 kb on - strand at 40.280 kb on - strand at 40.323 kb on + strand at 40.344 kb on + strand at 40.345 kb on - strand at 40.437 kb on + strand at 40.437 kb on + strand at 40.437 kb on + strand at 40.437 kb on + strand at 40.438 kb on - strand at 40.438 kb on - strand at 40.438 kb on - strand at 40.438 kb on - strand at 40.455 kb on + strand at 40.455 kb on + strand at 40.455 kb on + strand at 40.456 kb on - strand at 40.609 kb on - strand, within HGI48_RS00160 at 40.611 kb on + strand, within HGI48_RS00160 at 40.611 kb on + strand, within HGI48_RS00160 at 40.612 kb on - strand, within HGI48_RS00160 at 40.625 kb on + strand, within HGI48_RS00160 at 40.625 kb on + strand, within HGI48_RS00160 at 40.626 kb on - strand, within HGI48_RS00160 at 40.977 kb on + strand, within HGI48_RS00160 at 40.989 kb on + strand, within HGI48_RS00160 at 40.989 kb on + strand, within HGI48_RS00160 at 41.118 kb on + strand, within HGI48_RS00160 at 41.119 kb on - strand, within HGI48_RS00160 at 41.157 kb on + strand, within HGI48_RS00160 at 41.157 kb on + strand, within HGI48_RS00160 at 41.157 kb on + strand, within HGI48_RS00160 at 41.238 kb on + strand, within HGI48_RS00160 at 41.238 kb on + strand, within HGI48_RS00160 at 41.238 kb on + strand, within HGI48_RS00160 at 41.239 kb on - strand, within HGI48_RS00160 at 41.239 kb on - strand at 41.239 kb on - strand, within HGI48_RS00160 at 41.239 kb on - strand, within HGI48_RS00160 at 41.239 kb on - strand, within HGI48_RS00160 at 41.239 kb on - strand, within HGI48_RS00160 at 41.239 kb on - strand, within HGI48_RS00160 at 41.239 kb on - strand at 41.239 kb on - strand, within HGI48_RS00160 at 41.298 kb on + strand, within HGI48_RS00160 at 41.487 kb on + strand at 41.487 kb on + strand, within HGI48_RS00160 at 41.488 kb on - strand, within HGI48_RS00160 at 41.529 kb on + strand, within HGI48_RS00160 at 41.529 kb on + strand, within HGI48_RS00160 at 41.529 kb on + strand, within HGI48_RS00160 at 41.529 kb on + strand, within HGI48_RS00160 at 41.529 kb on + strand, within HGI48_RS00160 at 41.530 kb on - strand, within HGI48_RS00160 at 41.530 kb on - strand, within HGI48_RS00160 at 41.530 kb on - strand, within HGI48_RS00160 at 41.530 kb on - strand, within HGI48_RS00160 at 41.667 kb on + strand, within HGI48_RS00160 at 41.668 kb on - strand, within HGI48_RS00160 at 41.668 kb on - strand at 41.742 kb on + strand, within HGI48_RS00160 at 41.742 kb on + strand, within HGI48_RS00160 at 41.742 kb on + strand, within HGI48_RS00160 at 41.743 kb on - strand, within HGI48_RS00160 at 41.758 kb on + strand at 41.806 kb on + strand at 41.806 kb on + strand at 41.806 kb on + strand at 41.807 kb on - strand at 41.808 kb on + strand at 41.808 kb on + strand at 41.809 kb on - strand at 41.809 kb on - strand at 41.809 kb on - strand at 41.809 kb on - strand at 41.838 kb on + strand at 41.839 kb on - strand at 41.839 kb on - strand at 41.900 kb on + strand at 41.921 kb on + strand at 41.922 kb on - strand at 41.979 kb on + strand at 42.058 kb on - strand at 42.058 kb on - strand at 42.058 kb on - strand at 42.082 kb on + strand at 42.082 kb on + strand at 42.082 kb on + strand at 42.083 kb on - strand at 42.083 kb on - strand at 42.099 kb on - strand at 42.112 kb on + strand at 42.112 kb on + strand at 42.112 kb on + strand at 42.112 kb on + strand at 42.112 kb on + strand at 42.112 kb on + strand at 42.113 kb on - strand at 42.113 kb on - strand at 42.113 kb on - strand at 42.116 kb on + strand at 42.116 kb on + strand at 42.116 kb on + strand at 42.116 kb on + strand at 42.117 kb on - strand at 42.117 kb on - strand at 42.182 kb on - strand at 42.182 kb on - strand at 42.190 kb on - strand at 42.190 kb on - strand at 42.190 kb on - strand at 42.193 kb on + strand at 42.193 kb on + strand at 42.194 kb on - strand at 42.194 kb on - strand at 42.194 kb on - strand at 42.250 kb on + strand at 42.374 kb on + strand at 42.374 kb on + strand at 42.374 kb on + strand at 42.374 kb on + strand at 42.374 kb on + strand at 42.374 kb on + strand at 42.374 kb on + strand at 42.374 kb on + strand at 42.374 kb on + strand at 42.374 kb on + strand at 42.374 kb on + strand at 42.374 kb on + strand at 42.374 kb on + strand at 42.375 kb on - strand at 42.375 kb on - strand at 42.375 kb on - strand at 42.375 kb on - strand at 42.375 kb on - strand at 42.375 kb on - strand at 42.375 kb on - strand at 42.375 kb on - strand at 42.434 kb on + strand at 42.434 kb on + strand, within HGI48_RS00165 at 42.434 kb on + strand at 42.435 kb on - strand, within HGI48_RS00165 at 42.435 kb on - strand, within HGI48_RS00165 at 42.435 kb on - strand, within HGI48_RS00165 at 42.479 kb on + strand, within HGI48_RS00165 at 42.576 kb on - strand, within HGI48_RS00165 at 42.618 kb on - strand, within HGI48_RS00165 at 42.861 kb on + strand at 42.862 kb on - strand, within HGI48_RS00165 at 42.884 kb on + strand, within HGI48_RS00165
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 39,349 + +0.2 39,349 + -1.8 39,349 + +1.1 39,350 - -1.3 39,462 + HGI48_RS00155 0.12 -0.7 39,462 + HGI48_RS00155 0.12 +1.1 39,463 - HGI48_RS00155 0.12 +1.1 39,463 - HGI48_RS00155 0.12 +1.3 40,001 + HGI48_RS00155 0.69 +0.8 40,118 - HGI48_RS00155 0.81 +0.8 40,153 - HGI48_RS00155 0.85 -3.3 40,209 + -0.3 40,229 + -0.0 40,229 + -2.8 40,229 + +0.7 40,230 - +0.7 40,279 + +0.5 40,279 + +0.1 40,280 - +0.5 40,280 - +0.1 40,280 - +1.1 40,280 - +0.1 40,323 + +1.5 40,344 + +1.1 40,345 - -0.5 40,437 + -1.9 40,437 + -0.9 40,437 + -0.3 40,437 + -1.8 40,438 - -2.1 40,438 - +0.1 40,438 - +0.5 40,438 - -0.5 40,455 + -1.8 40,455 + -0.5 40,455 + -0.4 40,456 - +0.9 40,609 - HGI48_RS00160 0.17 -0.0 40,611 + HGI48_RS00160 0.18 +1.9 40,611 + HGI48_RS00160 0.18 -0.4 40,612 - HGI48_RS00160 0.18 -0.9 40,625 + HGI48_RS00160 0.18 -0.5 40,625 + HGI48_RS00160 0.18 -1.3 40,626 - HGI48_RS00160 0.18 -0.8 40,977 + HGI48_RS00160 0.40 -1.3 40,989 + HGI48_RS00160 0.41 -1.3 40,989 + HGI48_RS00160 0.41 -0.2 41,118 + HGI48_RS00160 0.48 -0.3 41,119 - HGI48_RS00160 0.48 +0.0 41,157 + HGI48_RS00160 0.51 -0.4 41,157 + HGI48_RS00160 0.51 -2.3 41,157 + HGI48_RS00160 0.51 +0.1 41,238 + HGI48_RS00160 0.56 +2.5 41,238 + HGI48_RS00160 0.56 -1.8 41,238 + HGI48_RS00160 0.56 -0.1 41,239 - HGI48_RS00160 0.56 +0.3 41,239 - +0.5 41,239 - HGI48_RS00160 0.56 -0.3 41,239 - HGI48_RS00160 0.56 -1.4 41,239 - HGI48_RS00160 0.56 +0.7 41,239 - HGI48_RS00160 0.56 -1.9 41,239 - HGI48_RS00160 0.56 +0.2 41,239 - +1.5 41,239 - HGI48_RS00160 0.56 -1.3 41,298 + HGI48_RS00160 0.59 +0.0 41,487 + -0.5 41,487 + HGI48_RS00160 0.71 -1.5 41,488 - HGI48_RS00160 0.71 -1.9 41,529 + HGI48_RS00160 0.73 -1.3 41,529 + HGI48_RS00160 0.73 -0.2 41,529 + HGI48_RS00160 0.73 +0.1 41,529 + HGI48_RS00160 0.73 -0.8 41,529 + HGI48_RS00160 0.73 +0.3 41,530 - HGI48_RS00160 0.73 +0.7 41,530 - HGI48_RS00160 0.73 +0.2 41,530 - HGI48_RS00160 0.73 +0.4 41,530 - HGI48_RS00160 0.73 -0.2 41,667 + HGI48_RS00160 0.82 -0.9 41,668 - HGI48_RS00160 0.82 -1.8 41,668 - -0.5 41,742 + HGI48_RS00160 0.86 -0.3 41,742 + HGI48_RS00160 0.86 +1.3 41,742 + HGI48_RS00160 0.86 +0.1 41,743 - HGI48_RS00160 0.86 -0.3 41,758 + -0.5 41,806 + +0.5 41,806 + -1.2 41,806 + -0.2 41,807 - +0.1 41,808 + +0.1 41,808 + +0.9 41,809 - -0.2 41,809 - +0.6 41,809 - +0.6 41,809 - +0.1 41,838 + +2.1 41,839 - +0.5 41,839 - +0.2 41,900 + -0.3 41,921 + +0.2 41,922 - +0.1 41,979 + +0.1 42,058 - +0.5 42,058 - +0.9 42,058 - +0.3 42,082 + +1.1 42,082 + -0.4 42,082 + -0.9 42,083 - +0.3 42,083 - -0.4 42,099 - -0.9 42,112 + +1.1 42,112 + +2.1 42,112 + +1.5 42,112 + -1.8 42,112 + -2.5 42,112 + -1.5 42,113 - -1.5 42,113 - -1.1 42,113 - -0.9 42,116 + +0.1 42,116 + +0.5 42,116 + +0.5 42,116 + +1.5 42,117 - +0.9 42,117 - -2.4 42,182 - +2.6 42,182 - -2.3 42,190 - +0.8 42,190 - -0.4 42,190 - -1.5 42,193 + -1.6 42,193 + -0.8 42,194 - +0.1 42,194 - -0.4 42,194 - +0.4 42,250 + +2.8 42,374 + +0.1 42,374 + +1.1 42,374 + -0.1 42,374 + +1.9 42,374 + +0.5 42,374 + -1.2 42,374 + +1.6 42,374 + -1.5 42,374 + +0.3 42,374 + +0.1 42,374 + -0.9 42,374 + +1.1 42,374 + -3.1 42,375 - -1.8 42,375 - +0.1 42,375 - -0.0 42,375 - -0.6 42,375 - -0.5 42,375 - +0.5 42,375 - +1.1 42,375 - +0.1 42,434 + +2.6 42,434 + HGI48_RS00165 0.12 -0.7 42,434 + +0.5 42,435 - HGI48_RS00165 0.12 -0.5 42,435 - HGI48_RS00165 0.12 +0.8 42,435 - HGI48_RS00165 0.12 -0.1 42,479 + HGI48_RS00165 0.15 -1.8 42,576 - HGI48_RS00165 0.23 -1.3 42,618 - HGI48_RS00165 0.26 -1.8 42,861 + +0.1 42,862 - HGI48_RS00165 0.46 +0.5 42,884 + HGI48_RS00165 0.47 -2.4
Or see this region's nucleotide sequence