Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS18335 and HGI48_RS18340 are separated by 3 nucleotides HGI48_RS18340 and HGI48_RS18345 are separated by 2 nucleotides
HGI48_RS18335: HGI48_RS18335 - threonine synthase, at 4,114,958 to 4,116,247
_RS18335
HGI48_RS18340: HGI48_RS18340 - homoserine kinase, at 4,116,251 to 4,117,180
_RS18340
HGI48_RS18345: HGI48_RS18345 - bifunctional aspartate kinase/homoserine dehydrogenase I, at 4,117,183 to 4,119,639
_RS18345
Position (kb)
4116
4117
4118 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4115.533 kb on + strand, within HGI48_RS18335 at 4115.533 kb on + strand, within HGI48_RS18335 at 4115.534 kb on - strand, within HGI48_RS18335 at 4115.572 kb on - strand, within HGI48_RS18335 at 4115.572 kb on - strand, within HGI48_RS18335 at 4115.574 kb on + strand, within HGI48_RS18335 at 4115.574 kb on + strand, within HGI48_RS18335 at 4115.734 kb on + strand, within HGI48_RS18335 at 4115.778 kb on + strand, within HGI48_RS18335 at 4115.811 kb on + strand, within HGI48_RS18335 at 4115.868 kb on - strand, within HGI48_RS18335 at 4116.237 kb on + strand at 4116.238 kb on - strand at 4116.238 kb on - strand at 4116.238 kb on - strand at 4116.292 kb on + strand at 4116.292 kb on + strand at 4116.292 kb on + strand at 4116.292 kb on + strand at 4116.292 kb on + strand at 4116.292 kb on + strand at 4116.293 kb on - strand at 4116.293 kb on - strand at 4116.293 kb on - strand at 4116.293 kb on - strand at 4116.293 kb on - strand at 4116.321 kb on + strand at 4116.322 kb on - strand at 4116.322 kb on - strand at 4116.762 kb on + strand, within HGI48_RS18340 at 4116.762 kb on + strand, within HGI48_RS18340 at 4116.984 kb on + strand, within HGI48_RS18340 at 4117.048 kb on + strand, within HGI48_RS18340 at 4117.049 kb on - strand, within HGI48_RS18340 at 4117.167 kb on + strand at 4117.167 kb on + strand at 4117.209 kb on + strand at 4117.210 kb on - strand at 4117.292 kb on + strand at 4117.295 kb on + strand at 4117.296 kb on - strand at 4117.304 kb on + strand at 4117.409 kb on + strand at 4117.409 kb on + strand at 4117.409 kb on + strand at 4117.409 kb on + strand at 4117.414 kb on + strand at 4117.422 kb on + strand at 4117.422 kb on + strand at 4117.512 kb on + strand, within HGI48_RS18345 at 4117.512 kb on + strand, within HGI48_RS18345 at 4117.512 kb on + strand at 4117.513 kb on - strand, within HGI48_RS18345 at 4117.513 kb on - strand, within HGI48_RS18345 at 4117.566 kb on - strand, within HGI48_RS18345 at 4117.713 kb on + strand, within HGI48_RS18345 at 4117.738 kb on - strand at 4117.826 kb on + strand, within HGI48_RS18345 at 4117.826 kb on + strand at 4117.827 kb on - strand, within HGI48_RS18345 at 4117.827 kb on - strand, within HGI48_RS18345 at 4117.836 kb on + strand, within HGI48_RS18345 at 4117.836 kb on + strand at 4117.874 kb on + strand, within HGI48_RS18345 at 4117.875 kb on - strand, within HGI48_RS18345 at 4117.877 kb on + strand, within HGI48_RS18345 at 4117.878 kb on - strand, within HGI48_RS18345 at 4117.950 kb on - strand, within HGI48_RS18345 at 4117.977 kb on + strand, within HGI48_RS18345 at 4117.978 kb on - strand, within HGI48_RS18345 at 4118.009 kb on + strand, within HGI48_RS18345 at 4118.009 kb on + strand, within HGI48_RS18345 at 4118.010 kb on - strand, within HGI48_RS18345 at 4118.010 kb on - strand, within HGI48_RS18345 at 4118.089 kb on - strand, within HGI48_RS18345 at 4118.089 kb on - strand, within HGI48_RS18345 at 4118.101 kb on + strand, within HGI48_RS18345 at 4118.102 kb on - strand, within HGI48_RS18345 at 4118.102 kb on - strand, within HGI48_RS18345 at 4118.102 kb on - strand, within HGI48_RS18345 at 4118.102 kb on - strand, within HGI48_RS18345
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 4,115,533 + HGI48_RS18335 0.45 -0.6 4,115,533 + HGI48_RS18335 0.45 -1.9 4,115,534 - HGI48_RS18335 0.45 -2.3 4,115,572 - HGI48_RS18335 0.48 -1.5 4,115,572 - HGI48_RS18335 0.48 -2.6 4,115,574 + HGI48_RS18335 0.48 -1.2 4,115,574 + HGI48_RS18335 0.48 -1.3 4,115,734 + HGI48_RS18335 0.60 -1.1 4,115,778 + HGI48_RS18335 0.64 -0.6 4,115,811 + HGI48_RS18335 0.66 -0.7 4,115,868 - HGI48_RS18335 0.71 -0.2 4,116,237 + -2.1 4,116,238 - -0.1 4,116,238 - +0.7 4,116,238 - -1.9 4,116,292 + -0.3 4,116,292 + +0.3 4,116,292 + +0.7 4,116,292 + +0.1 4,116,292 + -1.1 4,116,292 + +2.0 4,116,293 - -1.3 4,116,293 - -0.9 4,116,293 - -1.3 4,116,293 - +1.3 4,116,293 - +1.0 4,116,321 + -1.4 4,116,322 - -1.6 4,116,322 - -1.3 4,116,762 + HGI48_RS18340 0.55 -1.6 4,116,762 + HGI48_RS18340 0.55 -1.1 4,116,984 + HGI48_RS18340 0.79 -1.3 4,117,048 + HGI48_RS18340 0.86 -1.6 4,117,049 - HGI48_RS18340 0.86 -1.9 4,117,167 + -0.3 4,117,167 + -1.3 4,117,209 + +1.7 4,117,210 - -0.3 4,117,292 + -2.1 4,117,295 + +0.6 4,117,296 - -0.3 4,117,304 + +0.7 4,117,409 + -2.9 4,117,409 + -2.6 4,117,409 + +0.4 4,117,409 + -0.5 4,117,414 + +0.4 4,117,422 + -1.6 4,117,422 + +1.1 4,117,512 + HGI48_RS18345 0.13 -0.9 4,117,512 + HGI48_RS18345 0.13 -2.4 4,117,512 + +1.1 4,117,513 - HGI48_RS18345 0.13 -1.9 4,117,513 - HGI48_RS18345 0.13 -1.6 4,117,566 - HGI48_RS18345 0.16 -3.2 4,117,713 + HGI48_RS18345 0.22 -4.2 4,117,738 - +0.7 4,117,826 + HGI48_RS18345 0.26 +1.2 4,117,826 + -0.3 4,117,827 - HGI48_RS18345 0.26 -1.0 4,117,827 - HGI48_RS18345 0.26 +1.3 4,117,836 + HGI48_RS18345 0.27 +0.3 4,117,836 + +2.5 4,117,874 + HGI48_RS18345 0.28 +0.0 4,117,875 - HGI48_RS18345 0.28 -1.9 4,117,877 + HGI48_RS18345 0.28 -0.9 4,117,878 - HGI48_RS18345 0.28 -0.6 4,117,950 - HGI48_RS18345 0.31 +0.9 4,117,977 + HGI48_RS18345 0.32 -0.9 4,117,978 - HGI48_RS18345 0.32 -2.3 4,118,009 + HGI48_RS18345 0.34 +0.7 4,118,009 + HGI48_RS18345 0.34 -1.8 4,118,010 - HGI48_RS18345 0.34 +0.7 4,118,010 - HGI48_RS18345 0.34 +1.1 4,118,089 - HGI48_RS18345 0.37 -1.5 4,118,089 - HGI48_RS18345 0.37 +0.4 4,118,101 + HGI48_RS18345 0.37 -1.3 4,118,102 - HGI48_RS18345 0.37 -1.6 4,118,102 - HGI48_RS18345 0.37 -1.3 4,118,102 - HGI48_RS18345 0.37 +0.1 4,118,102 - HGI48_RS18345 0.37 -1.6
Or see this region's nucleotide sequence