Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS18170 and HGI48_RS18175 are separated by 63 nucleotides HGI48_RS18175 and HGI48_RS18180 are separated by 64 nucleotides
HGI48_RS18170: HGI48_RS18170 - RNA polymerase-associated protein RapA, at 4,075,152 to 4,078,055
_RS18170
HGI48_RS18175: HGI48_RS18175 - bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA, at 4,078,119 to 4,078,772
_RS18175
HGI48_RS18180: HGI48_RS18180 - co-chaperone DjlA, at 4,078,837 to 4,079,859
_RS18180
Position (kb)
4078
4079 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4077.146 kb on + strand, within HGI48_RS18170 at 4077.147 kb on - strand, within HGI48_RS18170 at 4077.147 kb on - strand at 4077.278 kb on + strand, within HGI48_RS18170 at 4077.279 kb on - strand, within HGI48_RS18170 at 4077.383 kb on + strand, within HGI48_RS18170 at 4077.383 kb on + strand, within HGI48_RS18170 at 4077.384 kb on - strand, within HGI48_RS18170 at 4077.384 kb on - strand, within HGI48_RS18170 at 4077.384 kb on - strand, within HGI48_RS18170 at 4077.384 kb on - strand, within HGI48_RS18170 at 4077.604 kb on + strand, within HGI48_RS18170 at 4077.606 kb on + strand, within HGI48_RS18170 at 4077.606 kb on + strand, within HGI48_RS18170 at 4077.606 kb on + strand, within HGI48_RS18170 at 4077.606 kb on + strand, within HGI48_RS18170 at 4077.606 kb on + strand, within HGI48_RS18170 at 4077.606 kb on + strand, within HGI48_RS18170 at 4077.606 kb on + strand, within HGI48_RS18170 at 4077.606 kb on + strand, within HGI48_RS18170 at 4077.607 kb on - strand, within HGI48_RS18170 at 4077.607 kb on - strand, within HGI48_RS18170 at 4077.607 kb on - strand, within HGI48_RS18170 at 4077.607 kb on - strand, within HGI48_RS18170 at 4077.607 kb on - strand, within HGI48_RS18170 at 4077.607 kb on - strand, within HGI48_RS18170 at 4077.607 kb on - strand, within HGI48_RS18170 at 4078.053 kb on + strand at 4078.054 kb on - strand at 4078.054 kb on - strand at 4078.071 kb on + strand at 4078.072 kb on - strand at 4078.075 kb on - strand at 4078.075 kb on - strand at 4078.075 kb on - strand at 4078.128 kb on + strand at 4078.128 kb on + strand at 4078.129 kb on - strand at 4078.129 kb on - strand at 4078.398 kb on + strand, within HGI48_RS18175 at 4078.444 kb on + strand, within HGI48_RS18175 at 4078.529 kb on + strand, within HGI48_RS18175 at 4078.529 kb on + strand, within HGI48_RS18175 at 4078.530 kb on - strand at 4078.530 kb on - strand, within HGI48_RS18175 at 4078.604 kb on - strand, within HGI48_RS18175 at 4078.653 kb on + strand, within HGI48_RS18175 at 4078.896 kb on + strand at 4078.896 kb on + strand at 4078.980 kb on - strand at 4079.057 kb on + strand, within HGI48_RS18180 at 4079.058 kb on - strand, within HGI48_RS18180 at 4079.058 kb on - strand, within HGI48_RS18180 at 4079.195 kb on + strand, within HGI48_RS18180 at 4079.195 kb on + strand, within HGI48_RS18180 at 4079.267 kb on + strand at 4079.267 kb on + strand, within HGI48_RS18180 at 4079.335 kb on - strand, within HGI48_RS18180 at 4079.393 kb on + strand, within HGI48_RS18180 at 4079.393 kb on + strand, within HGI48_RS18180 at 4079.393 kb on + strand, within HGI48_RS18180 at 4079.393 kb on + strand, within HGI48_RS18180 at 4079.394 kb on - strand at 4079.394 kb on - strand, within HGI48_RS18180 at 4079.394 kb on - strand, within HGI48_RS18180 at 4079.447 kb on - strand, within HGI48_RS18180
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 4,077,146 + HGI48_RS18170 0.69 -0.0 4,077,147 - HGI48_RS18170 0.69 -0.2 4,077,147 - +0.1 4,077,278 + HGI48_RS18170 0.73 +0.5 4,077,279 - HGI48_RS18170 0.73 -1.6 4,077,383 + HGI48_RS18170 0.77 -1.3 4,077,383 + HGI48_RS18170 0.77 -1.3 4,077,384 - HGI48_RS18170 0.77 +0.7 4,077,384 - HGI48_RS18170 0.77 +0.4 4,077,384 - HGI48_RS18170 0.77 -0.7 4,077,384 - HGI48_RS18170 0.77 -0.9 4,077,604 + HGI48_RS18170 0.84 -1.9 4,077,606 + HGI48_RS18170 0.85 +0.3 4,077,606 + HGI48_RS18170 0.85 +2.4 4,077,606 + HGI48_RS18170 0.85 -1.3 4,077,606 + HGI48_RS18170 0.85 -1.6 4,077,606 + HGI48_RS18170 0.85 -0.9 4,077,606 + HGI48_RS18170 0.85 -1.3 4,077,606 + HGI48_RS18170 0.85 -0.1 4,077,606 + HGI48_RS18170 0.85 +1.0 4,077,607 - HGI48_RS18170 0.85 +1.0 4,077,607 - HGI48_RS18170 0.85 -0.6 4,077,607 - HGI48_RS18170 0.85 +0.9 4,077,607 - HGI48_RS18170 0.85 -0.2 4,077,607 - HGI48_RS18170 0.85 -3.0 4,077,607 - HGI48_RS18170 0.85 -1.3 4,077,607 - HGI48_RS18170 0.85 +0.3 4,078,053 + +0.2 4,078,054 - -0.6 4,078,054 - -1.6 4,078,071 + -0.3 4,078,072 - -0.9 4,078,075 - -2.0 4,078,075 - -0.8 4,078,075 - -1.6 4,078,128 + +1.3 4,078,128 + +2.3 4,078,129 - -1.6 4,078,129 - +1.1 4,078,398 + HGI48_RS18175 0.43 +0.3 4,078,444 + HGI48_RS18175 0.50 -0.9 4,078,529 + HGI48_RS18175 0.63 -1.6 4,078,529 + HGI48_RS18175 0.63 -1.3 4,078,530 - +0.7 4,078,530 - HGI48_RS18175 0.63 -1.3 4,078,604 - HGI48_RS18175 0.74 -2.1 4,078,653 + HGI48_RS18175 0.82 -1.6 4,078,896 + -1.3 4,078,896 + -0.1 4,078,980 - +3.0 4,079,057 + HGI48_RS18180 0.22 -0.6 4,079,058 - HGI48_RS18180 0.22 +0.5 4,079,058 - HGI48_RS18180 0.22 -0.3 4,079,195 + HGI48_RS18180 0.35 -1.6 4,079,195 + HGI48_RS18180 0.35 -1.3 4,079,267 + +1.7 4,079,267 + HGI48_RS18180 0.42 +1.3 4,079,335 - HGI48_RS18180 0.49 +0.3 4,079,393 + HGI48_RS18180 0.54 -0.6 4,079,393 + HGI48_RS18180 0.54 -0.4 4,079,393 + HGI48_RS18180 0.54 -1.3 4,079,393 + HGI48_RS18180 0.54 +0.8 4,079,394 - -0.3 4,079,394 - HGI48_RS18180 0.54 +0.2 4,079,394 - HGI48_RS18180 0.54 -1.9 4,079,447 - HGI48_RS18180 0.60 -0.2
Or see this region's nucleotide sequence