Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS16310 and HGI48_RS16315 overlap by 4 nucleotides HGI48_RS16315 and HGI48_RS16320 are separated by 177 nucleotides HGI48_RS16320 and HGI48_RS16325 are separated by 57 nucleotides HGI48_RS16325 and HGI48_RS16330 are separated by 76 nucleotides HGI48_RS16330 and HGI48_RS16335 are separated by 7 nucleotides HGI48_RS16335 and HGI48_RS16340 are separated by 305 nucleotides
HGI48_RS16310: HGI48_RS16310 - DedA family protein, at 3,657,624 to 3,658,052
_RS16310
HGI48_RS16315: HGI48_RS16315 - fructose-1-phosphate/6-phosphogluconate phosphatase, at 3,658,049 to 3,658,615
_RS16315
HGI48_RS16320: HGI48_RS16320 - tRNA-Arg, at 3,658,793 to 3,658,869
_RS16320
HGI48_RS16325: HGI48_RS16325 - tRNA-Arg, at 3,658,927 to 3,659,003
_RS16325
HGI48_RS16330: HGI48_RS16330 - tRNA-Arg, at 3,659,080 to 3,659,156
_RS16330
HGI48_RS16335: HGI48_RS16335 - tRNA-Ser, at 3,659,164 to 3,659,256
_RS16335
HGI48_RS16340: HGI48_RS16340 - carbon storage regulator CsrA, at 3,659,562 to 3,659,747
_RS16340
Position (kb)
3658
3659 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3657.849 kb on - strand, within HGI48_RS16310 at 3657.875 kb on + strand, within HGI48_RS16310 at 3657.876 kb on - strand at 3657.899 kb on + strand at 3657.900 kb on - strand, within HGI48_RS16310 at 3657.900 kb on - strand, within HGI48_RS16310 at 3657.900 kb on - strand, within HGI48_RS16310 at 3657.902 kb on + strand, within HGI48_RS16310 at 3657.903 kb on - strand, within HGI48_RS16310 at 3657.903 kb on - strand, within HGI48_RS16310 at 3657.918 kb on - strand, within HGI48_RS16310 at 3657.918 kb on - strand, within HGI48_RS16310 at 3657.951 kb on + strand, within HGI48_RS16310 at 3658.061 kb on + strand at 3658.061 kb on + strand at 3658.062 kb on - strand at 3658.062 kb on - strand at 3658.072 kb on + strand at 3658.078 kb on + strand at 3658.078 kb on + strand at 3658.154 kb on + strand, within HGI48_RS16315 at 3658.154 kb on + strand, within HGI48_RS16315 at 3658.155 kb on - strand, within HGI48_RS16315 at 3658.192 kb on + strand, within HGI48_RS16315 at 3658.193 kb on - strand, within HGI48_RS16315 at 3658.320 kb on + strand, within HGI48_RS16315 at 3658.320 kb on + strand, within HGI48_RS16315 at 3658.320 kb on + strand, within HGI48_RS16315 at 3658.321 kb on - strand, within HGI48_RS16315 at 3658.321 kb on - strand at 3658.356 kb on - strand at 3658.356 kb on - strand, within HGI48_RS16315 at 3658.356 kb on - strand, within HGI48_RS16315 at 3658.409 kb on - strand, within HGI48_RS16315 at 3658.441 kb on + strand, within HGI48_RS16315 at 3658.442 kb on - strand, within HGI48_RS16315 at 3658.503 kb on + strand, within HGI48_RS16315 at 3658.504 kb on - strand, within HGI48_RS16315 at 3658.510 kb on + strand, within HGI48_RS16315 at 3658.510 kb on + strand, within HGI48_RS16315 at 3658.511 kb on - strand, within HGI48_RS16315 at 3658.511 kb on - strand at 3658.515 kb on + strand, within HGI48_RS16315 at 3658.515 kb on + strand, within HGI48_RS16315 at 3658.515 kb on + strand, within HGI48_RS16315 at 3658.516 kb on - strand, within HGI48_RS16315 at 3658.516 kb on - strand, within HGI48_RS16315 at 3658.611 kb on + strand at 3658.611 kb on + strand at 3658.612 kb on - strand at 3658.612 kb on - strand at 3658.648 kb on - strand at 3658.712 kb on + strand at 3658.781 kb on + strand at 3658.843 kb on + strand, within HGI48_RS16320 at 3658.843 kb on + strand, within HGI48_RS16320 at 3658.843 kb on + strand, within HGI48_RS16320 at 3658.843 kb on + strand, within HGI48_RS16320 at 3658.848 kb on + strand, within HGI48_RS16320 at 3658.874 kb on + strand at 3658.886 kb on - strand at 3658.893 kb on + strand at 3658.898 kb on + strand at 3658.899 kb on - strand at 3658.918 kb on + strand at 3659.031 kb on + strand at 3659.038 kb on + strand at 3659.039 kb on - strand at 3659.077 kb on - strand at 3659.478 kb on + strand at 3659.502 kb on - strand at 3659.556 kb on - strand at 3659.558 kb on + strand at 3659.559 kb on - strand at 3659.559 kb on - strand at 3659.566 kb on + strand at 3659.566 kb on + strand at 3659.567 kb on - strand at 3659.574 kb on - strand at 3659.770 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,657,849 - HGI48_RS16310 0.52 -2.4 3,657,875 + HGI48_RS16310 0.59 +1.2 3,657,876 - -0.4 3,657,899 + +0.0 3,657,900 - HGI48_RS16310 0.64 +0.3 3,657,900 - HGI48_RS16310 0.64 +1.1 3,657,900 - HGI48_RS16310 0.64 +0.2 3,657,902 + HGI48_RS16310 0.65 +0.9 3,657,903 - HGI48_RS16310 0.65 +0.3 3,657,903 - HGI48_RS16310 0.65 +0.6 3,657,918 - HGI48_RS16310 0.69 -0.4 3,657,918 - HGI48_RS16310 0.69 -3.0 3,657,951 + HGI48_RS16310 0.76 -0.2 3,658,061 + +0.2 3,658,061 + -1.4 3,658,062 - +1.6 3,658,062 - -0.2 3,658,072 + -1.4 3,658,078 + -0.4 3,658,078 + -1.4 3,658,154 + HGI48_RS16315 0.19 +0.3 3,658,154 + HGI48_RS16315 0.19 -1.2 3,658,155 - HGI48_RS16315 0.19 -2.4 3,658,192 + HGI48_RS16315 0.25 -2.0 3,658,193 - HGI48_RS16315 0.25 +0.2 3,658,320 + HGI48_RS16315 0.48 -3.0 3,658,320 + HGI48_RS16315 0.48 +0.2 3,658,320 + HGI48_RS16315 0.48 +1.5 3,658,321 - HGI48_RS16315 0.48 +0.2 3,658,321 - +1.9 3,658,356 - -1.0 3,658,356 - HGI48_RS16315 0.54 +0.8 3,658,356 - HGI48_RS16315 0.54 +1.4 3,658,409 - HGI48_RS16315 0.63 +1.3 3,658,441 + HGI48_RS16315 0.69 +0.7 3,658,442 - HGI48_RS16315 0.69 +1.2 3,658,503 + HGI48_RS16315 0.80 -0.6 3,658,504 - HGI48_RS16315 0.80 -1.7 3,658,510 + HGI48_RS16315 0.81 -0.5 3,658,510 + HGI48_RS16315 0.81 -0.6 3,658,511 - HGI48_RS16315 0.81 +1.8 3,658,511 - -1.0 3,658,515 + HGI48_RS16315 0.82 +0.2 3,658,515 + HGI48_RS16315 0.82 +0.4 3,658,515 + HGI48_RS16315 0.82 -2.4 3,658,516 - HGI48_RS16315 0.82 +0.6 3,658,516 - HGI48_RS16315 0.82 +0.6 3,658,611 + +2.5 3,658,611 + -0.4 3,658,612 - -1.2 3,658,612 - +1.6 3,658,648 - -2.4 3,658,712 + -0.9 3,658,781 + -1.0 3,658,843 + HGI48_RS16320 0.65 -1.6 3,658,843 + HGI48_RS16320 0.65 +0.2 3,658,843 + HGI48_RS16320 0.65 -1.9 3,658,843 + HGI48_RS16320 0.65 -0.7 3,658,848 + HGI48_RS16320 0.71 +0.4 3,658,874 + -1.7 3,658,886 - -1.3 3,658,893 + +0.6 3,658,898 + -0.1 3,658,899 - +2.7 3,658,918 + +0.9 3,659,031 + +1.3 3,659,038 + +1.2 3,659,039 - +0.6 3,659,077 - -2.0 3,659,478 + +0.3 3,659,502 - +0.0 3,659,556 - +1.2 3,659,558 + +1.5 3,659,559 - -0.7 3,659,559 - +0.2 3,659,566 + +1.2 3,659,566 + -0.9 3,659,567 - +0.5 3,659,574 - +0.6 3,659,770 - +2.3
Or see this region's nucleotide sequence