Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS16005 and HGI48_RS16010 are separated by 210 nucleotides HGI48_RS16010 and HGI48_RS16015 are separated by 55 nucleotides HGI48_RS16015 and HGI48_RS16020 are separated by 89 nucleotides HGI48_RS16020 and HGI48_RS16025 are separated by 244 nucleotides
HGI48_RS16005: HGI48_RS16005 - dGTPase, at 3,596,302 to 3,597,819
_RS16005
HGI48_RS16010: HGI48_RS16010 - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, at 3,598,030 to 3,598,731
_RS16010
HGI48_RS16015: HGI48_RS16015 - vitamin B12 ABC transporter substrate-binding protein BtuF, at 3,598,787 to 3,599,617
_RS16015
HGI48_RS16020: HGI48_RS16020 - iron-sulfur cluster insertion protein ErpA, at 3,599,707 to 3,600,051
_RS16020
HGI48_RS16025: HGI48_RS16025 - glutamate-1-semialdehyde 2,1-aminomutase, at 3,600,296 to 3,601,576
_RS16025
Position (kb)
3598
3599
3600 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3597.819 kb on + strand at 3597.819 kb on + strand at 3597.819 kb on + strand at 3597.820 kb on - strand at 3597.820 kb on - strand at 3597.820 kb on - strand at 3597.833 kb on + strand at 3597.833 kb on + strand at 3597.833 kb on + strand at 3597.833 kb on + strand at 3597.833 kb on + strand at 3597.833 kb on + strand at 3597.833 kb on + strand at 3597.833 kb on + strand at 3597.833 kb on + strand at 3597.833 kb on + strand at 3597.833 kb on + strand at 3597.834 kb on - strand at 3597.834 kb on - strand at 3597.834 kb on - strand at 3597.834 kb on - strand at 3597.834 kb on - strand at 3597.834 kb on - strand at 3597.834 kb on - strand at 3597.834 kb on - strand at 3597.919 kb on + strand at 3597.919 kb on + strand at 3597.938 kb on + strand at 3597.958 kb on + strand at 3597.958 kb on + strand at 3597.958 kb on + strand at 3597.986 kb on + strand at 3598.830 kb on + strand at 3598.831 kb on - strand at 3598.958 kb on + strand, within HGI48_RS16015 at 3598.959 kb on - strand, within HGI48_RS16015 at 3599.117 kb on + strand, within HGI48_RS16015 at 3599.117 kb on + strand, within HGI48_RS16015 at 3599.249 kb on + strand, within HGI48_RS16015 at 3599.249 kb on + strand, within HGI48_RS16015 at 3599.364 kb on + strand, within HGI48_RS16015 at 3599.364 kb on + strand, within HGI48_RS16015 at 3599.364 kb on + strand, within HGI48_RS16015 at 3599.364 kb on + strand, within HGI48_RS16015 at 3599.365 kb on - strand, within HGI48_RS16015 at 3599.472 kb on + strand, within HGI48_RS16015 at 3599.654 kb on + strand at 3599.675 kb on - strand at 3599.675 kb on - strand at 3599.696 kb on - strand at 3599.696 kb on - strand at 3600.107 kb on - strand at 3600.211 kb on + strand at 3600.211 kb on + strand at 3600.251 kb on + strand at 3600.251 kb on + strand at 3600.252 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,597,819 + +1.6 3,597,819 + -0.4 3,597,819 + +0.3 3,597,820 - -1.7 3,597,820 - +0.6 3,597,820 - -0.1 3,597,833 + -0.7 3,597,833 + +1.5 3,597,833 + -1.9 3,597,833 + +0.2 3,597,833 + +1.8 3,597,833 + -0.6 3,597,833 + -0.2 3,597,833 + +0.2 3,597,833 + +0.3 3,597,833 + -0.0 3,597,833 + -2.4 3,597,834 - -0.2 3,597,834 - +0.2 3,597,834 - -1.7 3,597,834 - +1.6 3,597,834 - -0.0 3,597,834 - +2.2 3,597,834 - -1.7 3,597,834 - -2.0 3,597,919 + +0.2 3,597,919 + +1.8 3,597,938 + +0.8 3,597,958 + -0.3 3,597,958 + +0.9 3,597,958 + +1.2 3,597,986 + +0.4 3,598,830 + -2.0 3,598,831 - +0.2 3,598,958 + HGI48_RS16015 0.21 -0.9 3,598,959 - HGI48_RS16015 0.21 -2.0 3,599,117 + HGI48_RS16015 0.40 -0.1 3,599,117 + HGI48_RS16015 0.40 +0.6 3,599,249 + HGI48_RS16015 0.56 -2.0 3,599,249 + HGI48_RS16015 0.56 -0.2 3,599,364 + HGI48_RS16015 0.69 -0.9 3,599,364 + HGI48_RS16015 0.69 -1.6 3,599,364 + HGI48_RS16015 0.69 +1.6 3,599,364 + HGI48_RS16015 0.69 +0.3 3,599,365 - HGI48_RS16015 0.70 -1.0 3,599,472 + HGI48_RS16015 0.82 +0.5 3,599,654 + -1.4 3,599,675 - -0.2 3,599,675 - +0.7 3,599,696 - +0.1 3,599,696 - +0.6 3,600,107 - +0.1 3,600,211 + -2.0 3,600,211 + +1.2 3,600,251 + -1.4 3,600,251 + -0.6 3,600,252 - +0.8
Or see this region's nucleotide sequence