Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS14275 and HGI48_RS14280 are separated by 16 nucleotides HGI48_RS14280 and HGI48_RS14285 overlap by 4 nucleotides HGI48_RS14285 and HGI48_RS14290 are separated by 114 nucleotides
HGI48_RS14275: HGI48_RS14275 - NADH-quinone oxidoreductase subunit NuoI, at 3,205,138 to 3,205,680
_RS14275
HGI48_RS14280: HGI48_RS14280 - NADH-quinone oxidoreductase subunit NuoH, at 3,205,697 to 3,206,671
_RS14280
HGI48_RS14285: HGI48_RS14285 - NADH-quinone oxidoreductase subunit NuoG, at 3,206,668 to 3,209,394
_RS14285
HGI48_RS14290: HGI48_RS14290 - NADH-quinone oxidoreductase subunit NuoF, at 3,209,509 to 3,210,858
_RS14290
Position (kb)
3206
3207
3208
3209
3210 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3205.686 kb on - strand at 3205.686 kb on - strand at 3205.947 kb on + strand, within HGI48_RS14280 at 3205.948 kb on - strand, within HGI48_RS14280 at 3206.119 kb on + strand, within HGI48_RS14280 at 3206.120 kb on - strand, within HGI48_RS14280 at 3206.399 kb on - strand, within HGI48_RS14280 at 3206.948 kb on + strand, within HGI48_RS14285 at 3207.269 kb on + strand, within HGI48_RS14285 at 3207.419 kb on + strand, within HGI48_RS14285 at 3209.168 kb on + strand at 3209.788 kb on - strand, within HGI48_RS14290
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,205,686 - +2.0 3,205,686 - +0.1 3,205,947 + HGI48_RS14280 0.26 -1.9 3,205,948 - HGI48_RS14280 0.26 -2.3 3,206,119 + HGI48_RS14280 0.43 -2.6 3,206,120 - HGI48_RS14280 0.43 +0.0 3,206,399 - HGI48_RS14280 0.72 -0.6 3,206,948 + HGI48_RS14285 0.10 -3.7 3,207,269 + HGI48_RS14285 0.22 -0.2 3,207,419 + HGI48_RS14285 0.28 -0.3 3,209,168 + -1.2 3,209,788 - HGI48_RS14290 0.21 -2.6
Or see this region's nucleotide sequence