Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS13530 and HGI48_RS13535 are separated by 144 nucleotides HGI48_RS13535 and HGI48_RS13540 are separated by 4 nucleotides HGI48_RS13540 and HGI48_RS13545 are separated by 76 nucleotides HGI48_RS13545 and HGI48_RS13550 are separated by 13 nucleotides
HGI48_RS13530: HGI48_RS13530 - flagellar basal body P-ring formation protein FlgA, at 2,961,690 to 2,962,358
_RS13530
HGI48_RS13535: HGI48_RS13535 - anti-sigma-28 factor FlgM, at 2,962,503 to 2,962,802
_RS13535
HGI48_RS13540: HGI48_RS13540 - flagellar export chaperone FlgN, at 2,962,807 to 2,963,244
_RS13540
HGI48_RS13545: HGI48_RS13545 - flagellar protein FlhE, at 2,963,321 to 2,963,743
_RS13545
HGI48_RS13550: HGI48_RS13550 - flagellar biosynthesis protein FlhA, at 2,963,757 to 2,965,844
_RS13550
Position (kb)
2963
2964 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2962.340 kb on + strand at 2962.356 kb on + strand at 2962.370 kb on - strand at 2962.395 kb on + strand at 2962.395 kb on + strand at 2962.395 kb on + strand at 2962.395 kb on + strand at 2962.395 kb on + strand at 2962.395 kb on + strand at 2962.395 kb on + strand at 2962.396 kb on - strand at 2962.396 kb on - strand at 2962.396 kb on - strand at 2962.396 kb on - strand at 2962.396 kb on - strand at 2962.396 kb on - strand at 2962.400 kb on - strand at 2962.400 kb on - strand at 2962.400 kb on - strand at 2962.400 kb on - strand at 2962.421 kb on + strand at 2962.426 kb on - strand at 2962.439 kb on - strand at 2962.439 kb on - strand at 2962.442 kb on + strand at 2962.442 kb on + strand at 2962.464 kb on + strand at 2962.475 kb on - strand at 2962.499 kb on + strand at 2962.500 kb on - strand at 2962.652 kb on + strand, within HGI48_RS13535 at 2962.652 kb on + strand, within HGI48_RS13535 at 2962.653 kb on - strand, within HGI48_RS13535 at 2962.653 kb on - strand, within HGI48_RS13535 at 2962.686 kb on - strand, within HGI48_RS13535 at 2962.765 kb on + strand, within HGI48_RS13535 at 2962.766 kb on - strand, within HGI48_RS13535 at 2962.769 kb on + strand, within HGI48_RS13535 at 2962.770 kb on - strand, within HGI48_RS13535 at 2962.770 kb on - strand, within HGI48_RS13535 at 2962.770 kb on - strand, within HGI48_RS13535 at 2962.770 kb on - strand, within HGI48_RS13535 at 2962.786 kb on + strand at 2962.787 kb on - strand at 2962.787 kb on - strand at 2962.787 kb on - strand at 2962.801 kb on - strand at 2962.821 kb on - strand at 2962.886 kb on + strand, within HGI48_RS13540 at 2963.037 kb on - strand, within HGI48_RS13540 at 2963.103 kb on + strand, within HGI48_RS13540 at 2963.103 kb on + strand, within HGI48_RS13540 at 2963.103 kb on + strand, within HGI48_RS13540 at 2963.103 kb on + strand, within HGI48_RS13540 at 2963.103 kb on + strand, within HGI48_RS13540 at 2963.104 kb on - strand, within HGI48_RS13540 at 2963.104 kb on - strand, within HGI48_RS13540 at 2963.208 kb on + strand at 2963.238 kb on + strand at 2963.239 kb on - strand at 2963.267 kb on - strand at 2963.290 kb on + strand at 2963.302 kb on + strand at 2963.302 kb on + strand at 2963.303 kb on - strand at 2963.303 kb on - strand at 2963.303 kb on - strand at 2963.303 kb on - strand at 2963.304 kb on + strand at 2963.304 kb on + strand at 2963.304 kb on + strand at 2963.304 kb on + strand at 2963.304 kb on + strand at 2963.304 kb on + strand at 2963.304 kb on + strand at 2963.305 kb on - strand at 2963.305 kb on - strand at 2963.305 kb on - strand at 2963.305 kb on - strand at 2963.305 kb on - strand at 2963.305 kb on - strand at 2963.308 kb on + strand at 2963.308 kb on + strand at 2963.308 kb on + strand at 2963.309 kb on - strand at 2963.311 kb on + strand at 2963.311 kb on + strand at 2963.311 kb on + strand at 2963.318 kb on - strand at 2963.322 kb on + strand at 2963.323 kb on - strand at 2963.326 kb on + strand at 2963.326 kb on + strand at 2963.327 kb on - strand at 2963.328 kb on + strand at 2963.329 kb on - strand at 2963.394 kb on + strand, within HGI48_RS13545 at 2963.394 kb on + strand, within HGI48_RS13545 at 2963.394 kb on + strand at 2963.394 kb on + strand, within HGI48_RS13545 at 2963.394 kb on + strand, within HGI48_RS13545 at 2963.395 kb on - strand, within HGI48_RS13545 at 2963.400 kb on - strand, within HGI48_RS13545 at 2963.400 kb on - strand, within HGI48_RS13545 at 2963.629 kb on - strand, within HGI48_RS13545 at 2963.629 kb on - strand at 2963.657 kb on - strand, within HGI48_RS13545 at 2963.657 kb on - strand, within HGI48_RS13545 at 2963.868 kb on + strand at 2964.006 kb on + strand, within HGI48_RS13550 at 2964.006 kb on + strand, within HGI48_RS13550 at 2964.007 kb on - strand, within HGI48_RS13550 at 2964.007 kb on - strand, within HGI48_RS13550 at 2964.101 kb on + strand, within HGI48_RS13550 at 2964.102 kb on - strand, within HGI48_RS13550 at 2964.124 kb on + strand, within HGI48_RS13550 at 2964.157 kb on - strand, within HGI48_RS13550 at 2964.157 kb on - strand, within HGI48_RS13550 at 2964.179 kb on + strand, within HGI48_RS13550 at 2964.179 kb on + strand, within HGI48_RS13550 at 2964.180 kb on - strand, within HGI48_RS13550 at 2964.204 kb on + strand, within HGI48_RS13550 at 2964.216 kb on + strand, within HGI48_RS13550 at 2964.257 kb on + strand, within HGI48_RS13550 at 2964.257 kb on + strand at 2964.276 kb on - strand, within HGI48_RS13550 at 2964.355 kb on - strand, within HGI48_RS13550 at 2964.383 kb on + strand, within HGI48_RS13550 at 2964.401 kb on + strand, within HGI48_RS13550 at 2964.401 kb on + strand, within HGI48_RS13550 at 2964.401 kb on + strand, within HGI48_RS13550 at 2964.402 kb on - strand, within HGI48_RS13550 at 2964.402 kb on - strand, within HGI48_RS13550 at 2964.402 kb on - strand, within HGI48_RS13550 at 2964.402 kb on - strand, within HGI48_RS13550 at 2964.403 kb on + strand, within HGI48_RS13550 at 2964.403 kb on + strand, within HGI48_RS13550 at 2964.403 kb on + strand, within HGI48_RS13550 at 2964.403 kb on + strand, within HGI48_RS13550 at 2964.403 kb on + strand, within HGI48_RS13550 at 2964.403 kb on + strand, within HGI48_RS13550 at 2964.404 kb on - strand, within HGI48_RS13550 at 2964.404 kb on - strand, within HGI48_RS13550 at 2964.404 kb on - strand, within HGI48_RS13550 at 2964.404 kb on - strand, within HGI48_RS13550 at 2964.404 kb on - strand, within HGI48_RS13550 at 2964.404 kb on - strand, within HGI48_RS13550 at 2964.404 kb on - strand, within HGI48_RS13550 at 2964.404 kb on - strand, within HGI48_RS13550 at 2964.404 kb on - strand, within HGI48_RS13550 at 2964.404 kb on - strand, within HGI48_RS13550 at 2964.581 kb on + strand, within HGI48_RS13550 at 2964.586 kb on + strand, within HGI48_RS13550 at 2964.586 kb on + strand, within HGI48_RS13550 at 2964.586 kb on + strand, within HGI48_RS13550 at 2964.586 kb on + strand, within HGI48_RS13550 at 2964.590 kb on + strand, within HGI48_RS13550 at 2964.621 kb on - strand, within HGI48_RS13550 at 2964.738 kb on + strand, within HGI48_RS13550 at 2964.738 kb on + strand, within HGI48_RS13550 at 2964.738 kb on + strand, within HGI48_RS13550 at 2964.738 kb on + strand, within HGI48_RS13550 at 2964.739 kb on - strand, within HGI48_RS13550 at 2964.739 kb on - strand, within HGI48_RS13550 at 2964.739 kb on - strand, within HGI48_RS13550 at 2964.739 kb on - strand, within HGI48_RS13550
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 2,962,340 + +1.2 2,962,356 + -0.4 2,962,370 - -0.2 2,962,395 + +1.3 2,962,395 + +1.3 2,962,395 + +0.9 2,962,395 + -1.7 2,962,395 + -1.0 2,962,395 + +0.7 2,962,395 + -2.1 2,962,396 - +0.8 2,962,396 - -1.0 2,962,396 - -2.0 2,962,396 - +0.3 2,962,396 - -0.5 2,962,396 - -2.5 2,962,400 - -1.3 2,962,400 - -1.8 2,962,400 - -0.0 2,962,400 - +0.7 2,962,421 + -0.7 2,962,426 - -1.3 2,962,439 - -0.9 2,962,439 - +1.0 2,962,442 + -1.0 2,962,442 + -1.9 2,962,464 + +1.0 2,962,475 - +0.7 2,962,499 + +0.9 2,962,500 - +1.8 2,962,652 + HGI48_RS13535 0.50 -0.3 2,962,652 + HGI48_RS13535 0.50 -0.1 2,962,653 - HGI48_RS13535 0.50 +0.8 2,962,653 - HGI48_RS13535 0.50 +0.7 2,962,686 - HGI48_RS13535 0.61 +0.5 2,962,765 + HGI48_RS13535 0.87 -1.2 2,962,766 - HGI48_RS13535 0.88 -0.5 2,962,769 + HGI48_RS13535 0.89 +0.7 2,962,770 - HGI48_RS13535 0.89 +1.1 2,962,770 - HGI48_RS13535 0.89 -1.0 2,962,770 - HGI48_RS13535 0.89 -0.1 2,962,770 - HGI48_RS13535 0.89 -2.3 2,962,786 + -0.4 2,962,787 - +1.6 2,962,787 - +0.9 2,962,787 - +0.3 2,962,801 - +0.1 2,962,821 - -1.1 2,962,886 + HGI48_RS13540 0.18 -0.4 2,963,037 - HGI48_RS13540 0.53 +1.3 2,963,103 + HGI48_RS13540 0.68 -0.4 2,963,103 + HGI48_RS13540 0.68 -2.0 2,963,103 + HGI48_RS13540 0.68 -2.0 2,963,103 + HGI48_RS13540 0.68 +0.5 2,963,103 + HGI48_RS13540 0.68 +0.1 2,963,104 - HGI48_RS13540 0.68 -0.2 2,963,104 - HGI48_RS13540 0.68 +0.0 2,963,208 + +0.2 2,963,238 + +0.9 2,963,239 - -2.5 2,963,267 - +1.0 2,963,290 + -2.0 2,963,302 + -1.3 2,963,302 + -2.0 2,963,303 - +0.5 2,963,303 - -0.5 2,963,303 - +1.2 2,963,303 - +1.0 2,963,304 + -0.9 2,963,304 + -0.9 2,963,304 + -0.4 2,963,304 + -1.0 2,963,304 + -0.2 2,963,304 + -2.0 2,963,304 + -0.7 2,963,305 - -1.7 2,963,305 - +2.9 2,963,305 - -1.1 2,963,305 - -0.1 2,963,305 - -0.7 2,963,305 - -0.7 2,963,308 + +0.1 2,963,308 + -1.4 2,963,308 + +1.8 2,963,309 - +0.4 2,963,311 + +0.7 2,963,311 + +1.4 2,963,311 + -2.9 2,963,318 - +0.7 2,963,322 + -2.0 2,963,323 - -1.7 2,963,326 + -2.9 2,963,326 + -1.3 2,963,327 - -2.0 2,963,328 + +0.4 2,963,329 - -0.7 2,963,394 + HGI48_RS13545 0.17 +1.0 2,963,394 + HGI48_RS13545 0.17 -0.9 2,963,394 + +1.0 2,963,394 + HGI48_RS13545 0.17 -0.4 2,963,394 + HGI48_RS13545 0.17 -0.7 2,963,395 - HGI48_RS13545 0.17 -2.0 2,963,400 - HGI48_RS13545 0.19 +0.1 2,963,400 - HGI48_RS13545 0.19 +0.3 2,963,629 - HGI48_RS13545 0.73 -0.0 2,963,629 - -1.3 2,963,657 - HGI48_RS13545 0.79 -2.5 2,963,657 - HGI48_RS13545 0.79 -1.0 2,963,868 + +1.7 2,964,006 + HGI48_RS13550 0.12 +0.8 2,964,006 + HGI48_RS13550 0.12 +1.7 2,964,007 - HGI48_RS13550 0.12 +1.3 2,964,007 - HGI48_RS13550 0.12 +0.1 2,964,101 + HGI48_RS13550 0.16 +1.6 2,964,102 - HGI48_RS13550 0.17 +0.6 2,964,124 + HGI48_RS13550 0.18 +1.6 2,964,157 - HGI48_RS13550 0.19 -2.0 2,964,157 - HGI48_RS13550 0.19 +0.5 2,964,179 + HGI48_RS13550 0.20 +1.1 2,964,179 + HGI48_RS13550 0.20 -3.0 2,964,180 - HGI48_RS13550 0.20 +0.5 2,964,204 + HGI48_RS13550 0.21 +0.1 2,964,216 + HGI48_RS13550 0.22 +2.0 2,964,257 + HGI48_RS13550 0.24 -0.1 2,964,257 + +2.3 2,964,276 - HGI48_RS13550 0.25 -0.4 2,964,355 - HGI48_RS13550 0.29 +0.8 2,964,383 + HGI48_RS13550 0.30 +2.3 2,964,401 + HGI48_RS13550 0.31 -0.4 2,964,401 + HGI48_RS13550 0.31 +0.6 2,964,401 + HGI48_RS13550 0.31 +1.2 2,964,402 - HGI48_RS13550 0.31 +1.3 2,964,402 - HGI48_RS13550 0.31 -2.0 2,964,402 - HGI48_RS13550 0.31 -1.7 2,964,402 - HGI48_RS13550 0.31 +1.9 2,964,403 + HGI48_RS13550 0.31 +1.3 2,964,403 + HGI48_RS13550 0.31 +2.6 2,964,403 + HGI48_RS13550 0.31 +2.8 2,964,403 + HGI48_RS13550 0.31 +0.3 2,964,403 + HGI48_RS13550 0.31 +1.9 2,964,403 + HGI48_RS13550 0.31 +0.6 2,964,404 - HGI48_RS13550 0.31 +1.2 2,964,404 - HGI48_RS13550 0.31 -0.5 2,964,404 - HGI48_RS13550 0.31 +0.1 2,964,404 - HGI48_RS13550 0.31 +0.3 2,964,404 - HGI48_RS13550 0.31 +1.0 2,964,404 - HGI48_RS13550 0.31 -0.4 2,964,404 - HGI48_RS13550 0.31 +0.5 2,964,404 - HGI48_RS13550 0.31 +1.4 2,964,404 - HGI48_RS13550 0.31 +0.7 2,964,404 - HGI48_RS13550 0.31 +0.9 2,964,581 + HGI48_RS13550 0.39 +1.7 2,964,586 + HGI48_RS13550 0.40 +1.1 2,964,586 + HGI48_RS13550 0.40 +1.6 2,964,586 + HGI48_RS13550 0.40 +1.5 2,964,586 + HGI48_RS13550 0.40 -0.3 2,964,590 + HGI48_RS13550 0.40 -1.3 2,964,621 - HGI48_RS13550 0.41 +0.7 2,964,738 + HGI48_RS13550 0.47 +1.9 2,964,738 + HGI48_RS13550 0.47 +1.6 2,964,738 + HGI48_RS13550 0.47 +0.0 2,964,738 + HGI48_RS13550 0.47 +2.3 2,964,739 - HGI48_RS13550 0.47 +1.5 2,964,739 - HGI48_RS13550 0.47 +0.5 2,964,739 - HGI48_RS13550 0.47 +0.1 2,964,739 - HGI48_RS13550 0.47 +1.8
Or see this region's nucleotide sequence